A QUICK METHOD TO MEASURE THE SAMPLE CUR-RENT OF THE FLOW CYTOMETER AND ITS ERROR MARGIN

第一作者: 彭兴跃, 出生于1966 年, 博士, 副教授。E-mail:xypeng @ xmu. edu. cn国家自然科学基金资助项目49806004号;高等学校骨干教师资助计划资助

毛细管区带电泳法测定低卡路里食品中甜菊苷

厦门大学固体表面物理化学国家重点实验室基金资助项

用流式细胞仪研究秋茄叶柄薄壁细胞大小及叶绿素含量与海水盐度的关系

采用快速离析方法和流式细胞仪研究了红树植物秋茄成熟叶柄薄壁细胞的大小和叶绿素含量与海水盐度之间的关系。结果表明 ,海水盐度为 0 .8%～ 2 .0 %时 ,随着盐度的升高 ,叶柄薄壁细胞大小和单位体积叶绿素含量的变化趋势与叶肉细胞一致。海水盐度为 0 .8%～ 1.0 %时 ,随着盐度升高 ,向有利于提高光合作用强度的方向发展 ;海水盐度在 1.0 %～ 2 .0 %之间时 ,随着盐度的升高 ,向加强生理干旱适应性方向发展。海水盐度高于2 .0 %时 ,叶柄薄壁细胞的大小和单位体积薄壁组织叶绿素含量随盐度的变化趋势与叶肉细胞不同 ,原因尚待进一步研究。实验结果表明 ,借助快速离析方法 ,使用流式细胞仪研究器官细胞相关特性是可行的 ,为从形态学角度研究红树植物耐盐机理提供了可能 ,扩大了流式细胞仪的应用领

Analysis of the Content of Stevia Sweeteners by Capillary Zone Electrophoresis

本文介绍了一种用毛细管区带电泳法筛选甜菊糖苷突变体的有效方法。根据实验结果 ,优化的电泳条件为 :60mmol/LTris 硼酸缓冲液 (pH 8.0 ) ,柱温 30℃ ,工作电压 2 5kV。优化条件下 ,甜菊苷 (Ste vioside)迁移时间的R .S .D为 0 .45% (1 5次 ) ,且在 7.45× 1 0 - 5～ 1 .74× 1 0 - 2 mol/L的浓度范围内存在良好的线性关系 (r=0 .9994) ,甜菊主要糖苷在 5min内均可实现分离。在优化条件下 ,本实验研究了低能离子注入后甜菊主要糖苷含量变化 ,结果令人满意。This paper introduced an effect capillary zone electrophoresis method for screening of stevia mutation. According to the experimental results, the optimum separation conditions were selected as: 60 mmol/L Tris tetraborate buffer of pH 8.0, 30 ℃ and 25 KV. Under optimum conditions, the R.S.D. of stevioside migration time for 15 runs was 0.45%. The detector response for stevioside was linear over the range of 7.45×10 -5 ～1.74×10 -2 mol/L (r=0.9994). Stevia main sweeteners cound be separated effectively in less than five minutes. This method was applied for determining the variation of stevia main sweeteners after low_energy ions implantation

Development of bivalent vaccines against foot-and-mouth disease

口蹄疫(Foot-and-mouthdisease，FMD)是由口蹄疫病毒(Foot-and-mouthdiseasevirus，FMDV)引起的烈性传染病。由于猪、牛、羊等主要家畜均可感染此病，并能形成大规模地流行，所以国际兽疫局将该病列为A类家畜传染病之首。FMDV有七种血清型，目前我国以O型FMDV为主，A型FMDV也时有发现。研制抗A、O型FMDV的双价疫苗具有极其重要的意义。本研究主要包括研制大肠杆菌、酵母表达的A、O型FMDV双价多肽疫苗；制备A、O型FMDV双价DNA疫苗及如何提高DNA疫苗的免疫效果等内容。现将具体结果总结如下： 1.大肠杆菌表达的A、O型FMDV双价多肽疫苗...Foot-and-mouth disease (FMD), caused by a picornavirus of the Aphtovirus genus (FMDV), is a highly contagious and economically devastating disease of swine, cattle and other cloven-hoofed animals. FMDV consists of different serotypes. Type O FMDV was the main that caused FMD in China, and type A often broke out. Research on bivalent vaccines against both type A and O FMDV is important. This resear...学位：理学博士院系专业：生命科学学院生物学系_动物学学号：B20012600

