Study of Interaction between Protein and Chitosan by Total Internal Reflection Fluorescence Spectroscopy

Abstract

壳聚糖作为重要的天然聚合物,以良好的生物相容性、生物可降解性,对细胞组织不产生毒性影响,在生物医学等方面得到了广泛的研究应用。壳聚糖对蛋白质有良好的亲和能力,研究蛋白质与壳聚糖在界面上的相互作用、理解蛋白质与壳聚糖分子在界面接触时的吸附过程变化对蛋白药物的开发和生物化学研究具有非常重要的意义。全内反射荧光法是研究界面蛋白质吸附的一种有效的手段。它利用指数衰减的倏逝波选择性激发介质表面的荧光团分子,产生全内反射荧光,具有高度的界面(表面)特异性,从而可有效排除本体干扰,获取界面信息,而且又具有非破坏性,能够实时、原位检测蛋白质吸附过程。本文致力于改进实验室已有的固/液界面全内反射荧光分析的部件,...As an important natural polymer, chitosan has been applied extensively in biomedicine for its biocompatibility, biodegradation, as well as non-toxicity to cellular tissues. There is good affinity between chitosan and protein. For the development of protein drugs and the research of biochemistry, it is significant to probe the interaction between chitosan and protein, and to observe changes of adso...学位:理学硕士院系专业:化学化工学院化学系_分析化学学号:20042501

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