目的:研究艾灸对应激性胃溃疡大鼠胃黏膜细胞凋亡蛋白质磷酸化的影响,探讨艾灸促进胃黏膜损伤修复的信号转导机制。方法:将大鼠随机分为正常组、模型组、胃经穴组和对照点组,采用束缚冷应激法制作应激性胃溃疡大鼠模型,肉眼观察大鼠胃黏膜损伤程度,APOPTOSIS MICrOArrAy SlIdES芯片检测胃黏膜细胞凋亡蛋白质磷酸化水平。结果:与模型组比较,胃经穴组和对照点组大鼠胃黏膜损伤指数值均显著降低(P<0.05);与对照点组比较,胃经穴组大鼠胃黏膜损伤指数显著降低(P<0.05);APOPTOSIS MICrOArrAy SlIdES芯片检测结果显示:与模型组比较,胃经穴组大鼠胃黏膜细胞10种蛋白质磷酸化水平上调,其中bCl-Xl、MCl-1、bCl-2、IAPS 4种蛋白磷酸化水平差异有统计学意义(P<0.01,P<0.05);18种蛋白质磷酸化水平下调,其中Tnf、fAS、APAf-1、CASPASE-3、CASPASE-9、bAX 6种蛋白质磷酸化水平差异有统计学意义(P<0.01,P<0.05)。结论:艾灸可促进胃黏膜的损伤修复,调节多种凋亡相关信号蛋白质的磷酸化水平,并且存在一定的经脉脏腑相关性。Objective: To study the effects of moxibustion on apoptosis protein phosphorylation in rats with gastric stress ulcer and to explore the signal transduction mechanisms promoting gastric mucosal injury and repairing moxibustion.Methods: The rats were randomly divided into normal group,model group,stomach meridian group and the control point groups and each group had 10 rats.The stress ulcer rat model was established by using restraint cold stress method.We observed the rat gastric mucosa injury degree and Apoptosis Microarray Slides microarray was used to observe the gastric apoptosis protein phosphorylation levels.Results: Compared with the model group,the gastric mucosa injury index values of control point and meridian groups were significantly lower( P < 0.05).Compared with the control point group,a significant reduction in rat gastric mucosa injury index was in meridian group( P < 0.05).Apoptosis Microarray Slides microarray results showed that 10 kinds of protein phosphorylation levels increased,among which Bcl- XL,Mcl- 1,Bcl-2 and IAPs protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05) and 18 kinds of protein phosphorylation levels decreased,among which TNF,Fas,Apaf- 1,Caspase- 3,Caspase- 9 and Bax protein phosphorylation levels were statistically significant( P < 0.01,P < 0.05).Conclusion: Moxibustion can promote gastric mucosal injury and repair and regulate phosphorylation of several proteins related to apoptotic signals and there is a certain correlation between the meridians organs.国家自然科学基金项目(30960484;81260556
