Comparative proteome of different ecotypes of reed (Phragmites communis Trin.) adapted to natural droght and saline habitats

芦苇是禾本科多年生水生草本植物，其典型生境为淡水、含盐的沼泽或湖边，然而，芦苇可适应多种生境条件，并演化为对干旱、盐渍或低温等陆生胁迫环境有较强抗旱性的、具有基因型差异的不同生态型。我国河西走廊荒漠地区广泛分布有水生芦苇、盐生芦苇、盐渍-沙丘过渡带芦苇和沙丘芦苇4个不同生态型，这一长期稳定的自然变异生态型式研究植物适应逆境机制的理想材料体系。为了从分子水平上确证由生理生化及生态学方面综合研究结果推定的4个生态型的演化趋势，本研究首先采用ISSR及RAPD分子标记方法对四种不同生态型芦苇进行了遗传多样性分析，在此基础上，运用蛋白质组学技术并结合生物信息学方法，对不同生态型芦苇叶片全蛋白及叶绿体亚...In the deaert regions of northwest China, Hexi Corridor of Gansu provice , Phragmites communis, a hydrophytic species whose typical habitats are the fresh and brackish water area of swamp and riversides, adapted itself to adverse terrestrial habitats and evolved into other three terrestrial reed ecotypes under natural selection pressure, and thus developed a unique habitat distributing four reed e...学位：理学硕士院系专业：生命科学学院生物学系_发育生物学学号：20042604

Study on the Extended Gini Coefficient and Its Application

本文给出了拓展基尼系数的一个等价定义,给出拓展基尼系数组群、要素分解的统一形式。对2009年我国城乡居民食品消费数据进行结构性分析,结果表明:1拓展基尼系数是不平等厌恶参数的增函数,最小值为0、最大值为1-S1/P1;2随着不平等厌恶参数的增大,各个组群或要素对不平等边际效应的方向保持相对稳定;3城镇居民在外餐饮、肉禽及制品、水产品类支出份额的增加,对食品消费不平等产生消极的边际影响。This paper promotes an equivalent definition of the extended Gini coefficient,and proposes an unite decomposition formula of the extended Gini coefficient by subgroups and factors.Applying the formula to 2009 China's food consumption data yields several interesting results as follows: ①the extended Gini coefficient is an increasing function of aversion inequality parameter,with the minimum 0 and the maximum 1-S1/p1;②with the parameter increasing,the relative marginal effects of subgroups or factors on the extended Gini coefficient have rather stable action direction;③it will has a negative effect on food expenditure inequality to increase dining out,meat,poultry and processed products,aquatic products expenditure ratio.国家社会科学基金“教育公平与效率统计测度及其关系的实证研究”(BFA090018)资助;福建省统计学重点实验室(厦门大学)的资

The study of an effect on the motor learning of equilibrium functions in pre-school children

We made a new apparatus, named the Balance meter, to make it clear that pre-school children show the states of equilibrium functions, which are remarkable for development of a motor nerve, the properties of this apparatus possess both the role of a measurement system and of a playmaterials. By using this, therefore, it is interested in that how balance will be performed. To investigate the problems, two similar groups of 5 subjects at four and five years of age were formed, (especialy within ± 1/2 σ in body height, weight and behaviaral attitudes, etc.) Moreover the some acquired states of equilibrium functions were measured after a week the motor learning task was over. The results obtained in this study are as follows; 1) The reliability of a new upparatus is recognized from the results which showed asignificant correlation in the case of the first time as well as the second measuremant. (r=.802) 2) The experimental groups showed rapidly improvement at the records of the Balance meter, while, the control groups were not found out some changes at the behaviaral attitudes ; we say that equilibrium functons in pre-school children is educability, trainability, and it isn\u27t unchangeability. 3) The performance process of the motor learning at five years of age is greater the effect than that at four, and females than males. From those results, we.say that in this time children have to be dealed with the methods of the level of their development

