41 research outputs found

    Relationship Marketing: Discussion of The Marketing Strategy of China Telecom

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    关系营销是一种经营理念,一种策略指南。关系营销的主要内涵是,关注保持与顾客的关系,高度重视顾客服务,履行高度的顾客承诺和顾客联系,提高企业形象,重视企业品牌效应,同时,企业的所有部门都来关心质量,努力与顾客保持长期、双向、维系不散的友好关系。这样,不断赢得客户、赢得市场,不断发展壮大。关系营销本质上代表了一种典型的营销转变——由以获取/交易为中心到以保留/关系为中心的转变。 在电信市场竞争愈演愈烈的今天,谁的服务好,谁的顾客忠诚度高,谁就能在市场竞争中稳操胜券。提高顾客忠诚度,实施顾客关系营销对中国电信集团战略目标的实现具有重要意义。首先,实施顾客关系营销战略,提高顾客忠诚度是电信市场竞争发...The Relationship Marketing strategy means to develop face-to-face marketing, friendship marketing and future benefit marketing. To improve the customer’s faithfulness, the enterprises have to be concerned about the product quality, fulfilling promises, keeping long-time contact with the customers. For the sake of defeating the opponents and enhancing the marketing shares, keeping loyal customers ...学位:工商管理硕士院系专业:管理学院工商管理教育中心_工商管理硕士(MBA)学号:20001508

    Influence on the morphology and blood flow volume of the gastric mucosa in rats with acute gastric ulcer treated by moxibustion

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    目的:研究艾灸对急性胃溃疡大鼠胃黏膜组织形态的变化和血流量的影响,探讨艾灸促进胃黏膜损伤修复的生物学机制。方法:将大鼠随机分为正常组、模型组、胃经穴组和对照点组,采用束缚冷应激法制作急性胃溃疡大鼠模型,光镜下观察大鼠胃黏膜溃疡指数,多导生理记录仪检测大鼠胃黏膜血流量。结果:与模型组比较,胃经穴组大鼠的胃黏膜溃疡指数值均降低,胃黏膜形态学明显改善,胃黏膜血流量显著增加(P<0.01);与对照点组比较,胃经穴组大鼠胃黏膜溃疡指数、形态学和血流量改善更为明显(P<0.01)。结论:艾灸可增加胃黏膜血流量,促进胃黏膜的损伤修复,并且存在一定的经脉脏腑相关性。Objective: To study the biological repair mechanisms of gastric mucosa damage, through the effect of morphology and blood flow volume of gastric mucosa in rats with acute gastric ulcer treated by moxibustion.Methods: rats were randomly divided into normal group, model group, stomach meridian group, control point group.Except for the normal group, all the rats were established into the gastric mucosa injury model by cold restrain stress.Gastric mucosal injury index in rats wereobserved by light microscopy, the gastric mucosal blood flow volume in rats were tested by the physiological recorder detection.Results: Compared with the model group, the gastric mucosa ulcer index was decreased, the gastric mucosa morphology was significantly improved, gastric mucosal blood flow volume was significantly increased in stomach meridian group(P<0.01).The gastric mucosa ulcer index, gastric mucosa morphology and blood flow volume in stomach meridian group were improved significantly than those in control point group(P<0.01).Conclusion: Moxibustion on the stomach meridian could increase the gastric mucosal blood flow volume, promote the repair of gastric mucosa injury, and there is a certain correlation between meridians and viscera.国家自然科学基金项目(No.30960484;No.81260556)~

    Effects of Moxibustion on Gastric Stress Ulcer Rats' Apoptosis Protein Phosphorylation

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    目的:研究艾灸对应激性胃溃疡大鼠胃黏膜细胞凋亡蛋白质磷酸化的影响,探讨艾灸促进胃黏膜损伤修复的信号转导机制。方法:将大鼠随机分为正常组、模型组、胃经穴组和对照点组,采用束缚冷应激法制作应激性胃溃疡大鼠模型,肉眼观察大鼠胃黏膜损伤程度,APOPTOSIS MICrOArrAy SlIdES芯片检测胃黏膜细胞凋亡蛋白质磷酸化水平。结果:与模型组比较,胃经穴组和对照点组大鼠胃黏膜损伤指数值均显著降低(P<0.05);与对照点组比较,胃经穴组大鼠胃黏膜损伤指数显著降低(P<0.05);APOPTOSIS MICrOArrAy SlIdES芯片检测结果显示:与模型组比较,胃经穴组大鼠胃黏膜细胞10种蛋白质磷酸化水平上调,其中bCl-Xl、MCl-1、bCl-2、IAPS 4种蛋白磷酸化水平差异有统计学意义(P<0.01,P<0.05);18种蛋白质磷酸化水平下调,其中Tnf、fAS、APAf-1、CASPASE-3、CASPASE-9、bAX 6种蛋白质磷酸化水平差异有统计学意义(P<0.01,P<0.05)。结论:艾灸可促进胃黏膜的损伤修复,调节多种凋亡相关信号蛋白质的磷酸化水平,并且存在一定的经脉脏腑相关性。Objective: To study the effects of moxibustion on apoptosis protein phosphorylation in rats with gastric stress ulcer and to explore the signal transduction mechanisms promoting gastric mucosal injury and repairing moxibustion.Methods: The rats were randomly divided into normal group,model group,stomach meridian group and the control point groups and each group had 10 rats.The stress ulcer rat model was established by using restraint cold stress method.We observed the rat gastric mucosa injury degree and Apoptosis Microarray Slides microarray was used to observe the gastric apoptosis protein phosphorylation levels.Results: Compared with the model group,the gastric mucosa injury index values of control point and meridian groups were significantly lower( P < 0.05).Compared with the control point group,a significant reduction in rat gastric mucosa injury index was in meridian group( P < 0.05).Apoptosis Microarray Slides microarray results showed that 10 kinds of protein phosphorylation levels increased,among which Bcl- XL,Mcl- 1,Bcl-2 and IAPs protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05) and 18 kinds of protein phosphorylation levels decreased,among which TNF,Fas,Apaf- 1,Caspase- 3,Caspase- 9 and Bax protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05).Conclusion: Moxibustion can promote gastric mucosal injury and repair and regulate phosphorylation of several proteins related to apoptotic signals and there is a certain correlation between the meridians organs.国家自然科学基金项目(30960484;81260556

