Analysis of protein of velvet antler of Cervus Nippon Temminck(VACNT) by deferent manufacture methods

Abstract : Velvet antler of cervus nippon Temminck(VACNT) . were prepared by freeze drying or freeze fresh and fracturing to powdered particles. Extract by buffer, and quantity was mensurated of protein. Separating by columniation layer of CM-32 and G100. determine by SDS polyacrylamide gel electrophoresis. It was find that different manufacture by freeze drying or freeze fresh on VACNT, it’s higher molecular elements as is protein was more than classical methods by boiling water. .New method of manufacture will find some new growth factors , that is very important substances on bio activate , like NGFs and so o

An Activity-maintaining Protein Extraction Method for Bioactive Assay and Proteome Analysis of Velvet Antlers

An Activity-maintaining Protein Extraction Method for Bioactive Assay and Proteome Analysis of Velvet Antler

一种超临界流体萃取-高效液相色谱联用系统

本发明涉及超临界流体萃取装置和高效液相色谱，具体地说是一种超临界流体萃取-高效液相色谱联用系统，包括超临界流体萃取装置、第一号三通阀、第二号三通阀、捕集柱、液相色谱泵、分析柱及检测器；超临界流体萃取装置与第一号三通阀的一个接口相连，而第一号三通阀的另两个接口分别与捕集柱的入口端及液相色谱泵相连，捕集柱的出口端与第二号三通阀的一个接口相连，而第二号三通阀的另两个接口分别与分析柱的入口端及废液瓶相连，分析柱的出口端与检测器相连。本发明利用超临界流体萃取装置实现样品中活性成分的提取，通过高效液相色谱实现在线分离分析，所需样品量小、避免样品污染和变质、分析速度快、操作简单、人为误差小、自动化程度高。带填

基于在线二维色谱的不同生长时期鹿茸的比较蛋白质组分析

建立了基于在线二维弱阴离子交换色谱-反相液相色谱（2D-WAX-RPLC）的蛋白质分离系统,并用于不同生长时期鹿茸的比较蛋白质组分析。以5种标准蛋白质的混合物为样品,考察了系统的重现性。通过改变标准蛋白质之间的浓度比,研究了该系统进行蛋白质相对定量的能力。在此基础上,针对4个不同生长时期的鹿茸样品,分别采用5种不同的方法进行蛋白质提取,经2D-WAX-RPLC分离后,收集各生长时期对应蛋白质的峰高最大比超过2的组分。酶解后,采用微柱反相液相色谱-串联质谱（μRPLC-MS/MS）进行肽段的分离鉴定;共从9个差异蛋白质峰中鉴定到了22个差异蛋白质。研究结果表明,利用基于蛋白质水平的在线二维液相色谱分离技术找寻差异蛋白质,具有重现性好、自动化程度高等优点,可用于开展比较蛋白质组学的研究

A sequential protein extraction strategy for analysis of deer antler proteome and biological activity

A sequential protein extraction strategy for analysis of deer antler proteome and biological activit

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies