The construction of multifunctional nanoparticles and anti-glioblastoma research

脑胶质瘤是最常见的中枢神经系统恶性肿瘤，由于其呈恶性浸润性生长，因此传统的治疗方式难以达到良好的治疗效果。光热治疗、光动力治疗、基因治疗等新型肿瘤治疗方式正逐步被应用到抗脑胶质瘤的研究中。但是单一的治疗方式始终难以实现彻底的治疗，因此，联合治疗成为脑胶质瘤治疗的新策略。 本课题构建了两种联合治疗的纳米粒递送系统，以期能够达到更好的脑胶质瘤治疗效果。首先以金纳米棒为核心，包覆二氧化硅，外侧连接近红外染料IR795，构建了AuNRs@SiO2-IR795被动靶向纳米粒子，实现光热和光动力的联合治疗。进一步采用阳离子脂质体包载金纳米棒、阿霉素和YAP-siRNA，以ANG2为靶标进行修饰，旨在构建...Glioma is the most common malignant tumors of the central nervous system. Due to its malignant invasive growth, it was different to achieve the better therapeutic effect. Nowadays, photothermal therapy, photodynamic therapy, gene therapy, et.al, was gradually being applied to antiglioma. But none of the single treatment could completely satisfy the clinical treatment. Therefore, the multimodal the...学位：医学硕士院系专业：药学院_药剂学学号：3232014115342

A Research on the Effect about Internet Wealth Investment Products Such As Yu’ebao to the Personal Business of F Rural Commercial Bank

2013年被称为中国的“互联网金融”元年，先不论这个称呼是否准确，但是2013年确实对于国内“互联网金融”而言，具有十分重大的意义，而这其中的关键就是以“余额宝”为代表的互联网理财产品的横空出世。 “余额宝”为代表的互联网理财产品的诞生，对国内的金融市场产生了巨大的影响，可以说是极具震撼效果，并引发了全社会的高度关注。余额宝等互联网理财产品凭借其良好的流动性和高收益，吸引了大量个人客户，并对银行传统的经营方式造成了重大冲击，分流了部分银行存款。本文选择余额宝作为此类产品的典型代表，对其业务流程和相关特点进行分析，并探讨其成功原因和可能存在的问题。 由于余额宝等互联网理财产品的出现，国内金融...The year 2013 is regarded by some people as the first year of the era of Online Financing marked by the creation of internet wealth investment products. Internet wealth investment products ,represented by Yu'ebao, which offers consumers a way to invest their idle money in money market funds with higher returns than bank deposits, has great impact on domestic financial market and draws dramatic att...学位：管理学硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：1792011115083

Thinkng about Urban cultural individuality

本文立足于当前城市文化发展的背景，以城市与人的角度切入现代化进程中城市文化个性的研究，指出“以人为本”是实现城市文化深层的现代化，保持城市文化个性的根本。Based on the present situation, from the angles of urban and human being, this article discusses the problem of urban cultural individuality, then points out that human being is the essence of cultural modernization and maintaining cultural individuality

实现“建筑、城市、园林绿地的再统一”——厦门市自然开放空间系统的研究

【中文文摘】本文在研究厦门城市规划和得天独厚的自然景观资源的基础上 ,探索具有城市特色的自然开放空间系统的可持续发展

The preparation and characterization of nano-Ag/HAp bioceramic coating on titanium surface

目的:在纯钛表面构筑羟基磷灰石/纳米银涂层并进行各种性能表征。方法:采用恒电位电化学法在纯钛表面构建纳米银/羟基磷灰石(nAnO-Ag/HAP)涂层。采用扫描电子显微镜(SEM),X射线衍射仪(Xrd)、等检测手段对涂层进行理化性能的表征。考察了涂层的结构组分和表面的微观形貌,而后将l-929细胞培养于涂层表面,采用细胞毒性评价,分析比较涂层表面对l-929细胞黏附率及增殖率的影响。结果:涂层纳米银粒子直径在200~400nM时体外细胞培养显示其具有良好的生物活性。结论:本实验构筑的涂层具有良好的性能和应用前景。Objective:This research was aimed at the electrochemical deposition employed to deposit a Nano-Ag/HAp composite coating on titanium surface.Methods:A Nano-Ag/Hap composite coating was deposited on the surface of pure titanium by electrochemical deposition.Scanning electromicroscope(SEM) and X-ray diffraction(XRD) were employed to characterize the obtained coating.In vitro celluar responses of the L-929 to the coating were also evaluated by MTT assay.Conventional Hap coatings and commercially pure titanium were taken as control.Results:The Nano-Ag/Hap bioceramic coating has good crystallization and homogeneous on nano-Scale surface morphology.The L-929 culture exhibits satisfactory bioactivity.Conclusion:The Nano-Ag/HAp bioceramic coating on titanium surface has promising prospects in the future clinical application

