Proteomics of Optic Ganglion from the Sepia esculenta and the Octopus vulgaris

金乌贼（Sepiaesculenta）和真蛸（Octopusvulgaris）属于软体动物门头足类（Cephalopodaclass）动物。头足类神经系统分化程度较高，易于解剖分离，适合于神经科学的研究。本论文采用双向凝胶电泳（2D-PAGE）技术分别分离金乌贼视神经节的全蛋白质，并比较了TCA/丙酮沉淀法、缓冲液法、柱层析法、裂解液法和裂解液-超速离心法分别分离和提取神经节蛋白质的优劣结果后，确立了以裂解液-超速离心法为提取神经节蛋白质的最佳方法。此外，还对载体两性电解质的pH范围、第一向等电聚焦（IEF）上样量、第二向聚丙稀酰胺凝胶电泳（SDS-PAGE）分离胶浓度等内容进行优化，建立了一...Sepia esculenta and Octopus vulgaris are both mollusk animals belonging to class Cephalopoda. The nervous system of Cephalopoda is relatively advanced, and the ganglion of Cephalopoda is easy to anatomise and separate, making it ideal for carrying out neuroscience researches. In this paper, the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was chosen to separate the total ...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：20042611

金乌贼视神经节蛋白质组分离方法的初步研究

选用TCA/丙酮沉淀法、缓冲液法、柱层析法、裂解液法和裂解液-超速离心法分别提取金乌贼视神经节蛋白质,其中蛋白质得率较高的方法有裂解液-超速离心法、裂解液法,其次为TCA/丙酮沉淀法.经双向凝胶电泳分离,并采用Melanie 4 Trial软件统计蛋白质斑点数目.发现pH 5~8范围的载体两性电解质适合于分离视神经节蛋白质组,其电泳图谱中多数蛋白质斑点清晰可见,显示出高分辩率、重复性好和易取样供质谱分析的优点,适合于开展视神经节蛋白质组学研究.裂解液-超速离心法是金乌贼视神经节最佳蛋白质提取方法

金乌贼脑和视神经节蛋白质组比较分析

采用双向凝胶电泳(2D-PAGE)技术优化分离金乌贼的脑及视神经节全蛋白质,并选用肽质量指纹谱(Peptide mass fingerprinting,PMF)技术和数据库检索方法对2D-PAGE图谱上的部分蛋白质斑点进行鉴定,初步构建了金乌贼视神经节(Optic ganglion ofSepia esculenta,SEOG)和脑神经节(Cerebral ganglion ofSepia es-culenta,SECG)部分分子解剖图谱.用Melanie 4 Trial软件分析脑神经节和视神经节蛋白质斑点总数量分别为682和594个,其中SECG蛋白质斑点数量明显多于SEOG.在脑神经节和视神经节中均发现了线粒体苹果酸脱氢酶前体(Mitochondrial malate dehydrogenase precursor,pre-MDH)及可溶性NSF连接蛋白(SNAP-typeproteins).此外,延长因子(Elongation factor G)、微管蛋白(Tubulin)和肌动蛋白(Actin)等蛋白质也具有高匹配率.已鉴定的蛋白质,多数归属于假定蛋白和结构蛋白类

深圳职业男性工作压力与性心理健康状况关系调查

性是人类的本能,是美好的。但在中国,性教育极度匮乏,性无知现象和性道德防线决堤现象并存。在性问题上,有人性冷淡,有人性泛滥,有人性焦虑,有人对性感到羞怯,等等。中国人的性心理健康是一个值得研究的课题。世界卫生组织对性心理健康所下的定义是:通过丰富和完善的人格、人际交往和爱情方式,达到性行为在肉体、感情、理智和社会诸方面的圆满和协调。认为性心理健康必须具备以下4个条件:一是个广东省计生委科研项目(编号:2004040)基金项目资

Electrodeposition and Lithium Storage Performance of Amorphous Fe-P Alloy Electrodes

