User-friendliness of the University of Hong Kong for wheelchair-bound persons

Thesis (B.Sc)--University of Hong Kong, 2008.Includes bibliographical references (p. 223-233).published_or_final_versio

ONE NEW SPECIES AND ONE NEW RECORD SPECIES of EIRENIDAE FROM THE TAIW AN STRAIT,CHINA

记述了采自台湾海峡软水母亚纲lEPTOMEduSAE HAECkEl,1866、和平水母科EIrEnIdAE HAECkEl,1879 1新种,无疣杯水母PHIAlOPSIS AVErruCIfOrMIS SP.nOV.和我国海域1新纪录种,母芽侧丝水母HElgICIrrHA MEduSIfErA(bIgElOW,1909),对其形态特征进行描述。模式标本保存于国家海洋局第三海洋研究所。The samples of Eirenidae were collected from the Taiw an Strait,China( 20.85°- 27.06° N,114.92°- 121.24°E) during July 2006 and Jan.2007.One new species of Phialopsis averruciformis sp.nov.and one new record species of Helgicirrha medusifera( Bigelow,1909) are described in the present paper based on the type specimens from the T aiw an Strait,China.The specific diagnosis of the new species is as follows.我国近海海洋综合调查与评价(908专项)(908-ZC-Ⅱ-02;908-01-BC07;908-02-0102;908专项海洋生物样品库); 国家海洋局“全球变化与海气相互作用专项”资助~

拟柄突和平水母在中国的首次发现及其生活史观察

　报道青岛海洋科技馆培养的和平水母属(软水母亚纲, 和平水母科) 一种我国的新纪录———拟柄突和平水母Eirene lacteoides Kubota and Horita , 1992。首次提供该种水母水螅体的形态, 并对各期水母体的特征进行描述, 为编写水螅水母动物志提供参考。国家自然科学基金（40331004，40606033

EFFECTS OF TACHYPLESIN AND N-SODIUM BUTYRATE ON THE MORPHOLOGY AND ULTRASTRUCTURE CHANGES OF HUMAN GASTRIC ADENOCARCINOMA CELLS LINE BGC-823

目的以正丁酸钠为平行对照,比较观察鲎素和癌细胞诱导分化物对人胃癌细胞形态与超微结构变化的影响。方法采用光镜、扫描电镜与透射电镜,观察经2.0 mg/L鲎素、2.0 mmol/L正丁酸钠和1.0 mg/L鲎素+1.0 mmol/L正丁酸钠组合处理的人胃腺癌BGC-823细胞。结果光镜观察显示,鲎素和正丁酸钠以及鲎素+正丁酸钠处理的BGC-823细胞形态均较为一致,细胞体积增大、趋于扁平铺展状态,细胞核质比例减小,细胞核形状较圆整,核仁数量减少。扫描与透射电镜观察可见经鲎素、正丁酸钠及其组合处理的BGC-823细胞均出现细胞表面微绒毛稀少,细胞边缘丝状伪足减少,片状伪足增多;细胞核形态较规则,核内异染色质减少,常染色质增多:细胞质内线粒体增多,结构较为一致,高尔基复合体呈较为典型发达状态,并出现粗面内质网增多和多聚核糖体减少等变化。结论鲎素具有与正丁酸钠等诱导分化物相似的改变人胃癌细胞形态与超微结构恶性表型特征的作用,和与诱导分化物协同加成的诱导分化效果。Objective To compare the morphology and ultrastructure changes of human gastric adenocarcinoma cell line treated by tachyplesin and n-sodium butyrate. Methods Light microscope,scanning electron microscope and transmission electron microscope were used to observe human gastric adenocarcinoma cell line BGC-823 treated by 2.0?mg/L tachyplesin,2.0?mmol/L n-sodium butyrate and 1.0?mg/L tachyplesin in combination with 1.0?mmol/L n-sodium butyrate respectively. Results Light microscope observation showed that BGC-823 cells treated by tachyplesin,n-sodium butyrate and tachyplesin+n-sodium butyrate possessed the similar cell modality as follow: the volume of cell increased,the shape of cell became flat and outspread,nucleo-cytoplasmic ratio decreased,the shape of nucleus was rounde,the number of nucleolus decreased.Scanning electron microscope and transmission electron microscope observation showed that,in the BGC-823 cells which were treated with tachyplesin,n-sodium butyrate and tachyplesin in combination with n-sodium butyrate respectively,microvilli and filopodia reduced,sheed pseudopodia increased,the shape of nucleus became regular,heterochromatin decreased while euchromatin increased,the number of mitochondria increased and its structure appeared consistent,Golgi complex turned to be typical,the amount of rough endoplasmic reticulum increased and that of polyribosome decreased.Conclusion All of these results showed that tachyplesin possessed the similar effects to n-sodium butyrate on changing morphology and ultrastructure of human gastric adenocarcinoma cells and possessed an additive role of inducing tumor cells to differentiate cooperatively with n-sodium butyrate to induce carcinoma cell differentiation.国家自然科学基金资助[30170724

