Deciphering Charging Status, Absolute Quantum Efficiency, and Absorption Cross Section of MultiCarrier States in Single Colloidal Quantum Dot

Upon photo- or electrical-excitation, colloidal quantum dots (QDs) are often found in multi-carrier states due to multi-photon absorption and photo-charging of the QDs. While many of these multi-carrier states are observed in single-dot spectroscopy, their properties are not well studied due to random charging/discharging, emission intensity intermittency, and uncontrolled surface defects of single QD. Here we report in-situ deciphering the charging status, and precisely assessing the absorption cross section, and determining the absolute emission quantum yield of mono-exciton and biexciton states for neutral, positively-charged, and negatively-charged single core/shell CdSe/CdS QD. We uncover very different photon statistics of the three charge states in single QD and unambiguously identify their charge sign together with the information of their photoluminescence decay dynamics. We then show their distinct photoluminescence saturation behaviors and evaluated the absolute values of absorption cross sections and quantum efficiencies of monoexcitons and biexcitons. We demonstrate that addition of an extra hole or electron in a QD changes not only its emission properties but also varies its absorption cross section

Spectrum-Guided Adversarial Disparity Learning

It has been a significant challenge to portray intraclass disparity precisely in the area of activity recognition, as it requires a robust representation of the correlation between subject-specific variation for each activity class. In this work, we propose a novel end-to-end knowledge directed adversarial learning framework, which portrays the class-conditioned intraclass disparity using two competitive encoding distributions and learns the purified latent codes by denoising learned disparity. Furthermore, the domain knowledge is incorporated in an unsupervised manner to guide the optimization and further boosts the performance. The experiments on four HAR benchmark datasets demonstrate the robustness and generalization of our proposed methods over a set of state-of-the-art. We further prove the effectiveness of automatic domain knowledge incorporation in performance enhancement

Reordering Webpage Objects for Optimizing Quality-of-Experience

The quality of experience (QoE) perceived by users is a critical performance measure for Web browsing. “Above-The-Fold” (ATF) time has been recently recognized and widely used as a direct measure of user-end QoE by a number of studies. To reduce the ATF time, the existing works mainly focus on reducing the delay of networking. However, we observe that the webpage structures and content orders can also significantly affect theWeb QoE. In this paper, we propose a novel optimization framework that reorders the webpage objects to minimize the user-end ATF time. Our core idea is to first identify the webpage objects that consume the ATF time but have no impact on the page experience and then change the positions of these objects to achieve the minimum ATF time. We implement this framework and evaluate its performance with popular websites. The results show that the ATF time is greatly reduced compared with the existing works, especially for complex webpages

Use of the 2A Peptide for Generation of Multi-Transgenic Pigs through a Single Round of Nuclear Transfer

Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation. Most multi-transgenic pigs have been produced by complex and time-consuming breeding programs using multiple single-transgenic pigs. This study explored the feasibility of producing multi-transgenic pigs using the viral 2A peptide in the light of previous research indicating that it can be utilized for multi-gene transfer in gene therapy and somatic cell reprogramming. A 2A peptide-based double-promoter expression vector that mediated the expression of four fluorescent proteins was constructed and transfected into primary porcine fetal fibroblasts. Cell colonies (54.3%) formed under G418 selection co-expressed the four fluorescent proteins at uniformly high levels. The reconstructed embryos, which were obtained by somatic cell nuclear transfer and confirmed to express the four fluorescent proteins evenly, were transplanted into seven recipient gilts. Eleven piglets were delivered by two gilts, and seven of them co-expressed the four fluorescent proteins at equivalently high levels in various tissues. The fluorescence intensities were directly observed at the nose, hoof and tongue using goggles. The results suggest that the strategy of combining the 2A peptide and double promoters efficiently mediates the co-expression of the four fluorescent proteins in pigs and is hence a promising methodology to generate multi-transgenic pigs by a single nuclear transfer

