Supplementary data for article: Kop, T. J.; Dordevic, J.; Bjelakovic, M. S.; Milić, D. Fullerene Bisadduct Regioisomers Containing an Asymmetric Diamide Tether. Tetrahedron 2017, 73 (50), 7073–7078. https://doi.org/10.1016/j.tet.2017.10.069

Supplementary material for: [https://doi.org/10.1016/j.tet.2017.10.069]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2565

Electrochemical behaviour of serpentinite and forsterite in ferri/ferro cyanide benchmark redox system

Serpentinite rock collected near Kopaonik Mountain in Serbia is considered as a multiphase assemblage mostly consisting of mineral lizardite. Forsterite has been obtained by chemical-thermal treatment of grounded sample of serpentinite. Electrochemical features of these two materials were investigated by using modified carbon paste electrode (CPE) which included mixing analytes, graphite powder and parafin oil as a binder and packing the mixture in a Teflon holder. Morphological and structural characteristics of the samples were analyzed by SEM and XRD techniques which revealed presence of certain crystal phases and agregates of irregular shapes sized from 10 to 40 μm. Cyclic voltammetry was employed to study their electrochemical characteristics. The peak current height and peak-to-peak separation showed that the electrochemical reaction is quasi-reversible. Kinetic parameters were derived from Tafel analysis. Calculated kinetic parameters indicated prominent electrocatalytic effect of the minerals for ferri/ferro cyanide redox reaction in aquaeous solutionBook Of Abstracts of 4th Conference of The Serbian Society for Ceramic Material

Element concentration and fatty acid composition of Serbian bee bread

The element concentration (Cu, Fe, Zn, Mn, Cr, Co, Ni, Se, K, Na, Ca and Mg), heavy metal concentration (Cd, Hg, Pb and As) and fatty acid composition of 12 Serbian bee bread samples from different geographical origins were examined. The element concentration was examined using ICP-MS, and total lipids for fatty acid determination were extracted from homogenized bee bread samples with hexane/isopropanol mixture by accelerated solvent extraction. Potassium was the major element, ranging between 5515 +/- 361.20 mg/kg and 7487 +/- 381.50 mg/kg. The highest As and Pb concentrations were found in bee breads from Lazarevac. This bee bread also contained the highest level of PUFA and SFA. Also, the n-6/n-3 ratio ranged between 0.86 +/- 0.28 and 1.40 +/- 0.05, indicating bee bread can be a good source of unsaturated fatty acids. Bee bread could be useful in monitoring environmental contamination by heavy metals (Cd, Hg, Pb and As), although complex studies of all bee products give sufficient information on this topic

Supplementary data for article: Kop, T. J.; Dordevic, J.; Bjelakovic, M. S.; Milić, D. Fullerene Bisadduct Regioisomers Containing an Asymmetric Diamide Tether. Tetrahedron 2017, 73 (50), 7073–7078. https://doi.org/10.1016/j.tet.2017.10.069

Supplementary material for: [https://doi.org/10.1016/j.tet.2017.10.069]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2565

Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress

Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis has been implicated in the pathophysiology of depression and stress disorders. Glucocorticoids, key regulators of stress response, have diverse effects on cellular processes in the hippocampus. Beside non genomic pathways, glucocorticoids effects are mediated through activation of the glucocorticoid receptor (GR), a ligand activated transcriptional factor that belongs to the nuclear hormone receptor superfamily. We analysed the GR protein level both, in the cytoplasmic and nuclear compartments in Wistar rat hippocampus, exposed to 3 week social isolation stress upon chronic fluoxetine treatment. Under chronic stress, corticosterone level was decreased compared to the control and treatment with fluoxetine did not change its level significantly in stressed animals. At the molecular level, fluoxetine significantly decreased the level of nuclear GR protein in the brain hippocampus of the chronically stressed rats. Fluoxetine reversed the nuclear level of GR disrupted by chronic psychosocial isolation (CPSI), but it failed to normalize HPA axis activity

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats

Identification and Perceived Value of Ecosystem Services of Urban Green Areas. Case Study: Kamenički Park in Novi Sad, Serbia

