Research and Application of Intelligent Shift Technology for Engineering Vehicle Based on Working Spectrum Analysis and Pattern Recognition

随着我国经济的发展，工程车辆在国民经济、社会发展和国防安全等方面发挥着举足轻重的作用。工程车辆的智能换挡技术是工程车辆亟待研究和解决的核心技术，对于保障车辆的动力性的前提下提高经济性、提高作业效率和减轻驾驶员作业强度等方面都有重要的意义。本文以实现工程车辆的智能化为目的，在优先考虑工程车辆动力性基础上兼顾燃油经济性，开展基于作业谱特征提取与模式识别的工程车辆智能化换挡关键技术及应用研究，并通过调控柴油发动机功率模式实现节能降耗。 本文针对工程车辆自动换挡技术存在换挡参数多样、换挡控制目标不合理，提出多参数最优换挡控制目标理论，统一自动换挡控制规律的研究方向，将研究重点调整到换挡控制策略的研究...With the development of China's economy, engineering vehicles play an important role in the national economy, social development and national defense security. Intelligent shift technology is the key technology to study and solve of the engineering vehicle, it is of great significance to improve the economy, improve the efficiency of the operation and reduce the intensity of the driver's work. In ...学位：工学博士院系专业：航空航天学院_精密仪器及机械学号：1992011015403

关于疏肝-健脾-疏肝健脾方与肝郁脾虚证相关的研究之思考

1课题的学术背景及研究思路“方证相关“中医辨证论治的核心内容之一,反映方剂与病证之间的适配性或方与证之间的关联性,其所蕴涵的重要科学问题是方与证间的关联性大小。进行方证关系的研究必须考虑到方药组成和所主病证两个方面。因此复制适宜的可供研究用的方证模型和选择在功效内涵和药物组成上具有一定关联的方药是开展方证关系研究的基础。本研究则是以肝郁脾虚证及其相关治方为切入,从异方同证的角度,观察中医疏肝方柴胡疏肝散、健脾方四君子汤、疏肝健脾方柴疏四君汤对同一肝郁脾虚证模型的作用。国家自然科学基金项目(No.10002902-233-12

浅谈介壳类药对在妇科中的应用

介类动物在《本草纲目》中分为龟鳖类和蚌蛤类,共15种。现临床上多以介类动物之外壳入药,如牡蛎、海螵蛸、龟板、鳖甲、石决明、珍珠母、瓦楞子、海蛤壳等等。中药主要来源为植物,介壳类中药为数不多,介壳类药对则更少,但在临床中应用广泛,疗效独到,历代文献皆有记载。本篇就妇科常用的介壳类药对作归纳探讨

DSP-based Control System for Oil Field Water Injection

介绍一种以dSP(数字信号处理器)为核心的油田注水智能实时控制系统。该系统通过对油井压力的监测,将采集到的压力值通过CAn总线传送给dSP,dSP根据压力值的变化按照预先设置的参数控制阀门开关,并利用流量计监测流量,再根据流量值的大小变化,进一步的调节水流量,实现油田的智能注水。与此同时,利用SC I总线将压力值和流量值送到PC机上,再运用gPrS模块将实时监控数据传送到远程的主机上,实现远距离的自动控制。In this paper,an intelligent real-time control system based on DSP(digital signal processor) for oil field water injection is introduced.The pressures of wells are monitored and collected,then through the CAN bus pressures are transmitted to the DSP.Based on the pressure changes,the DSP controls the valve in accordance with the pre-set parameters.The flow is monitored by flowmeters.Then,the water flow is further regulated depending on the monitored data.Thus,the intelligent oil field injection is realized.At the same time,the pressure and the flow value are sent to the PC machine through the SCI bus,and then sent to a remote host by using GPRS modules to achieve longdistance automatic control

Proliferative and Chondrogenic Potential of Peripheral Blood Mesenchymal Stem Cells in Demineralized Cancellous Bone Scaffolds

