Selection of UUV Type ROV Equipment and Cooperation System with USV "Edredon" in Protection Tasks of Ports and Critical Objects

The article presents some of the problems associated with the use of an unmanned underwater vehicle type ROV (Remotely Operated Vehicle) to cooperate with the USV (Unmanned Surface Vehicle) "Edredon" carrying out tasks related to the perimetric protection of seaports infrastructure and critical objects. The system remote control of the underwater vehicle, requires the appropriate structure of the system ensuring the cooperation of both vehicles and the selection of the special apparatus mounted on the ROV. The tasks carried out by the both vehicles have a very high impact on the hardware solutions and cooperation of USV with an unmanned underwater vehicle

Communication System Between the ROV and the USV’s “Edredon” Control Post

One of the tasks carried out by the operator from the Command Post (land or ship), who controls the unmanned surface vehicle (USV „Edredon”) is the protection of critical marine objects (ports, ships, container terminals, etc.). This task is often carried out by using a remote operated vehicle type ROV (unmanned underwater vehicle). This vehicle is transported on board the USV at the site of the planned underwater reconnaissance area. When USV reach the scheduled location, the USV operator switches to remote control of the ROV vehicle. The article discusses solving the problem of remote control of the underwater vehicle ROV from Command Post. The operator uses the Launch and Recovery (L&R) system on the USV to control the ROV ditching process from the USV deck. It then controls the movement of the ROV vehicle under water (images from cameras and data from sensors placed on the vehicle ROV are transmitted via USV to the Command Post). After completing the task, the ROV vehicle is lifted aboard the USV, which carries out the next task

Benchmarking High Performance Architectures With Natural Language Processing Algorithms

Natural Language Processing algorithms are resource demanding, especially when tuning toinflective language like Polish is needed. The paper presents time and memory requirementsof part of speech tagging and clustering algorithms applied to two corpora of the Polishlanguage. The algorithms are benchmarked on three high performance platforms of differentarchitectures. Additionally sequential versions and OpenMP implementations of clusteringalgorithms were compared

Mobile Social Networks For Live Meetings

In this article, we present an idea of combining social networking websites andmodern mobile devices abilities to transfer social networking activity to a higherlevel. Nowadays, these devices and websites are used to offer ability of remotecommunication (phone calls, message exchange etc.), which potentially can beused to notify people about meetings in the real world. Since the current socialnetwork models do not provide enough information for such notification (socialnetworking websites are examples of social networks) a new social network modelthat will be suitable for the above mentioned application is proposed and a newsocial platform that base on mobile devices is introduced. This platform cannotify users when their friends are nearby. The paper presents the model andthe simulation that verifies the approach

Bio-orthogonal site-selective labelling of carbohydrates and proteins

Carbohydrates and proteins represent two large groups of biomolecules which are tremendously important for biological processes in health and disease state. Although protein-structures are encoded in the genome, cellular glycan structures are template independent and can only be addressed in an indirect manner. The development of metabolic oligosaccharide engineering (MOE) gave rise to new methods to study carbohydrate structures in the context of different disease settings and in different organisms. While in many cases mannose derivatives are used to study the sialic acid structures in cancer cells, this work presents the results on the metabolic incorporation of galactose derivatives into cell membrane glycans of human hepatic cells. Three unnatural galactose derivatives containing terminal alkene groups in C2 or C6 position were synthesized and their reaction rates in inverse electron demand Diels Alder reactions (iEDDA) were evaluated, by using a high-throughput screening method in 96-well plates. It was shown that none of the developed galactose derivatives exhibit any cell toxic effect in HepG2 or Huh7 cell lines. Furthermore, all monosaccharides could be successfully incorporated in cell membrane glycan structures of both cell lines and the localization on the cell membrane was confirmed by co-localization with a plasma membrane dye. After developing this incorporation and labeling strategy of unnatural galactose derivatives in the cell membrane of human hepatic cells, the change in incorporation during an infection of these cells with Plasmodium berghei sporozoites was investigated. By using different techniques, such as confocal microscopy, flow cytometry and imaging flow cytometry, only a small trend for an increased uptake of the unnatural galactose derivative in P. berghei infected cells was observed. To explain this result, the pathway for the diffusion of the unnatural galactose derivative was determined. The application of specific and non-specific inhibitors for the glucose transporter GLUT1 revealed that this transporter is involved in the delivery of galactose derivatives into cultured cells. The enhanced translocation of this transporter to the surface of infected hepatic cells explains the observed tendency for an increased incorporation of the unnatural galactose derivative in these cells. Apart from cell studies, MOE was applied for the first time to study a possible transfer of galactose monosaccharides from the mosquito host to the parasite. Biosynthetic pathways for glycan assembly in the parasite are poorly understood. Suggestions on the participation of the mosquito host in some of these pathways, led to the idea to apply MOE in this situation. It was possible to show an uptake of the presented galactose derivatives by the mosquito but only reduced transfer to the parasite seems to occur. In addition to the development of monofunctional galactose derivatives, also a bifunctional derivative containing two orthogonal reporter groups was synthesized. However, so far it was not possible to achieve a metabolic incorporation or labeling of this derivative on cell membrane glycans. After developing cellular tools to study carbohydrate structures, a site-selective method for protein modification was generated, to be used for the development of new glycoconjugate vaccine candidates. By introducing selectively two dehydroalanine residues in place of the disulfide bond C186-C201 of the immunogenic protein CRM197, a new chemical moiety for the conjugation of carbohydrate antigens was obtained. It was shown that these moieties can be used for the selective introduction of polysaccharide antigens from group B Streptococcus (GBS) or Streptococcus pneumoniae. Both types of glycoconjugates could be synthesized and first trials on the purification methods were undertaken. This concept will be developed further for future vaccine candidates. Finally, a synthetic method was developed which could facilitate the synthesis of defined antigenic oligosaccharide structures. This method uses the thiophilic promoter O-mesitylenesulfonylhydroxylamine (MSH) for the activation of thioglycoside donors. It was demonstrated that different thioglycoside donors are activated with different kinetics, depending on the presented protecting groups or the anomeric leaving group. Apart from applying the developed activation method for the synthesis of several glycosylation products, the sequential activation of S-alkyl before S-phenyl anomeric groups was shown during the synthesis of a model trisaccharide. Overall, bio-orthogonal methods were developed and applied for the investigation of carbohydrate structures in the context of malaria disease, and for the site-selective modification of protein carriers during the development of glycoconjugate vaccine candidates

