Quality control of dental adhesive system in an “in vitro” experimental model of human gingival fibroblasts

En este trabajo se evaluó la viabilidad de fibroblastos gingivales humanos en contacto con distintos sistemas adhesivos dentales mediante la utilización del método de cuantificación de LDH libre y de un análisis morfológico. Se utilizaron fibroblastos gingivales humanos cultivados en placas de 24 pocillos a una concentración de 20000 células/500 ìl de medio de cultivo DMEM con 10% FBS y antibióticos. Los materiales evaluados fueron: Adper SE Plus (ADSE), Adper Single Bond (SB) y One Coat (OC). A las 24 horas se realizó la cuantificación de la liberación de la enzima lactato deshidrogenasa (LDH) en un lector de placas ELX-800. Para el análisis morfológico las células adheridas en los pocillos fueron observadas con un microscopio óptico de luz invertida Nikon Japan Optifhot-2. Las células controles no recibieron tratamiento. Al evaluar LDH los valores fueron: ADSE 42,1%, SB 46,5%, OC 39,6%. A nivel morfológico las células en contacto con los distintos materiales usados en la experiencia mostraron alteraciones caracterizadas por células que toman formas esféricas y que presentan ruptura de sus membranas plasmáticas. En conclusión, los distintos sistemas adhesivos dentales utilizados generarían alteraciones irreversibles en la viabilidad celular de los fibroblastos gingivales humanos por la ruptura de sus membranas plasmáticas.The objetive of this study was to evaluate human gingival fibroblast viability in contact with different dental adhesive systems by means of both free LDH quantification method and morphological analyses. Human gingival fibroblast grow to in 24 wells plates to 20.000cells/500ul concentration DMEM culture medium whith 10% and antibiotics were used. Materials assessed were: Adper SE Plus (ADSE), Adper Single Bond (SB), One Coat (OC). After 24 hours deshidrogenasa lactate enzyme releasing quantification was performed. The analysis was carried out through ELX- 800 (Biotek) plates reader. For morphologic analysis cells adhered in the wells were observed by light microscopy Nikon Japan Optifhot-2. Control cells were not treated. When evaluating LDH, results were: ADSE 42,1%, SB 46,5%, OC 39,6%. At morphogical level cells in contact to different experimental materials showed some alterations characterized by cells which become spherical and present plasmatic membrane disruption. These results allow us to conclude that different dental adhesive systems used do creat or posibility cellular viability irreversible alterations in human gingival fibroblast due to plasmatic membrane disruption

Análisis de la interface diente-material restaurador, utilizando cementos encapsulados bioactivos

Objetivos Evaluar mediante observación con microscopía LASER Confocal las interfaces diente-material de tres cementos para restauración posterior en presentación de cápsulas pre-dosificadas. Métodos Se confeccionaron 18 cavidades de Clase 1, oclusales simples de 4mm x 4mm x 4mm, en el centro de la cara oclusal de terceros molares sanos, extraídos recientemente por razones ortodóncicas, éstas fueron obturadas con tres cementos para restauración basados en cementos de ionómero de vidrio, siguiendo las instrucciones del fabricante, determinando tres grupos de tratamiento:-Grupo 1:Sistema EQUIA (GC America, Chicago, US); -Grupo 2:Chemfil Rock (Denstply/De Trey, Konstanz, GE;Grupo 3:GlassCarbomer Fil (GCP Dental, Verhein, NED. Las muestras fueron observadas en el Microscopio Confocal Biológico Invertido de Barrido mediante laseres modelo LSM5Pascal ZeissGermany. El software estadístico utilizado fue el SPSS v.15.Se midieron 10 puntos de la pared pulpar. Las mediciones fueron realizadas con el programa informático AXIO-Vs 40 V 4.8.2.0 Carl ZeissMicroImaginGmbH. RESULTADOS Las interfaces dentina profunda de la pared pulpar fue: EQUIA 0,47um, Chemfil Rock 0,6um,Carbomer GCP: 2,25um CONCLUSIONES La interface mayor correspondió al Carbomero, seguido de Chemfil y las minimas fue EQUIA.http://www.suio.com.uy/suio/wp-content/uploads/2013/06/PROGRAMA-VIII-SUIO.pdfFil: Rozas, Carlos Alfredo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Operatoria I A; Argentina.Fil: Rodriguez, Ismael Ángel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Histología y Embriología B; ArgentinaFil: Molina, Gustavo Fabían. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Materiales Dentales; Argentina.Fil: Hernando, Luis Mario. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Odontología Legal; Argentina.Fil: Uribe Echevarria, Leonardo. Universidad Nacional de Córdoba. Cátedra de Operatoria Dental; Argentina.Odontología, Medicina y Cirugía Ora

