180 research outputs found

    The strategic ideas for sustainable development and industrialization of agriculture in the Northeast of China

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    阐述了区域农业可持续发展的涵义,并在东北农业产业化现状及存在问题分析的基础上,探讨实现东北农业可持续发展的有效途径,以及可持续农业产业化建设应遵循的原则和相应对策。参7。This paper expatiates on the meaning and contents of sustainable agriculture development in region.Based on the analysis of present situation and problems existed in industrialization of agriculture in the Northeast of China,the effective approaches for sustainable agriculture development and the rules as well as strategies for sustainable agriculture industrialization were discussed.国家“十五”科技攻关重点项目(2001BA508B05

    Experimental Study of the Abalone Shell on Cataract induced by Oxidative Stress

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    目的研究石决明提取物对体外培养的晶状体氧化应激性白内障形成的作用及机制。方法离体培养小鼠晶状体,应用不同浓度的石决明提取物预孵育晶状体24h后,加入1mm过氧化氢,继续培养3小时后恢复正常培养,72小时后观察小鼠晶状体混浊程度,测定晶状体组织培养液中的乳酸脱氢酶(LDH)含量,晶状体组织中还原型谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力。结果石决明提取物在1~2 mg/ml浓度范围内减轻氧化应激造成的晶状体混浊,减少晶状体LDH的释放,提高组织内GSH含量和SOD活力。结论石决明提取物可减轻氧化应激白内障的形成,其作用主要与石决明提取物提高内源性抗氧化系统有关。Objective To study the effect of the abalone shell extract on oxidative stress induced cataract formation and its mechanism in cultured mouse lens in vitro. Methods The cultured mouse lens were pretreated with the abalone shell extract in different concentrations for24 hours,and then 1mm hydrogen peroxide was added and continued incubating for 3 hours,and they were changed to normal culture media.After 72 hours,the opacity of each lens was observed under an anatomical microscope,the content of lactate dehydrogenase( LDH)leakages,the content of the reduced glutathione( GSH) and activity of superoxide dismutase( SOD) in lens tissue were assayed. Results Abalone shell extract in the concentration range of 1 ~ 2 mg / ml reduced the lens opacity caused by oxidative stress,alleviated the release of LDH,and increased GSH content and SOD activity in cultured lens. Conclusion Abalone shell extract can alleviate the oxidative stress induced cataract formation,and this effect is mainly related to its improvement of the endogenous antioxidant system in lens.2012年福建省卫生厅中医药科研专项课题(No.WST201210);; 2013年福建省卫生厅中医药科研专项课题(No.wzhw201302);; 2014年厦门市科技局科技惠民项目课题(No.3502Z20144030

    九龙江口营养盐的分布、通量及其年代际变化

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    于2008年4月至2011年4月对九龙江河口的营养盐进行了8个航次的调查,包含丰水期(5~9月)和枯水期(10月~次年4月).结果表明,九龙江口上游营养盐浓度很高(硝酸盐nO3-n120~230μMOl/l,亚硝酸盐nO2-n5~15μMOl/l,氨氮nH4-n15~170μMOl/l,磷酸盐SrP1.2~3.5μMOl/l,硅酸盐dSI200~340μMOl/l),且枯水期高于丰水期.nO3-n是溶解态无机氮(dIn)的主要组分,枯水期nO3-n可占dIn的55%~72%,而丰水期该比例则高达67%~96%.nO3-n和dSI在盐度1~32的河口混合区基本呈现保守混合行为.SrP在盐度1~25的区域浓度变化很小(1.0~2.0μMOl/l),在盐度>25的区域则被相对低营养盐的近海海水所稀释.利用高分辨率的径流量资料和淡水端实测营养盐浓度,计算出九龙江dIn,SrP和dSI的入河口通量分别为34.3x103Tn/A,0.63x103TP/A和72.7x103TSI/A;而通过将营养盐在高盐段的保守混合规律外推至零盐度估算河口输出有效浓度的方法,估算出九龙江口营养盐的入海通量分别为34.8x103Tn/A,0.82x103TP/A,71.6x103TSI/A.利用lOICz推荐的营养盐收支模式方法,估算调查期间九龙江口对SrP的添加量为0.16x103TP/A.与世界其他河流对比,九龙江流域单位面积上的nO3-n产率处于较高水平.与历史数据相比,近10余年来九龙江口上、中游nO3-n和SrP的浓度增加2~3倍,而dSI浓度在九龙江口盐度梯度上的分布则改变不大.这种营养盐年代际变化与密西西比河dIn和SrP升高而dSI浓度大幅度下降的变化特征很不相同.国家自然科学基金(40810069004);国家重点基础研究发展计划(2009CB421204)资助;国家海洋局海洋环境保护司资助项目(DOMEP-MEA-01-10);国家自然科学基金委2010年台湾海峡科学考察实验研究(40949901)的支