目的：研究外周血来源的间充质干细胞（PBMSCs）在3D多孔支架中的生物学行为。方法：将兔PBMSCs接种到猪脱矿松质骨（DCB）支架中,并以骨髓间充质干细胞（BMMSCs）以及关节软骨细胞（ACCs）进行对照,通过扫描电镜观察3D环境中的细胞形态和分布;通过Live/Dead染色检测细胞活性,Hoechst33258法检测DNA含量,二甲基亚甲基蓝（DMMB）法检测糖胺聚糖（GAG）含量,酶联免疫吸附试验（ELISA）和免疫荧光法检测二型胶原（COL 2）表达、RT-PCR法定量分析软骨分化相关基因的表达。结果：扫描电镜（SEM）观察显示3种细胞在支架中均匀贴附,Live/Dead染色显示接种3天后3种细胞存活率相似（P〉0.05）,7天后3种细胞增殖能力以及DNA含量无明显差异（P〉0.05）。PBMSCs和BMMSCs均较ACCs分泌更多GAG,但COL 2分泌量相似。此外,PBMSCs和BMMSCs的聚集蛋白聚糖（AGC）,COL 2和碱性磷酸酶（ALP）等基因表达均明显上调（P〈0.05）;而ACCs组这些基因的表达显著下调（P〈0.05）。MSCs组的COL 1表达趋于增加,而ACCs组中的表达趋于减少（P〉0.05）。在成软骨诱导21天时,PBMSCs和BMMSCs的COL 2和ALP表达较ACCs升高（P〈0.05）,AGC和COL 1表达与ACCs相比无显著差异（P〉0.05）。结论：外周血来源的间充质干细胞在异种脱矿松质骨支架中具有与骨髓间充质干细胞相似的良好的增殖和成软骨能力,但体外培养条件下依然存在肥大软骨细胞基因表达的现象,需要对培养条件进一步优化。Objective To study the biological behavior of peripheral blood mesenchymal stem cells（PBMSCs） in 3 D composite scaffolds. Methods The proliferation and chondrogenesis of rabbit PBMSCsseeded on porcine cancellous bone（DCB） scaffolds were evaluated,and bone marrow mesenchymal stem cells（BMMSCs） and articular chondrocytes（ACCs） were used as controls. Cell morphology anddistribution in scaffolds were observed using scanning electron microscopy（SEM）. Live/Dead stainingwas employed to detect cell viability,Hoechst 33258 method to measure DNA content,dimethylmeth-ylene blue（DMMB） assay to detect glycosaminoglycan（GAG）,enzyme-linked immunosorbent assay（ELISA） and immunofluorescence to detect the content of type 2 collagen（COL 2）,and RT-PCR toanalyze chondrogenesis-related gene expression. Results SEM showed that three kinds of cells uniformlyadhered and evenly distributed in DCB scaffolds. Live/Dead staining observed the similar viability ofthe three kinds of cells three days after seeding（P〈0.05）. There was no significant difference in theproliferation ability and DNA content among three kinds of cells after seven days of in vitro culture.After 21 days of chondrogenic culture,both PBMSCs and BMMSCs secreted more GAGs than ACCs,while the secretion of COL 2 was similar to that of ACCs. Moreover,the gene expression of AGC,COL2 and alkaline phosphatase（ALP） were significantly up-regulated（P〈0.05） in PBMSCs and BMMSCsbut significantly down-regulated in ACCs（P〈0.05）. The expression of COL 1 in MSCs groups dis-played an increasing trend but a decreasing trend in ACCs group（P〈0.05）. The gene expression ofCOL 2 and ALP,but not of AGC and COL 1,in PBMSCs and BMMSCs was higher than those inACCs（P〈0.05）. Conclusions PBMSCs and BMMSCs have similarly excellent proliferation and chondro-genesis potential in 3 D porous DCB scaffolds. However,hypertrophic gene expression is still observedunder in vitro culturing condition,suggesting the need to further optimize the cu国家自然科学基金面上项目（51273004

Load Spectrum Compiling Based on Operation Section Recognition and Fatigue Life Prediction of Wheel Loader Axle Shaft

