19 research outputs found

    Bovine leukemia virus in Antioquia dairies: molecular prevalence, circulating genotypes and factors associated with its circulation.

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    ilustraciones, diagramasEl virus de la leucosis bovina (BLV) es un retrovirus que afecta los sistemas de producci贸n de lecher铆a especializada en todo el mundo, relacionado con menor producci贸n de leche y reducci贸n en la vida de los animales infectados. Se han identificado once genotipos del virus circulando y actualmente no existe tratamiento ni vacuna para la enfermedad por lo que las estrategias de control son esenciales. En Colombia la lecher铆a contribuye a la econom铆a y sustento alimentario del pa铆s, Antioquia es departamento mayor productor. Para establecer el estado actual del BLV en los sistemas productivos de Antioquia se seleccionaron 575 bovinos pertenecientes a 53 hatos de las regiones Norte, Oriente y Valle de Aburr谩, de diferentes razas y edades. Se hizo la caracterizaci贸n de los sistemas productivos, se estudi贸 la prevalencia molecular mediante una PCR anidada del gen env de BLV, resultando un 17,04% en animales y 75,5% en hatos y por un estudio filogen茅tico del gen tax estableciendo la presencia 煤nicamente del genotipo 1. Adem谩s, se determinaron factores de riesgo y de protecci贸n asociados a la infecci贸n, encontrando que pr谩cticas como el orde帽o mec谩nico y la inseminaci贸n artificial (con semen sin conocer estatus microbiol贸gico) suponen un riesgo de infecci贸n con BLV y como factores de protecci贸n se encontraron de desinfectantes alcoholes y agentes alquilantes y la implementaci贸n de sistemas de pediluvio. Los resultados encontrados en este estudio muestran la necesidad de plantear un programa de prevenci贸n y educaci贸n para controlar la infecci贸n por BLV y minimizar las p茅rdidas econ贸micas que supone la infecci贸n. (Texto tomado de la fuente)Gobernaci贸n de AntioquiaCOLANTAMaestr铆aMag铆ster en Ciencias - Biotecnolog铆aDise帽o experimental y muestraBiotecnolog铆a animal脕rea Curricular Biotecnolog铆

    Prevalencia molecular del Virus de Leucosis Bovina en lecher铆as especializadas del departamento de Antioquia, Colombia

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    Dairy production systems are a sensitive sector of the primary economy frequently affected by pathogens that negatively impact production parameters, the bovine leukemia virus (BLV) one of these. In this study, the molecular prevalence of BLV was determined in the specialized dairy sector of Antioquia using the viral marker of the envelope gene (env). Blood samples were taken from 575 bovines from specialized dairies in Antioquia distributed in 53 herds and located in the three specialized milk production areas of Antioquia (north, east, and Valle de Aburr谩). DNA extraction was performed by salting out, and a nested PCR was performed to detect the env gene. The products were visualized on a 2% agarose gel with GelRed as an intercalator. A molecular prevalence of BLV of 17.0% in animals and 71.7% in herds were found, being Valle de Aburr谩 the area where the highest rate of positive animals was obtained (21.1%), unlike the northern area with the lowest rate (15.6%). The molecular prevalence of BLV in this study is lower than that of previous studies in the department, which ranged between 47 and 73%, and this may be associated with factors of breed resistance, the age of the animals, or management practices in the herds. These results can contribute to creating BLV control strategies and optimizing milk production in the department of Antioquia, being relevant to paying attention to the behavior of this pathogen under different production system conditions.Los sistemas de producci贸n de leche son un sector sensible de la econom铆a primaria, vi茅ndose frecuentemente afectados por pat贸genos que impactan negativamente par谩metros productivos, siendo el virus de la leucosis bovina (BLV) uno de estos. En este estudio, se determin贸 la prevalencia molecular a BLV en la lecher铆a especializada de Antioquia mediante el marcador viral del gen de la envoltura (env). Se tom贸 muestra de sangre de 575 bovinos de lecher铆as especializadas de Antioquia distribuidos en 53 hatos, ubicados en las tres zonas de producci贸n de leche especializada de Antioquia (norte, oriente y Valle de Aburr谩). Se realiz贸 extracci贸n de ADN por Salting out y se realiz贸 una PCR anidada para detecci贸n del gen env. Los productos se visualizaron en un gel de agarosa al 2% con GelRed como intercalante. Se encontr贸 una prevalencia molecular a BLV del 17,0% en animales y 71,7% en hatos, siendo el Valle de Aburr谩 la zona donde se obtuvo la mayor tasa de animales positivos (21,1%), a diferencia del norte que tuvo la m谩s baja (15,6%). Se hall贸 que la prevalencia molecular a BLV de este estudio es menor a la de investigaciones previas en el departamento, la cual se encontraba entre el 47 y 73%, y esto puede estar asociado a factores de resistencia raciales, edad de los animales o las pr谩cticas de manejo en los hatos. Estos resultados pueden contribuir a crear estrategias de control del BLV y optimizar la producci贸n lechera en el departamento de Antioquia, siendo relevante poner atenci贸n al comportamiento de este pat贸geno bajo las diferentes condiciones que tienen los sistemas de producci贸n

