758 research outputs found
Le jugement de valeur en littĂ©rature : le comparatisme au service de lâĂ©valuation artistique
info:eu-repo/semantics/publishe
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing.
Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of cellular heterogeneity in biological systems. However, single-cell sequencing of large populations has been hampered by limitations in processing genomes for sequencing. In this paper, we describe a method for single-cell genome sequencing (SiC-seq) which uses droplet microfluidics to isolate, amplify, and barcode the genomes of single cells. Cell encapsulation in microgels allows the compartmentalized purification and tagmentation of DNA, while a microfluidic merger efficiently pairs each genome with a unique single-cell oligonucleotide barcode, allowing >50,000 single cells to be sequenced per run. The sequencing data is demultiplexed by barcode, generating groups of reads originating from single cells. As a high-throughput and low-bias method of single-cell sequencing, SiC-seq will enable a broader range of genomic studies targeted at diverse cell populations
Confronting Forensics: Bullcoming v. New Mexico and the Sixth Amendment
The U.S. Supreme Courtâs 2004 Crawford v. Washington decision drastically altered the long-standing Confrontation Clause jurisprudence, refocusing the constitutional inquiry on the testimonial nature of a witnessâs statement but leaving for another day any effort to spell out a comprehensive definition of the term âtestimonial.â Thus began the current line of Confrontation Clause cases, each of which sought to clarify the Sixth Amendmentâs confrontation protections but arguably clouded any clarity that the case before it brought. In 2009, the Court decided Melendez-Diaz v. Massachusetts, in which it held that a forensic laboratory report prepared for a criminal trial is âtestimonialâ and that it therefore triggers the Confrontation Clause. Most recently, in 2010, the Court decided Bullcoming v. New Mexico and answered the question that Melendez-Diaz left open: if a forensic laboratory report triggers the Confrontation Clause, who must provide the live, in-court testimony? In a controversial 5â4 decision, the Court held that the analyst who actually conducted the forensic test and certified the report must take the stand, and that a so-called surrogate witness does not satisfy the constitutional requirement. This Comment suggests that the Court accurately assessed the fallibility of forensic science and correctly decided Bullcoming in a manner that was consistent with the Confrontation Clauseâs purposes
Classifying and mapping diversity in a species-poor system: the mangrove meta-community of Laguna Chacahua National Park, Oaxaca, Mexico
ABSTRACT
Classifying and Mapping Diversity in a Species-Poor System: the mangrove meta-community of Laguna Chacahua National Park, Oaxaca, Mexico
by
Elizabeth Kay Weisgerber
Both field transects and imagery grid plots were analyzed with the goal of creating a community classification map for the mangrove forest of Parque Nacional Lagunas de Chacahua. In total, data was collected in 49 sites throughout the park, recording measures such as DBH, basal area, estimated dominance, frequency, cover and relative dominance. Field locations were marked and georeferenced with a GPS and grid plots overlaid on satellite imagery of the park were generated via a random number table. Species\u27 attributes delineating the identifiable features for each dominant species within Chacahua were noted and used to recognize patterns in species assemblages.
Fourteen distinct âsub-communitiesâ within the mangrove meta-community were recognized using this data and verified with 165 field photos. Relative dominance values were compared between field and grid data. These data revealed a similar pattern with Rhizophora mangle being most dominant under each method. Avicennia germinans was second in relative dominance, Laguncularia racemosa a close third while Conocarpus erectus was rarely found, most likely due to over-harvesting.
Rapid degradation of mangrove forests is occurring on a global scale. Understanding the complex dynamics that occur within the mangrove meta-community is essential to its conservation. Vegetation maps are essential tool in monitoring changes throughout the mangrove but are rarely of sufficient detail for everyday use. Generating highly detailed vegetation maps in a cost-effective and timely manner is an important step for their conservation, particularly in developing countries. This study demonstrates methods and techniques for producing a vegetation map that portrays the level of complexity that exists within the species-poor mangrove environment. This map will be donated to the management team of Parque Nacional Lagunas de Chacahua to better aid in management
Die 14. Deutsche ArchÀologische Oman-Expedition 1995
Die GelĂ€ndekampagne hatte als Hauptziel die Kartierung und Untersuchung von einem GrĂ€berfeld, worin 59 TĂŒrme des frĂŒhen bis mittleren 3. vorchristlichen Jahrtausends erhalten sind. Derartige GrĂ€ber wurden noch nicht untersucht und schienen einen Ăbergang zu bilden zwischen Hafit und Umm an-Nar-zeitlichen Grabtypen. Da KulturdenkmĂ€ler wie prĂ€historische GrĂ€berfelder in der Praxis schwer zu schĂŒtzen sind, war diese Aufgabe wichtig. Eine Reihe interessanter Entdeckungen kamen hinzu: In al-BatÄ«n ein bisher unbekannter Schlackentyp wurde untersucht, die in die Hafit-Zeit zu gehören scheint. Im westlichen Hinterland des Sultanats bei Amlah in Qorin es-SahhÄimah kamen Grabreste vor, die unbekannt waren. Der Erhaltungszustand diverser DenkmĂ€ler wurde beobachtet
Samad ash-Shan, Preliminary Report 1988
Das sĂŒdöstliche Arabien â ein untererforschtes Teil des Vorderen Orients â wird meist als Teil der parthischen und sasanidischen Hoheiten betrachtet. Schwerpunkt unseres DFG-getragenen Projekts war es, von dieser vordergrĂŒndigen Ansicht abzuweichen und zu versuchen interne Quellen zusammen zu tragen. Es handelt sich um den ersten Vorbericht des Projekts (1987-1991)
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