Progress in the studies of keratinocyte growth factor

角质细胞生长因子(KGF)从属于成纤维细胞生长因子家族。KGF基因表达受多种细胞因子调控。KGF与受体KGFR特异性的结合发挥其多种生物学功能:参与组织、器官的发育;参与皮肤、胃、肠、肾、膀胱、肺等上皮的损伤修复;减少放、化疗所带来的副作用,具有损伤防护功能;KGF与肿瘤密不可分。Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor family. A lot of cytokinesare responsible for KGF expression. By binding with its receptor KGFR, KGF does its biological functions in repairprocesses of various tissues and organs, including skin, bowel, bladder, kidney, lung and so on. KGF can stimulatedevelopment of some tissues and organs. It also acts as a protective factor on injury induced by radiation andchemotherapy. KGF is concerned with some tumors.福建省自然科学基金资助项目(B0210002

Application of Capillary Electrophoresis to UDPG-Steviaglucoside Glucosyltransferasas Activity Assay

以Tris-硼酸作为毛细管电泳分离分析甜菊糖苷新体系,分离测定UDPG-甜菊糖苷葡萄糖基转移酶在适当条件下催化甜菊醇反应后的甜菊糖苷产物。结果表明,甜菊种子经能量75keV,剂量1014ions/cm2的碳、氮离子注入处理,UDPG-甜菊糖苷葡萄糖基转移酶活性比未进行离子注入的高;而且碳离子注入效果好于氮离子注入。因此,毛细管电泳可以作为离子注入处理植物种子检测的新方法,与文献报道的非Tris-硼酸体系相比,具有性能稳定、缓冲容量大、分离分析速度快、线性范围宽及分辨能力强等优点。Tris-H3BO3 buffer was used as a new capillary zone electrophoresis(CZE) system for separating and determining two main stevia glucosides so-called stevioside (SS) and rebaudioside (RA) produced in the process of the steviol catalyzed by UDPG-steviaglucoside glucosyltransferase properly. By implanting C and N ions with 75keV under the dose of 1014 ions/cm2, it was found that the activity of UDPG-steviaglucoside glucosyltransferases in stevia seeds was higher than that of seeds without ions implantation, and the effect of implanation of C ions was better than that of N ions. Therefore, CZE could provide a novel method for evaluating effects of ions implantation method used for treating plant seeds.厦门大学高等学校重点实验室访问学者基金;; 国家自然科学基金(No.20005005)资助项目

Cloning of Recombinant Keratinocyte Growth Factor and Its Construction of Plant Expression Vector

从鼠肺中提取RNA,设计相应的引物,经RT PCR扩增出角化细胞生长因子cDNA序列;插入pMD18 T载体,经DNA测序证实为KGF基因.将KGF基因经酶切后连接于植物CaMV35S启动子和NOS终止子之间,单酶切插入pBI1301植物双元表达载体,转化大肠杆菌,阳性克隆提取质粒后转化农杆菌EHA105,实现KGF植物表达载体的构建,为KGF的进一步研究奠定基础.Abstract: The cDNA gene encoding keratinocyte growth factor (KGF) was amplified from mouse lung by RT-PCR and inserted into pMD18-T vector. The result of automatic sequence showed that the sequence of inserted fragment was KGF gene. The KGF gene was inserted into pBPFΩ7 under the control of CaMV35S promotor and NOS terminator, then was digested and inserted into pBI 1301 to form the plant binary expression vector. The plant binary expression vector of KGF was transformed into Agrobacterium EHA 105. It provided a reliable basis for the further study on KGF.福建省自然科学基金(B0210002)资助(2002年

Three-dimensional Reconstruction of the Pollen Grain of Salvia splendens Ker-Gawl Using LSCM

应用激光扫描共聚焦显微镜对一串红花粉粒进行连续“光学切片”扫描,利用Laser-Sharp图像处理软件重建其三维立体空间结构。结果显示,重建的图像为高分辨率的三维原色图像,并可沿X、Y、Z轴旋转或任意角度显示。叙述了激光扫描共聚焦显微镜成像原理、特点以及在植物孢粉学研究中的应用前景。Laser scanning confocal microscopy was used to acquire sequential "optical sections" of the pollen of Salvia splendens Ker-Gawl. The three-dimensional structure of the pollen was then reconstructed by means of Laser Sharp software. The result demonstrated that the reconstructed 3-dimensional images were of primary color images with high resolution power, and could be rotated and observed from any angle in the 3D space. The optical principle and characteristics of LSCM with the prospect in applications of LSCM in palynology research were also described in the paper

Application of laser scanning confocal microscopy in FISH

简要介绍激光扫描共聚焦显微镜在荧光原位杂交中的应用方法及其优越性。Brief instruction of application methods of Laser Scanning Confocal Microscoy in FISH and its advantage