止痢合剂的体外抗菌作用研究

止痢合剂的体外抗菌作用研究游兰英（厦门市医药研究所厦门361003）刘广发（厦门大学生物学系厦门361005）止痢合剂是在民间验方基础上由铁苋菜和水蓼组方的纯中药制剂，具清热解毒、止泻镇痛等功效。临床上治疗急慢性胃肠炎、痢疾、腹痛腹泻等症效果甚佳。现..

Raise of the Frequency in Green-shoot-formation for Rice Anther Cultivation

福建省水稻攻关课题基金 (K790 81);; 厦门市科委科技发展项目 ( 3 5 0 2 2 2 0 0 0 10 4

Annual change of outdoor seawater cultivation of dunaliella for biomass energy

作者简介：陈昱（1982-），男，硕士，主要从事生物质能源研究。E-mail：[email protected][中文文摘]将涨潮海水浓缩后以次氯酸钠进行消毒,添加少许氮、磷等无机盐,在室外周年养殖杜氏藻及其多个突变株,研究其在自然条件下生物量的产出和细胞内脂类的积累。结果表明,在养殖过程中,培养液的盐度、pH值以及细菌含量的变化对杜氏藻的生长没有显著影响。通过培养,不同株系杜氏藻都可以获得较高的生物量和脂类产出,特别是耐高温突变株、高脂突变株可以适应夏天室外的高温、高盐等恶劣环境,普通株在冬天尚能生长,不同特性的藻株的轮换养殖可以保证养殖场周年养殖。海水养殖有望成为一种大规模培养杜氏藻的既简单又经济的发展模式。文章为进一步开发利用杜氏藻生物质能源奠定了一定的基础。[英文文摘]Dunaliella bardawil and several mutants were cultured outdoor in concentrated seawater treated by Javel water with the addition of few nitrate and phosphate,and the biomass and lipid accumulation of Dunaliella were assessed.Dunaliella species grew well during the cultivation period,despite of the change of salinity,pH and bacteria content in seawater.High lipid and biomass productivity of Dunaliella species was obtained in our study.High temperature tolerance mutant and high lipid content mutant grew well under high temperature and high salinity of seawater in summer, while normal strain grew well in winter. Alternate cultivation of different Dunaliella strain should be carried out in all-year outdoor cultivation. It would be a simple, economic batch mode for large-scale Dunaliella cultivation using concentrated seawater, and it would contribute to further using of Dunaliella for biomass energy.福建省科技重点项目(2009N0052); 福建省重大专项前期研究项目(2005YZ1022

Brown tide:A new ecosystem disruptive algal bloom

AurEOCOCCuS AnOPHAgEffErEnS和AurEOuMbrA lAgunEnSIS隶属于棕鞭藻门(OCHrOPHyTA)、海金藻纲(PElAgOPHyCEAE),在美国及南非的一些河口形成生态系统破坏性褐潮(brOWn TIdE)已经有20多年了,近年来在中国河北沿海发生的大规模褐潮也使中国成为世界上第3个受褐潮影响的国家。褐潮藻能够利用多种有机营养,在低光照及低营养条件下达到高生长速率,对贝类养殖产业、经济以及娱乐产业等造成严重的负面影响。本文总结了20多年来褐潮在全球的发生及其所造成的严重危害,对褐潮藻的形态结构、生理特征及分子遗传学,特别是促进褐潮形成及持续的关键蛋白的编码基因等研究作了简要介绍,并概括了国内褐潮的研究现状。在此基础上,展望今后褐潮的研究方向,以期为褐潮的研究、预警预报和生态学防治及其防灾减灾提供借鉴。The pelagophytes Aureococcus anophagefferens and Aureoumbra lagunensis have formed ecosystem disruptive algal blooms in shallow estuaries of the United States and South Africa for more than two decades.The large-scale brown tide events in the coastal waters of Hebei Province in recent years make China the third country that is affected by brown tide in the world.These algae are able to utilize a wide variety of organic nutrients and achieve high growth rates at low light and nutrient levels.They have significant negative impacts on the shellfish mariculture industry,economy and recreational industry.We review research progress in the last two decades on morphology,physiology and molecular and genomic characteristics of these brown tide algae,especially the genes that encode many of the key proteins that facilitate bloom formation and persistence.Moreover,the research progress in China about brown tide is summarized.The prospects of the research on brown tide are also discussed.海洋公益性行业科研专项(201205031-03和201005015-5); 国家基础研究发展计划项目(2010CB428704)资

