氢化物发生技术中化学干扰的研究进展

引言在氢化物发生技术与原子光谱分析方法的结合中,基体元素的干扰效应可分为二类:(1)在还原过程中氢化物生成效率的改变引起的干扰;(2)已被还原为氢化物的元素在原子化过程中形成稳定化合物而引起的干扰。可统称为化学干扰。在氢化物发生原子吸收光谱分析中,这二类干扰都是比较显著的。当使用感耦等离子体作激发光源时,由于等离子体的温度很高,而且送入等离

Synthesis of 4-substituted-7-chloroquinoline derivatives and preliminary evaluation of their antitumor activity

目的:设计合成一系列4-取代-7-氯喹啉衍生物并测定其抗肿瘤活性。方法:4,7-二氯喹啉通过烃化反应及缩合反应得到目标化合物,并采用MTT法,评价了其对4种体外培养肿瘤细胞的生长抑制作用。结果:大多数化合物具有一定的抑制肿瘤细胞增殖的活性,其中化合物3,5和10A抑制人非小细胞肺癌A549,nCI-H446,HElA细胞生长的作用强于阳性对照,化合物5和10A对于人非小细胞肺癌A549的抑制活性是阳性对照药的4~5倍,明显优于阳性对照。结论:本文合成的该系列部分化合物具有较好的抗肿瘤活性,是潜在的抗肿瘤候选药物。Objective: To design and synthesize a series of 4-substituted-7-chloroquinoline derivatives,and to assay their antitumor activity.Methods: The compounds were synthesized through alkylation and condensation reaction using 4,7-dichloro-quinoline,and the antitumor activities were assayed by MTT.Results: Most synthesized compounds had the property of antiproliferation on tumor cell lines in vitro.It was obvious that the compounds of 3,5 and 10a had stronger effect on the growth of A549,NCI-H446 and Hela cancer cells than the positive control group does,and compounds 5 and 10a also showed 4 ~ 5 times more inhibition effect on the A549 cells than the positive control group in terms of antitumor activity.Conclusion: The series of synthesized compounds exhibit excellent antitumor activities and deserve consideration for potential candidate of antitumor drugs泉州市科技计划重点项目(2013Z35

Effect of rutile content on photocatalytic water-splitting for hydrogen production over composite-crystal nano-TiO_2

合成了不同金红石相含量的纳米TiO2,并研究了其光催化分解水制氢的活性。结果表明,未沉积Pt时,纯锐钛矿TiO2活性最高,金红石相含量的增加导致TiO2活性下降并在下降过程中出现波动。沉积Pt后,TiO2产氢速率随金红石相含量的增加先增后减,在金红石相质量分数为9.7%时达到最高,为40.35 mmol/(gcat.h)。首次提出了金红石相含量对混晶纳米TiO2光催化分解水制氢活性的影响机理。A series of composite-crystal nano-TiO2 powder with various contents of rutile were synthesized by the sol-gel method,and were calcined at various temperatures.The effect of rutile content on photocatalytic water-splitting for hydrogen production over composite-crystal nano-TiO2 was studied in the presence of formaldehyde as sacrificial reagent.The results showed that TiO2 with pure anatase showed the best photocatalytic activity,and increase of rutile content caused a decrease of activity,during which a fluctuation was observed.As Pt was deposited on nano-TiO2 surface by the photo-reduction method,the rates of H2 evolution increased first and then decreased with the increase of rutile content.The maximum value,which reached 40.35 mmol/(gcat.h),was obtained with nano-TiO2 containing 9.7% rutile.A mechanism of the effect of rutile content on photocatalytic water-splitting for hydrogen production over composite-crystal nano-TiO2 was proposed

Antitumor effects of a novel chloroquinoline derivative as EGFR inhibitor on human esophageal carcinoma

目的:评价Egfr抑制剂4-取代-7氯喹啉衍生物(TW9183)的体内外抗肿瘤生物活性。方法:采用分子对接技术探讨其与Egfr之间的相互作用模式,蛋白免疫印迹法评价在蛋白水平的直接作用,体外细胞生物学及体内裸鼠移植瘤模型探讨TW9183的活性。结果:Egfr受体的氨基酸残基MET769,PrO770,lyS721可与TW9183间形成氢键,蛋白印免疫迹法表明化合物抑制Egfr的磷酸化;体外细胞实验显示TW9183有明显抑制食管癌细胞增殖、周期阻滞于g2/M期及促进凋亡的效果;裸鼠食管癌移植瘤模型验证其在体内同样具有抑制肿瘤生长促进凋亡的作用。结论:TW9183在体内外实验中显示具有潜在的抗食管癌作用。Objective: To explore the underlying mechanism of 4-substituent-7-chloroquinoline derivative(TW9183),which has a selective anti-tumor effect in vitro and in vivo.Methods: Docking simulation was performed to position compounds into the EGFR structure active site to determine a possible binding model.We also used Western blot assay to directly evaluate the interaction.Cell cultures and tumor xenografts were utilized to test the effect of TW9183.Results: TW9183 was nice bound to the EGFR with the amino hydrogens of MET769, PRO770 and LYS721,forming optimal H-bonds interaction.Western blot assay showed that the compound inhibited EGFR phosphorylation.TW9183 treatment increased typical apoptosis and the number of cells in the G2/ M phase in ECA-109 cells.In ECA-109 xenograft model in nude mice,TW9183 inhibited tumor growth and promoted apoptosis.Conclusion: TW9183 has potential antitumor activity in vitro and in vivo.泉州市科技计划重点项目(2013Z35

Concentration Protile of Etchant Measured by Microelectrode Technique in the Process of Chemical Micromachining

