亚贡叶的化学成分研究

目的研究新引种的抗糖尿病植物亚贡Smallanthus sonchifolius叶的化学成分。方法应用多种柱色谱方法进行分离和纯化,NMR和MS等波谱解析化合物结构。结果从亚贡叶的乙醇提取物中分离出6个化合物,其结构分别鉴定为:5,8-二羟基-(5H,8H)-β-紫罗兰醇(Ⅰ)、对映-贝壳杉烷-3β,16β,17-三醇(Ⅱ)、对映-贝壳杉烷-16β,17-二醇-19-酸(Ⅲ)、3,4-二羟基苯甲醛(Ⅳ)、正二十五醇(Ⅴ)和正二十八醇(Ⅵ)。结论化合物~均为首次从本植物中分离得到,化合物为首次发现的β-紫罗兰醇型新化合物,命名为亚贡醇(sonchifolol)

Synthesis and characterization of a novel galactosylated cholesterol

A novel galactosylated cholesterol with mono-galactoside moiety, (5-cholesten-3-yl)4-oxo-4-[2-(Iactobionylamido)ethylamido]butanoate 6 was synthesized. Its chemical structure was characterized by IR, ESI-MS, C-13 NMR, H-1 NMR. Doxorubicin entrapped in the liposomes containing 10% mol/mol 6 was rapidly accumulated in liver and mainly uptake by parenchymal cells in mice

Two New Cytotoxic Indole Alkaloids from a Deep-Sea Sediment Derived Metagenomic Clone

National Natural Science Foundation of China [81102333, 81273400]; Fujian Natural Science Foundation for Distinguished Young Scholars [2012J06020]; R&D Special Fund for Public Welfare Industry (Oceanography) [201005022-1, 201005032-1]; China Ocean Mineral Resources RD Association [COMRA DY125-15-T-07]; Xiamen South Marine Center project [13GZP002NF08]; Fundamental Research Funds for the Central Universities [2010121108]Two new indole alkaloids, metagenetriindole A (1) and metagenebiindole A (2), were identified from deep-sea sediment metagenomic clone derived Escherichia coli fermentation broth. The structures of new compounds were elucidated by spectroscopic methods. The two new indole alkaloids demonstrated moderately cytotoxic activity against CNE2, Bel7402 and HT1080 cancer cell lines in vitro

Quantitative Determination of Smaditerpenic Acids A and C in Leaves of Smallanthus sonchifolius by HPLC-MS

通信作者：[email protected][中文文摘]建立了同时测定亚贡(Smallanthus sonchifolius)叶中亚贡二萜酸A和C含量的方法.采用高效液相色谱-质谱法,多反应监测(MRM)模式,大黄素为内标,色谱柱:UltimateTM XB-C18(150mm×4.6mm,5μm);流动相:V(甲醇)∶V(0.5%醋酸水溶液)=90∶10;流速:1.0mL/min;柱温:25℃.亚贡二萜酸A和C的线性范围均为0.2～10.0μg/mL(r≥0.999),平均加样回收率(n=6)分别为96.6%和92.4%,相对标准偏差(RSD)分别为1.9%和3.2%.实验数据表明,该方法操作简单、准确,且重复性好,可用于亚贡叶的质量控制.[英文文摘]A new fast and sensitive high-performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) method was developed and validated for smaditerpenic acids A and C in leaves of Smallanthus sonchifolius using emodin as internal standard(IS).The calibration curves of smaditerpenic acids A and C were investigated with a good linear relationship within the rangs of 0.2-10.0 μg/mL(r≥0.999),and the recoveries(n=6) were 96.6% and 92.4% with RSD of 1.9% and 3.2%,respectively.The analytes were detected in negative ion mode using multiple reaction monitoring(MRM).The method was validated and the specificity,linearity,lower limit of quantitation(LLOQ)precision,accuracy,recoveries and stability were determined.福建省自然科学基金项目(2009J01192);厦门市重大科技计划项目(3502Z20100006

