3,042 research outputs found

    Detection and immunolabelling of peroxisomal proteins

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    This is the author accepted manuscript. The final version is available from Humana Press via the DOI in this record.Peroxisomes are essential organelles in mammals which contribute to cellular lipid metabolism and redox homeostasis. The spectrum of their functions in human health and disease is far from being complete, and unexpected and novel roles of peroxisomes are being discovered. To date, those include novel biological roles in anti-viral defence, as intracellular signalling platforms and as protective organelles in sensory cells. Furthermore, peroxisomes are part of a complex network of interacting subcellular compartments which involves metabolic cooperation, cross-talk and membrane contacts. As potentially novel peroxisomal proteins are continuously discovered, there is great interest in the verification of their peroxisomal localisation. Here, we present protocols used successfully in our laboratory for the detection and immunolabelling of peroxisomal proteins in cultured mammalian cells. We present immunofluorescence and fluorescence-based techniques as well as reagents to determine peroxisome-specific targeting and localisation of candidate proteins.We would like to thank A. Manner for providing images for Fig. 1D. This work was supported by the Marie Curie Initial Training Network (ITN) action (FP7-2012-PERFUME-316723) and the Biotechnology and Biological Sciences Research Council (BB/K006231/1; BB/N01541X/1)

    State of the Science Conference: Abstract and Executive Summary

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    This document provides an overview of research findings presented and audience response at the “state-the-science” conference conducted by Cornell University October 22-23, 2013 in Washington, D.C. on the topic of Innovative Research on Employment Practices: Improving Employment for People with Disabilities

    Vertical supports of the PV Wendelstein 7-X

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    Unloosing the Gordian knot of peroxisome formation

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    This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this recordPeroxisome biogenesis is governed by molecular machineries, which are either unique to peroxisomes or are partially shared with mitochondria. As peroxisomes have important protective functions in the cell, modulation of their number is important for human health and disease. Significant progress has been made towards our understanding of the mechanisms of peroxisome formation, revealing a remarkable plasticity of the peroxisome biogenesis pathway. Here we discuss most recent findings with particular focus on peroxisome formation in mammalian cells.This work was supported by the Biotechnology and Biological Sciences Research Council (BB/K006231/1, BB/N01541X/1 to M.S.)

    The peroxisome: still a mysterious organelle.

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    More than half a century of research on peroxisomes has revealed unique features of this ubiquitous subcellular organelle, which have often been in disagreement with existing dogmas in cell biology. About 50 peroxisomal enzymes have so far been identified, which contribute to several crucial metabolic processes such as beta-oxidation of fatty acids, biosynthesis of ether phospholipids and metabolism of reactive oxygen species, and render peroxisomes indispensable for human health and development. It became obvious that peroxisomes are highly dynamic organelles that rapidly assemble, multiply and degrade in response to metabolic needs. However, many aspects of peroxisome biology are still mysterious. This review addresses recent exciting discoveries on the biogenesis, formation and degradation of peroxisomes, on peroxisomal dynamics and division, as well as on the interaction and cross talk of peroxisomes with other subcellular compartments. Furthermore, recent advances on the role of peroxisomes in medicine and in the identification of novel peroxisomal proteins are discussed.Supported by the German Research Foundation (DFG) (SCHR 518/6-1) and the Portuguese Foundation for Science and Technology (FCT) (PTDC/BIA-BCM/71932/2006)

    Conceptual design for measuring soil management sustainability

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    Soils are the nexus of food, energy and water which illustrates the need for a holistic approach in sustainable soil management. The search for relevant bioindicators of soil sustainability has led to a huge output of studies recently, but yet a proper parameter or a set of parameters has not been identified. Resilience is often promoted to be a boundary concept to integrate social and natural dimensions of sustainability. Therefore, resilience is a promising parameter when it comes to measuring sustainability of soil management practices, since it reflects both its highly interlinked ecological and management components. To include both of these two interlinked components, the whole concept of soil ecosystem functioning needs to be reconsidered. We will present a modified concept of soil functioning cycles within the three dimensions of potential, connectedness and resilience. Additionally, we present a first methodological approach of how to measure resilience by the maximum ecological performance (MEP), using the multi-omics approach. We will present resilience as a key element of an adaptive management scheme, to also meet the challenge of deriving information about the link between soil biodiversity and soil multifunctionality
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