    一种基于脉冲激发的光释光测控装置

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    本发明公开一种基于脉冲激发的光释光测控装置。它包括脉冲激发光源、样品盘、光电倍增管,总信号接收控制处理模块,光源控制模块,所述的脉冲激发光源的出光口与样品盘的进光口通过光纤相连,样品盘的出光口与光电倍增管的通过光纤相连,总信号接收控制处理模块分别与光电倍增管和光源控制模块信号连接;所述的总信号接收控制处理模块是:a、发出信号给光源控制模块对激发光源进行控制;b、接受光电倍增管的信号,并对光电倍增管进行控制,对收到的信号数据进行分析处理。本发明具有光源可调整、脉冲发光、扩展性强、易于控制、提高工作效率和减少实验准备时间和材料浪费的优点

    Research on the Effect of Moxibustion on Gastric Mucosal Injury Repair Related Cell Signaling Proteins with Protein Fingerprinting

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    目的研究艾灸对胃黏膜损伤修复相关细胞信号蛋白质的影响,探讨艾灸促进胃黏膜损伤修复的分子机制。方法40只Sd大鼠随机分为正常组、模型组、胃经穴组和对照点组各10只,采用束缚冷应激法建立应激性胃溃疡大鼠模型,胃经穴组大鼠造模后鼠板束缚,艾灸胃经“梁门“、“足三里“穴位;对照点组鼠板束缚,艾灸对照点;空白组和模型组仅鼠板束缚,各组均每次20MIn,每日1次,持续12天。应用蛋白质指纹图谱技术和疏水芯片、亲水芯片、金属离子螯合芯片检测各组大鼠胃黏膜细胞蛋白质指纹图谱,对比分析各组的差异蛋白质质荷比峰。结果各组蛋白质指纹图谱比较,3个芯片共检测出10个标志蛋白质差异表达,其中疏水芯片有3个标志蛋白质表达差异,分子量分别是2736dA、6985dA、7324dA;亲水芯片有1个标志蛋白质表达差异,分子量是4561dA;金属离子螯合芯片有6个标志蛋白质表达差异,分子量分别是1034dA、2136dA、3261dA、4871dA、7586dA、8267dA。结论艾灸可调节胃黏膜损伤模型大鼠多种相关信号蛋白质的差异表达,这可能是其促进胃黏膜损伤修复的分子机制之一。Objective To study the effect of moxibustion on gastric mucosal injury repair related cell signaling proteins and its molecular mechanism.Methods Forty SD rats were randomized into the normal(blank)group,model group,stomach channel point group and control point group,with 10in each.The stress ulcer rat models were established with restraint cold stress method.The stomach channel point group was given moxibustion on Liangmen(ST21)and Zusanli(ST36).The control point group was given moxibustion on the control points.The rats in the blank and model groups were tied with mouse board only.Each group was given corresponding measures 20min each time and once daily for 12days.The mucosal cell protein fingerprint was detected with protein fingerprinting,hydrophobic chip,hydrophilic chip and metal ion chelating chip.The mass-to-charge ratio of differential protein in each group was compared and analyzed.Results Comparison of protein fingerprints in each group showed that 10signs of differentially expressed proteins were detected in 3chips.Three signs of differentially expressed proteins were detected in hydrophobic chip and their molecular weights were 2736Da,6985Da and7324Da.One sign of differentially expressed protein was detected in hydrophilic chip and its molecular weight was 4561Da.Six signs of differentially expressed proteins were detected in metal ion chelating chip and their molecular weights were 1034Da,2136Da,3261Da,4871Da,7586Da and 8267Da.Conclusion Moxibustion can regulate the differential expression in a variety of related signaling proteins and promote the repairation of gastric mucosal injury.国家自然科学基金资助项目(30960484;81260556); 江西省自然(青年)科学基金资助项目(2008GZY0022); 江西省教育厅2009年度科技计划资助项目(GJJ09280

    Effects of drying and re-watering on the photosynthesis and active oxygen metabolism of Periploca sepium seedlings

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    采用人工控制土壤水分试验,以80%田间持水量处理为对照,研究了3次干旱-复水处理对2年生杠柳幼苗叶片光合作用,根、茎、叶膜脂过氧化和抗氧化系统的影响.结果表明:在干旱条件下,杠柳叶片相对含水量和光合速率(Pn)显著降低,光合色素含量升高.干旱复水后,叶片相对含水量完全恢复,反映了杠柳较强的旱后修复能力;叶绿素含量和Pn均明显高于对照,表现出补偿效应,适度干旱诱导了杠柳的抗旱适应性.干旱胁迫使Pn下降,以气孔限制为主,但在中午发生了非气孔限制;幼叶、新茎和细根中的超氧阴离子产生速率升高,丙二醛含量则降低,说明这些幼嫩组织未受到氧化伤害.不同器官中SOD、CAT和POD活性及变化趋势不同,以细根的3种保护酶的反应最为灵敏,说明细根是杠柳适应干旱环境的重要器官.器官间的相互合作与协调使杠柳能有效地适应干湿交替的干旱环境
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