Research and Fabrication of Opto-mechanical Systems of New UV Spectrophotometer

光谱分析检测技术和光谱仪器经历了近百年的发展，在现代科学实验、生物研究、医学及医药研究、工农业生产、国防、天文观测等领域得到了极其广泛的应用。随着现代科学技术的飞速发展，作为光谱仪中的一种，传统的紫外分光光度计采用光电倍增管作为光电接收器件，并配以波长扫描机构实现波长扫描，该类仪器结构复杂、体积大、光谱扫描时间长，己经不能满足当前发展的需要。许多研究、应用领域对分光光度计提出了微小型化、快速化、使用方便灵活、性能价格比高等更高要求。 本论文研制了一款一次性全光谱测量的新型紫外分光光度计。与传统的紫外可见分光光度计相比，本研制的新型的紫外可见分光光度计具有以下独特的优点： 1)采用CCD为光...Spectrum analysis-detecton and spectrometer has been widely used in many fields, such as modern scientific experiments, biological research, medicine and pharmacy, industrial and agriculture production, national defense and astronomical observation. With the development of the modern techniques, as one of spectrometer, in the traditional UV spectrophotometers PMTs are used as the receiver, and a ...学位：工学硕士院系专业：物理与机电工程学院物理学系_精密仪器及机械学号：1992006115185

Study on spare part controlling system in D company

随着IT产品的同质化,售后服务在IT企业之间的竞争中的地位越来越显得重要,售后服务备件的库存在很大程度上决定了服务成本和总成本，又制约了服务的响应速度和服务承诺的兑现，是IT企业经营管理中十分重要的内容。 备件库存控制是基于企业战略之上，在给定服务目标的条件下，确定所需的最低服务总成本。企业追求的是在给定客户服务水平下使服务总成本最小化。而服务备件成本占服务总成本中较大的比率，它包括存货成本、订货成本、缺货成本、购买成本。 本文内容主要分为五章.第一章综述阐述了本文的研究背景及意义，第二章介绍了服务备件管理的特点及方法，第三章和第四章为本文的中心，第三章介绍了D公司当前备件管理模式所采用的...After-sale service is more important in current market competition, The spare part for service is a key factor of after-sale service’ quality , The spare part inventory influence service cost, service response rate and customer’s satisfaction. What the company pursue is to reduce the cost under certain service goal, Spare part inventory cost take great proportion of total service cost. ...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：20041511

Status of Mud Crabs(Scylla spp.) Farming and Studies on the Population Biology of S. paramamosain

综述了国内外青蟹养殖的基本情况,包括养殖模式、养殖历史、人工育苗的现状,我国青蟹养殖在海水养殖中的地位及其特点,以及我国拟穴青蟹种群生物学研究现状及其在养殖中的重要性.This paper summarized the internationally general situation of mud crabs( Scylla spp.) farming,including cultivation pattern,cultural history,the situation of artificial mass larvae culturing,study progresses of population biology of S. paramamosain and its significance in its farming in China,as well as the status and characteristics of China mud crab farming.国家自然科学基金(31472294

磁共振成像中背景不均匀场去除方法研究

磁敏感加权成像(susceptibility weighted imaging,SWI)是近年来新开发的磁共振成像技术,最早由E.Mack Haacke等于1997发明[1],并最初被称作"高分辨率血氧水平依赖静脉成像"(high resolution blood oxygenation level dependent venographic imaging)。中央高校基本科研业务费专项资金(2010121101);国家自然科学基金(81171331

Studies on the Interactions between Bioactive Peroxovanadium Complexes Bearing Organic Ligands and Histidine

在合成和表征了 4种具有较强生物活性双过氧钒配合物K3 [VO(O2 ) 2 (ox) ]·2H2 O ,Na[VO(O2 ) 2 (bipy) ]·5H2 O ,K[VO(O2 ) 2 (phen) ]·3H2 O和K2 [VO(O2 ) 2 (pic) ]·2H2 O [分别缩写为pV(ox) ,pV(bipy) ,pV(phen)和pV(pic) ,其中ox为草酸根 ,bipy为 2 ,2′ 联吡啶 ,phen为邻菲咯啉 ,pic为 2 羧酸吡啶负离子 ]的基础上 ,利用多种NMR技术和电喷雾质谱 (ESI MS)研究了这 4种含有机配体双过氧钒配合物与组氨酸 (His)在溶液中的相互作用以及反应物浓度、时间、pH等对相互作用体系的影响 .51VNMR跟踪监测的结果表明 :双过氧钒配合物pV(ox)和pV(pic)与His在中性水溶液中存在强配位相互作用 ,而pV(bipy)和pV(phen)与His在中性水溶液中无明显作用 .我们还利用谱学方法确定了pV(ox)和His相互作用后所生成产物是pV(ox)分别与His咪唑基上的 3 N和 1 N配位的一对异构体. 　In order to explore the structure2activity relationship and molecular mechanism of the specific recognition between peroxovanadium (pV) complexes bearing organic ligands and the target enzymes of tyrosine phosphatase , several NMR techniques and ESI2MS were used to study the interactions of four pV complexes {pV(ox) , pV ( bipy) , pV (phen) and pV (pic) , where pV = [VO (O2 ) 2L ] n - , in which L = oxalic acid dianion (ox) , bipyridine (bipy) , 1 ,102phenanthroline (phen) , and pyridine222carboxylic acid (pic) } towards histidine. Strong coordination interactions between imidazole of histidine and vanadium of pV(ox) or pV(pic) were observed in neutral solution , while there are not obvious interactions between histidine and pV(bipy) or pV(phen) . All 13C and 1H NMR signals of 1∶1 stoichiometric mixture of pV(ox) and histidine were assigned. Spectroscopic studies demonstrated that new complexes in the mixture of pV(ox) and histidine are a pair of isomers in which the vanadium in pV(ox) binding to the 32N and 12N of the imidazole ring. Moreover , the results of effective factors on the interaction system indicated that the new isomers were stable under the condition of physiological pH and the structure2activity relationship of these pV complexes may be relevant to their specific recognition towards histidine residues in tyrosine phosphatase.国家自然科学基金 (Nos.2 0 172 0 42 ,2 9832 0 2 0 )资助项