Toward Pricing Financial Derivatives with an IBM Quantum Computer

Pricing interest-rate financial derivatives is a major problem in finance, in which it is crucial to accurately reproduce the time evolution of interest rates. Several stochastic dynamics have been proposed in the literature to model either the instantaneous interest rate or the instantaneous forward rate. A successful approach to model the latter is the celebrated Heath-Jarrow-Morton framework, in which its dynamics is entirely specified by volatility factors. In its multifactor version, this model considers several noisy components to capture at best the dynamics of several time-maturing forward rates. However, as no general analytical solution is available, there is a trade-off between the number of noisy factors considered and the computational time to perform a numerical simulation. Here, we employ the quantum principal component analysis to reduce the number of noisy factors required to accurately simulate the time evolution of several time-maturing forward rates. The principal components are experimentally estimated with the five-qubit IBMQX2 quantum computer for 2 x 2 and 3 x 3 cross-correlation matrices, which are based on historical data for two and three time-maturing forward rates. This paper is a step towards the design of a general quantum algorithm to fully simulate on quantum computers the HeathJarrow-Morton model for pricing interest-rate financial derivatives. It shows indeed that practical applications of quantum computers in finance will be achievable in the near futureThe authors acknowledge the use of IBM QISKIT for this work. The views expressed are those of the authors and do not reflect the official policy or position of IBM. We also acknowledge funding from projects QMiCS (Grant No. 820505) and OpenSuperQ (Grant No. 820363) of the EU Flagship on Quantum Technologies; the FETOPEN project QuroMorphic, Spanish Government, Grant No. PGC2018-095113-B-I00 (MCIU/AEI/FEDER, UE); Basque Government, Grant No. IT986-16; Spanish Ramon y Cajal Grant No. RYC-2017-22482; Shanghai Municipal Science and Technology Commission (Grants No. 18010500400 and No. 18ZR1415500); and the Shanghai Program for Eastern Scholar. This work is supported by the US Department of Energy, Office of Science, Office of Advanced Scientific Computing Research (ASCR) quantum algorithm teams program, under field work Proposal No. ERKJ333. We also acknowledge support from NSFC (Grant No. 12075145), STCSM (Grants No. 2019SHZDZX01ZX04, No. 18010500400, and No. 18ZR1415500), and from Spanish Government PID2019-104002GB-C21, PID2019104002GBC22 (MCIU/AEI/FEDER, UE

Características clínicas del estado epiléptico en niños de un hospital de tercer nivel de Lima.

