Marketing Strategy Analysis Of "ZiYouYiZu" Project Of QuanZhou Post

随着近年来我国经济的快速发展和老百姓生活水平的不断提升，中国汽车保有量保持年均两位数的旺盛增长态势，截止2011年8月底，我国汽车保有量首次突破1亿辆大关，位居世界第二，正式步入世界一流汽车大国的行列。伴随着汽车时代的到来，中国汽车服务行业迎来了巨大的发展商机。 中国邮政为积极介入汽车服务市场，通过企业内外部服务资源，充分发挥邮政“三流合一”的优势，推出了“自邮一族”服务产品，为广大车主提供一体化会员服务。 本文从我国汽车服务市场现状分析入手，全面剖析了泉州邮政“自邮一族”项目面临的内外部竞争环境，探讨了泉州邮政“自邮一族”项目的目标客户定位和应采取的市场营销策略。 本文主要包括6章： ...In recent years, with the rapid development of China’ economy and the increasing improvement of people’s living standards, China’s car ownership has been growing at a double-digit rate. At the end of August 2011, China’ car ownership for the first time exceeded 100 million, ranking the second in the world, which means that China is stepping into the ranks of the world- class automobile powers. Alo...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：X200615508

转型期的中国劳动力参与率

经历过改革开放的风风雨雨,中国的经济取得了迅猛发展,而在推动发展的关键因素中,人力资源起着至关重要的作用。衡量人力资源指标的一个依据是劳动力参与率,文章通过了解处于转型期的中国的劳动力参与率的现状及其成因,提出相应的看法或者解决之道,进而更深入地了解中国的人口红利对经济发展的影响

五氯化磷辅助下氨基酸的自组装成肽反应研究

L α 氨基酸和D α 氨基酸可与五氯化磷直接发生磷酰化反应 ,随后自组装成多肽 ,但 β 氨基酸不能成肽 ,DL α 氨基酸成肽困难 ;在SOCl2 存在下 ,α 氨基酸也不能成肽 .用电喷雾质谱研究了氨基酸的自组装反应 .反应过程中有五元环状的氨基酸五配位磷中间体生成 ,使用硅烷基保护的氨基酸 ,在 3 1PNMR中可观察到五配位磷中间体

MOLECULAR CLONING AND CHARACTERIZATION OF STEVIA REBAUDIANA UDP-GLUCOSYLTRANSFERASE

本文对一种新的甜菊糖基转移酶进行了基因克隆和功能分析。获得的基因cDNA全长1419bp,编码473个氨基酸,蛋白质分子量约53.2K Da。与常见的糖基转移酶基因比较,相似性达44%以上,且具有糖基转移酶的保守序列。体外异源表达获得的融合蛋白,具有在花青素类和甜菊醇等糖基受体上转糖基的酶活性。在对一系列不同底物的酶活性进行比较后,推测这种糖基转移酶在体内参与了甜菊糖苷的合成。结果表明,具有广泛的底物活性的类黄酮类糖基转移酶,在甜菊体内不仅对类黄酮转糖基,而且在生成水溶性甜菊糖苷的过程中也扮演重要的角色。We report here the cloning and characterization of a UDP-glucose flavonoid glucosyltransferase (srUFGT) in Stevia rebaudiana. The isolated cDNA was 1419bp in length encoding 473 deduced amino acids with a predicted molecular mass of 53. 2 kDa. The products of in vitro translation from an expression vector had anthocyanidins and steviol glucosyltransferase activity. Comparison of the activity of the recombinant UDP-glucosyltransferase toward a range of acceptor substrates suggests that it may participate in the synthesis of steviol glycosides. The results support the hypothesis that the flavonoid glucosyltransferases, which have a broad substrate specificity,may be not only involved in flavonoid glucosylation but also play a role in producing the water-soluble steviol-glycosides in S. rebaudiana

Cloning and Sequencing of the cDNA of UDPG-glucosyltransferase from Stevia rebaudiana

利用与植物次生代谢产物糖基化相关的UDPG糖基转移酶的特征性保守区域 ,设计了同源简并引物 ,以甜菊基因组DNA为模板 ,PCR扩增获得甜菊糖基转移酶基因片段 .根据获得的基因片段设计引物GSTr和GSTf,分别与cDNA文库的T3 和T7通用引物扩增获得全长核苷酸序列的甜菊糖基转移酶基因 ,定名为sudpt 2 ,该基因全长 16 6 2bp ,包括poly(A)和一个 136 2bp的开放阅读框 ,编码 45 4个氨基酸 .与常见的糖基转移酶基因的相似性达 44 %～ 77% ,且具有UDPG糖基转移酶的特征性保守序列 .分子发育进化分析表明 ,此基因为类黄酮糖基转移酶基因家族成员By using two homologous degenerated primers, DNA fragments encoding steviol UDPG glucosyltransferase were amplified from the leaves of Stevia rebaudiana. The DNA fragment with strong homology to known flavonoid glucosyltransferases was used to design two primers to amplify upstream and downstream cDNA of stevia glucosyltransferase. The cDNA of stevia glucosyltransferase sudpg-2 was 1 662 bp in length, with a poly(A) tail and a 1 362 bp open reading frame which encoded 454 deduced amino acid residues. The deduced amimo acids sequence has 40%～60% identity to other plant glucosyltransferase and includes a part of the glucosyltransferase signature sequence. Molecular phylogenic relations of the deduced amino acids sequences with other plant glucosyltransferase suggested that the steviol UDPG glucosyltransferase belongs to family of flavonoid glucosyltransferases