Application of Vector Frequency Converters in Tea Baking Time Control

[中文文摘]茶叶的烘焙时间控制存在的问题主要是:茶叶的含水量与烘焙时间没有建立准确的控制模型;各烘焙箱之间的连动不能统一控制;茶叶品种与烘焙时间关系没有进行记录,造成产品质量的重复性差。对传动控制系统进行技术改造;采用矢量变频器对烘焙箱传送带电动机实现无速度传感器控制;采用基于RS485总线的网络对变频器进行控制;建立烘焙箱之间的连动控制模型;建立烘焙时间与变频器给定频率之间的数学模型;工控机把控制参数自动存入数据库并完成报表打印、变频器参数设置和存储。现场应用表明该技术改造有效可行。[英文文摘]Main problems for tea baking time control are that the control model between the moisture content of tea and the baking time was not built; baking boxes were not centralized automatically controlled; the baking time had no tracking record. It is improved upon the gearing: controlled transport electromotors based on vector control frequency converters(VCFC), controlled centralized automatically VCFC based on the RS485 bus, built a control model for baking boxes, built a mathematical model between the bakingtime and the frequency of VCFC The computer saved control parameters to the database and printed reports The results show that the improvement is effective and feasible.福建省产学研究项目(0041-K81035

硅藻对不同生态习性桡足类抗氧化相关基因表达的影响

采用实时荧光定量PCR(qRT-PCR)技术比较了不同生态习性的太平洋纺锤水蚤(Acartia pacifica)、安氏伪镖水蚤(Pseudodiaptomus annandalei)和日本虎斑猛水蚤(Tigriopus japonicas)摄食不同浓度的牟氏角毛藻(Chaetoceros muelleri)和小新月菱形藻(Nitzschia closterium f.minutissima)两种硅藻后,其MnSOD(锰超氧化物歧化酶)和GST(谷胱甘肽转移酶)两种抗氧化相关基因表达水平的变化情况。结果显示:低浓度的硅藻对哲水蚤类的太平洋纺锤水蚤和安氏伪镖水蚤抗氧化基因表达水平表现出显著的诱导效应,而高浓度的硅藻则表现为抑制效应,不论是低浓度还是高浓度的硅藻均能诱导猛水蚤类的日本虎斑猛水蚤抗氧化基因的表达,表明不同类别的桡足类对硅藻的分子响应存在物种特异性。为评估哲水蚤桡足类对硅藻的影响是否具有适应能力,进而将哲水蚤桡足类的安氏伪镖水蚤在饲喂上述两种硅藻条件中连续培养了四个世代,对其每个世代的MnSOD基因表达水平进行了测定与分析。结果显示:牟氏角毛藻饲喂的安氏伪镖水蚤第四个世代Mn SOD基因表达水平显著高于第一个世代,而小新月菱形藻饲喂的安氏伪镖水蚤后三个世代的MnSOD基因表达水平均显著高于第一个世代,推测安氏伪镖水蚤可以对两种硅藻产生遗传适应,并且适应机制可能不同

Apoptosis induced by a novel intestinal metabolite of ginseng saponin in human hepatocellular carcinoma BEL-7402 cells