    Incidencia de delirium en pacientes cr铆ticos pedi谩tricos: estudio de cohorte prospectiva

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    Delirium is a common complication among critically ill pediatric patients. Objective: To estimate the incidence of delirium in patients admitted to a pediatric critical care unit. Methodology: An observational, descriptive, follow-up study of a prospective cohort of patients under 16 years of age, who were admitted consecutively to the pediatric critical care unit of a university hospital in the Province of Buenos Aires, Argentina between December 2018 and December 2019. Cornell scale, in its validated version in Spanish, for the systematized screening of delirium, carried out by nursing personnel at 6 am and 6 pm. The incidence of delirium was estimated as cumulative incidence and incidence density. Ethical considerations: The study was approved by the Independent Evaluation Committee. Results: Data from 116 critical pediatric patients were analyzed. The median age was 2 years (IQR 0.3 - 7) and 52.59% were male. The cumulative incidence of delirium was 52.58% (95% CI 43.10-61.93) in 12 months and the incidence density was 10.28 / 100 patient days. The incidence rate of delirium was lower in men (IR 0.59; 95% CI 0.34-1.02; p = 0.04). Physical restriction showed a significant association with delirium (RR 2.97; 95% CI 1.14 - 6.55; p = 0.01). Likewise, the longer the hospitalization time, a higher incidence of delirium was observed (p <0.0001). Discussion: We found a high incidence of delirium in our cohort, with a higher risk of development in the first days of hospitalization. The findings provide useful information on delirium in a cohort of Argentine patients; they help to highlight the importance of carrying out systematic screening of delirium by the nursing team.El delirium es una complicaci贸n frecuente entre los pacientes cr铆ticos pedi谩tricos. Objetivo: Estimar la incidencia de delirium en pacientes internados en unidad de cuidado cr铆ticos pedi谩tricos. Material y m茅todos: Estudio observacional, descriptivo, de seguimiento de una cohorte prospectiva de pacientes menores de 16 a帽os, que ingresaron en forma consecutiva en la unidad de cuidados cr铆ticos pedi谩tricos de un hospital en Argentina.. Se utiliz贸 la escala de Cornell, en su versi贸n validada en espa帽ol, para el cribado sistematizado de delirium, realizado por personal de enfermer铆a a las 6 am y a las 6 pm. La incidencia de delirium fue estimada como incidencia acumulada y densidad de incidencia. El estudio fue aprobado por Comit茅 Independiente de Evaluaci贸n. Resultados: Se analizaron datos de 116 pacientes cr铆ticos pedi谩tricos. La mediana de edad fue de 2 a帽os (RIC 0.3 - 7) y 52,59% eran del g茅nero masculino. La incidencia acumulada de delirium fue de 52,58% (IC95% 43,10-61,93) en 12 meses y la densidad de incidencia fue de 10,28/100 pacientes d铆as. La tasa de incidencia de delirium fue menor en los varones (IR 0,59; IC95% 0,34-1,02; p=0,04). La restricci贸n f铆sica mostr贸 asociaci贸n significativa con delirium (RR 2,97; IC95% 1,14 - 6,55; p=0,01). Asimismo, a mayor tiempo de estancia en UCIP se observ贸 mayor incidencia de delirium (p<0,0001). Discusi贸n: Encontramos en nuestra cohorte una incidencia de delirium elevada, con mayor riesgo de desarrollo en los primeros d铆as de internamiento. Los hallazgos sostienen la importancia de la realizaci贸n del cribado sistematizado de delirium por el equipo de enfermer铆a