Status Epilepticus (SE) in children exhibits high levels of morbidity and mortality and has been poorly studied in Perú. Objective: To describe the frequency and clinical characteristics of children with Status Epilepticus treated at Cayetano Heredia Hospital in Lima. Material and Methods: Retrospective and observational study, case series type, in children under 14 years of age, with Status Epilepticus treated between January and December of 2021. Results: SE was recorded in 28.8% of children with epileptic seizures seen during 2021. Twenty-three children presenting 46 SEs were included. The median age was 2 years, the majority (73,9%) being males, with diagnosis of epilepsy or brain malformation (21.7%) and treated in the pediatric emergency (91%). The predominant semiological pattern was of generalized motor type (52.2%), with remote symptomatic etiology (73.9%), and EEG recorded in a 63% of all cases, mainly reported as normal (75,9%). The median duration was 22 minutes of total resolution, 7 minutes, and of partial resolution, 2 minutes. Midazolam was used as the first line of treatment (78%), and phenytoin as the second line (85%). Conclusions: SE was a frequent condition observed in children throughout the year 2021. Most affected children were male, the predominant etiology was remote symptomatic, and the most common semiological type was the generalized motor. The children were treated according to international management guidelines. A systematic epidemiological surveillance approach is highly encouraged.El estado epiléptico (EE) en niños exhibe altos niveles de morbi-mortalidad y ha sido poco estudiado en el Perú. Objetivo: Describir la frecuencia y características clínicas de niños con estado epiléptico, atendidos en el Hospital Cayetano Heredia de Lima. Material y métodos: Estudio retrospectivo y observacional, tipo serie de casos de niños menores de 14 años con diagnóstico de estado epiléptico, atendidos entre enero y diciembre de 2021. Resultados: Se registró EE en un 28,8% de todos los niños atendidos por crisis epilépticas durante el año 2021. Se incluyeron 23 niños que presentaron 46 EE, varones (73,9%), con una mediana de la edad de 2 años, diagnóstico subyacente de epilepsia o malformación cerebral (21,7%), atendidos en emergencia pediátrica (91%). El tipo semiológico predominante fue el EE motor generalizado (52,2%), con etiología sintomática remota (87%) y EEG registrado en un 63% de los casos, predominantemente de tipo normal (75,9%). La mediana de duración fue de 22 minutos, de resolución total, 7 minutos y resolución parcial, 2 minutos. Se empleó midazolam (78%) como primera línea de tratamiento y fenitoína (85%), como segunda línea. Conclusiones: El EE en niños fue una emergencia prevalente durante el año 2021. La mayoría fueron varones, cuya etiología predominante fue sintomática remota y el tipo semiológico más común fue el motor generalizado. Los niños fueron atendidos de acuerdo con las guías internacionales de manejo. Se insiste en la necesidad de un sistemático afronte de vigilancia epidemiológica

The Spectrum of Interstitial Lung Disease Associated With Autoimmune Diseases: Data of a 3.6-Year Prospective Study From a Referral Center of Interstitial Lung Disease and Lung Transplantation

Interstitial lung disease (ILD) may occur in patients with a rheumatic autoimmune disease (AD), increasing their risk of morbidity and mortality. However, little is known about the prevalence of AD in patients diagnosed with an ILD. In this prospective study, we determined the spectrum of ILD associated with AD (AD-ILD) among patients sent for assessment to a single clinic of ILD and lung transplantation from a referral center between May 2016 and December 2019. ILD diagnosis was made by pneumologists based on clinical and radiological findings and pulmonary function test abnormalities. All patients with ILD were also assessed by experienced rheumatologists. During the period of assessment, 338 patients were diagnosed with ILD. Among them, 32.8% fulfilled definitions for an AD. Most cases with AD-ILD had a diagnosis of rheumatoid arthritis (27.0%), systemic sclerosis (26.1%) or anti-synthetase syndrome (17.1%). Interestingly, 18% of the patients with AD-ILD were diagnosed as having an interstitial pneumonia with autoimmune features. Antinuclear antibodies and non-specific interstitial pneumonia were the most frequent positive autoantibodies and radiological pattern found in AD-ILD patients, respectively. In conclusion, our study indicates that a high number of ILD patients have a related AD. Consequently, close collaboration among rheumatologists and pneumologists is needed.This research received no external funding. SR-M is supported by funds of the RETICS Program (RD16/0012/0009) (Instituto de Salud Carlos III, co-funded by the European Regional Development Fund)

Real world patient-reported outcomes in HIV-infected adults switching to EVIPLERA®, because of a previous intolerance to cART. PRO-STR study

