Study of Interaction between Protein and Chitosan by Total Internal Reflection Fluorescence Spectroscopy

壳聚糖作为重要的天然聚合物，以良好的生物相容性、生物可降解性，对细胞组织不产生毒性影响，在生物医学等方面得到了广泛的研究应用。壳聚糖对蛋白质有良好的亲和能力，研究蛋白质与壳聚糖在界面上的相互作用、理解蛋白质与壳聚糖分子在界面接触时的吸附过程变化对蛋白药物的开发和生物化学研究具有非常重要的意义。全内反射荧光法是研究界面蛋白质吸附的一种有效的手段。它利用指数衰减的倏逝波选择性激发介质表面的荧光团分子，产生全内反射荧光，具有高度的界面(表面)特异性，从而可有效排除本体干扰，获取界面信息，而且又具有非破坏性，能够实时、原位检测蛋白质吸附过程。本文致力于改进实验室已有的固/液界面全内反射荧光分析的部件，...As an important natural polymer, chitosan has been applied extensively in biomedicine for its biocompatibility, biodegradation, as well as non-toxicity to cellular tissues. There is good affinity between chitosan and protein. For the development of protein drugs and the research of biochemistry, it is significant to probe the interaction between chitosan and protein, and to observe changes of adso...学位：理学硕士院系专业：化学化工学院化学系_分析化学学号：20042501

Screening of Dihydroxyacetone-producing bacteria from Marine and Optimization of its Fermentation Conditions

二羟基丙酮是一种重要的化工原料，广泛参与各种聚合、缩合反应，工业上应用于食品、制药、化妆品等领域。近年来由于生物柴油的产业过热造成废甘油的大量剩余，使二羟基丙酮（DHA）成为研究热点。本文从厦门海域红树林土壤中分离筛选得到20株可将甘油转化为DHA的菌株，其中ch20-B1转化率较高。对该菌株进行生理生化鉴定实验和16SrRNA基因分析表明，菌株为黄杆菌属(Flavobacterium)的嗜盐黄杆Flavobacteriumhalmsphilum。DHA可以用生长细胞发酵生成，也可以用全细胞静息转化甘油产生。 本文首先确定了嗜盐黄杆Flavobacteriumhalmsphilum生长细胞的...1,3-Dihydroxyacetone (DHA) is a very important compound, widely involved in various polymerization and condensation reaction of industrial process, and usually applied for the production of food, pharmacy, cosmetics and so on. Nowadays, it also has become a research focus due to rapidly increasing surplus of glycerol obtained as a by-product of biodiesel fuel manufactur...学位：工学硕士院系专业：化学化工学院化学工程与生物工程系_生物化工学号：2062008115160

基于语言模型及循环卷积神经网络的事件检测

目前,事件检测的难点在于一词多义和多事件句的检测.为了解决这些问题,提出了一个新的基于语言模型的带注意力机制的循环卷积神经网络模型(recurrent and convolutional neural network with attention based ..

Performance of the fuel injector in supersonic combustor

Enhancing the fuel-air mixing is critical for scramjet combustor performance. The performance of four different aerodynamic ramp injectors was reported in this paper. The experiments were conducted in a direct-connected scramjet test facility. The concentration profiles were obtained by gas sampling and chromatogram analysis. The pictures of the flow field were obtained by using laser scatter. It would be used to analyze the flow field generated by the aerodynamic ramp, then optimize the layout of the injectors. The results would offer some useful information for engine design. Copyright © 2009 by the American Institute of Aeronautics and Astronautics, Inc

Loop-Mediated Isothermal Amplification(LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods

目的:利用环介导等温扩增技术建立食品中酸土环脂芽孢杆菌快速检测方法。方法:针对酸土环脂芽孢杆菌16S序列设计特异引物,再优选反应体系,用显色法检测实验结果。结果:该方法能够在63℃条件下1 H内检出食品中酸土环脂芽孢杆菌,所设计的引物有良好的特异性;灵敏度达6.7 Cfu/M l(弱阳性)。结论:该方法具有高效、特异性强和敏感性高等特点,可满足酸土环脂芽孢杆菌快速检测筛选的要求。Purpose: A loop-mediated isothermal amplification(LAMP) method was established for the detection of Alicyclobacillus acidoterrestris in foods.Methods: After optimization of the reaction conditions of LAMP including the concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity and specificity were evaluated.Results: The method was capable of rapidly and specifically detecting A.acidoterrestris in foods within 1 hour at a constant temperature of 63 ℃.The sensitivity of the method was 6.7 CFU/m L and the specificity was 100%.Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of A.acidoterrestris in various food samples.福建省漳州市自然科学基金项目(ZZ2012J16

The Design of Fire Detection Smart Car Based on CCD

本文以建筑环境检测为研究对象,设计了以STC12C5A60S2单片机为系统控制处理器的对建筑内环境进行自主循环检测的智能模型车,研究了传感器、探测器在建筑环境检测中的应用,实现了智能车的自主调速、避障的功能以及建筑环境的火灾情况检测、显示、报警,工作人员通过CCD传感器采集到的视频图像实时监测检测情况并进行智能车自动/手动选择以及相应情况的处理

论政务信息公开背景下公务人员人事档案信息的披露

政务信息公开是政府工作透明化的必然要求,在政务信息公开中披露与公务活动有关的公务人员人事档案信息对于完善政府信用,满足社会公众的知情权等都有很大的益处。在披露与公务活动有关的公务人员人事档案信息时,应以公共利益为先,限定公开的范围,正确处理好个人信用与政府信用之间的关系

Design of Wireless Dual-axis Tilt Sensor Based on CC1110

无线双轴倾角传感器是利用MEMS倾角传感器采集信号,并通过无线方式传输测量现场中被测物端面倾斜角度的测量仪器。本文介绍了一种以STC89C52单片机为控制核心,结合ADXL202加速度传感器,以及无线芯片CC1110设计的无线双轴倾角传感器。该系统体积小、稳定性好、功耗低、通信效率高,因此可广泛应用于测量、监测等工业领域

Research and Realization on Management of Technique and Quality Control System in Primary Processing Line of Cigarette Factory

论文结合烟草企业制造执行系统的实施,通过其中制丝线工艺与质量控制管理系统模块的研究和实现,提高企业生产和管理效率。通过对烟草行业制丝线的生产特点进行分析与归纳,研究了制丝线的工艺和质量控制关键方法,并针对烟草企业的应用,设计和实现了工艺与质量控制系统,将其作为烟草企业制造执行系统的一部分,通过实际应用验证了该系统的可行性和可靠性,为企业制造执行系统的实施,以及生产管理效率的提高提供了帮助,而且具有实际应用意义和一定的前瞻性

高速DWDM通信技术及进展

综述了高速DWDM（密集波分复用）系统中的关键技术,包括QPSK（四相相移键控）、8QAM（8进制正交幅度调制）和16QAM（16进制正交幅度调制）等新型调制格式、超低损耗光纤/拉曼放大和新型光集成器件,并分析、比较了这些关键技术对DWDM系统传输产生的不同影响。在此基础上,介绍了近年来DWDM大容量传输的一些成功试验。试验表明,这些关键技术有着广阔的应用前景