Regulation and biochemistry of mouse molybdo-flavoenzymes: The DBA/2 mouse is selectively deficient in the expression of aldehyde oxidase homologues 1 and 2 and represents a unique source for the purification and characterization of aldehyde oxidase

Mouse molybdo-flavoenzymes consist of xanthine oxidoreductase, aldehyde oxidase (AOX1), and two recently identified proteins, AOH1 and AOH2 (aldehyde oxidase homologues 1 and 2). Here we demonstrate that CD-1, C57BL/6, 129/Sv, and other mouse strains synthesize high levels of AOH1 in the liver and AOH2 in the skin. By contrast, the DBA/2 and CBA strains are unique, having a selective deficit in the expression of the AOH1 and AOH2 genes. DBA/2 animals synthesize trace amounts of a catalytically active AOH1 protein. However, relative to CD-1 animals, an over 2 log reduction in the steady-state levels of liver AOH1 mRNA, protein, and enzymatic activity is observed in basal conditions and following administration of testosterone. The DBA/2 mouse represents a unique opportunity to purify AOX1 and compare its enzymatic characteristics to those of the AOH1 protein. The spectroscopy and biochemistry of AOX1 are very similar to those of AOH1 except for a differential sensitivity to the non-competitive inhibitory effect of norharmane. AOX1 and AOH1 oxidize an overlapping set of aldehydes and heterocycles. For most compounds, the substrate efficiency (V(max)/K(m)) of AOX1 is superior to that of AOH1. Alkylic alcohols and acetaldehyde, the toxic metabolite of ethanol, are poor substrates of both enzymes. Consistent with this, the levels of acetaldehyde in the livers of ethanol administered CD-1 and DBA/2 mice are similar, indicating that neither enzyme is involved in the in vivo biotransformation of acetaldehyde

Algumas observações sobre o futuro do modo conjuntivo

The Portuguese uses future subjunctive (futuro do conjuntivo) in subordinate clauses to express a condition that must be fulfilled in the future, or is assumed to be fulfilled, before an event can happen. As the other Romance languages don't know or don't regularly use this form of subjunctive, the author aims to find its equivalents in Spanish and French in various syntactic contexts. The main purpose is to specify the position of the future subjunctive within the Portuguese verbal system. If we imagine that the future subjunctive dissapears from the Portuguese verbal system and gets replaced by the most convenient tense for each context, no significant semanthic changes could be noticed in the vaste majority of cases. It could be easily substituted by the present subjunctive in conditional, concessive and partially also adverbial and relative clauses. However, the future subjunctive has different - more factual and definit - meaning than the present subjunctive in some specific adverbial and relative clauses. It means that these clauses admit both sunjunctive tenses in Portuguese but with slightly different semanthic meanings. This difference doesn't have equivalent in the other Romance languagues. In general, the use of subjuntive forms in Portuguese can be seen as an important formal indicator of the semanthic value of certain syntactical constructions: the verb in the indicative mood means the sentence has a repetitive value (Modus Ponens) whereas the verb in the subjunctive mood expresses an eventuality in the future. In this perspective the Portuguese also has an exceptional position within the Romance language area. Spanish and French don't seem to use the formal indicator or at least in the contexts we have seen

Concordância negativa em português e a sua evolução

The Sentential negation, and the negative concord in particular, is a complex phenomenon which is far from being diachronically invariable. We could observe on the example of Portuguese that the main reason for the diachronic variation in the field of negation is the changing semantical status of the sentential negative marker não, according to the theory developed by Zeijlstra and Penka. The non-strict negative concord language as it is now has developed from Archaic Portuguese which could be considered as a strict NC language. The negative marker was originally required to accompany the semantically negated verb at any time, even in the co-occurrence with pre-verbal n-words. The negative marker was semantically non-negative which means that it had to participate, together with the remaining negative elements in the sentence, in a feature checking relation with an abstract negative operator, free of phonological content. Later on, in the Contemporary Standard Portuguese, the negative marker adopted the function of semantically negative operator against which other negative elements in the sentence need to be checked. Where the negative marker couldn´t license the n-words due to syntactical constraints it was not desirable, for the economy reasons, to appear anymore and was simply replaced by the abstract negative operator. The admitting of new structures of sentential negation in the Contemporary Colloquial Brazilian Portuguese is a proof that the system of sentential negation in Portuguese tends to evolve further in the common direction described by Jespersen. The Contemporary Colloquial Brazilian Portuguese is without doubts still a non-strict NC language which makes use of the negation marker não in a different way than it is common in the Standard Portuguese: it places it in the sentence final position to give a negative interpretation to the content of the sentence and to emphasize it at the same time

CESNET/perun: Release v3.11.3

Identity and Access Management Syste

Diethoxycarbonyl-BODIPYs as heavy-atom-free photosensitizers for holographic recording in cellulose acetate photopolymer

