Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS

采用反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)和大气压化学电离质谱(APCI-MS)联用技术,分析赤芝中含有的三萜类化合物。使用反相C18色谱柱,以乙腈和水(含2%冰醋酸)为流动相梯度洗脱,流速为0.8ml/min,室温下,色谱检测波长253nm;用DAD检测器记录各个色谱峰的紫外吸收光谱,采用APCI-MS进行在线同步分析,记录TIC总离子流色谱图和各个色谱峰的质谱图,通过紫外光谱及质谱分析并与文献对照初步鉴定了赤芝中的32个三萜类成分。High performance liquid chromatography (HPLC)-atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) was applied to analyze the triterpenoids in extracts of Ganoderma lucidum. The extraction conditions are: Hypersil C4 (4.6 mm × 150 mm) column with gradient elution with acetonitrile and water (2.0% acetic acid) as mobile phase, flow-rate 0.8 ml/min, and room temperature about 25 ℃. The detection wavelength is 253 nm and the UV spectra of peaks are obtainedwith a photodiode array detector (DAD). On-line APCI-MS in positive and negative mode was used to get the mass spectrum of the analyte. Thirty-two components of Ganoderma lucidum are primarily identified by comparison of the UV spectra and mass spectrum according to published literatures.青岛“2004将才计划”项目(04-3-JJ-11);; 共建生物医药研发测试中心资助项目(LS-05-KJZX-76

STUDY ON ELEMENTAL CONCENTRATION IN AIR PARTICULATE MATTER BY ICP-MS

This study conducted air particulate matter sampling to measure the total suspended particulate (TSP), PM 10 (particles<10 μmm in aerodynamic diameter) and PM 2 5 (particles<2 5 μmm in aerodynamic diameter) mass concentration at Tongan district in Xiamen. One hundred and nineteen samples were obtained to measure the mass concentrations of TSP, PM 10 and PM 2 5 during 15 April to 6 June 2002. According to these results, the TSP, PM 10 and PM 2 5 mass concentrations varied from 53 μg·m -3 (from 26 to 105 μg·m -3 ), 48 μg·m -3 (from 23 to 74 μg·m -3 ) and 41 μg·m -3 (from 18 to 62 μg·m -3 ), respectively. 18 elemental concentrations were determined by ICP MS, most elemental profiles were lower than previous studies. However, high levels of Pb and Zn indicate some pollutants existed in air particulate matter.SupportedbyYouthInnovationFoundationofFujian ( 2 0 0 1J0 5 3

Enhance Teaching Ouality of Chemical Engineering Thermodynamics

该文针对化工热力学课程内容深奥抽象,计算繁琐等特点,在教学过程中调整教材内容突出重点,改进教学方法,理论联系实际,加强学科间的渗透,这些做法激发了学生的学习兴趣,深化了教学内容,使学生在获得扎实的专业知识的基础上分析、解决问题的能力也得到了提高,提高了教学水平和质量.This paper analysis the characteristics of the course chemical engineering thermodynamics and problems in learning this course. Combined with the authors＇own teaching experience, some teaching methods are put forward in this paper. These methods are regarded as the effective means in improving the teaching results of this course

New Attempts about Teaching Reform for the Course: "Chemical Separate Engineering"

针对化工分离工程教学中存在的问题,提出了课程教学改革的一些新尝试.围绕培养工程复合型人才的要求,在教学过程中引入科研和实践教学环节的内容,把理论联系实际的思想贯穿于整个课程的教学中,针对学生情况,变客为主,点化悟性,激发了学生的学习兴趣,启迪和调动了学生的能动思维,深化了教学内容,收到了良好的教学效果

Study on the Teaching Practice of Chemical Reaction Engineering

根据化学反应工程课程内容的特点,在教学过程中把握课程结构,注重教学方法研究,注重理论联系实际,激发学生的学习兴趣,培养学生独立分析和解决问题的能力,从而提高学生工程分析能力.Based on character of the curricular content of chemical reaction engineering, we grasped the course structurea and pay attention to studying teaching mean and integration of theory with practices on teaching, we can stimulate student＇s leavning interest, train student＇s ability of analying and solving problems independently, and improve student＇s ability of engineering analysis