不同生态型芦苇叶片蛋白质双向电泳系统的筛选和优化

通过优化组合植物蛋白质提取方法及与之匹配的蛋白质裂解液，采用改进的O′Farrel 双向电泳系统，以自然生境野 生芦苇叶片为材料，筛选出一种适合纤维含量高、革质化明显的4 种不同生态型芦苇(水生芦苇、轻度盐化草甸芦苇、重度 盐化草甸芦苇、沙丘芦苇)叶片蛋白质分析的双向电泳系统，即以饱和酚- 醋酸铵/ 甲醇沉淀法提取叶片蛋白质样品，经裂解 液[8 mol/L 尿素，2 mol/L 硫脲，4% CHAPS，65 mmol/L DTT，2% Ampholine(pH3.5~10∶pH5~8 = 1∶4)] 裂解后按80 !g 上样，银染后获得背景清晰、蛋白质分辨率较高的双向电泳图谱. 该系统用于水稻等植物叶片蛋白质双向电泳分析，同样获 得较好的电泳图谱和分辨率.国家自然科学基金(30470164), 厦门大学新世纪优秀人才支持计划资助项目(NCETXMU)和福建省自然科学基金(C0410001)资助项

海洋酸化对优势固氮蓝藻束毛藻的影响及其机理

由人为活动排放的二氧化碳（CO2）而引起的海洋酸化,预计将影响对热带、亚热带海域的初级生产力有显著贡献的固氮浮游植物。本研究揭示了海洋酸化对优势固氮蓝藻束毛藻（Trichodesmium spp.）的生长和固氮的影响及其机理。研究表明,虽然海洋酸化中的CO2升高对束毛藻有促进效应,但酸化的整体效应为抑制其固氮和生长。究其原因,海洋酸化引起束毛藻胞质pH下降,从而降低固氮酶效率、干扰胞内pH稳态、影响细胞产能。在铁限制条件下,由于有限的铁难以满足束毛藻响应海洋酸化胁迫所做出的生理响应,因而铁限制加剧了海洋酸化对束毛藻的负面影响

Research Progress in Chloroplast Proteome

作者简介: 彭 浩(1982-),女(汉族),硕士,主要从事植物蛋白质组学方面的研究。 通讯作者: 朱学艺, 博士, 副教授, 硕士生导师, 主要从事植物响应环境因子的功能蛋白质组学研究。E-mail: [email protected].[中文文摘]亚细胞蛋白质组学是近年来蛋白组学研究中的一个热点。通过细胞器的纯化和亚细胞组分的分离,降低了样品的复杂性,增大了相应蛋白质组分的富集,有利于由此分离获得的蛋白质的序列分析及功能鉴定。叶绿体蛋白质组为植物亚细胞蛋白质组学研究中相对全面的一部分,利用亚细胞分离结合双向电泳技术系统地鉴定叶绿体中蛋白质组分是获取叶绿体蛋白质信息、确定其功能的重要技术手段。本文就近年来植物叶绿体蛋白质组涵盖的叶绿体内、外被膜、叶绿体基质、类囊体膜和类囊体腔蛋白的研究进行综述,以全面认识叶绿体蛋白的组成、特点及其在叶绿体生理生化代谢网络中的作用。[英文文摘]Subcellular proteomics is currently the most effective approach to characterize subcellular compartments.Based on the powerful combination of subcellular fractionation with proteomic analysis,it makes possible to characterize all chloroplast proteins completely.Chloroplast proteome is one of the most intensive aspects of the plant subcellular proteomics research.By using subcellular proteome technique,more and more choloplastic proteins have been identified.The present review summarizes the recent progress in proteins located in the different chloroplastic compartments,including the inner and outer envelop membranes,chloroplast stroma,thylakoid membranes and thylakoid lumen.We hope it contributes to understand the characters of chloroplastic proteins and their functions in the chloroplastic metabolism networks.国家自然科学基金(30470164);厦门大学新世纪优秀人才支持计划项目(NCETXMU