Haloglomus irregulare gen. nov., sp. nov., a New Halophilic Archaeon Isolated from a Marine Saltern

A halophilic archaeal strain, designated F16-60T, was isolated from Isla Cristina marine saltern in Huelva, Spain. Cells were pleomorphic, irregular, non-motile, and Gram-stain-negative. It produced red-pigmented colonies on agar plates. Strain F16-60T was extremely halophilic (optimum at 30% (w/v) NaCl) and neutrophilic (optimum pH 7.5). Phylogenetic tree reconstructions based on 16S rRNA and rpoB´ gene sequences revealed that strain F16-60T was distinct from species of the related genera Natronomonas, Halomarina, and Halomicrobium, of the order Halobacteriales. The polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS), and one glycolipid chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1). The DNA G+C content is 68.0 mol%. The taxonomic study, based on a combination of phylogenetic, genomic, chemotaxonomic, and phenotypic analyses, suggest that strain F16-60T (= CECT 9635T = JCM 33318T), represents a novel species of a new genus within the family Haloarculaceae and the order Halobacteriales, for which the name Haloglomus irregulare gen. nov., sp. nov. is proposed. Metagenomic fragment recruitment analysis revealed the worldwide distribution of members of this genus and suggested the existence of other closely related species to be isolated.España, MINECO project CGL2017-83385-PEspaña, Junta de Andalucía (BIO-213, US-1263771

Arteterapia en la hospitalización infantil. Interacciones entre los acompañantes y los niños hospitalizados.

Este proyecto de investigación muestra un trabajo de análisis y reflexión acerca de la práctica Arteterapéutica basada en las experiencias vividas durante ocho meses de sesiones prácticas con un colectivo de niños y adolescentes hospitalizados y las personas que les acompañan durante el periodo de hospitalización. Se han realizado talleres de Arteterapia ofreciendo un espacio y un tiempo en el que a través del juego, la ficción y la creación, los niños puedan vivenciar su estancia hospitalaria de manera más normalizada y humanizada. Se ha observado que las sesiones han promovido sentimientos positivos y han favorecido la incorporación de nuevas formas de ver y afrontar las situaciones vividas, promoviendo el bienestar y la salud del niño, integrando los diversos estados emocionales (ansiedad, incertidumbre, miedo, dolor) que forman parte de esta experiencia hospitalaria tanto en los niños como en las familias que lo acompañan. Se han analizado las interacciones producidas durante las sesiones entre las personas que lo acompañan y cuidan, que normalmente son las personas con las que ha creado un vínculo (madre, padre, abuelos…) y los niños hospitalizados. Estas interacciones en gran medida están influidas por el alto grado de estrés que crea el medio hospitalario en el que están. Este proyecto consta de cuatro partes: en la primera parte, se describe cómo se ha llevado a cabo el proceso de investigación, sus objetivos y la metodología empleada para pasar a la segunda parte en la que se establece un marco teórico, el cuál permite indagar sobre la hospitalización infantil, el impacto que genera en los niños y cómo el Arteterapia ayuda a mejorar la vivencia de esta experiencia. En la tercera parte, se desarrolla el contenido y los resultados de la investigación, a través de diferentes ejemplos y un estudio de caso. Por último, se desarrollan las conclusiones, las cuáles apuestan por el Arteterapia como ayuda en los procesos hospitalarios. Palabras clave: Arteterapia, hospitalización, juego, creación, familiares, estrés, miedo, materiales artísticos.Departamento Didáctica de la Expresión Musical, Plástica y Corporal.Máster en Arteterapia y Educación Artística para la Inclusión Socia

Análisis jurídico de la prueba testifical : régimen legal y aspectos procedimentales = Legal analysis of the testimonial evidence : the regulations and the procedural aspects

Desde que el ser humano tiene la pretensión de hacer justicia, desde el nacimiento del proceso, las partes se han valido del interrogatorio de testigos como un medio de prueba. Sin embargo, a pesar de la constante evolución en la proposición, admisión y práctica de la prueba testifical, esta sigue encarnando dificultades para los jueces y Tribunales llegado el momento de realizar su valoración. En el presente trabajo, se estudia este medio probatorio en su conjunto, partiendo de su conceptualización y la figura del testigo, el análisis de su procedimiento probatorio y finalizando con los criterios de su valoración, centrándonos en la dificultad de obtener la certeza de los hechos a través de las declaraciones testificale