目的探讨一种新的人参稀有皂苷20-O-(β-D-吡喃葡萄糖)-20(S)-原人参二醇皂苷(IH-901)对人肝癌BEL-7402细胞的增殖抑制效果和诱导凋亡作用。方法以0、6.25、12.5、25、50、75、100μmol/L的IH-901作用于体外培养的BEL-7402细胞,通过MTT法检测IH-901对细胞生长的抑制作用,相差显微镜观察细胞形态变化,AO/EB的荧光核染色观察凋亡细胞形态,流式细胞术检测细胞周期和细胞凋亡。结果MTT实验显示IH-901抑制BEL-7402细胞的生长,呈时间及浓度依赖的方式;显微镜下观察到典型的凋亡形态变化和生化特征:细胞固缩,核染色质凝聚,出现凋亡小体;AO/EB的荧光核染色后,观察到典型的凋亡细胞;流式细胞仪可以检测到凋亡峰,且细胞被阻滞在G0/G1期。结论IH-901能通过诱导细胞凋亡的方式有效地抑制人肝癌BEL-7402细胞的生长,稀有人参皂苷IH-901可能成为一种潜在的抗肝癌药物。Objective To investigate the effects of 20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol(IH-901) on the human hepatocellular carcinma BEL-7402 cells in terms of inhibition of proliferation and induction of apoptosis.Methods BEL-7402 Cells in cultural medium in vitro were given 0,6.25,12.5,25,50,75,and 100 μmol/L IH-901.The inhibitory rate of cells was measured by MTT assay,morphology of cell apoptosis was observed by AO/EB fluorescent staining,cell apoptotic rate and cell phase were detected by flow Cytometry(FCM).Results The results indicated that IH-901 could inhibit the proliferation of BEL-7402 cells significantly.The suppression was both in a time-and dose-dependent manner.Morphological examination of IH-901-treated samples showed cells with chromatin condensation,cell shrinkage,and all typical characteristics of apoptotic cells.Marked morphological changes of cell apoptosis was observed very clearly by AO/EB fluorescent staining.The increase in the apoptotic sub-G1 fraction in a concentration-dependent manner and cell cycle arrest at the G0/G1 phase were detected by FCM.Conclusion The results demonstrate that IH-901 dramatically suppresses BEL-7402 cell growth by inducing programed cell death.These results may provide a pivotal mechanism for the use of IH-901 in the prevention and treatment of human hepatocellular carcinoma BEL-7402 cells,the antiproliferation,and the apoptosis厦门市科技创新基金重点项目(3502Z20062008

The primary investigation of the value of whole body diffusion weighted imaging in diagnosis of metastatic tumor

背景与目的:随着临床上晚期肿瘤病例的增多,为了解治疗前、后全身转移瘤的大小及数量变化的实际情况,以往多采用分部位进行CT和(或)Mr扫描,少部分有条件的患者采用PET检查,但这些方法可能存在过多地暴露于射线、检查时间过长及经济负担过重等问题。因此,我们探讨磁共振的全身弥散加权成像(WHOlE bOdy dIffuSIOn WEIgHTEd IMAgIng,WbdWI)方法对肿瘤及转移灶的检出准确性及其临床应用的可行性。方法:对68例临床已发现原发肿瘤及多个转移灶或发现多个肿瘤病灶,但原发灶不明确的患者进行WbdWI检查,其中男性49例;女性19例。平均年龄61岁。68例均与CT和(或)MrI的检查结果进行比较,其中17例与PET的检查结果相比较。对病灶的检出率,以病灶长径大小分为:<1CM,≥1~<2CM,≥2~<3CM及≥3CM以上4组进行计数分析,同时,对不同部位间的病灶检出情况进行分析。结果:WbdWI对4组病灶的检出率分别为30%、78%、96%及100%。在各个部位的敏感性和准确性中以骨骼最高。对位于肺部小于1CM的病灶和位于颈部、盆腔小于2CM的淋巴结的诊断存在一定困难。结论:WbdWI对大范围的肿瘤病灶筛查是安全、简便、有效和经济的检查新方法。随着技术参数的进一步完善,WbdWI在发现和诊断全身多发肿瘤方面,具有良好的发展潜力。因此,本技术有望作为一种新的、效果好于PET的全身检查技术。Background and purpose:With the increased number of advanced tumors,it is important to evaluate the actual pre-and post-treatment changes in metastatic diseases,such as tumor size and tumor quantity.Previously,CT and/or MRI scan were usually applied,only a few patients could pay for PET(positron emition tomography).But there were several problems we should face,including redundantly exposure to radiation,too long verification time and heavy financial burden.Therefore we evaluated the application of whole body diffusion weighted imaging in diagnosing of tumor and metastatic tumor.Methods:sixty-eight patients(49 male,19 female,age from 29 to 84 years with mean age of 61 years) with a variety of tumors were investigated by combined CT and/or MRI scan,seventeen patients were compared with positron emition tomograph(PET).All tumors were classified into four groups,according to longest diameter of <1 cm,≥1-<2 cm,≥2-<3 cm and ≥3 cm.At the same time,the positive detection rates between different parts of the lesions were analyzed.Results:The detection rate of the four groups were 30%,78%,96% and 100%,respectively.The skeletal system had the highest sensitivity and accuracy in every part of body.There was difficulty in diagnosing lung cancer with longest diameter less than 1 cm as well as tumors located in neck and pelvic with longest diameter less than 2 cm.Conclusion:Whole body diffusion weighted imaging is secure,convenient,effective and economic for screening wide-ranging tumor focus.After improvement of parameters,WBDWI could be as a new effective whole body examination technique.厦门市科技计划项目(No:3502Z20074017