    A recombinant O-polysaccharide-protein conjugate approach to develop highly specific monoclonal antibodies to Shiga toxin-producing Escherichia coli O157 and O145 serogroups

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    Shiga toxin-producing Escherichia coli (STEC) is the major etiologic agent of hemolytic-uremic syndrome (HUS). The high rate of HUS emphasizes the urgency for the implementation of primary prevention strategies to reduce its public health impact. Argentina shows the highest rate of HUS worldwide, being E. coli O157 the predominant STEC-associated HUS serogroup (>70%), followed by E. coli O145 (>9%). To specifically detect these serogroups we aimed at developing highly specific monoclonal antibodies (mAbs) against the O-polysaccharide (O-PS) section of the lipopolysaccharide (LPS) of the dominant STEC-associated HUS serogroups in Argentina. The development of hybridomas secreting mAbs against O157 or O145 was carried out through a combined immunization strategy, involving adjuvated-bacterial immunizations followed by immunizations with recombinant O-PS-protein conjugates. We selected hybridoma clones that specifically recognized the engineered O-PS-protein conjugates of O157 or O145 serogroups. Indirect ELISA of heat-killed bacteria showed specific binding to O157 or O145 serogroups, respectively, while no cross-reactivity with other epidemiological important STEC strains, Brucella abortus, Salmonella group N or Yersinia enterocolitica O9 was observed. Western blot analysis showed specific recognition of the sought O-PS section of the LPS by all mAbs. Finally, the ability of the developed mAbs to bind the surface of whole bacteria cells was confirmed by flow cytometry, confocal microscopy and agglutination assays, indicating that these mAbs present an exceptional degree of specificity and relative affinity in the detection and identification of E. coli O157 and O145 serogroups. These mAbs may be of significant value for clinical diagnosis and food quality control applications. Thus, engineered O-PS specific moieties contained in the recombinant glycoconjugates used for combined immunization and hybridoma selection are an invaluable resource for the development of highly specific mAbs.Fil: Castillo, Daniela Susana. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Rey Serantes, Diego A.. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Melli, Luciano Jorge. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Ciocchini, Andres Eduardo. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Ugalde, Juan Esteban. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Comerci, Diego Jos茅. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; ArgentinaFil: Cassola, Alejandro Carlos. Consejo Nacional de Investigaciones Cient铆ficas y T茅cnicas. Centro Cient铆fico Tecnol贸gico Conicet - La Plata. Instituto de Investigaciones Biotecnol贸gicas. Universidad Nacional de San Mart铆n. Instituto de Investigaciones Biotecnol贸gicas; Argentin

    Exacerbation and appearance of dermatosis during COVID-19 quarantine period in Bogot谩, Colombia: a descriptive study of the psychological impairments