Polar microorganisms,a potential source for new natural medicines-A review

通讯作者。Tel: +86-592-2183217; Fax: +86-592-2184528; E-mail: [email protected] 作者简介: 曾胤新(1971− ), 男, 重庆人, 在职博士研究生, 副研究员, 研究方向为极地及海洋微生物学。E-mail: [email protected][中文文摘] 微生物是极地生态系统的重要组成部分。由于极地独特的地理、气候及环境特点,极地微生物所具有的新颖性与多样性,在科学研究、应用开发等方面都具有重要价值,并逐渐受到人们的广泛关注。有关极地微生物的资源勘探与代谢活性产物研究,已成为国际微生物学研究的热点之一。结合笔者的工作实践,对近年来极地微生物资源的勘探与收集情况进行了介绍,并着重对以医药与生物农药等为目标的极地微生物产活性化合物的研究现状进行了概述,同时还对目前一些影响研究工作开展的限制因素进行了讨论。可以预见,针对极地微生物资源的研究开发工作,将会给新型天然药物的筛选与发现带来新的机遇与突破。[英文文摘] Microorganisms are an important component of polar ecosystems.Based on the unique features of geographical location,climate and environment of polar regions,the novelty and biodiversity of polar microorganisms have attracted an increasing attention for their significant values in both scientific research and resources exploitation.Bioprospecting and natural products research of polar microorganisms have become a hot field of microbiology.In this review,following the introduction of bioprospecting and culture collection of polar microorganisms in recent years,the research and develop-ment of microbial bioactive compounds aimed for pharmaceuticals and biological agriculture chemicals are summarized.In addition,factors that currently limit the research progress are discussed.It is believed that the resources exploitation of polar microorganisms can provide an opportunity for discovery of new natural medicines.Compared to human pharma-ceuticals,the research and development of biological agriculture chemicals has more possibilities to achieve a break-through in a short period of time.国家自然科学基金(40676002);国家“973项目”(2004CB719601

Trace Element Determination in Teas and Discrimination Analysis for Teas

应用微波消解处理样品,电感耦合等离子体质谱法测定,考察了不同产地、不同种类的29种茶叶中的Mg、A l、P、Ca、Cr、Mn、Fe、Co、N i、Cu、Zn、Sr和Pb共13种元素的含量。原始数据经过标准化处理后,结合聚类分析和主成分分析,对来自江西、云南、广东和福建四个地区的茶叶进行了产地判别。对不同种类的茶叶(红茶、绿茶、乌龙茶、黑茶)也进行了区分,结果令人满意。茶叶中矿质元素的含量可做为茶叶产地判别的测量指标之一。The content of mineral elements in 29 tea samples from different areas(Jiangxi,Yunnan,Guangdong and Fujian),including green,black,oolong and dark tea,were analyzed.Mg,Al,P,Ca,Cr,Mn,Fe,Co,Ni,Cu,Zn, Sr,Pb were determined by ICP-MS after digesting by microwave digestion oven.Cluster analysis and principal component analysis(PCA) were applied to differentiate the tea varieties and their geographical origin.The classification performance was fair for these two methods.The content of the mineral elements in teas were good chemical descriptors for differentiating their geographical origins.山东省自然科学基金项目(Q2004B02

Medium Optimization for β-1,3-1,4-Glucanase Production by Recombinant Escherichia coli

为了提高重组大肠杆菌(ESCHErICHIA COlI)rOSETTA(dE3)-PET-22-bgl-(kIl-kM)的产酶能力,采用单因素试验研究了培养基碳源、氮源及碳氮比(摩尔比)对重组大肠杆菌rOSETTA(dE3)-PET-22-bgl-(kIl-kM)分泌表达重组β-1,3-1,4-葡聚糖酶H(A107-M)的影响.在此基础上,采用bOX-bEHnkEn设计法和响应面分析法对以上影响因素进行优化,得出最优培养基成分为(g/l):甘油10.01,酪蛋白胨12.03,酵母粉24,nACl 10,(nH4)2SO44.77,kH2PO42.31,k2HPO412.54.在In order to improve the enzyme activity of Escherichia coli Rosetta(DE3)-pET-22-bgl-(kil-Km),the effects of carbon sources,nitrogen sources and C/N ratio on the production of β-1,3-1,4-glucanase H(A107-M) by E.coli Rosetta(DE3)-pET-22-bgl-(kil-Km) were investigated by one-factor-a-time experiment.Box-Behnken design and a response surface methodology(RSM) were further used to optimize the above critical factors for enzyme production.The optimized medium contained(g/L):glycerol 10.01,casein peptone 12.03,yeast 24,NaCl 10,(NH4)2SO4 4.77,KH2PO4 2.31,and K2HPO4 12.54.When the recombinant E.coli was cultivated in the optimized medium for 27 h,the enzyme activity of β-1,3-1,4-glucanase H(A107-M) reached 78.32 U/mL(lichenan as the substrate),which was about 2.84 times of which obtained in the initial medium.科技部科研院所技术开发研究专项资金(NCSTE-2007-JKZX-023