Graphene: One Material, Many Possibilities—Application Difficulties in Biological Systems

Energetic technologies, nanoelectronics, biomedicine including gene therapy, cell imaging or tissue engineering are only few from all possible applications for graphene, the thinnest known carbon configuration and a basic element for other more complicated, better discovered and widely used nanostructures such as graphite, fullerenes and carbon nanotubes. The number of researches concerning graphene applications is rising every day which proves the great interest in its unique structure and properties. Ideal pristine graphene sheet presents a flat membrane of unlimited size with no imperfections while in practice we get different flakes with irregular edges and structural defects which influence the reactivity. Nanomaterials from graphene family differ in size, shape, layer number, lateral dimension, surface chemistry and defect density causing the existence of graphene samples with various influence on biological systems. Whether graphene induces cellular stress and activates apoptosis, or on the contrary facilitates growth and differentiation of the cells depends on its structure, chemical modifications and the growth process. A certain number of in vitro studies has indicated cytotoxic effects of graphene while the other show that it is safe. The diversity of the samples and methods of the production make it impossible to establish clearly the biological impact of graphene

SOS model of overlayer induced faceting

A solid-on-solid model is proposed to describe faceting of bcc(111) metal surface induced by a metal overlayer. It is shown that the first order phase transition occurs between faceted {211} or {110} and disordered phases. The ordered phases consist of large 3-sided pyramids with {211} facets or {110} facets. It is shown that the high-temperature disordered phase has not planar bcc(111) structure but faceted disordered structure. Hysteresis effects were observed when the system was warmed above the transition temperature and then cooled down. Temperature dependence of LEED patterns for faceted and disordered phase are calculated in kinematic approximation.Comment: 8 pages, 10 figures, REVTe

A Simple Method for the Estimation of the Axial Dispersion Coefficient in Gas Flow

A simple method which is suitable for determining with reasonable precision the parameters of gas flow system has been proposed. An inverse boundary-value problem is considered. The model of gas flow with the Danckwert’s boundary conditions in a real measurement system has been analyzed and solved. The tracer technique was applied to determine axial dispersion coefficient of gas phase and Pèclet number. These parameters are commonly used to characterize the flow behavior of fluids. Axial dispersion coefficients were estimated by comparing model solution with recorded TCD signal (an inverse problem as a method for model parameter estimation) employing the Laplace transform technique. The Gaver-Stehfest algorithm for the solution of the mathematical model has been applied. The proposed model of gas show a good agreement with the experimental data. The obtained results show that under operation conditions in the studied system the flow behaviour is neither plug flow nor perfect mixing. The described method is very fast in both experimental and computational part. Simple and errorless derivation of sophisticated model formulas has been possible by application of the Computer Algebra System-type program. The program also simplifies computations. Mathematical manipulations and computations were performed using program Maple®

Oxidative Stress and Mitochondrial Activation as the Main Mechanisms Underlying Graphene Toxicity against Human Cancer Cells

Due to the development of nanotechnology graphene and graphene-based nanomaterials have attracted the most attention owing to their unique physical, chemical, and mechanical properties. Graphene can be applied in many fields among which biomedical applications especially diagnostics, cancer therapy, and drug delivery have been arousing a lot of interest. Therefore it is essential to understand better the graphene-cell interactions, especially toxicity and underlying mechanisms for proper use and development. This review presents the recent knowledge concerning graphene cytotoxicity and influence on different cancer cell lines