The preparation and biological evalution of Hydroxyapatite/Nanosilver bioceramic coating on Titanium implant surface

目的:在钛种植体表面构筑羟基磷灰石/纳米银涂层并进行生物学评价。方法:采用恒电位电化学法在纯钛表面构建纳米级羟基磷灰石/纳米银(HAP/nAnOSIlVEr)涂层。采用扫描电镜(SEM),检测手段对涂层进行理化性能的表征。考察了涂层的结构组分和表面的微观形貌,而后将人牙周膜成纤维样细胞培养于涂层表面,采用细胞毒性评价及其形态观察,分析比较不同表面涂层对牙周膜成纤维样细胞粘附率及增殖率的影响。结果:羟基磷灰石纳米银涂层体外细胞培养显示其具有良好的生物活性。结论:本实验构筑的涂层具有良好的生物相容性和细胞粘附性,应用前景广阔。Objective:To prepare the composite of Hydroxyapatite/Nanosiliver composite coating on titanium implant surface.Method:A HAp/Nanosiliver composite coating was deposited on the surface of pure titanium by electrochemical deposition.Scanning electromicroscope(SEM)was employed to characterize the obtained coating.The reactions of human pe- riodontal ligament fibroblast-like cells (PDLF)to the coating were also evaluated by MTT assay and SEM observation.Re- sult:The HAp/Nanosiliver bioceramic coating has good crystallization and homogeneousness on nano-scale surface mor- phology.The PDLF growth was tested by MTT assay and the cell relative growth rate (RGR)was calculated.Conclusion: The HAp/Nanosiliver bioceramic coating on titanium surface possesses good biocompatibility and celluar adhesion and has promising prospects in the future clinical application.厦门市科技局资助项目(3502Z20084034

The preparation and characterization of nano-Ag/HAp bioceramic coating on titanium surface

目的:在纯钛表面构筑羟基磷灰石/纳米银涂层并进行各种性能表征。方法:采用恒电位电化学法在纯钛表面构建纳米银/羟基磷灰石(nAnO-Ag/HAP)涂层。采用扫描电子显微镜(SEM),X射线衍射仪(Xrd)、等检测手段对涂层进行理化性能的表征。考察了涂层的结构组分和表面的微观形貌,而后将l-929细胞培养于涂层表面,采用细胞毒性评价,分析比较涂层表面对l-929细胞黏附率及增殖率的影响。结果:涂层纳米银粒子直径在200~400nM时体外细胞培养显示其具有良好的生物活性。结论:本实验构筑的涂层具有良好的性能和应用前景。Objective:This research was aimed at the electrochemical deposition employed to deposit a Nano-Ag/HAp composite coating on titanium surface.Methods:A Nano-Ag/Hap composite coating was deposited on the surface of pure titanium by electrochemical deposition.Scanning electromicroscope(SEM) and X-ray diffraction(XRD) were employed to characterize the obtained coating.In vitro celluar responses of the L-929 to the coating were also evaluated by MTT assay.Conventional Hap coatings and commercially pure titanium were taken as control.Results:The Nano-Ag/Hap bioceramic coating has good crystallization and homogeneous on nano-Scale surface morphology.The L-929 culture exhibits satisfactory bioactivity.Conclusion:The Nano-Ag/HAp bioceramic coating on titanium surface has promising prospects in the future clinical application

Study on bio-safety for Nano-Ag/HAp as anti-bacterial materials

通信作者:史建陆Tel:(0576)85199572 E-mail: zmfjmu@ gmail．com[中文文摘]目的研究纳米银羟基磷灰石的细胞毒性。方法采用能快速、准确、敏感地评价细胞增殖率和细胞毒性的MTT比色法,以纳米羟基磷灰石为对照组,纳米银羟基磷灰石为实验组,在材料浸提液中,分别检测人牙周膜成纤维样细胞的相对增殖率。另观察细胞在扫描电镜中的形态,从而分析比较涂层表面对细胞的毒性。结果与纳米羟基磷灰石相比,纳米银羟基磷灰石涂层中的纳米银粒子直径在200～400nm时体外细胞培养显示表现出较高的细胞增殖率,细胞毒性趋于0级,显示其具有良好的生物活性。结论纳米银羟基磷灰石涂层无细胞毒性。[英文文摘]Objective To study the cytotoxicity of a Nano-Ag/HAp composite coating on titanium surface.Methods A Nano-Ag/HAp composite coating was deposited on the surface of pure titanium by electrochemical deposition.In vitro celluar responses of the PDLF to the coating were also evaluated by MTT assay.Conventional HAp coatings and commercially pure titanium were taken as control.Results The Nano-Ag/Hap bioceramic coating has good crystallization and homogeneity on nano-scale surface morphology.The PDLF culture exhibits satisfactory bioactivity．Conclusions The Nano-Ag /HAp bioceramic coating on titanium surface has promising prospects in the future clinical application

The Effect of TanshinoneIIA on the Morphology and Terminal Differentiation of MG-63 Cell