应用电沉积技术制备了fE-P合金电极材料。采用X射线衍射(Xrd)和扫描电子显微镜(SEM)分析了该合金材料的相结构和表面形貌。Xrd分析结果表明电沉积的fE-P合金具有非晶态结构。电化学性能测试表明:平面结构的fE-P合金电极首次放电(脱锂)容量达542MAH.g-1,首次循环的库仑效率为60%;50周循环之后放电容量为366MAH.g-1。用非原位的Xrd和SEM对电极的充放电机理进行了初步研究,结果表明,首次充电(嵌锂)过程中形成lI3P相,电极表面生成纳米棒结构铁-磷合金,它能有效缓解锂嵌入/脱出时引起的合金结构变化,抑制合金材料的体积膨胀,从而提高该合金电极的充放电效率和循环性能。The planar structural Fe-P alloy deposits were prepared by electroplating.The structure and electrochem-ical performance of the electroplated Fe-P alloys have been investigated in detail.XRD results showed that Fe-P al-loy deposit exhibits an amorphous state.Electrochemical tests showed that the Fe-P alloy composite electrodes can deliver a discharge capacity of 542mAh.g-1 in the first cycle and the coulombic efficiency of 60%.At the 50th cycle the discharge capacity was 366mAh.g-1.The Fe-P electrodes with nanorods structure obtained during cycling was beneficial in reducing the irreversible capacity of Fe-P alloy electrode at initial charge-discharge,and in relaxing the volume expansion during cycling,which improved the cycleability of Fe-P alloy electrode.They are also beneficial to diffusion of Li inset/out of materials,and improve coulombic efficiency in charge-discharge cycle.国家自然科学基金(No.20773102、20833005);国家“973计划”(No.2009CB220102);国家基础科学人才培养基金(No.J0630429)项目资

Thrust and Practice of Super High-Yielding Rice Production in China

Corresponding author, zheng21006@ sohu.com[中文文摘]追求水稻高产再高产一直是中国人的夙愿。经过半个世纪长期不懈的努力 ,育种技术和栽培方法不断开拓创新 ,中国的水稻单产增长了 237倍 ,居世界高产大国 ,总产增长 3 13倍 ,占世界总产的35 % ,并取得了许多辉煌的科技成就。其中杂交水稻育种技术居世界领先水平 ,水稻最高单产屡屡突破世界纪录。本文列举了中国对水稻超高产执着追求的实例 ,回顾了中国水稻产量的提高历程 ,综述了水稻产量的理论极限及提高水稻产量的育种、栽培途径及高新技术的应用 ,展望了中国稻作未来的发展。[英文文摘]The long-cherished wish of Chinese is pursuing high and higher-yielding of rice. With long-term effort and practice in half a century, there is a continuous upgrading and innovat ion in rice breeding technologies and cult-ivation techniques. China had become the world”s biggest rice producer and made many great recog nized achievements in rice science and technolog y. The average yield of rice has risen from 1890kg•hm- 2 in 1949 to 6366 kg•hm- 2 in 1998, increasing by 2.37 times, and the total output has risen from 4864.4 million tons in1949 to 20073.7 million tons in 1997, increasing by 3.13 times, accounting for 35% of the world"s total output . Among them, hybrid rice breeding technology is still keeping ahead in the world and the highest yield perhectare frequently breaks the world record. This paper has introduced the cases of strongly pursuing super highyielding of rice and retrospected to the course of the development of rice production in China. The authers have analysed the upper limited protentials of rice product ion and also review ed the approachs for increasing rice yield potentials based on breeding and planting and adoption of biotechnologies. The future prospect s of rice development in China are put forw ard in this paper

Structure and Properties of Three-dimensional Reticular Sn-Co Alloy Electrodes as Anode Material for Lithium Batteries

