通信作者：[email protected][中文文摘]建立了同时测定亚贡(Smallanthus sonchifolius)叶中亚贡二萜酸A和C含量的方法.采用高效液相色谱-质谱法,多反应监测(MRM)模式,大黄素为内标,色谱柱:UltimateTM XB-C18(150mm×4.6mm,5μm);流动相:V(甲醇)∶V(0.5%醋酸水溶液)=90∶10;流速:1.0mL/min;柱温:25℃.亚贡二萜酸A和C的线性范围均为0.2～10.0μg/mL(r≥0.999),平均加样回收率(n=6)分别为96.6%和92.4%,相对标准偏差(RSD)分别为1.9%和3.2%.实验数据表明,该方法操作简单、准确,且重复性好,可用于亚贡叶的质量控制.[英文文摘]A new fast and sensitive high-performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) method was developed and validated for smaditerpenic acids A and C in leaves of Smallanthus sonchifolius using emodin as internal standard(IS).The calibration curves of smaditerpenic acids A and C were investigated with a good linear relationship within the rangs of 0.2-10.0 μg/mL(r≥0.999),and the recoveries(n=6) were 96.6% and 92.4% with RSD of 1.9% and 3.2%,respectively.The analytes were detected in negative ion mode using multiple reaction monitoring(MRM).The method was validated and the specificity,linearity,lower limit of quantitation(LLOQ)precision,accuracy,recoveries and stability were determined.福建省自然科学基金项目(2009J01192);厦门市重大科技计划项目(3502Z20100006
