目的探讨冠心病(CHD)患者经皮冠状动脉腔内成形术(PTCA)、冠状动脉造影(CA)手术前及术后中性粒细胞(PMN)和单核细胞CD11b表达变化及临床意义。方法选择66例CHD患者,其中41例行PTCA手术、25例行CA手术,23例健康体检者为研究对象。采用流式细胞仪检测健康体检者及CHD患者PTCA和CA手术前3d、手术当天、术后30min、术后1d、术后3d外周血PMN和单核细胞表面CD11b的表达。结果CHD患者外周血PMN和单核细胞CD11b平均荧光强度(MFI)较正常对照组明显升高,差异具有显著性(P0.05)。结论PMN和单核细胞表面CD11b含量评价选择平均荧光强度比选择百分率更能反映CD11b的变化。CHD患者PTCA术后确有炎症反应发生,CD11b表达可作为PTCA后炎症反应发生有临床应用价值的指标。Objective: To investigate changes in the expression of CD11b on neutrophils and monocytes before and after PTCA and CA in patients with coronary heart disease (CHD).Methods:Forty-one patient sunderwent percutaneous coronary angioplasty (PTCA). Twenty-five patients underwent coronary angiographic (CA). Twenty-three people were healthy as control. Peripheral venous blood samples were obtained at intraday, 30min, 24h and 72h before and after PTCA and CA. Flow-cytometric methods were used to measure CD11b on neutrophils and monocytes. Rsults: The levels of CD11b on neutrophils and monocytes were significantly higher in UA patients with CHD compared to controls (P0.05). Conclusion: The mean intensity of fluorescence is better than percentage to evaluate the content of CD11b on neutrophils and monocyte. PTCA triggers leukocytes activation in circulation. CD11b can reflect the acute inflammatory activity aridses which arises after PTCA