Comparison of Latent Semantic Analysis and Probabilistic Latent Semantic Analysis for Documents Clustering

In this paper we compare usefulness of statistical techniques of dimensionality reduction for improving clustering of documents in Polish. We start with partitional and agglomerative algorithms applied to Vector Space Model. Then we investigate two transformations: Latent Semantic Analysis and Probabilistic Latent Semantic Analysis. The obtained results showed advantage of Latent Semantic Analysis technique over probabilistic model. We also analyse time and memory consumption aspects of these transformations and present runtime details for IBM BladeCenter HS21 machine

Comparison of Information Representation Formalisms for Scalable File Agnostic Information Infrastructures

In the early days of computing, files where just a natural way of storing information -- which reflected the way one would file their punch cards in a cabinet drawer. Unfortunately, the requirement to fragment information into such chunks, is a huge bottleneck for the evolution of global information space that the Internet has become. The concept of file causes several problems including unnatural clustering of information, unnecessary replication of data and very expensive information discovery in distributed computing environments. The overall goal of this work is to design an architecture enabling new era in computing and networking -- a computing infrastructure without the concept of file. Files are seen by many specialists as one of the main bottlenecks of modern IT systems evolution. This is mostly due to a very unnatural fragmentation of information into chunks which are easier to manage by operating systems but much more difficult for information processing tools and eventually by humans themselves

A Sweet Galactose Transfer: Metabolic Oligosaccharide Engineering as a Tool To Study Glycans in Plasmodium Infection.

The introduction of chemical reporter groups into glycan structures through metabolic oligosaccharide engineering (MOE) followed by bio-orthogonal ligation is an important tool to study glycosylation. We show the incorporation of synthetic galactose derivatives that bear terminal alkene groups in hepatic cells, with and without infection by Plasmodium berghei parasites, the causative agent of malaria. Additionally, we demonstrated the contribution of GLUT1 to the transport of these galactose derivatives, and observed a consistent increase in the uptake of these compounds going from naïve to P. berghei-infected cells. Finally, we used MOE to study the interplay between Plasmodium parasites and their mosquito hosts, to reveal a possible transfer of galactose building blocks from the latter to the former. This strategy has the potential to provide new insights into Plasmodium glycobiology as well as for the identification and characterization of key glycan structures for further vaccine development

Essential trace (Zn, Cu, Mn) and toxic (Cd, Pb, Cr) elements in the liver of birds from Eastern Poland

We have focused our study on the concentrations of essential heavy metals (Zn, Cu and Mn) and non-essential trace metals (Pb, Cd and Cr) in the livers of birds from Eastern Poland. The largest mean amount of Zn - as much as 279 mg/kg dry mass (d.m.) - was found in mute swans. However, only in one of the analysed buzzard specimens the concentration of Zn, found to be 664 mg/kg d.m., exceeded the level indicative of poisoning for this element. Birds specializing in catching rodents accumulated Mn in their livers in a very narrow range of concentrations, around 5.0 mg/kg d.m. on average. The range of mean Mn concentrations (around 6.5 mg/kg d.m.) was also found to be narrow for piscivorous birds. The highest mean levels of Pb were found in mute swans (2.7 mg/kg d.m.), and the highest levels of Cd (2.0 mg/kg d.m.) for rooks. Concentrations of total Cr above detection level were found in 22 specimens (53.7%), and concentration values were highest for rooks. Analyses showed that the concentrations of biogenic elements did not exceed the levels indicative of poisoning (except in one specimen). The study demonstrated that lead shots remain a hazard to water ecosystems. Pb, Cd and Cr levels in the livers of omnivorous and piscivorous species indicate the permanent presence of these elements in the environment and may confirm the thesis about the growing role of electronic waste, including metallic e-waste, in the emission of the total amount of contamination with these elements