Gut Microbial Compositions in Four Age Groups of Tibetan Minipigs

In this study, the gut microbiota was characterized in four age strata of Tibetan minipigs. Results indicated that the fecal bacteria of 7-, 28-, 56-, and 180-day-old minipigs did not significantly differ in terms of phylogenetic diversity (i.e., PD whole tree) or the Shannon index (both, p > 0.05). Findings of a principal coordinate analysis demonstrated that fecal bacteria of 180-day-old minipigs were discernable from those of the other three age groups. From ages seven to 56 days, the abundance of Bacteroidetes or Firmicutes appeared to vary. Regarding genera, the populations of Bacteroides and Akkermansia decreased with increasing age

Muscular kevlar aerogel tapes attractive to thermal insulation fabrics

Kevlar aerogel is a kind of easy-casting nano-porous material with the characteristics of low density, high porosity, low thermal conductivity, high specific surface area, etc. It can serve as functional fibers and has a bright future in the field of thermal insulation after being processed into fabrics. To improve the tensile power of aerogel fibers to bear more loads while maintaining their flexibility for further braiding, a series of Kevlar aerogel tapes were fabricated in this study by using flattened needles. It is observed that the resultant aerogel tapes show a spindle-like cross section, and have greatly improved load-bearing capability with muscular tensile strength up to 2.07 MPa. The use of a larger needle is conductive to preparing aerogel tapes that are more attractive in load-bearing, and increasing Kevlar concentration tends to result in more muscular tapes with improved breaking stress but reduced elasticity. Besides, these tapes also inherit the excellent thermal stability and char-forming ability of Kevlar, starting to decompose at around 500°C and producing carbon residue more than 40% of their initial weight at 700°C. In addition, these Kevlar aerogel tapes also perform well in terms of thermal insulation. When exposed to a hot plate of 300 °C, the fabric braided from these aerogel tapes show a considerable temperature drop per unit thickness as high as 120°C mm−1. It can be envisaged that the developed aerogel tapes with muscular tensile strength and their fabric products will be promising for high-temperature thermal insulation applications, such as being used as the insulation layer for thermal protective clothing

High-expression of the innate-immune related gene UNC93B1 predicts inferior outcomes in acute myeloid leukemia

Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy with dismal prognosis. Identification of better biomarkers remained a priority to improve established stratification and guide therapeutic decisions. Therefore, we extracted the RNA sequence data and clinical characteristics of AML from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression database (GTEx) to identify the key factors for prognosis. We found UNC93B1 was highly expressed in AML patients and significantly linked to poor clinical features (p < 0.05). We further validated the high expression of UNC93B1 in another independent AML cohort from GEO datasets (p < 0.001) and performed quantitative PCR of patient samples to confirm the overexpression of UNC93B1 in AML (p < 0.005). Moreover, we discovered high level of UNC93B1 was an independent prognostic factor for poorer outcome both in univariate analysis and multivariate regression (p < 0.001). Then we built a nomogram model based on UNC93B1 expression, age, FAB subtype and cytogenetic risk, the concordance index of which for predicting overall survival was 0.729 (p < 0.001). Time-dependent ROC analysis for predicting survival outcome at different time points by UNC93B1 showed the cumulative 2-year survival rate was 43.7%, and 5-year survival rate was 21.9%. The differentially expressed genes (DEGs) between two groups divided by UNC93B1 expression level were enriched in innate immune signaling and metabolic process pathway. Protein–protein interaction (PPI) network indicated four hub genes (S100A9, CCR1, MRC1 and CD1C) interacted with UNC93B1, three of which were also significantly linked to inferior outcome. Furthermore, we discovered high UNC93B1 tended to be infiltrated by innate immune cells, including Macrophages, Dendritic cells, Neutrophils, Eosinophils, and NK CD56dim cells. We also found UNC93B1 had a significantly positive correlation with CD14, CD68 and almost all Toll-like receptors. Finally, we revealed negatively correlated expression of UNC93B1 and BCL2 in AML and conjectured that high-UNC93B1 monocytic AML is more resistant to venetoclax. And we found high MCL-1 expression compensated for BCL-2 loss, thus, we proposed MCL-1 inhibitor might overcome the resistance of venetoclax in AML. Altogether, our findings demonstrated the utility of UNC93B1 as a powerful poor prognostic predictor and alternative therapeutic target