应用电沉积技术制备了三维网状结构的Sn-Co合金负极材料,采用XRD、SEM和电化学方法考察了该负极材料的结构和性能.XRD分析表明,该三维网状结构的Sn-Co合金镀层为六方固溶体结构.其电化学性能测试表明:三维网状结构Sn-Co合金微晶电极的性能稳定,其首次放电容量高达493.4mAh·g-1,首次库仑效率达80.03%,而平面结构Sn-Co合金电极的首次库仑效率为63.47%.经50周充放电循环后,三维网状结构Sn-Co合金电极的放电容量为329.6mAh·g-1,放电容量保持率为66.8%;SEM分析表明:三维网状Sn-Co合金电极表面是由大小不一、高低不同的“岛”紧密排列在一起;“岛”和多孔结构的存在,缓冲了锂嵌入时体积的膨胀,部分抑制了材料结构的变化,减缓了电极容量的衰减,改善了电极的循环性能.The three-dimensional reticular Sn-Co alloy deposits were prepared by electroplating.The structure and electrochemical performance of the electroplated three-dimensional reticular Sn-Co alloys have been investigated in detail.Experimental results show that the Sn-Co alloy film is of hexagonal solid solution,with Sn as the solvent,Co as the solute.Electrochemical tests show that the three-dimensional reticular Sn-Co alloy coating electrodes can deliver a discharge capacity of 493.4 mAh·g-1 in the first cycle.At the 50th cycle the charge was 329.6 mAh·g-1.The three-dimensional reticular structure in Sn-Co alloy electrode was beneficial in reducing the irreversible capacity of Sn-Co alloy electrode at initial charge-discharge,and in relaxing the volume expansion during cycling,which improved the cyclability of Sn-Co alloy electrode.They are also beneficial to diffusion of Li into /out of macroporous materials,and improve coulomb efficiency in charge-discharge cycle.国家重点基础研究和发展规划(973项目,2002CB211804)资助项

Fabrication and Performance of Cu_6Sn_5 Alloy Anode Using Porous Cu as Current Collector

以氢气泡为动力学模板电沉积获得多孔铜,并通过热处理增强其结构稳定性.进一步将多孔铜作为基底通过电沉积制备Cu-Sn合金负极.Xrd结果给出其组成为Cu6Sn5合金,扫描电子显微镜(SEM)观察到Cu6Sn5合金电极为三维(3d)多孔结构.充放电结果指出,Cu6Sn5合金电极具有较好的充放电性能,其首次放电(嵌锂)和充电(脱锂)容量分别为735和571MAH·g-1,并且具有较好的容量保持率.运用电化学阻抗谱研究了Cu6Sn5合金电极在商业电解液中的界面特性.Porous Cu was fabricated by electrodeposition through a kinetic template of hydrogen bubbles.The product was subsequently annealed to increase its structural stability.The Cu-Sn alloy was then electrodeposited onto porous Cu which served as a current collector.X-ray diffraction (XRD) studies ascertained that the composition of the Cu-Sn alloy was Cu6Sn5 and scanning electron microscopy (SEM) investigations showed a three-dimensional (3D) porous structure of the electrode.The first charge/discharge capacities of the Cu6Sn5 alloy electrode were measured respectively at 735 and 571 mAh·g-1,and a good retention of the capacities has been determined.Interfacial properties of the Cu6Sn5 alloy electrode in a commercial electrolyte were also studied by electrochemical impedance spectroscopy (EIS).国家重点基础研究发展规划(973)项目(2009CB220102)资

Electrochemical Impedance Spectroscopy Study on Phase Transformation of Cu_6Sn_5 Alloy Anode

