Screening of Dihydroxyacetone-producing bacteria from Marine and Optimization of its Fermentation Conditions

Abstract

二羟基丙酮是一种重要的化工原料,广泛参与各种聚合、缩合反应,工业上应用于食品、制药、化妆品等领域。近年来由于生物柴油的产业过热造成废甘油的大量剩余,使二羟基丙酮(DHA)成为研究热点。本文从厦门海域红树林土壤中分离筛选得到20株可将甘油转化为DHA的菌株,其中ch20-B1转化率较高。对该菌株进行生理生化鉴定实验和16SrRNA基因分析表明,菌株为黄杆菌属(Flavobacterium)的嗜盐黄杆Flavobacteriumhalmsphilum。DHA可以用生长细胞发酵生成,也可以用全细胞静息转化甘油产生。 本文首先确定了嗜盐黄杆Flavobacteriumhalmsphilum生长细胞的...1,3-Dihydroxyacetone (DHA) is a very important compound, widely involved in various polymerization and condensation reaction of industrial process, and usually applied for the production of food, pharmacy, cosmetics and so on. Nowadays, it also has become a research focus due to rapidly increasing surplus of glycerol obtained as a by-product of biodiesel fuel manufactur...学位:工学硕士院系专业:化学化工学院化学工程与生物工程系_生物化工学号:2062008115160

    Similar works