目的:观察中药有效成分丹参酮IIA(Tan IIA)对人成骨肉瘤MG-63细胞形态与超微结构和相关终末分化指标的影响,以鉴定其对肿瘤细胞终末分化的诱导作用。方法:0.5μg/mL丹参酮IIA处理MG-63细胞,光镜、电镜观察和免疫细胞化学检测系统研究MG-63细胞处理前后细胞形态、超微结构变化和成骨细胞相关终末分化蛋白的表达变化。结果:光镜与电镜观察结果显示经Tan IIA处理细胞产生了核质比例减小、异染色质减少、常染色质增多、细胞器丰富发达、细胞表面微绒毛减少等显著变化;免疫细胞化学检测显示处理后MG-63细胞Ⅰ型胶原蛋白、骨粘素和骨钙蛋白的表达呈阳性,并观察到钙化糖原颗粒增多和典型骨结节的形成。结论:丹参酮IIA能显著改变MG-63细胞形态与超微结构恶性特征,并促进与成骨细胞相关的终末分化指标的表达变化,从而对人成骨肉瘤细胞的终末分化具有明显的诱导作用。Objective:TanshinoneⅡA (TanⅡA)is effective anticancer and antioxidant constituent of traditional Chinese herbal medicine Danshen(Salvia mitiorrhiza).The purpose of this study is to investigate the biological effect oftanshinoneⅡA on the cell mor- phology and terminal differentiation of the human osteosarcoma MG-63 cells.Methods:MG-63 cells treated with or without 0.5μg/mL tanshinoneⅡA were subjected to immuncocytochemical assay,light and electro-microscopy.The cells treated with hexamethylene bisac- etamide were also investigated as positive control of induced differentiation.Results:Immunocytochemistry analysis revealed that the ex- pression of the molecular biomarkers of terminal differentiation,including osteocalcin,osteonectin and calcified glycogen particles,were strongly positive.The classic bone nodules were observed in the tanshinoneⅡA treated cells.Light and electro-microscopy observation analysis revealed that the configuration and ultrastructure of MG-63 had undergone restorational changes similar to that of normal cells after tanshinoneⅡA treatments.Conclusion:TanshinoneⅡA could reverse the malignant phenotypes of MG-63 cells,promote the expres- sion of terminal differentiational biomarkers,and induce cell into terminal differentiation.国家自然科学基金(No．30470877);; 国家基础研究(973计划，No．2007CB935603)资助项

The Effects of Ginsenoside Rg1 on the Morphology and Terminal Differentiation of Human Osteosarcoma MG-63 Cell

为了研究人参皂甙rg1对人成骨肉瘤Mg-63细胞(以下简称“Mg-63细胞“)形态与超微结构及终末分化指标的影响,鉴定其对Mg-63细胞的诱导分化作用,以50μg/Ml人参皂甙rg1处理Mg-63细胞,光学显微镜与电子显微镜观察Mg-63细胞形态、超微结构变化,免疫细胞化学方法检测成骨细胞相关终末分化蛋白的表达变化,并同步以HMbA处理Mg-63细胞作为阳性对照.光学显微镜与电子显微镜观察结果显示细胞形态与超微结构产生了细胞形态规则、大小一致、细胞铺展体积增大,核质比例减小、核内核仁数目减少、细胞器丰富发达等与正常细胞相似的恢复性变化.观察到Mg-63细胞终末分化指标I型胶原、骨粘素、骨钙蛋白的阳性表达及钙化糖原颗粒的增多与典型骨节结的形成,其变化结果与HMbA处理细胞类似.本研究证实人参皂甙rg1能显著改变Mg-63细胞形态与超微结构恶性特征,并增强成骨细胞相关的终末分化指标的表达,从而对Mg-63细胞的终末分化具有一定的诱导作用.To investigate the biological effects of ginsenoside Rg1,the human osteosarcoma MG-63 cells,which were treated with 50 μg/mL ginsenoside Rg1,were subjected to immuncocytochemical assay,light and electro-microscopy.The cells treated with hexamethylene bisacetamide were also investigated as positive control of induced differentiation.It was found by light and electro-microscopy that the configuration and ultrastructure of MG-63 had undergone restorational changes similar to that of normal cells after ginsenoside Rg1 treatments.The observed results are as follows: the morphology of the cells were regular and inclined to the same volume.The cells tended to be flat and spread,the size increased,the nucleo-cytoplasm ratio lessened,the number and size of nucleous decreased,the organelle were well-developed,typical and polarized.Immunocytochemistry analysis revealed that the expression of the molecular biomarkers of terminal differentiation such as osteocalcin,osteonectin and calcified glycogen particles were strongly upregulated compared with the control groups.The calcified hepatin granules increased and the tybical bone nodules were obsreved in the ginsenoside Rg1 treated cells.From these we can confirm that cinnamic acid could reverse the malignant phenotypes of MG-63 cells,promote the expression of terminal differentiational biomarkers,and induce cell into terminal differentiation.国家自然科学基金(30470877);国家基础研究(973计划;2007CB935603);福建省自然科学基金(2008J0302);中国博士后科学基金(20070420754)资

锂离子在石墨烯材料中的嵌入脱出机制

采用还原氧化石墨法制备了石墨烯材料,运用X射线衍射(Xrd)、原子力显微镜(AfM)、扫描电子显微镜(SEM)、充放电、循环伏安和电化学阻抗谱(EIS)等对其结构、表面形貌和电化学嵌锂性能进行了表征.Xrd,SEM和AfM研究结果表明,所制备的材料主要为层数少于10层的石墨烯材料;充放电结果表明,石墨烯材料电极具有较高的可逆容量和较好的循环性能,但也存在较大的首次不可逆容量,不可逆容量主要归因于首次充放电过程中石墨烯材料表面固体电解质相界面膜(SEI膜)的形成和充放电循环过程中石墨烯材料的自发堆叠.EIS结果表明,石墨烯材料电极表面SEI膜主要在0.95--0.7V之间形成,测得锂离子在石墨烯材料电极中电化学嵌入反应的对称因子α为0.446.中央高校基本科研业务费专项资金(2010LKHX03;2010QNB04;2010QNB05);中国矿业大学科技攀登计划(ON090237);国家大学生创新性实验计划资助项