Halorubrum chaoviator sp. nov., a haloarchaeon isolated from sea salt in Baja California, Mexico, Western Australia and Naxos, Greece

hree halophilic isolates, strains Halo-G*T, AUS-1 and Naxos II, were compared. Halo-G* was isolated from an evaporitic salt crystal from Baja California, Mexico, whereas AUS-1 and Naxos II were isolated from salt pools in Western Australia and the Greek island of Naxos, respectively. Halo-G*T had been exposed previously to conditions of outer space and survived 2 weeks on the Biopan facility. Chemotaxonomic and molecular comparisons suggested high similarity between the three strains. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strains clustered with Halorubrum species, showing sequence similarities of 99.2–97.1 %. The DNA–DNA hybridization values of strain Halo-G*T and strains AUS-1 and Naxos II are 73 and 75 %, respectively, indicating that they constitute a single species. The DNA relatedness between strain Halo-G*T and the type strains of 13 closely related species of the genus Halorubrum ranged from 39 to 2 %, suggesting that the three isolates constitute a different genospecies. The G+C content of the DNA of the three strains was 65.5–66.5 mol%. All three strains contained C20C20 derivatives of diethers of phosphatidylglycerol, phosphatidylglyceromethylphosphate and phosphatidylglycerolsulfate, together with a sulfated glycolipid. On the basis of these results, a novel species that includes the three strains is proposed, with the name Halorubrum chaoviator sp. nov. The type strain is strain Halo-G*T (=DSM 19316T =NCIMB 14426T =ATCC BAA-1602T)

Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea

Metagenomic studies on prokaryotic diversity of hypersaline soils from the Odiel saltmarshes, South-west Spain, revealed a high proportion of genomic sequences not related to previously cultivated taxa, that might be related to haloarchaea with a high environmental and nutritional flexibility. In this study, we used a culturomics approach in order to isolate new haloarchaeal microorganisms from these hypersaline soils. Four haloarchaeal strains, designated strains F24AT, F28, F27T, and F13T, phylogenetically related to the genus Halomicroarcula, were isolated and characterized in detail. The phylogenomic tree based on the 100 orthologous single-copy genes present in the genomes of these four strains as well as those of the type strains of the species Halomicroarcula pellucida CECT 7537T, Halomicroarcula salina JCM 18369T and Halomicroarcula limicola JCM 18640T, that were determined in this study, revealed that these four new isolates clustered on three groups, with strains F24AT and F28 within a single cluster, and altogether with the species of Halomicroarcula. Additionally, Orthologous Average Nucleotide Identity (OrthoANI), digital DNA-DNA hybridization (dDDH) and Average Amino-acid Identity (AAI) values, likewise phenotypic characteristics, including their polar lipids profiles, permitted to determine that they represent three new species, for which we propose the names Halomicroarcula rubra sp. nov. (type strain F13T), Halomicroarcula nitratireducens sp. nov. (type strain F27T) and Halomicroarcula salinisoli sp. nov. (type strain F24AT). An in deep comparative genomic analysis of species of the genus Halomicroarcula, including their metabolism, their capability to biosynthesize secondary metabolites and their osmoregulatory adaptation mechanisms was carried out. Although they use a salt-in strategy, the identification of the complete pathways for the biosynthesis of the compatible solutes trehalose and glycine betaine, not identified before in any other haloarchaea, might suggest alternative osmoadaptation strategies for this group. This alternative osmoregulatory mechanism would allow this group of haloarchaea to be versatile and eco-physiologically successful in hypersaline environments and would justify the capability of the species of this genus to grow not only on environments with high salt concentrations [up to 30% (w/v) salts], but also under intermediate to low salinities.España Junta de Andalucía (grants US-1263771 [US/JUNTA/FEDER/UE], P20_01066 and BIO-213, which included FEDER funds)Spanish Ministry of Science and Innovation-State Research Agency /FEDER (projects CGL2017-83385-P and PID2020-118136GB-I00