Effect of Different Concentrations of NaCl and Light Intensities on β-carotene Content in Dunalie

以2种杜氏藻即巴氏杜氏藻和盐生杜氏藻为实验材料,在不同NaCl胁迫和光照(紫外线和高光照强度)进行培养的结果表明,细胞生长的最适盐度是2.0mol·L-1,高产β-胡萝卜素的最适盐度是3.5mol·L-1;紫外线下诱导的藻株环境适应能力较强,β-胡萝卜素含量较高;高光照强度(1080μmol·m-2·s-1)下诱导的杜氏藻β-胡萝卜素含量高;二步法培养的β-胡萝卜素含量比正常培养的提高2倍以上。福建省科技计划重点项目(2003N053

Comparison of gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen

目的比较15个短串联重复序列(STR)基因座基因频率在原发性胃腺癌患者和厦门地区正常人群中的分布,推测与胃腺癌相关的基因。方法123份血样采自本地区无癌家族史的健康人群,39份血样采自本地区胃腺癌患者。用聚合酶链反应(PCR)复合扩增结合四色荧光检测方法对血样DNA进行基因型分析,调查本地区健康人群和胃腺癌患者人群的基因频率分布,并根据两者的该15个基因座等位基因频率分布的差异性,推测易感连锁和抗性连锁的等位基因。结果厦门地区胃腺癌患者的TH01、vWA和FAG基因座的等位基因的分布与该地区健康人群比较差异有统计学意义(P<0·01)。在个别等位基因比较中,胃腺癌人群TH01-7的基因频率为0·0385,健康人群TH01-7的基因频率为0·2642,两者差异有统计学意义(P<0·01),相对危险度(RR)=0·1115;胃腺癌人群vWA-15基因频率0·0513,健康人群vWA-15的基因频率0·2927,两者差异有统计学意义(P<0·01),RR=0·1307;胃腺癌人群FAG-18的基因频率为0·1026,健康人群FAG-18的基因频率为0·0163,两者差异有统计学意义(P<0·01),RR=6·8998。结论TH01-7与胃腺癌相关联,其附近可能存在胃腺癌抗性基因;vWA-15附近有可能存在与胃腺癌相关的抗性基因;FAG-18与胃腺癌相关联,其附近可能存在胃腺癌易感基因。Objective To compare the gene frequencies of 15 STR loci between patients with primary gastric adencarcinomas and the unrelated locals from Xiamen in order to search for the genes correlated to the gastric adencarcinomas.Methods The control group consisted of 123 unrelated locals and the testing group was composed of 39 gastric adencarcinomas suffers. All genotypes of the sample DNA were analyzed by gene scan technology and multiplex PCR method with 4-colored fluorescence-labeled primers. All the polymorphic alleles of these 15 STR loci in unrelated healthy locals and patients with primary gastric adencarcinomas had been investigated. The sensitive or resistant genetic factors were inferred according to the statistical difference with distribution of allele frequencies.Results It showed that there were statistic differences (P<0.01)between controls and testing groups in allele frequencies of the three loci: TH01, vWA and FAG. The further exploration of the separated locus revealed that the gene frequency of TH01-7 in the gastric adencarcinomas suffers was 0.0385,but 0.2642 in the control group[P<0.01 and relative risk(RR)=0.1115];the gene frequency of vWA-15 in the gastric adencarcinomas suffers was 0.0513,but 0.2927 in the control group(P<0.01 and RR=0.1307);the gene frequency of FAG-18 in the gastric adencarcinomas suffers was 0.1026,but 0.0163 in the control group(P<0.01 and RR= 6.8998). Conclusions It is very possible that TH01 alleles may be associated with gastric adencarcinomas and it is possible that there is a resistant gene to gastric adencarcinomas near the region of TH01-7 locus; there is a resistant gene of gastric adencarcinomas near the region of vWA-15 locus; FAG alleles may be associated with gastric adencarcinomas and perhaps there is a sensitive gene of gastric adencarcinomas near FAG-18 locus

Cloning and Characterization of Na~+/H~+ Antiporter Gene (nhaA) from Pseudomonas sp.cn4902