Urban parks, as one of the most significant urban green areas (UGA), bring many direct or indirect benefits, including but not limited to ecosystem services (ES) (Mexia et al. 2018). These services are often generalized, unquantified and poorly supported by empirical evidence and their negative consequences – disservices are rarely mentioned (Roman et al., 2021; Pataki et al., 2011). In order to improve planning, design and management of UGA that can increase their environmental benefits (Xie et al. 2019), better understanding and additional field research of ES and disservices is required. As a result of a survey of employees of the Institute for Nature Conservation of Vojvodina Province that manages the park and on-site users, this study represents a research of the perceived value of generated ES on the example of Kamenički park in Novi Sad, as there are no studies on the actual value of those ES. After identifying major ES, the employees and users evaluated them by their perception on a scale of 1 – the ES is well provided, 2 – service is provided but can be enhanced and 3 – the service is not provided, but has potential. Employees mainly emphasized regulation and supporting services (regulation of air quality, noise reduction, wind protection, maintaining biodiversity), but also cultural services (educational role, recreation and enjoying nature, spiritual peace and prosperity). Users opted mainly for cultural services (recreation and enjoying nature and inspiration for culture, art and design), but also for supporting services like maintaining biodiversity. The specific location, valuable natural and cultural-historical characteristics of Kamenički park contribute to the provision of numerous ES, but also offer the opportunity to develop additional ones that are currently not represented or are not sufficiently provided. The opinions and needs of stakeholders and users regarding ES can be a guideline in planning in accordance with the ES, which is a step to sustainable cities. Quantifying the degree to which various ES are related to plant diversity and structure would provide evidence of the ability to manipulate designed ecosystems to maximize the benefits they provide to urban landscapes (Nighswander et al. 2021)

Butler-volmer current equation and fractal nature correction in electrochemical energy

The Global Energy Crisis necessitated improving research into new, renewable and alternative energy sources. Due to that, our focus is on the area of some phenomena and applications where different synthetic methods and microstructure property optimization achieved significant improvement in the electro physical properties of output materials and components. This is especially important for higher energy efficiency and electricity production (batteries and battery systems, fuel cells, and hydrogen energy). The improvement of energy storage tank capacity is one of the most important development issues in the energy sphere too. It is because of this very promising research and application area that we are expanding the knowledge on these phenomena through fractal nature analysis. So, the results obtained in the field of electrochemical energy sources, especially in electrolyte development, are taken into account the analysis of fractal nature optimization. Based on the research field of fractal material science, particularly electronic materials, we conducted research in micro-structure fractal influence in the area of electrochemistry. We investigated the consolidation parameters of Fe2O3 redox processes. The influence of activation energy, fundamental thermodynamic parameters, and also the fractal correction of electrode surface area through complex fractal dimension with recognized grains and pores, and the Brownian motion of particles is introduced. Finally, the electrochemical Butler-Volmer equation fractalization is obtained. These results practically open new frontiers in electrochemical energy processes performed through the Arrhenius equation within electrolyte bulk and electrode relations and more complete and precise energy generation