The preparation and biological evalution of Hydroxyapatite/Nanosilver bioceramic coating on Titanium implant surface

目的:在钛种植体表面构筑羟基磷灰石/纳米银涂层并进行生物学评价。方法:采用恒电位电化学法在纯钛表面构建纳米级羟基磷灰石/纳米银(HAP/nAnOSIlVEr)涂层。采用扫描电镜(SEM),检测手段对涂层进行理化性能的表征。考察了涂层的结构组分和表面的微观形貌,而后将人牙周膜成纤维样细胞培养于涂层表面,采用细胞毒性评价及其形态观察,分析比较不同表面涂层对牙周膜成纤维样细胞粘附率及增殖率的影响。结果:羟基磷灰石纳米银涂层体外细胞培养显示其具有良好的生物活性。结论:本实验构筑的涂层具有良好的生物相容性和细胞粘附性,应用前景广阔。Objective:To prepare the composite of Hydroxyapatite/Nanosiliver composite coating on titanium implant surface.Method:A HAp/Nanosiliver composite coating was deposited on the surface of pure titanium by electrochemical deposition.Scanning electromicroscope(SEM)was employed to characterize the obtained coating.The reactions of human pe- riodontal ligament fibroblast-like cells (PDLF)to the coating were also evaluated by MTT assay and SEM observation.Re- sult:The HAp/Nanosiliver bioceramic coating has good crystallization and homogeneousness on nano-scale surface mor- phology.The PDLF growth was tested by MTT assay and the cell relative growth rate (RGR)was calculated.Conclusion: The HAp/Nanosiliver bioceramic coating on titanium surface possesses good biocompatibility and celluar adhesion and has promising prospects in the future clinical application.厦门市科技局资助项目(3502Z20084034

小鼠端粒酶蛋白亚单位重组腺病毒载体的构建及鉴定

【目的】构建小鼠端粒酶蛋白亚单位（mouse telomerase reverse transcriptase，mTERT）基因重组腺病毒载体，为下一步研究其体外表达和动物实验研究提供基础。【方法】从肝癌细胞中提取总RNA。采用RT-PER技术扩增mTERT的基因编码区序列，将序列定向克隆至真核表达载体pAC，将此表达质粒与腺病毒重组质粒pJM17共同转染293细胞，经同源重组产生重组腺病毒载体Ad-mTERT，纯化后的重组腺病毒载体Ad-mTERT在293细胞大量扩增并通过氯化铯密度梯度离心法纯化，测定病毒滴度。【结果】PCR及酶切证实：mTERTDNA正确克隆到穿梭质粒pAC中，带mTERTDNA的表达盒成功重组到腺病毒载体基因组E1A缺失区，并在293细胞中成功包装出具有感染活性的重组腺病毒Ad-mTERT。【结论】成功构建了小鼠TERT基因重组腺病毒载体，为研究其用于肿瘤的基因治疗奠定基础

原生态文化与中国传统

第九届人类学高级论坛于2010年6月23～25日在贵州省凯里学院举行。会议期间,《光明日报》国学版主编梁枢先生组织了一个关于"原生态文化与中国传统"的小型座谈会,会议由广西民族大学徐杰舜教授主持,参加人员有中国人民大学郑杭生教授、中国社会科学院叶舒宪研究员、四川大学徐新建教授、厦门大学彭兆荣教授、中国人民大学陆益龙副教授、浙江师范大学林敏霞博士等。座谈会就原生态文化与中国传统文化尤其是国学的关系展开了深入探讨