利用微圆盘电极技术,测定了KBr、L-胱氨酸和硫酸组成的刻蚀溶液体系中Pt电极表面电化学氧化产生的刻蚀剂Br2浓度分布,为约束刻蚀剂层技术(CELT)中刻蚀体系的选择和优化提供更直观的依据.GaAs表面CELT微加工实验证明了用微圆盘电极测得的表面刻蚀剂的浓度分布趋势与微加工实验所得到的结果一致.A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining.国家高新技术发展规划(863项目)(2002AA404170);; 福建省自然科学基金计划资助项目(E0520001

Research on the status of Amphioxus and other macrofauna resources in Xiamen waters

根据2014年8月在厦门黄厝和十八线潮下带海区,以及2014年1月-6月在厦门玩月坡潮间带获得的文昌鱼及其他大型底栖动物数据,对厦门海域文昌鱼及其他大型底栖动物资源现状进行了分析。黄厝潮下带大型底栖动物物种数、栖息密度和生物量分别为25种、80.7 Ind/M2和13.46 g/M2,十八线潮下带大型底栖动物物种数、栖息密度和生物量分别为25种、76.2 Ind/M2和6.53 g/M2。黄厝潮下带大型底栖动物丰度指数(d)平均值略低于十八线潮下带,而均匀度指数(J)和物种多样性指数(H')平均值略高于十八线潮下带。黄厝潮下带、十八线潮下带的文昌鱼栖息密度分别为51.1 Ind/M2和39.8 Ind/M2文昌鱼生物量分别为4.63 g/M2和3.68 g/M2。单因素方差分析表明,除了黄厝海区和十八线海区之间的文昌鱼体长呈显著差异,其余文昌鱼种群参数和大型底栖动物群落参数均无显著差异。文昌鱼栖息密度和生物量与底质粒径分选系数呈显著负相关。玩月坡潮间带低潮区文昌鱼及其他大型底栖动物资源贫乏。厦门海域文昌鱼的资源量与文昌鱼放流、海岸工程和采砂作业有关。The status of amphioxus and other macrofauna resources in Xiamen waters were analyzed according to the am-phioxus macrofauna samples collected in the subtidal zone in October 2014 and in the intertidal zone from January 2014 toJune 2014.The species number, density and biomass of marcofauna in Huangcuo subtidal zone were, 25, 80.7 ind/m2.and13.46 g/m2 respectively, and in 18 Line subtidal zone were 25, 76.2 ind/m2 and 6.53 g/m2 respectively.The average of theabundance index (d) of macrofauna in Huangcuo subtidal zone was slightly lower than that in 18 Line subtidal zone, butthe average of the evenness index (J) and the biodiversity index (H') was opposite.The densities of amphioxus inHuangcuo and 18 Line were 51.1 ind/m2 and 3.68 g /m2 respectively, and the biomasses of amphioxus in these two areas were4.63 g/m2 and 3.68 g /m2 respectively.The result of one-way ANOVA showed that, amphioxus length in these two areas wassignificantly different.The relationships between the density, biomass of amphioxus and sediment sorting coefficient had sig-nificant negative correlation.The resources of amphioxus and other macrofauna were scarce in the low tidal zone of Wanyuepowhich located in the eastern waters of Xiamen.The resources of amphioxus related to the amphioxus artificial releasing,coastal engineering and sand mining operations.海洋公益性行业科研专项(201305030-6); 厦门大学凌锋计划资助项

Concentration protile of etchant measured by microelectrode technique in the process of chemical micromachining

A carbon-disk microelectrode was used to investigate the surface concentration profile of etchant Br-2, which was electrogenerated on the Pt working electrode. The steady state reducing currents of Br-2 at different distances away from the Pt electode was measured. The concentration profile was estimated from the current-distance variation curves as a function of different sampling times. Experimentally determined concentration profiles are in good agreement with those estimated from the microetching results. The microelectrode technique has offered a good method to choose suitable etching solution for chemical micromachining

福建城市化发展战略博士笔谈

2003年5月,“福建省博士创业促进会”在福州举办了福建省城市发展战略“博士月谈”,与会者主要是省内高校和科研单位的专家、博士。会上,大家用书面发表意见,畅所欲言,崇论宏议。本刊精选部分意见,以飨读者。相信这些意见对福建城市化问题研究的深化将起到积极的推动作用

类泛素蛋白及其中文命名

泛素家族包括泛素及类泛素蛋白,约20种成员蛋白.近年来,泛素家族领域取得了迅猛发展,并已与生物学及医学研究的各个领域相互交叉.泛素家族介导的蛋白质降解和细胞自噬机制的发现分别于2004和2016年获得诺贝尔奖.但是,类泛素蛋白并没有统一规范的中文译名. 2018年4月9日在苏州召开的《泛素家族介导的蛋白质降解和细胞自噬》专著的编委会上,部分作者讨论了类泛素蛋白的中文命名问题,并在随后的\"泛素家族、自噬与疾病\"(Ubiquitinfamily,autophagy anddiseases)苏州会议上提出了类泛素蛋白中文翻译草案,此草案在参加该会议的国内学者及海外华人学者间取得了高度共识.冷泉港亚洲\"泛素家族、自噬与疾病\"苏州会议是由美国冷泉港实验室主办、两年一度、面向全球的英文会议.该会议在海内外华人学者中具有广泛影响,因此,参会华人学者的意见具有一定的代表性.本文介绍了10个类别的类泛素蛋白的中文命名,系统总结了它们的结构特点,并比较了参与各种类泛素化修饰的酶和它们的生物学功能.文章由45名从事该领域研究的专家合作撰写,其中包括中国工程院院士1名,相关学者4名,长江学者3名,国家杰出青年科学基金获得者18名和美国知名高校华人教授4名.他们绝大多数是参加编写即将由科学出版社出版的专著《泛素家族介导的蛋白质降解和细胞自噬》的专家