Sinteza i farmakološko ispitivanje (±)-2,3-seco-analoga fentanila

An efficient, five-step synthetic approach to various acyclic 1,3-diamines has been developed and applied to the preparation of a novel class of open-chained fentanyl analogues. The acyclic derivatives 5.1-5.5 (all new compounds) were synthesized with the aim of estimating the significance of the piperidine ring for the opioid analgesic activity of anilido-piperidines. The starting beta-keto-amide 1.1, prepared by the aminolysis of methyl acetoacetate with methyl phenethylamine, (93% yield), was successively reacted with NaH and BuLi, to form the highly reactive alpha,gamma-dienolate anion 1.1a. Regio and chemoselective gamma-alkylation of the dienolate with various primary and secondary alkyl halides furnished the beta-keto-amides 1.2-1.5 (76-91%). Reductive amination of the keto-amides 1.1-1.5 with aniline and Zn powder in acetic acid, via the enamine intermediates 2.1-2.5. afforded the beta-anilino amides 3.1-3.5 (74-85%). After reductive deoxygenation of the tertiary amide group, using in situ generated diborane, the corresponding 1,3-diamines 4.1-4.5 were obtained (87-97%). The synthesis of (+/-)-2,3-seco-fentanyls 5.1-5.5 was completed by N-acylation of the diamines 4.1-4.5 with propionyl chloride, followed by precipitation of the monooxalate salts (86-95%). The parent compound, 2,3-seco-fentanyl 5.1, was found to be a 40 times less potent narcotic analgesic than fentanyl but still 5-6 times more active than morphine in rats. while i-Pr derivative 5.3 was inactive. Apart from the pharmacological significance. the general procedure described herein may afford various functionalized, 1,3-diamines as potential complexing agents and building blocks for the synthesis of aza-crown ethers.Razvijen je efikasan postupak za dobijanje različitih acikličnih 1,3-diamina u pet faza, i primenjen u sintezi nove klase analoga fentanila otvorenog niza. Derivati 5.1–5.5 (svi su nova jedinjenja) sintetisani su sa ciljem da se proceni uticaj piperidinskog prstena na opioidnoanalgetičku aktivnost anilido-piperidina. Polazni β-keto-amid 1.1, dobijen aminolizom metilacetoacetata metilfenetilaminom (prinos 93 %), bio je sukcesivno tretiran sa NaH i BuLi, pri čemu je postao veoma reaktivni α,γ-dienolatni anjon 1.1a. Regio- i hemoselektivnim γ-alkilovanjem ovog dienolata različitim primarnim i sekundarnim alkil-halogenidima, dobijeni su β-keto-amidi 1.2–1.5 (prinos 76–91 %). Reduktivnim aminovanjem keto-amida 1.1–1.5 pomoću Zn praha i sirćetne kiseline, preko enaminskih intermedijera 2.1–2.5, postali su -anilino-amidi 3.1–3.5 (prinos 74–85 %). Posle reduktivne deoksigenacije tercijene amidne funkcije, koristeći in situ generisani diboran, odgovarajući 1,3-diamini 4.1–4.5 izolovani su u prinosima 87–97 %. Sinteza (±)-2,3-seco-fentanila 5.1–5.5 završena je N-acilovanjem diamina 4.1–4.5 propionil-hloridom, a zatim taloženjem u obliku monooksalatnih soli (prinos 86–95 %). Nađeno je da je osnovno jedinjenje, 2,3-seco fentanil 5.1, 40 puta slabiji narkotički analgetik od fentanila, ali još uvek 5–6 puta aktivniji od morfina u pacova, dok je i-Pr derivat 5.3 bio neaktivan. Osim farmakološkog značaja, opštim postupkom prikazanim u ovom radu, mogu se sintetisati različiti 1,3-diamini, uključujući i one sa funkcionalnim grupama. Ova jedinjenja mogu biti potencijalno značajna kao kompleksirajući agensi i kao intermedijeri u sintezi aza-kraun-etara

Effects of cisplatin on lipid peroxidation and the glutathione redox status in the liver of male rats: The protective role of selenium

The role of oxidative stress in cisplatin (CP) toxicity and its prevention by pretreatment with selenium (Se) was investigated. Male Wistar albino rats were injected with a single dose of cisplatin (7.5 mg CP/kg b.m., i.p.) and selenium (6 mg Se/kg b.m, as Na2SeO3, i.p.) alone or in combination. The results suggest that CP intoxication induces oxidative stress and alters the glutathione redox status: reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio (GSH RI), resulting in increased lipid peroxidation (LPO) in rat liver. The pretreatment with selenium prior to CP treatment showed a protective effect against the toxic influence of CP on peroxidation of the membrane lipids and an altering of the glutathione redox status in the liver of rats. From our results we conclude that selenium functions as a potent antioxidant and suggest that it can control CP-induced hepatotoxicity in rats.U našoj studiji ispitivana je uloga oksidacionog stresa u toksičnosti cisplatina (SR) i njegova prevencija pretretmanom selenom (Se). Mužjaci Wistar albino pacova su inicirani jednom dozom cisplatina (7.5 mg CP/kg t.m., i.p.) i selena (6 mg Se/kg t.m, kao Na2SeO3, i.p.) pojedinačno ili u kombinaciji. Rezultati pokazuju da intoksikacija SR uzrokuje oksidacioni stres i promenu glutation redoks statusa: redukovanog (GSH), oksidovanog (GSSG) i GSH/GSSG indeksa (GSH RI), kao i povećanje lipidne peroksidacije (LPO) u jetri pacova. Tretman Se koji je prethodio tretmanu SR pokazao je zaštitne efekte protiv toksičnog delovanja SR na peroksidaciju lipida membrane i promenu glutation redoks statusa u jetri pacova. Na osnovu naših rezultata zaključujemo da Se, deluje kao snažan antioksidans i da može imati ulogu u kontroli SR indukovane hepatotoksičnosti kod pacova.Projekat ministarstva br. 143035