A series of new heavy-atom-free photosensitizers based on 2,6-diethoxycarbonyl-BODIPY scaffold was designed and applied for holographic recording in a photopolymerizable material. Photoinduced electron transfer between the BODIPY and meso-aryl subunits, followed by the formation of BODIPY triplet excited states via spin-orbit charge transfer intersystem crossing (SOCT-ISC) was studied by steady-state and ultrafast pump-probe transient optical spectroscopy. Highly efficient photosensitization was observed for dyads bearing pyrene and anthracene substituents, which exhibited singlet oxygen generation quantum yields () of up to 94%. Charge transfer and SOCT-ISC were observed in non-polar solvent (toluene) due to the increased electron accepting ability of the diethoxycarbonyl-BODIPY. In combination with N-phenylglycin (NPG) as a co-initiator, new BODIPYs initiate a free-radical polymerization of acrylamide monomers under 532 nm irradiation that was used for creation of volume phase transmission gratings in a photopolymerizable material based on cellulose acetate and polyethylene glycol (CA-PEG). As a result of holographic recording, diffractive structures with diffraction efficiency of up to 56% were obtained for CA-PEG layers sensitized with BODIPY-pyrene dyad as compared to a reference heavy-atom-containing diiodo-BODIPY dye (27%). The developed materials showed refractive index modulation of up to 2.3 × 10-3, which demonstrate the potential of diethoxycarbonyl-BODIPYs photosensitizers for holographic recording applications

Diethoxycarbonyl-BODIPYs as heavy-atom-free photosensitizers for holographic recording in cellulose acetate photopolymer

A series of new heavy-atom-free photosensitizers using 2,6-diethoxycarbonyl-BODIPY was designed and synthesized. Photoinduced electron transfer between the BODIPY and meso-aryl subunits followed by the formation of BODIPY triplet excited states via spin-orbit charge transfer intersystem crossing (SOCT-ISC) was studied by steady-state and ultrafast pump-probe transient optical spectroscopy. Highly efficient photosensitization was observed for dyads bearing pyrene and anthracene substituents, which exhibited singlet oxygen generation with quantum yield of up to 94%. Notably, charge transfer and ISC were observed in non-polar solvent (toluene) due to the increased electron accepting ability of the diethoxycarbonyl-BODIPY as compared to alkyl-substituted BODIPY scaffold. Studied dyads were used as photosensitizers for creation of volume phase transmission gratings in a photopolymerizable material based on cellulose acetate - polyethylene glycol (CA-PEG) matrix. In combination with N-phenylglycin (NPG) as a co-initiator, new BODIPYs showed the ability to initiate a free-radical polymerization of acrylamide monomers under 532 nm irradiation. This allowed for holographic recording of diffractive structures with diffraction efficiency of up to 56% obtained for CA-PEG layers with BODIPY-pyrene dyad as compared to a reference heavy-atom-containing diiodo-BODIPY dye (27%). The materials sensitized with diethoxycarbonyl-BODIPYs showed refractive index modulation of up to 2.3 × 10-3 during holographic recording at the spatial frequency of 800 lines/mm which demonstrate the potential of SOCT-ISC photosensitizers in photopolymerization and holographic recording applications

CESNET/perun: v21.0.2

<a href="https://github.com/CESNET/perun/compare/v21.0.1...v21.0.2">21.0.2</a> (2023-01-16) Bug Fixes <ul> <li>remove apostrophe from ldif (<a href="https://github.com/CESNET/perun/commit/509ecd49101c758b05987d564665dd1eb3b629a2">509ecd4</a>)</li> </ul&gt