根据3种生物的Na+/H+逆向转运蛋白基因(nhaA)的两端序列设计引物,利用PCR从假单胞菌 (Pseudomonassp.cn4902)中克隆得到一结构基因。该基因长1089bp,编码362个氨基酸,与E.coliK12的 nhaA基因的同源性高达97.0%。将该结构基因与pBV220构建成重组载体pBVA。SDS PAGE电泳表明:含 pBVA的转化子产生较高浓度的分子量约为41kD的蛋白,与预期相符。在含NaCl1.0mol/L的培养基中生长达 到平衡期时,转化子的菌浓度约是对照的2.3倍。经原子吸收光谱测定,转化子细胞质中Na+浓度仅为对照菌的 60.4%。SDS PAGE电泳表明该基因的表达蛋白位于细胞膜(壁)上。提纯外源基因表达蛋白并对其N端8个氨 基酸进行测序,与nhaA基因推测的氨基酸序列完全相符。这些实验证实,克隆得到的基因是假单胞菌的nhaA基 因。该基因已经在GenBank登记,收录号为AY643494。 【英文摘要】 According to the sequences of the gene nhaA coding for Na~+/H~+ antiporter,a structural gene was cloned from Pseudomonas sp.cn4902 by PCR reaction with a set of primers.It was 1 089 bp in length and codes for 362 amino acids sharing homology with the gene nhaA of E.coli K12 as high as 97.0%.It was inserted into plasmid pBV220 to form a high level expression reconstruction plasmid pBVA.So an overexpression 41 kD protein band could be found in the lane of transformant harbored with pBVA after SDS-PAGE electro...福建省科技计划重点资助项目(编号:2003N053);; 厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室项目(编号:2004106)~

Cloning of Halo-tolerant-Related Gene(s) from Halobacterium halobium

将盐生盐杆菌总 DNA的 Bam HI不完全酶切片段与质粒 p U C19连接成重组质粒 ,并以重组质粒转化 E. coli JM10 1.经 X- Gal- IPTG法筛选白色重组子 ,结合高盐平板检测 ,已经获得 3株比原受体菌的耐盐性提高 30 %～ 67%的转化子 .重组质粒酶切电泳分析表明 ,被克隆的盐生盐杆菌含耐盐相关基因的 DNA片段长度在 1.0～ 3.5kb之间 .Three recombinant plasmids which have been reconstructed with plasmid pUC19 and random DNA fragments of Halobacterium halobium were transferred to E. coli JM101. As the result, the tolerant level against to NaCl of these bacteria transferred has been raised from 0.9 to 1.2~1.5 mol·L -1 respectively. The molecular weight of these DNA fragments inserted are 1.0 , 1.8 and 3.5 kb according to the evidents of agarose eletrophoresis. So it could be referred that these three DNA fragements may contain halo tolerant related gene(s).福建省自然科学基金!资助项目 ( C960 0 4

UV Radiation and Screen for β-carotene-rich Strain of Dunaliella

以本实验室保存的7种(13株)杜氏藻为材料,筛选出β-胡萝卜素含量较高的藻株Dunaliella.bardawil(1-7)进行紫外线诱变.以橙-红色藻落为初筛标志,再经高盐、强光、低氮诱导,检测其β-胡萝卜素含量,即复筛.发现突变株11-1具有高产β-胡萝卜素的能力,其β-胡萝卜素含量高达干质量的9.48%,显著高于原出发株.此外11-1的细胞体积是原出发株的5倍多,有利于养殖和收集.The strains containing high content of β-carotene were obtained after the detection of 7 species(13 strains) of Dunaliella deposited in our laboratory.One of the strains was selected and radiated by UV.Experiment results demonstrated that the orange-red colonies of algae could be used as the preliminary marker for screening β-carotene-rich cell.A mutant,dominated as 11-1,containing β-carotene as high as 9.48%(dried mass) was selected after a serious of high salt concentration,high light intensity cultivation and lower nitrogen source conduction.Meanwhile the size of mutant 11-1 was 5 times more than its original pattern.It was propitious to cultivation and collection of the β-carotene hyper-producing new strains of Dunaliella.福建省重大专项前期研究项目(2005YZ1022);; 国家基础科学人才培养基金(J0630649);; 细胞生物学与肿瘤细胞工程教育部重点实验室开放基金(2005104)资