Investigating ancient metabolic reactions contributing to G0 quiescence survival in fission yeast S. pombe

Since all living organism rely on assimilating environmental nitrogen (N) to promote cell divisions, an efficient system to deal with N scarcity is deterministic for survival. For example, the fission yeast Schizosaccharomyces pombe (S. pombe) withstands long-term N starvation by induction of growth arrest and quiescence entry (G0 phase). In past studies 89 S. pombe genes were found to be required for survival upon G0 phase. Because these are involved in diverse intracellular functions, a clear mechanism for quiescence was difficult to assign. Therefore, we developed a BLAST-based approach to generate phylogenetic profiles and characterize evolutionary conserved metabolic reactions by comparative analysis using prokaryotic databases of Escherichia coli and Bacillus subtilis. We report fourteen proteins fulfilling this study’s homology criteria, half of which (7/14) are localized to the mitochondria. Subsequent in silico analysis suggests the involvement of two functional response mechanisms which comprise oxygen-consuming and glutamate-metabolic reactions. Among these, ∆sod2 showed abnormally low oxygen consumption upon quiescence and loss of regenerative capability. We report that the conserved mechanisms to survive N starvation comprise enzymes which are associated with the regulation of oxygen and glutamateoxoglutarate metabolism.Okinawa Institute of Science and Technology Graduate Universit

The Effects of Sensory Integrative Therapy and Functional Communication Training on Stereotypic Behavior

Three developmentally delayed individuals who exhibited self-stimulatory behaviors were exposed to sensory-integrative therapy. Prior to treatment, a Motivation Assessment Scale was completed and a functional analysis baseline was conducted to identify the maintaining variables of the self-stimulatory behavior. Each subject displayed a pattern of responding suggesting that stereotypic behaviors were maintained by automatic reinforcement. Results show that sensory-integrative therapy had no effect on self-stimulatory behaviors. The stereotypic behaviors of Subject 1 and Subject 2 were later reduced when functional communication plus response interruption was applied. The self-stimulatory behavior of Subject 3 was not affected by the implementation of functional communication procedures

Report of the 2015 NSF Cybersecurity Summit for Large Facilities and Cyberinfrastructure

This event was supported in part by the National Science Foundation under Grant Number 1234408. Any opinions, findings, and conclusions or recommendations expressed at the event or in this report are those of the authors and do not necessarily reflect the views of the National Science Foundation

Black economic development as a strategy for community development.

Massachusetts Institute of Technology. Dept. of Urban Studies and Planning. Thesis. 1970. M.C.P.Bibliography: leaves [232]-[236].M.C.P

Subsurface andesite geology and hydrothermal alteration at an exploration prospect north of Waihi

The Coromandel Volcanic Zone hosts the Hauraki Goldfield in the North Island of New Zealand. The goldfield consists of approximately 50 low sulphidation Au-Ag deposits. Situated in Coromandel Group rocks, which host 95% of the gold production in the region, the area of focus of this study is a prospect located 4 km north of the world class Martha Hill Mine. Such epithermal deposits are characterised by zones of altered rock called halos. Seven angled exploratory drill holes from the area were examined in this study. These cores were logged using high resolution photographs from a geological perspective for the first time. Samples were taken for petrographic and mineralogical analysis including X-ray diffraction analysis and for X-ray fluorescence spectroscopy. Leapfrog was used to display the spatial relationship of these results. The aim of this study was to determine the volcanic geology and alteration origins of this project. This was achieved by identifying facies as well as their spatial distribution, determining the mineralogical properties of the facies and their variations down-hole, as well as identifying the style and spatial extent of alteration. Eight new facies were identified which are hosted in andesitic rock. Three of the facies were based on visible crystal concentration which ranged from, 3% – 65% and occurred throughout the core. Fresh samples were dominated by plagioclase, with relatively minor amounts of quartz and pyroxenes and altered samples comprised of calcite+quartz assemblages with various suits of sulfides and oxides, predominantly pyrite and magnetite. The mineral assemblages and their abundances are comparable to the Waipupu Formation andesite with vast similarities to active andesite volcanoes and Late Archean examples. The rocks exhibit seriate porphyritic textures and glomeroporphyritic clots that are consistent with polybaric fractionation. The textures resulted in the host rocks innate high porosity and permeability which increases with each propagation of hydrothermal fluid. While the common resorption of quartz indicates magma mingling occurring in the magma chamber, these same resorption features as well as observed swallowtail plagioclase microlites also reflect degassing processes within the magma during ascent. Four breccia facies were described based on the concentration and orientation of breccia clasts. These facies occurred dominantly around the edge of alteration zones, which can be attributed to their relatively higher porosity. While some breccia display properties of auto breccia, hydrothermal breccia’s are dominant with all breccia likely altered to some degree. Finally, an irregular facies was described and relates to hydrothermal alteration processes exclusive to moderate and high alteration zones. Using the mineralogy data, in conjunction with stratigraphic logs, zones of alteration could be identified and designated based on intensity, describing two zones of high intensity alteration separated by a zone of moderate alteration and low alteration occurring closer to the surface. These zones give an insight into the flow of paleo fluid during alteration and can hint at the location of Au bearing veins

Changing Communities on Film: an investigation into representations of community between 1910 and 1954 in collections in the Yorkshire Film Archive