Myopia and Other Visual Disorders in Children

Depto. de Optometría y VisiónFac. de Óptica y OptometríaTRUEpu

Natronomonas salsuginis sp. nov., a New Inhabitant of a Marine Solar Saltern

A halophilic archaeon, strain F20-122T, was isolated from a marine saltern of Isla Bacuta (Huelva, Spain). Cells were Gram-stain-negative, aerobic, and coccoid in morphology. It grew at 25-50 °C (optimum 37 °C), pH 6.5-9.0 (optimum pH 8.0), and 10-30% (w/v) total salts (optimum 25% salts). The phylogenetic analyses based on the 16S rRNA and rpoB' genes showed its affiliation with the genus Natronomonas and suggested its placement as a new species within this genus. The in silico DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) analyses of this strain against closely related species supported its placement in a new taxon. The DNA G + C content of this isolate was 63.0 mol%. The polar lipids of strain F20-122T were phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol (PG), and phosphatidylglycerol sulfate (PGS). Traces of biphosphatidylglycerol (BPG) and other minor phospholipids and unidentified glycolipids were also present. Based on the phylogenetic, genomic, phenotypic, and chemotaxonomic characterization, we propose strain F20-122T (= CCM 8891T = CECT 9564T = JCM 33320T) as the type strain of a new species within the genus Natronomonas, with the name Natronomonas salsuginis sp. nov. Rhodopsin-like sequence analysis of strain F20-122T revealed the presence of haloarchaeal proton pumps, suggesting a lightmediated ATP synthesis for this strain and a maximum wavelength absorption in the green spectrum.España MINECO through project CGL2017-83385-PEspaña, Junta de Andalucía (BIO-213, US-1263771

Prevalence of Dry Eyes Symptoms in Association with Contact Lenses and Refractive Status in Portugal

Background: Determine whether the presence of ocular symptoms in soft-contact-lens wearers changes depending on the refractive status. Methods: During the months of January to March 2022, the CLDEQ-8 questionnaire was administered to soft-contact-lens wearers. The statistical analysis was carried out using the SPSS 27.0 computer program (SPSS Inc., Chicago, IL, USA). Results: A total of 251 subjects participated in the study, with a higher percentage of myopes than hyperopes (82.1% versus 16.7%; p < 0.001). Out of all total participants, 21.5% suffered from dry-eye symptoms. It was noted that hyperopes presented a higher rate of dry-eye symptoms (p = 0.041). At the same time, the spherical equivalent was more positive in the participants with dry-eye symptoms (p = 0.014). Significant differences were found based on the symptoms present with contact lenses and the degree of myopia. The intensity of visual disturbances was higher in the participants with medium myopia (median [IQR]: 1/5 [2]) compared to those with low (median [IQR]: 0/5 [2]) and high myopia (median [IQR]: 0/5 [1]) (p = 0.009). Conclusions: Contact-lens wearers with hyperopia showed a higher rate of ocular dryness than those with myopia. In turn, wearing daily-replacement lenses could be one of the reasons for the lesser presence of ocular dryness compared to monthly-replacement lenses.Sección Deptal. de Óptica (Óptica)Fac. de Óptica y OptometríaTRUEpu

Equilibrium states for maps isotopic to Anosov

In this work we address the problem of existence and uniqueness (finiteness) of ergodic equilibrium states for partially hyperbolic diffeomorphisms isotopic to Anosov on T4, with two-dimensional center foliation. To do so we propose to study the disintegration of measures along one-dimensional subfoliations of the center bundle. Moreover, we obtain a more general result characterizing the disintegration of ergodic measures in our context.Fundação de Amparo à Pesquisa do Estado de São Paulo/[2018/18990-0]/FAPESP/BrasilCoordinación de la formación del personal de nivel superior/[2019/88882.329056-01]/CAPES/BrasilFundação de Amparo à Pesquisa do Estado de São Paulo/[17/06463-3]/FAPESP/BrasilFundação de Amparo à Pesquisa do Estado de São Paulo/[16/22475-9]/FAPESP/BrasilUniversidad de Costa Rica/[]/UCR/Costa RicaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de MatemáticaUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Matemáticas Puras y Aplicadas (CIMPA