Callus Induction and Plant Regeneration in Callistemon rigidus

以茎段、芽和叶片为材料,研究了红千层愈伤组织诱导及植株再生的方法。结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养愈伤组织可发育成绿色和粉红色2种类型,其中绿色、致密的愈伤组织可以分化出不定芽。培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%。Taken stem segment,bud and leaf as materials,the means of callus induction and plant regeneration in Callistemon rigidus R.Br.were studied.The results showed that callus could be induced from all types of the materials,pink and green callus could be gain through subculture,and adventitious buds could be induced from the compacted greens.The optimal medium for inducing adventitious bud was 1/2MS supplemented with 6-BA1.0 mg/L and NAA0.1 mg/L,and the optimal one for test-tube plantlets rooting was 1/2MS supplemented with IBA0.25 mg/L,in which the rooting rate could be up to 95%.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361

Callus Induction and Plant Regeneration in Callistemon rigidus

以茎段、芽和叶片为材料,探讨了红千层愈伤组织诱导及植株再生的方法.结果表明:红千层的茎段、芽和叶片均可诱导出愈伤组织,通过继代培养可发育成绿色和粉红色2种类型的愈伤组织,其中绿色、致密的愈伤组织可以分化出不定芽;培养基1/2M S+6-BA 1.0 m g/L+NAA 0.1 m g/L适宜愈伤组织不定芽的诱导,在培养基1/2M S+IBA 0.25 m g/L上试管苗的生根率可达95%.Taking stem segment,bud and leaf as materials,this paper made a study of callus induction and plant regeneration in Callistemon rigidus R.Br.The results show that callus can be induced from all types of materials and after being sub-cultured,the callus will develop to two kinds of colors that are pink and green;that the pink callus will be degenerated while the compact green one will induce adventitious buds.The optimal medium for adventitious bud inducing is 1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L and the optimal for the rooting of test-tube plantlets is 1/2MS+IBA0.25 mg/L on which its rate can reach 95 % after being cultured for 40 days.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(004Z3-E0361)的部分研究内

Stem Culture and Rapid Propagation of Melastoma malabathricum Linn.

广州市科技计划项目(2002Z3-E0291

Seeds Culture of Anoectochilus roxburghii

金线莲种子在培养基1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L上萌发后形成原球茎,原球茎可以直接发育成幼苗,也可以由原球茎产生愈伤组织,再由愈伤组织发育成类原球茎而分化成幼苗。通过类原球茎可以实现大量增殖,在MS+6-BA1.0 mg/L+NAA0.1 mg/L上培养60 d的增殖倍数达到6.7倍。在培养基MS+IBA0.3mg/L上,金线莲的生根率可达到96.0%。Seeds of Anoectochilus roxburghii would germinate and develop to protocorms on the medium of 1/2MS+6-BA1.0 mg/L+NAA1.0 mg/L.The protocorms may develop to seedlings immediately or induce callous and then induce protocorm-liked-bodies which would develop to seedlings also.Anoectochilus roxburghii be rapidly propagated by the later method and 6.7 folds of propagation would get on the medium of MS+6-BA1.0 mg/L +NAA0.1 mg/L after subcultured 60 days.Seedling′s rooting rate was 96.0% on the medium of MS+IBA0.3 mg/L.广东省科技计划项目(2004B60302006);; 广州市科技计划项目(2004Z3-E0361);; 广州市建委项目[(2003)06

Identification of stem-like cells in non-small cell lung cancer cells with specific peptides

Recent studies indicate that tumor maintenance, metastasis and drug-resistance are mainly conducted by a small subset of cancer cells which are termed cancer stem cells (CSCs) or cancer stem-like cells (CSLCs). Successful identification of CSCs/CSLCs might lead to discovery of the novel and effective therapeutic targets for cancers. In our study, lung CSCs/CSLCs were enriched by sphere-forming assay. Screening and selection of specific binding peptides for lung CSCs/CSLCs were performed with bacterial surface display method. Selected peptide named HCBP-1 exhibited highest specific binding capability as examined by flow cytometry and fluorescence microscopy. Drug-resistant lung CSCs/CSLCs might be characterized with HCBP-1 peptide and several microRNAs related to the stem-like properties were discriminatively expressed in HCBP-1(+) subpopulation. Moreover, at least two distinct subpopulations in H460 tumor sphere cells could be distinguished by HCBP-1 peptide. Thus, a new method was established to identify lung CSCs/CSLCs, which provided robust approaches for the research of CSCs/CSLCs. (C) 2014 Elsevier Ireland Ltd. All rights reserved

Heterogeneity in cancer stem cells

Accumulating evidence suggests that cancer stem cells (CSCs) are heterogeneous populations and their phenotypes are unstable. A number of intrinsic and extrinsic mechanisms contribute to CSC phenotypic variation. The existence of various CSC subpopulations which would lead to a rapid relapse after primary treatments might pose a problem for CSC targeted therapeutics. In order to develop more effective approaches to cancer therapeutics, more CSC-related surface markers or targeting molecules, as well as some novel targeting strategies should be explored. This review summarized the origin and performance of heterogeneity in CSCs and discussed their therapeutic implications. (c) 2014 Elsevier Ireland Ltd. All rights reserved