CESNET/perun: v19.0.0

<a href="https://github.com/CESNET/perun/compare/v18.0.1...v19.0.0">19.0.0</a> (2022-11-04) Bug Fixes <ul> <li><strong>cli:</strong> default OIDC config message fix (<a href="https://github.com/CESNET/perun/commit/21ac217c97a74114ccf6d8a147e979eb70a0da31">21ac217</a>)</li> <li><strong>cli:</strong> fixed printing help for reloadAppsConfig tool (<a href="https://github.com/CESNET/perun/commit/6e18883737cefce9076cdd5dd8dea2f422e54632">6e18883</a>)</li> <li><strong>core:</strong> fix createGroupUnion sql exception (<a href="https://github.com/CESNET/perun/commit/bdf41f58faba65ce466fdfa4c161b7e4ec905f52">bdf41f5</a>)</li> <li><strong>core:</strong> fix getMessagesByIdAndCount (<a href="https://github.com/CESNET/perun/commit/aa281f846a813475e6e868c2c8a9ab33f0f902aa">aa281f8</a>)</li> <li><strong>core:</strong> fixed ssh keys validation for keys with prefix (<a href="https://github.com/CESNET/perun/commit/64b5e7192886c8d05ce3ad614fc785d639a8f3da">64b5e71</a>)</li> <li><strong>core:</strong> support mu password manager on test instance (<a href="https://github.com/CESNET/perun/commit/05311cbc70f189ac3bd66d266f4b34a9248f2e77">05311cb</a>)</li> <li><strong>registrar:</strong> check form items on update (<a href="https://github.com/CESNET/perun/commit/2bab3ee582fcdc0cf8ec68318c246c7a1c36b513">2bab3ee</a>)</li> <li><strong>registrar:</strong> fix duplicate notifications (<a href="https://github.com/CESNET/perun/commit/2023cb60c57d3d4bbc83dfd61e03bca468edaf3c">2023cb6</a>)</li> </ul> Features <ul> <li>Add method to get OIDC config for Perun instance + CLi script to call this method (<a href="https://github.com/CESNET/perun/commit/3b54deccfe971b91c44aaac4b552c29397137511">3b54dec</a>)</li> <li>added support for securityText attribute (<a href="https://github.com/CESNET/perun/commit/eb7ff3ab581fa48d93723460da57b85a1dd45df8">eb7ff3a</a>)</li> <li><strong>cabinet:</strong> rights changed for getAuthorsByPublicationId method (<a href="https://github.com/CESNET/perun/commit/7da850b860ccb9fde48c42a3ea1ff5e5db0391a4">7da850b</a>)</li> <li><strong>cli:</strong> revokeOidc fallback (<a href="https://github.com/CESNET/perun/commit/06d05617a3406c941dda59efe89176863257cf5f">06d0561</a>)</li> <li><strong>core:</strong> add ConsentStatus to UsersPageQuery (<a href="https://github.com/CESNET/perun/commit/2c861a56fc99520f679ce2fdadc4289644dca9c4">2c861a5</a>)</li> <li><strong>core:</strong> allow self to call get all entities (<a href="https://github.com/CESNET/perun/commit/b0d38a71e4663667d85c6203d7910e187efd01e7">b0d38a7</a>)</li> <li><strong>core:</strong> Default minimum password length set to 8 characters (<a href="https://github.com/CESNET/perun/commit/c6835da36ec982c1870ec59cebacaf9ed7d5aadb">c6835da</a>)</li> <li><strong>core:</strong> exact match user attribute list/map values (<a href="https://github.com/CESNET/perun/commit/d710e8b93cde778281e2c6263bd5cb74202ab29b">d710e8b</a>)</li> <li><strong>core:</strong> getAllResources method (<a href="https://github.com/CESNET/perun/commit/6888a17062b4885044b4ac128b32da36c94f72ec">6888a17</a>)</li> <li><strong>core:</strong> Role display name (<a href="https://github.com/CESNET/perun/commit/4cf8b84683ac8e6400754a7fe075c1ea6c41d8cb">4cf8b84</a>)</li> <li><strong>core:</strong> status indirect urn (<a href="https://github.com/CESNET/perun/commit/806073bbfc3bdd93bd6e947db794ba8e78df2ade">806073b</a>)</li> <li><strong>core:</strong> support branding between new and old GUI (<a href="https://github.com/CESNET/perun/commit/f947e7a0a1b4c2c4954c289720fbb0a551a5613b">f947e7a</a>)</li> <li>make find_group_inconsistencies script use user IDS to work better with hierarchical Vos (<a href="https://github.com/CESNET/perun/commit/f282594c94f32f63f9bc09c751df6a76a41a8cd1">f282594</a>)</li> <li><strong>mvn:</strong> upgrade to Spring Boot 2.7.5 (<a href="https://github.com/CESNET/perun/commit/457024f4a7cdfb0fac0d7d69cad1435dfb2c95e7">457024f</a>)</li> <li><strong>openapi:</strong> (Un)set role for user or group (<a href="https://github.com/CESNET/perun/commit/f4326ffbcc6c8c2edb9bf70d31d07c096d2b7707">f4326ff</a>)</li> <li><strong>registrar:</strong> added ApplicationNotNewException (<a href="https://github.com/CESNET/perun/commit/f96c1c991289f7f2bedaa48e1574af34577654c5">f96c1c9</a>)</li> </ul> BREAKING CHANGES <ul> <li><strong>core:</strong> New brand properties in perun-apps-config.yml, deprecated newAdminGuiAlert in perun-web-gui.properties moved to perun-apps-config and can be branded</li> <li><strong>registrar:</strong> update DB</li> </ul&gt