HPLC-MS/MS法同时测定大鼠血浆中3种蟾皮主要活性成分

建立大鼠血浆中3种蟾皮提取物的HPLC-MS/MS测定方法,并用于大鼠体内的药代动力学研究.采用静脉给药和动脉取血的方式,甲醇直接沉淀蛋白的方法快速制备样品;分析柱为Agilent Zorbax Eclipse XDB-C18(150mm×4.6mm,5μm),流动相为乙腈/水(体积比65∶35),流速为1.0mL/min;质谱条件为ESI(+)电离方式,扫描方式为定量的多反应监测(MRM).结果表明,3种提取物分析方法的线性范围均为5～50 000ng/mL(r>0.999),样品的检出限均为5ng/mL,日内和日间精密度的相对标准偏差(RSD)≤4.02%,方法的回收率均在78%以上;经此方法测得的药代动力学数据表明,3种提取物在大鼠体内有相似的药代动力学行为.本方法样品预处理快速简便,检测专一灵敏,适合于3种蟾皮提取物的药代动力学研究

Three novel compounds from the leaves of Smallanthus sonchifolius

Three novel compounds, together with five known ingredients, octacosanol, 3',4',5-trihydroxy-3,7-dimethoxyflavone, 3,4-dihydroxybenzaldehyde, isorhamnetin, and ent-kaurane-3,16,17-triol, were obtained from the leaves of Smallanthus sonchifolius (yacon), and their structures were elucidated as ent-kaurane-3,16,17,18-tertol (1), 3R,7E-9-butoxyl-megastigma-3-ol-3-O--d-glucopyranoside (2), and 3S,5R,6Z-megastigma-6-en-3,5,8,9-tertol (3) on the basis of spectroscopic and chemical methods

Two new compounds from marine-derived fungus Penicillium sp F11

National Natural Science Foundation of China [81072549]; Ministry of Science and Technology of China [2007AA091904]; Natural Science Foundation of Fujian Province of China [2010J05087]; Science and Technology Planning Key Project of Fujian Province, China [2009N0040]Two new compounds, penicillone A (1) and penicillactam (2), were isolated together with 17 known compounds from a marine-derived fungus Penicillium sp. F11. The structures of the new compounds as well as a firstly literatural reported known compound (3) were assigned by spectroscopic methods including 1D/2D NMR and MS analysis techniques. Their cytotoxicities against HT1080, Cne2, and Be17402 cell lines were also evaluated

Constituents with radical scavenging effect from Opuntia dillenii: Structures of new alpha-pyrones and flavonol glycoside

The aqueous ethanolic extract from the fresh stems of Opuntia dillenii HAW. showed potent radical scanvenging activity. Three new compounds, opuntioside I, 4-ethoxyl-6-hydroxymethyl-alpha-pyrone, and kaempferol 7-O-beta-D-glucopyranosyl-(1-->4)-beta-D-glucopyranoside, were isolated from the extract. The structures of the new compounds were determined on the basis of chemical and physicochemical evidence and the radical scavenging effects of principal constituents were examined

Two new alpha-pyrones and other components from the cladodes of Opuntia dillenii

The aqueous ethanolic extract from the fresh cladodes of Opuntia dillenii HAW. was found to show anti-inflammatory activity. Two new alpha-pyrones, named opuntioside II (1) and opuntioside III (2), were isolated from the extract together with six known compounds. The structures of the new compounds were determined on the basis of chemical and physicochemical evidence

Structure elucidation and complete NMR spectral assignments of four new diterpenoids from Smallantus sonchifolius

Four new diterpenoids, named smaditerpenic acid A-D, together with five known compounds, were isolated from the H(2)O extract of the leaves of Smallantus sonchifolius (yacon) cultivated in Liaoning, China and their structures were elucidated on the basis of one- and two-dimensional NMR (including (1)H, (13)C-NMR, (1)H-(1)H COSY, HSOC, TOCSY, HMBC, and ROESY), electrospray ionization mass spectrometry (ESI-MS), and chemical methods. Copyright (C) 2008 John Wiley & Sons, Ltd