DESCRIPTIONS of ONE NEW GENUS AND TWO NEW SPECIES of HYDROIDOMEDUSAE FROM TAIWAN STRAIT

记述台湾海峡生物综合调查区水螅水母纲1新属2新种,即花水母亚纲面具水母科台湾似内胞水母PArAEuPHySIllA TAIWAnEnSIS Xu,HuAng ET guO,gEn.nOV.ET SP.nOV.与软水母亚纲多管水母科平枝多管水母zygOCAnnA PlAnATuS Xu,HuAng ET WAng,SP.nOV.。此外,还报道了1种黑圆口水母STOMOTOCA ATrA l.AgASSIz,1862在我国海域首次记录。模式标本保存在国家海洋局第三海洋研究所。The Hydroidomedusae were collected from Taiwan Strait (20°51′-27°03′N, 114°55′-121°14′E) during July 2006 and Jan.2007.Two new taxa Paraeuphysilla taiwanensis gen.nov.et sp.nov.and Zygocanna planatus sp.nov.are described.Stomotoca atra L.Agassiz, 1862 is a new record for Chinese waters.supportedbytheChineseOffshoreInvestigationandAssessmentundercontract(908-ZC-Ⅱ-02);theNationalBasicResearchProgramofScienceandTechnologyofChinaundercontract(2006FY220700);PublicScienceandTechnologyResearchFundsProjectsofOceanundercontract(201005015

肉桂酸对人骨肉瘤MG-63细胞增殖和分化的影响

目的研究中药有效成分肉桂酸对人骨肉瘤MG-63细胞增殖和分化的影响。方法肉桂酸处理MG-63细胞前后,台盼蓝拒染法进行活细胞计数;流式细胞仪检测细胞周期时相变化;光镜和电镜观察细胞形态和超微结构;VanGieson染色检测细胞Ⅰ型胶原表达;茜素红染色观察钙化骨结节形成;免疫细胞化学检测骨粘素和骨钙蛋白表达。结果肉桂酸明显抑制MG-63细胞增殖,D7抑制率达54.94%±4.69%。周期分析结果显示,肉桂酸处理后G0/G1期细胞比例较对照组明显升高(P<0.01)。光镜和电镜结果显示,肉桂酸处理组MG-63细胞形态和超微结构发生明显改变,呈现相应正常细胞的形态和超微结构特征。肉桂酸促进成骨细胞分化标志物Ⅰ型胶原、骨粘素和骨钙蛋白的表达与钙沉积以及典型骨结节的形成。结论肉桂酸可抑制人骨肉瘤MG-63细胞增殖并诱导其向成骨细胞分化

生物胺对雌性锯缘青蟹生殖神经内分泌的调控作用

采用活体注射和体外培养方法 ,进行生物胺调控雌性锯缘青蟹生殖神经内分泌的研究。结果表明 ,锯缘青蟹于实验的第 1天 ,第 5天 ,第 1 0天 ,分别注射 1 0 0 μl剂量为 1 μg/g体重的 5羟色胺 (5 HT)、章鱼胺 (OA)和多巴胺 (DA) ,第 1 5天解剖 ;其中 5 HT对卵巢发育具有显著的促进作用 ,OA和DA的作用不明显。离体条件下 ,将脑、胸神经团、视神经节分别与卵巢小块进行联合培养 ,每个实验组分别加入PBS与 1 μg/g体重的5 HT、OA、DA、HA(组胺 )和NE(去甲肾上腺素 ) ,发现卵巢发育的促进作用与 5 HT刺激了脑和胸神经团的分泌活动有关 【英文摘要】 The possibility that biogenic amines affect ovarian development in the female mud crab, Scylla serrata, was investigated. Females were administered 1μg/g body weight of 5-hydroxytryptamine (5-HT)?octopamine (OA) or dopamine (DA) on days 1, 5 and 10 and were sacrificed on day 15. Crabs given 5-HT showed significant increases in ovarian index and oocyte size over the concurrent controls, while OA and DA did not significantly affect either the ovarian index or oocyte size. In vitro study indicates that none ...福建省重中之重“福建省海洋生物优良种质和生物活性物质的应用基础研究”项目资助 1 998— 2 0 0 2年;; 高等学校博士学科点专项科研基金项目资助 ,2 0 0 1 0 3 84 0 1 0

In vitro study of neuroendocrine regulation over the testicular development in mud crabs Scylla serrata

The reproductive endocrine activities of neuroendocrine organs and androgenic glands (AG) in male Scylla serrata were investigated with co-incubation technology. In vitro studies show that: (1) the AG in Stage Ⅲ can significantly accelerate the development of seminiferous tubules and spermic matu- ration; (2) the brain, thoracic ganglia and optic ganglia have no direct influence over the testicular devel- opment; (3) the brain and thoracic ganglia can significantly promote the growth of the AG cells and hig...This work was supported by the National Natural Science Foundation of China (No.30300269) and the Fujian Province Key Foundation Re-search Program (1998-2002)

锯缘青蟹视神经节存在雄激素受体

国家自然科学基金资助项目 ( 3 0 3 0 0 2 69