To investigate the impact of switching from stable Combined Antiretroviral Therapy (cART) to single-tablet regimen (RPV/FTC/TDF=EVIPLERA®/COMPLERA®) on patient-reported outcomes in HIV-infected adults who cannot tolerate previous cART, in a real-world setting. PRO-STR is a 48-week observational, prospective, multicenter study. Presence and magnitude of symptoms (main endpoint), health-related quality-of-life (HRQoL), adherence, satisfaction with treatment and patient preferences were assessed. Three hundred patients with 48-week follow-up, who switched to EVIPLERA® (mean age: 46.6 years; male: 74.0%; 74.7% switched from a non-nucleoside reverse-transcriptase-inhibitor, 25.3% from a protease inhibitor + ritonavir) were included. There was no statistical difference in median CD4+ cell count (baseline: 678.5 cells/mm; 48-week: 683.0 cells/mm) neither in virological suppression (≤50 copies/mL) (baseline: 98.3%; 48-week: 95.3%). The most frequent reasons for switching were neuropsychiatric (62.3%), gastrointestinal (19.3%) and biochemical/metabolic (19.3%) events. Only 7.7% of patients permanently discontinued therapy. At 48-week, all outcomes showed an improvement compared to baseline. Overall, there was a significant decrease (p-value≤0.05) in number and magnitude of symptoms, while HRQoL, satisfaction and adherence improved significantly. Most patients prefered EVIPLERA® than previous cART. According to the type of intolerance, HRQoL was improved, but only significantly in patients with neuropsychiatric and gastrointestinal symptoms. Adherence improved significantly in patients with metabolic disturbances and satisfaction with EVIPLERA® was higher in the three groups. Switching to EVIPLERA® from non-nucleoside reverse-transcriptase-inhibitor or protease inhibitor-based regimens due to toxicity, improved the presence/magnitude of symptoms, HRQoL, and preference with treatment. EVIPLERA® maintained a virological response, CD4+ cell count and maintained or improved adherence

Endothelial Progenitor Cells as a Potential Biomarker in Interstitial Lung Disease Associated with Rheumatoid Arthritis

Interstitial lung disease (ILD) increases morbidity and mortality in patients with rheumatoid arthritis (RA). Although the pathogenesis of ILD associated with RA (RA-ILD+) remains poorly defined, vascular tissue is crucial in lung physiology. In this context, endothelial progenitor cells (EPC) are involved in endothelial tissue repair. However, little is known about their implication in RA-ILD+. Accordingly, we aimed to investigate the potential role of EPC related to endothelial damage in RA-ILD+. EPC quantification in peripheral blood from 80 individuals (20 RA-ILD+ patients, 25 RA-ILD? patients, 21 idiopathic pulmonary fibrosis (IPF) patients, and 14 healthy controls) was performed by flow cytometry. EPC were considered as CD34+, CD45low, CD309+ and CD133+. A significant increase in EPC frequency in RA-ILD+ patients, as well as in RA-ILD? and IPF patients, was found when compared with controls (p < 0.001, p = 0.02 and p < 0.001, respectively). RA-ILD+ patients exhibited a higher EPC frequency than the RA-ILD? ones (p = 0.003), but lower than IPF patients (p < 0.001). Our results suggest that EPC increase may represent a reparative compensatory mechanism in patients with RA-ILD+. The degree of EPC frequency may help to identify the presence of ILD in RA patients and to discriminate RA-ILD+ from IPFThis work was partially supported by the European Regional Development Fund (ERDF) and ‘Fondo de Investigación Sanitaria’ [PI18/00043] from Instituto de Salud Carlos III (ISCIII), Health Ministry, Spain. VP-C is supported by a pre-doctoral grant from IDIVAL [PREVAL 18/01]. SR-M is supported by funds of RETICS Program [RD16/0012/0009, ISCIII, co-funded by ERDF]. BA-M is a recipient of a ‘López Albo’ Post-Residency Programme funded by Servicio Cántabro de Salud. LL-G is supported by funds of ISCIII, co-funded by ERDF [PI18/00042]. OG is beneficiary of a grant funded by Xunta de Galicia, Consellería de Educación, Universidade Formación Profesional and Consellería de Economía, Emprego e Industria (GAIN), GPC IN607B2019/10. RL-M is a recipient of a Miguel Servet type I fellowship [ISCIII, co-funded by European Social Fund—ESF, CP16/00033]

Goal-Oriented p -Adaptivity using Unconventional Error Representations for a 1D Steady State Convection-Diffusion Problem

International audienceThis work proposes the use of an alternative error representation for Goal-Oriented Adaptivity (GOA) in context of steady state convection dominated diffusion problems. It introduces an arbitrary operator for the computation of the error of an alternative dual problem. From the new representation, we derive element-wise estimators to drive the adaptive algorithm. The method is applied to a one dimensional (1D) steady state convection dominated diffusion problem with homogeneous Dirichlet boundary conditions. This problem exhibits a boundary layer that produces a loss of numerical stability. The new error representation delivers sharper error bounds. When applied to a p-GOA Finite Element Method (FEM), the alternative error representation captures earlier the boundary layer, despite the existing spurious numerical oscillations