    全体討論[11月22日]

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    文部科学省私立大学学術研究高度化推進事業オープン・リサーチ・センター整備事

    Experimental study on reperfusion of intraocular lens

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    作者简介: 祁明信, 男, 1945 年7 月 出生, 教授、主任医师、博士研究生 导师, 主要从事白内障的基础与临 床研究。联系电话: 0591-83570887; E-mail:qihuang@netease. com 通讯作者: 黄秀榕,E-mail:[email protected][中文文摘]目的开展晶状体再灌注的离体和动物实验研究,并对再灌注人工晶状体技术进行评价。方法采用新鲜离体幼兔眼、离体猪眼、新西兰白兔眼,应用自行研制的人工晶状体材料,进行以下实验:(1)体外固化实验;(2)晶状体前囊膜微型撕囊及其稳固性实验;(3)经微型前囊膜开口超声乳化吸出晶状体内容物实验;(4)活的新西兰白兔眼内人工晶状体再灌注实验。结果(1)按硅酮聚合物与固化剂50:1的比例可获得柔软、弹性好、固化时间短(完全固化时间为60min)的注入材料;(2)晶状体前囊膜1.8~2.0mm的连续环形撕囊口具有较好的稳定性,可经该微型开口吸出晶状体内容物并灌注材料;(3)超声能量18%、流量25mL·min-1、负压120mmHg(1kPa=7.5mmHg)为晶状体内容物经微型前囊膜开口吸出的最佳条件;(4)注入灌注材料后可形成由晶状体囊膜包裹的、置换原晶状体皮质和核的、新的再灌注人工晶状体。结论采用再灌注人工晶状体的方法可进行新型人工晶状体再灌注,可为治疗白内障和老视提供参考。[英文文摘]Objective To carry out the experimental study on reperfusion of intraocular lens(IOLs) in vitro or in animal,and to assess the technique of IOLs reperfusion.Methods The following experiments were performed by using self-developed materials in fresh rabbit eyes and pig eyes in vitro,as well as in eyes of alive New-Zea-land rabbits:(1)Solidification study of self-developed material in vitro;(2)Continuous circular capsulorhexis(CCC) in anterior capsule of lens and its stability;(3)Draw of lens contents via phaco through mini-CCC;(4)IOLs ref illing in the eyes of alive New-Zea land rabbits. Results(1) Thematerialwhich was soft, springy and short-term solidification(full solidification time was 60 minutes) were obtained in certain proportion of geland solidified agent(50:1) in vitro; ( 2)The CCC in anterior capsule of lens with 1.8-2.0 mm diameter had very good stability. The lens contents were drawn and the materialwere refilled through themini-CCC; (3) The best conditions of drawing out lens contents through m ini-CCC were phaco energy 18% , flow 25 mL·min- 1, and negative pressure 120 mmH g (1kPa=7.5 mmHg);(4) The new refilled IOLs, which were wrapped by capsule of lens and were replaced original cortex and nucleus of lens, were obtained after thematerial refillied. Conc lusion. New IOLs are refilled through this method, which can prov ide reference for the treatment of cataract and presbyopia.福建省科技三项费用;教育厅重点资助项目基金资助(编号:K98041
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