This thesis analyses representations of community in amateur and professional films from selected collections from the Yorkshire Film Archive between 1910 and 1954; a time of significant social and economic change in the region. Analysis of what appears on screen is undertaken by employing modes of textual analysis and historical and critical theories, including the Gemeinschaft and Gesellschaft, and Imagined and Constructed ideas of community on a regional and national level. The thesis first considers definitions of community and problems inherent in providing a simple and single definition. Frameworks employed to develop the analysis of film footage include methodological tools based upon prior research of archival footage and thematic, textual, and historic analysis to examine what is within the foreground and the underlying representations within the footage. Each chapter then examines the changing representations of people and the environment. However, in addition to that which exists in front of the camera the study seeks to engage with those behind the camera as being equally significant. This research uses the categorisations of amateur, professional and propaganda footage to explore and interpret the film collections. However, the boundaries for these categorisations shift over time and this fluidity in definitions is considered as part of the analysis of change over time. The linear analysis of specific collections allows for comparison and contrast between key facets of community which include class, gender, and ethnicity. The analysis argues that there is no single definition of community but that communities in Yorkshire and the Northeast change between 1910-1954. The affordability and familiarity of filmmaking grew between World War One and the Post-War period and changed people’s knowledge and exposure to filmmaking, reflecting dramatic economic and social changes. These alterations are represented on film, as the increase in filmmaking reveals changes to communities in public and private spheres

“We’re all in our private traps… and none of us can ever get out” (Psycho, Hitchcock, 1960) - How depictions of Identity and Alienation in the City vary within the films of Alfred Hitchcock

Alfred Hitchcock was a prolific director in the early to mid-twentieth century; this thesis examines how the themes of alienation, identity and the city are depicted within his films and how they reflected societal events across his British and American films, from the 1920s to 1970s. Using the research methodologies of thematic analysis to select the sample, this research examines how the themes being analysed were representative of the times in which they were shot. The thesis covers two areas: Hitchcock’s British films between 1927-1939(including Lifeboat in 1944), and American work from 1940-1960.The concluding section explores how the core themes of those films have been portrayed in later work including Taxi Driver (Scorsese, 1976) and Drive (Winding-Refn,2011).The later work is a small sample that represents these themes through the shift from Keynesian to neoliberal society and how this is reflected in the progression from Hitchcock to later work. The conclusion of this research demonstrates how in Hitchcock’s British films identity and alienation in the city represented how foreigners, racial minorities and women were alienated because the new social powers they gained were perceived to threaten the established community and male patriarchy. Conversely, in his American work one can read that Hitchcock depicts society from a psychoanalytic perspective, as it was of rising interest in these times through how people either performed multiple identities or were psychologically alienated from themselves through self-estrangement. In these films the interest in psychology peaked as well as Cold War paranoia, which increased people’s fears of masked and multiple identities. Finally, in the later work it appears that alienation occurs through the lack of norms and bonds within society. Therefore, the research demonstrates how alienation, identity and the city’s cinematic representations differ greatly, and their depiction is strongly influenced by what events occurred in each context

ASPECTOS QUÍMICOS DOS FRUTOS DE Prunus myrtifolia (L.) Urban, (ROSACEAE) – ALIMENTO DE ALGUMAS AVES SILVESTRES

Frutos maduros de Prunus myrtifolia, pertencente à família Rosaceae, são procurados como alimento por algumas espécies de aves silvestres, no Núcleo Cunha do Parque Estadual da Serra do Mar – município de Cunha, Estado de São Paulo. Com o objetivo de avaliar alguns componentes químicos e valor calórico, amostras coletadas em agosto de 2005 foram analisadas quimicamente nos laboratórios dos Institutos Florestal e Adolfo Lutz e Instituto de Química da Universidade de São Paulo. Os resultados mostraram a presença de glicosídeo cianogenético tanto na casca como nas sementes, além de umidade (38,52%), lipídios (0,62%), proteínas (5,38%) e açúcares totais (46,27%). Como elementos inorgânicos (16), predominaram, tanto na casca como nas sementes, os elementos K (0,54 e 0,45%) e Ca (0,51 e 0,14%), respectivamente, e em relação aos micronutrientes, destacaram-se o Mg (1676,80 e 843,50 ppm), P (708,50 e 892,10 ppm) e S (351,50 e 321,50 ppm), respectivamente

“It Happens to Girls All the Time”: Examining Sexual Assault Survivors’ Reasons for Not Using Campus Supports

Sexual assault is a prevalent problem in higher education, and despite the increasing availability of formal supports on college campuses, few sexual assault survivors use them. Experiencing sexual assault can have devastating consequences on survivors’ psychological and educational wellbeing, which may intensify if survivors do not receive adequate care. Drawing from existing theoretical frameworks and empirical research, this study used a mixed methodological approach to examine why survivors did not use three key campus supports—the Title IX Office, the sexual assault center, and housing staff—and if these reasons differed across the three supports. Using data from 284 women who experienced sexual assault in college, our qualitative findings identified four overarching themes, including logistical issues (e.g., lacking time and knowledge), feelings, beliefs, and responses that made it seem unacceptable to use campus supports, judgments about the appropriateness of the support, and alternative methods of coping. Quantitative findings revealed that survivors’ reasons for not seeking help differed across supports. Collectively, our findings suggest that community norms and institutional policies can make it challenging for survivors to use campus supports. We propose several suggestions for institutional change (e.g., taking a stronger stance against “less serious” forms of sexual assault, reducing a quasi‐criminal justice approach to investigation and adjudication, limiting mandated reporting).HighlightsIdentifies reasons why college students who experience sexual assault do not use formal sources of support on campus.Examines how reasons for not using formal supports differ across sources of support: Title IX Office, sexual assault center, and housing staff.Provides an in‐depth, contextual understanding of sexual assault survivors’ use and avoidance of campus supports in the wake of substantial policy change.Contributes recommendations for institutional policy and practice based on findings, including taking a stronger stance against “less serious” forms of sexual assault, reducing a quasi‐criminal justice approach to investigation and adjudication, and limiting mandated reporting.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/136479/1/ajcp12126.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/136479/2/ajcp12126_am.pd