CESNET/perun: v17.0.0

<a href="https://github.com/CESNET/perun/compare/v16.0.1...v17.0.0">17.0.0</a> (2022-08-09) Bug Fixes <ul> <li><strong>core:</strong> add privilege for members to get facilities and resources (<a href="https://github.com/CESNET/perun/commit/6cffd0d69e33e357c45b804c0b983527b5ecd1fd">6cffd0d</a>)</li> <li><strong>core:</strong> fix rights for method findRichUsersWithAttributes (<a href="https://github.com/CESNET/perun/commit/419751ce33551dc7c86cc05b1232e12f3459da33">419751c</a>)</li> <li><strong>core:</strong> fixed error handling from calls to userInfoEndpoint (<a href="https://github.com/CESNET/perun/commit/789f02442effc43bf22bc36c9b537a6b28dbafba">789f024</a>)</li> <li><strong>core:</strong> remove old attribute authz if deleting attribute definition (<a href="https://github.com/CESNET/perun/commit/37c2fad0dc8acbeeff996f2cc7f619123bcd9392">37c2fad</a>)</li> <li><strong>dispatcher:</strong> do not put tasks without destination to waiting queue (<a href="https://github.com/CESNET/perun/commit/ab4fce5406d41ada789220aa18ae6afd82c665c5">ab4fce5</a>)</li> <li><strong>openapi:</strong> send password in request body (<a href="https://github.com/CESNET/perun/commit/bf3a94a23ac5c177e1f2ad15b41c860e2c598e81">bf3a94a</a>)</li> </ul> Features <ul> <li><strong>cli:</strong> default OIDC configuration (<a href="https://github.com/CESNET/perun/commit/7e606e677bf72b550c83735afe7a33a72809dd07">7e606e6</a>)</li> <li><strong>core:</strong> add sponsorship and membership to getUserRoles result (<a href="https://github.com/CESNET/perun/commit/1d021c8d997c46beaac4c1ef99bcee9903d25d8d">1d021c8</a>)</li> <li><strong>core:</strong> added consolidate method and call to userInfoEndpoint when creating Perun Principal (<a href="https://github.com/CESNET/perun/commit/024a5732bd23b55ab65e751768d892cf7ebfb9ab">024a573</a>)</li> <li><strong>core:</strong> changed lifescienceID module (<a href="https://github.com/CESNET/perun/commit/8e88cd3b8e9cc1ab567ccd82970c4c477a5a10f2">8e88cd3</a>)</li> <li><strong>core:</strong> extended LDAP schema with serviceName for facility (<a href="https://github.com/CESNET/perun/commit/9bdfc94cf3153146405c3f0fd36dd302ace47047">9bdfc94</a>)</li> <li><strong>core:</strong> fill bbmri/ls/elixir logins with each others values (<a href="https://github.com/CESNET/perun/commit/6ec0485ac6972f4b5dabcc7e9889c971f6ce5016">6ec0485</a>)</li> <li><strong>core:</strong> method for obtaining mailing list resources for given member (<a href="https://github.com/CESNET/perun/commit/f25fb884f9868e884806e2be4ac90a30323471d2">f25fb88</a>)</li> <li><strong>core:</strong> observer roles not assignable to attributes (<a href="https://github.com/CESNET/perun/commit/5e107f00751d794b7b2db96993dfacf9d0f3d1f1">5e107f0</a>)</li> <li><strong>core:</strong> push new attributes to LDAP (<a href="https://github.com/CESNET/perun/commit/b812236a939bd82819d2f58459d8235513ab9545">b812236</a>)</li> <li>mfaEnforceSettings and rpCategory LDAP attributes (<a href="https://github.com/CESNET/perun/commit/faf90b022788f6f92faafa4abfa0557773cc35c3">faf90b0</a>)</li> <li><strong>mvn:</strong> upgrade to Spring Boot 2.7.2 / Spring 5.3.22 (<a href="https://github.com/CESNET/perun/commit/ebf4967dc43be4c19ed439c11f5f1c84777fa1ba">ebf4967</a>)</li> <li>new method getMessagesByIdAndCount (<a href="https://github.com/CESNET/perun/commit/d06129ba9e3950ca93c37a394553332df4c7ef3c">d06129b</a>)</li> <li><strong>openapi:</strong> added method getGroupsWhereMemberIsActive (<a href="https://github.com/CESNET/perun/commit/7a6ba550af8c51ff1ed92bc90ce0061ed18eecce">7a6ba55</a>)</li> <li><strong>openapi:</strong> openapi updated to contain new method (<a href="https://github.com/CESNET/perun/commit/a494e0e97b1e1aaa1fe1f773088d44dac37ddf29">a494e0e</a>)</li> </ul> BREAKING CHANGES <ul> <li><strong>core:</strong> LDAP schema update</li> </ul&gt

CESNET/perun-services: Release 3.28.0

<ul> <li>CN and DN attribute values are now escaped in ad_ceitec.</li> <li>mailaliases and ad_user_mu_ucn now use the same attribute for allowing expired members.</li> <li>Extended grace period for students in VUP scripts.</li> <li>Multiple gen scripts converted to use getHashedData.</li> </ul&gt