以粗糙铜箔为基底,采用一步电沉积法获得Cu-Sn合金,X射线衍射(Xrd)测试结果显示其主要为Cu6Sn5合金相.扫描电子显微镜(SEM)测试结果表明该合金表面由大量“小岛“组成,且每个“小岛“上存在大量纳米合金粒子.充放电测试结果表明,以该合金为锂离子电池负极,其初始放电(嵌锂)和充电(脱锂)容量分别为461和405MAH·g-1.电化学阻抗谱测试结果显示,Cu6Sn5合金电极在阴极极化过程中分别出现了代表固体电解质界面膜(SEI膜)阻抗、电荷传递阻抗和相变阻抗的圆弧,并详细分析了它们的变化规律.The Cu-Sn alloy electrode was prepared by a one-step electrodepositing method using rough Cu foil as the substrate,and was determined as the intermetallic composite of Cu6Sn5 using an X-ray diffraction(XRD) method.The electrode surface morphology was analyzed by scanning electron microscopy(SEM) which displayed "small islands" structure with many nano-particles on it.The first discharge and charge capacities were determined as 461 and 405 mAh·g-1,respectively.Electrochemical impedance spectra(EIS) indicated that there appeared three arcs in the Nyquist plots respectively representing the impedance of solid electrolyte interphase film,charge transfer and phase transformation in the first lithiation,and their evolutive principles were also investigated.国家重点基础研究和发展规划(973)(No.2009CB220102);国家自然科学基金(No.20773102)资助项

Proteomic Analysis of Rice Cultivar Jiafuzhan in the Responses to Xanthomonas campestris pv.oryzicola Infection

作者简介: 陈芳育(1978-) , 男, 讲师。E-mail : cfy307@ sohu. com * 通讯作者(Corresponding author) : 陈亮( 1963-) , 男, 教授, 博士生导师, 研究方向: 细胞与分子生物学。E-mail: chenlg@ xmu. edu. cn[中文文摘]运用双向电泳分析高抗水稻品种“佳辐占”受强毒力细菌性条斑病病原菌侵染2d后的叶片蛋白质组变化,共发现38个蛋白质发生差异表达,其中32个上调,5个下调,1个新增。用MALDI-TOF-MS分析和数据库检索鉴定出其中的33个差异表达蛋白质,并将它们分为4个功能类群,即信号转导相关蛋白、防卫相关蛋白、代谢相关蛋白和蛋白质稳定相关蛋白。这些蛋白分别参与了信号识别、信号传递、抗氧化、糖代谢、细胞壁加固、植保素合成等抗病生理反应。研究表明,水稻对细菌性条斑病病原菌的侵染存在着一个复杂的抗病信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质(酶)反映出来,其中差异表达的8个R蛋白和3个PR蛋白可能与水稻对细菌性条斑病的抗病性密切相关。本研究为进一步揭示水稻对细菌性条斑病的抗性机理及相关抗病基因的功能克隆提供了依据。[英文文摘]Rice bacterial leaf streak( BLS) caused by the pathogen Xanthomonas oryzae pv. oryzicola ( Xooc) is one of the major rice diseases in South China. Here we focus on proteomics as a tool for the discovery of differentially expressed proteins closely related to the disease resistance. The leaves of rice cultivar Jiafuzhan (Oryzae sativa L. ) highly resistant to the disease, were infected by"89773-1- 1" strain of the Xooc with strong pathogenicity. Total proteins were extracted from the leaves sampled at two days after inoculation, and separated by two- dimensional electrophoresis. It was found that there were thirty- eight proteins expressed differentially, of which thirty-two were up-regulated, five down-regulated and one was "new". Of the thirty- eight responsive proteins, thirty-three were identified by MALD-I TOF-MS and database searching.Based on the predicted function, we grouped them into four clusters: signal transduction, defensive responses, substance metabolism and protein stabilization, which were involved in many resistant physiological react ions, including signal recognition and transduction, antioxidant react ion, carbonhydrate metabolism, cel-l wall reinforcement and phytoalexin biosythesis. In turn a complex signal transduct ion and metabolic regulative network in the resistant responses to the infection of Xooc was outlined in this work, and the molecular mechanism was revealed by differentially expressed protein/enzyme patterns during Xooc infection. In this study, eight R proteins and three pathogenesis- related(PR) proteins which might relate closely to the disease-resistance were found. This result provides us the basic information to further reveal the resistant mechanism and conduct functional cloning of the resistan-t related genes in rice to BLS.生物农药与化学生物学教育部重点实验室( 福建农林大学) 开放课题基金项目( KF0411