采用多种措施提高《化工原理》教学效果

针对目前化工原理课程教学中存在的问题,通过采用提高教学趣味性,加强工程观念,运用多媒体辅助教学等措施提高学生学习积极性,改善教学效果

发酵工程教学中学生模型化思维的形成

随着现代科学的发展，科学研究已深入到微观和字观领域．这就使得科学认识主体越来越难以直接研究客观对象。而必须求助于一些“中间手段”．目前，科学界所高度重视的数学建模方法，为探索微观和字观领域自然规律提供了一个行之有效的研究手段．随着模型在现代科学认识中的广泛使用，“模型化”思维业已成为现代科学认识中的一种极有价值的认识手段和思雏方式．文章拟从科学认识论的角度阐述发酵工程教学中培养学生“模型化”的创新性思维

GC Analysis of the Synthese System of Dimethyl Carbonate

利用气相色谱内标法,以正丁醇为内标,快速定量分析二氧化碳和甲醇直接合成碳酸二甲酯体系中甲醇和碳酸二甲酯,考察了柱温、载气流量对分离度的影响,确定了适宜的分析条件.该法灵敏,准确,重现性好.Applying internal standard method with normal butanol as internal substance the contents of dimethyl carbonate and methanol in the product of direct syntheses of dimethyl carbonate ware analyzed by GC with the quartz capillary column. The effects of various condition , such as column temperature , flow rates of carrier gas , and stationary phase, on the resolution were investigated. The optimal conditions for GC analysis were confirmed. The method was sensitive, accurate and reproducible.广西科技厅资助项目(桂科青0640018

Progress in down-stream processing of biologically produced 1,3-propanediol

1,3-丙二醇是一种重要的化工原料,以甘油或葡萄糖为原料发酵法制备1,3-丙二醇具有原料可再生、反应条件温和等优点,是近年来国内外的研究热点。由微生物发酵获得的1,3-丙二醇发酵液是含多种强极性的醇及盐类的稀溶液,这使得采用传统的分离方法难以经济、有效地的将1,3-丙二醇从发酵液中纯化出来,后提取过程成为发酵法工业化生产1,3-丙二醇的瓶颈。1,3-丙二醇后提取过程主要包括微生物菌体等高分子物质的去除,盐的去除、回收,有机物的纯化和水的去除。以下对应用于以上分离过程的技术的研究进展进行讨论,提出在该领域应该重视的发展方向。1,3-propanediol is an important raw material in chemical industry.Microbial conversion of glycerol or glucose into 1,3-propanediol has been given much attention due to its renewable resource,mild reaction conditions,and other advantages.It is a challenge to efficiently separate 1,3-propanediol from a mixture of multiple components,such as 1,3-propanediol,2,3-butanediol,water,residual glycerol,ethanol,macromolecules and salts,for the reason that 1,3-propanediol,glycerol and 2,3-butanediol are all very hydrophilic and have intense polarity.The conventional evaporation and distillation techniques used in the purification of 1,3-propanediol suffer from the problem of high energy consumption and low recovery.It makes the recovery of 1,3-propanediol from a fermentation broth become a bottleneck in industrial production.The down-stream processing of biologically produced 1,3-propanediol mainly includes the removal of protein,salts,water and other impurities.This paper analyze the research progress of these separation technologies and point out the developing direction worth further investigation.国家高技术研究发展计划(863计划)(No.2008AA05Z302

APPLICATION OF THE CATION EXANGE RESIN MODIFIED BY THE METAL IONS IN THE ESTERIFICATIAN

将催化精馏中常用作催化填料的强酸性阳离子交换树脂用金属离子负载修饰后,考察了树脂催化性能的改变及树脂结构对其催化性能的影响。实验表明,经修饰后树脂的催化能力都高于原树脂,在不分水的情况下,合成乙酸乙酯时乙酸的转化率最高可达73%,具有很好的选择性,金属离子能与树脂的磺酸基团产生络合,提高了树脂的催化性能,所形成的新酸中心不会被阳离子交换而失活。广西民族学院青年科研基金资助项