为改进轮式装载机可靠性及耐久性设计中数据清洗和分析的方法,以装载机半轴为对象,优化载荷信号去噪和作业段识别的方法。利用小波自适应阈值法去除实测载荷的噪声尖峰和突变信号,并通过迭代平滑滤波法搜寻单个作业周期内同步载荷信号的极值点,识别、划分作业段为＂行走段-铲掘段＂。结合雨流计数和频次外推法编制载荷谱,有限元静力分析,以及Miner准则对装载机半轴进行疲劳寿命分析。结果表明：雨流计数的均幅值结果符合正态分布和三参数威布尔分布的假设性检验,疲劳分析损伤最大节点出现在万向节叉连接处,其寿命为7.22×10^8次循环。结果证实了作业段智能识别法的便利性和准确性,为传动系统疲劳分析的数据处理提供了借鉴。In order to improve the method of data cleaning and data analysis in fatigue durability and modify reliability design of wheel loader,research on load signal denoising and operation section recognition of wheel loader axle shaft was conducted. In view of the shortcoming of residual noise and waveform distortion caused by traditional wavelet threshold analysis,an adaptive threshold denoising method was used. Operation sections were divided as＂driving section-shoveling section＂through searching the extreme points of synchronous load signal in one single work cycle after iterative smoothing filter. Based on one-dimensional program loading spectrum and the finite element analysis,the fatigue life of axle shaft was predicted using the Miner criterion. The result shows that rain flow counting results were consistent with Normal distribution and Weibull distribution hypothesis testing. And the maximum damage node appeared in the universal joint folk which fatigue life is 7. 22 × 10^8 times cycle. The result confirmed that the intelligent recognition method is convenient and accurate,which provides a reference method for data processing of fatigue analysis of wheel loader transmission system.福建省财政厅企业技术创新项目资

李学麟教授善用桂枝汤的临床举隅

李学麟,福建中医药大学教授,主任医师,被国家中医药管理局授予第一批“全国优秀中医临床人才“的称号。李师幼承庭训,熟读经文,博采众方,首推内经,亦重伤寒,善用桂枝,验之临床,其效桴鼓,今愚揣鄙陋,愿笔下点滴,供同道指正。桂枝汤源自汉代医圣张仲景的《伤寒杂病论》,具辛温解表之功,适用于头痛发热,汗出恶风,鼻鸣干呕,苔白不渴,脉浮缓或浮弱者的外感风寒表虚证。李师在临床实践中,体会桂枝汤,具滋阴和阳,解肌发汗,

Mechanism of geniposide in improving free fatty acid metabolism in rats with non-alcoholic fatty liver disease

观察栀子苷改善非酒精性脂肪性肝病的效果,从游离脂肪酸探讨栀子苷调节非酒精性脂肪性肝病的作用机制。健康雄性Wistar大鼠40只,随机分为4组:正常组、模型组、栀子苷组和血脂康组,每组大鼠10只,正常组大鼠给予正常大鼠饲料喂养,其余3组大鼠均采用高脂饲料喂养以诱导非酒精性脂肪性肝病,造模时间为8周,从第5周起至第8周末,栀子苷组和血脂康组分别灌服相应的药物。记录大鼠体重、肝湿重、脂肪质量;用相应方法检测肝组织TG,FFA,FAS,AMPK,ACCase及Malonyl-Co A含量,血清CHO,LDL-C的含量,血清AST,ALT的活性;观察肝脏组织肉眼及病理变化(HE染色法)。结果显示,与正常组相比,模型组大鼠的体重;肝湿重;脂肪重;血清CHO,LDL-C,ALT,AST;肝组织TG,FFA,FAS,ACCase及Malonyl-Co A含量皆显著升高(P<0.01);肝组织AMPK活性显著降低(P<0.01),肝组织肉眼外观及病理切片脂肪变性明显,并出现炎症损伤;与模型组相比,栀子苷组大鼠体重、脂肪质量、肝组织FFA含量、血清ALT,AST活性均显著降低(P<0.01),肝湿重,肝组织TG,FAS,ACCase及Malonyl-Co A含量明显降低(P<0.05),肝组织AMPK活性明显增多(P<0.05),肝脏肉眼外观及病理学表现均有所改善;与模型组相比,血脂康组大鼠的肝湿重、脂肪质量、肝组织TG,FFA和血清LDL-C水平明显降低(P<0.05);与血脂康组相比,栀子苷组大鼠的体重、脂肪质量、肝组织FFA含量均显著降低(P<0.01),其他方面无明显差异。结果表明,栀子苷具有显著的改善高脂饮食诱导的大鼠非酒精性脂肪性肝病的药理效应;其改善大鼠非酒精性脂肪性肝病的游离脂肪酸代谢是通过调节"AMPK-ACCase-Malonyl-Co A-FFA"轴来实现的。To observe the effect of geniposide on non-alcoholic fatty liver disease( NAFLD),and discuss the mechanism of geniposide for NAFLD from the aspect of free fatty acid,forty healthy Wistar male rats were randomly divided into normal group,model group,geniposide and Xuezhikang group. The rats in normal group were fed with normal diets,and the rats in other 3 groups were given with high-fat diet for 8 weeks to induce the NAFLD models. From the week 5 to end of week 8,the rats in geniposide and Xuezhikang group were intervened with corresponding medicines. The body weight,liver wet weight,and fat weight of the rats were recorded.Visual and pathological changes in hepatic tissues were observed with HE staining. The contents of TG,FFA,FAS,AMPK,ACCase and Malonyl-Co A in hepatic tissue,contents of CHO and LDL-C in serum and activities of AST and ALT in serum were detected by using corresponding methods. The results showed that the body weight,liver wet weight,and fat weight of the rats,CHO,LDL-C,ALT and AST levels in serum,TG,FFA,FAS,ACCase and Malonyl-Co A levels in hepatic tissues of the rats in model group were significantly higher than those in normal group( P < 0. 01),while AMPK activity was significantly lower than that of the normal group( P < 0. 01),with obvious visual and pathological steatosis in hepatic tissues,and inflammatory injury occurred in model group. Compared with the model group,body weight of the rat,fat weight,levels of FFA in hepatic tissues,ALT and AST activities in serum,liver wet weight,TG,FAS,ACCase and Malonyl-Co A levels were significantly decreased in geniposide group( P < 0. 01),while the AMPK activity in hepatic tissues was significantly increased( P < 0. 05),with improvement in visual and pathological performance.Compared with the model group,liver wet weight,fat weight,TG and FFA levels in hepatic tissues,and LDL-C level in serum were significantly decreased in Xuezhikang group( P < 0. 05). Compared with Xuezhikang group,the body weight of rat,fat weight and FFA level in hepatic tissues were significantly lower in geniposide group( P < 0. 01),but with no significant difference in other aspects. These findings indicated that geniposide was highly effective in improving the pharmacological effect of NAFLD induced by highfat diet,and the mechanism was achieved through AMPK-ACCase-Malonyl-Co A-FFA axis.国家自然科学基金项目(81274155;81503529);; 福建省自然科学基金项目(2014J01374);; 福建省高等学校新世纪人才计划项目;; 厦门市科技计划项目(3502Z20134020);; 福建省中医药科研项目(WZPW201308

人骨髓间充质干细胞分离培养方法的改进

背景:当前分离培养骨髓间充质干细胞方法的有多种,但在外科手术中进行获取的方法仍然较少,而外科分离所得细胞对于相应疾病的具有揭示原因和提供研究线索的价值。目的:在骨科手术中以直接贴壁差速贴壁分离培养纯化与鉴定骨髓间充质干细胞。方法:在骨科关节置换等大手术中吸取少量人骨髓血,采用直接贴壁法分离骨髓间充质干细胞,在贴壁后36～48h行洗盘处理,并通过长期体外培养,特定培养基自身的筛选作用对细胞进行筛选,当细胞生长达到足量时进行鉴定。结果与结论:采用直接贴壁法能够在长骨髓腔内混合血中分离培养骨髓间充质干细胞并形成集落,其形态学表现和表面分子经鉴定符合骨髓间充质干细胞特点,能够获得符合要求的人骨髓间充质干细胞,从而进行后续实验

Pressure Measurement of Vacuum Insulation Panel with Infrared Spectroscopy

真空绝热板(VIP)主要是依靠其内部真空度来提高其绝热性能的,由于导热系数是表征真空绝热板绝热性能最重要的物性参数,准确测量出真空绝热板的导热系数,有助于将真空绝热板应用于需要绝热保温的场合并估算出其热负荷情况,为系统选择制冷设备或加热设备提供依据。在对真空绝热板真空度检测技术的研究中,本文致力于研究一种基于热红外技术的真空绝热板真空度检测方法,主要采用埋入热辐射器件、能量由外部无损耦合提供、使用红外热图像处理技术并结合电路控制系统等实现对真空绝热板的真空度检测。实验表明,红外检测技术用于测量真空度是可行的、检测速度快、检测结果可靠;对热红外检测技术的研究有利于推动国内真空度快速检测技术的发展。A novel technique has been successfully developed to non-destructively evaluate the pressure in the vacuum insulation panel( VIP),fabricated on industrial scale.In the newly-developed technique,the heat radiation device,embedded in the VIP and energized in non-contact mode by the lab-built,external power-supply,is capable of emitting infrared( IR) light.The internal pressure can be indirectly estimated via data analysis of the spectrum and image acquired with a digital IR camera.The impact of the time evolution of the pressure on the IR image was experimentally and analytically simulated.The preliminary results show that the newly-developed,IR pressure-probe is feasible to measure rapidly the VIP pressure with high reliability.Further improvement of its measurement accuracy was under way