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    Introduction: Quarantine established in several countries by the new coronavirus pandemic, dramatically changed our lifestyle, affecting quality of life, skin, and psyche. We described the skin diseases that initiated or were exacerbated in quarantine and assessed the impact on quality of life and the possible presence of anxiety. Materials and methods: We performed a descriptive study from April 2020 to August 2020, assessing two questionnaires: Dermatology Life Quality Index (DLQI) and Hospital Anxiety and Depression Scale (HADS-A) in a dermatologic center. Variables analyzed were age, sex, onset or exacerbation of skin disease, DLQI and HADS-A scores. We used relative and absolute frequencies to describe patients. We collected data using Microsoft Excel and analyzed with SPSS. Results: Of 124 patients, 85 (69%) were women. Acne was the most frequent dermatosis with 18,5% (n = 23), followed by contact dermatitis with 22 patients (17,7%). HADS-A results showed that 24.2% of patients had psychological distress. DLQI most frequent results had a small effect in life (29.8%; n = 37). Patients with psoriasis and eczematous diseases showed a major impact on their quality of life. Conclusions: Quarantine has been related to exacerbation of pre-existent dermatologic conditions and the onset of skin affections. Lifestyle changes influenced the occurrence of acne and contact dermatitis. DLQI and HADS results did not show an important impact in patients鈥 quality of life. Further epidemiologic studies are needed to determine whether there is a causal relationship between quarantine and these dermatologic conditions

    Surface staining of O157 and O145 STEC strains with O157 and O145 mAbs.

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    <p><b>(A-B)</b> Binding of O157 3F10 and O145 4C8 mAbs to <i>E</i>.<i>coli</i> O157 <b>(A)</b> or <i>E</i>.<i>coli</i> O145 <b>(B)</b> heat-killed bacteria assessed by flow cytometry. An irrelevant isotype-matched murine mAb or only secondary Ab were used as controls. <b>(C-D)</b> Immunostaining of <i>E</i>.<i>coli</i> O157 and O145 with O157 3F10 <b>(C)</b> and O145 4C8 <b>(D)</b> mAbs, visualized by confocal microscopy. Scale bar, 3 渭m.</p

    Specificity of O157 and O145 mAbs towards STEC strains by iELISA.

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    <p>iELISA of representative STEC strains of serogroups O157, O145, O121, O111, O104, O103, O45 and O26 <b>(A-B)</b>, and iELISA of <i>B</i>. <i>abortus</i> 2308, <i>S</i>. Urbana, <i>Y</i>. <i>enterocolitica</i> O9 and <i>E</i>. <i>coli</i> O157 <b>(C)</b> or <i>E</i>. <i>coli</i> O145 <b>(D)</b>, using O157 1E10, 3F10 and 10G2 mAbs <b>(A,C)</b> or O145 2H6, 4C8 and 4E6 mAbs <b>(B,D)</b>. In <b>(A-B)</b> non-pathogenic <i>E</i>. <i>coli</i> DH5伪 strain was used as a negative control, and in <b>(C)</b> O9 M84 mAb was used as <i>B</i>. <i>abortus</i> and <i>Y</i>. <i>enterocolitica</i> O9 positive control. Each point of the curve represents the mean卤SD of three sample replicates.</p

    Agglutination assay of STEC strains with O157 and O145 mAbs.

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    <p>Detection of O157 <b>(A)</b> and O145 <b>(B)</b> antigens in representative STEC strains of serogroups O157, O145, O121, O111, O104, O103, O45 and O26 by agglutination assays using O157 3F10 mAb or O157 mouse antisera <b>(A)</b>, or O145 4C8 mAb or O145 mouse antisera <b>(B)</b>. AS: antisera.</p

    Relative affinity of mAbs towards O157-AcrA and O145-AcrA by glyco-iELISA.

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    <p>Standard curves of O157-AcrA <b>(A)</b> or O145-AcrA <b>(B)</b> detected by glyco-iELISA with the use of mAbs O157 1E10, 3F10 and 10G2 (3:8 hybridoma supernatant dilution) <b>(A)</b> or mAbs O145 2H6, 4C8 and 4E6 (1:2 hybridoma supernatant dilution) <b>(B)</b>. Each point of the curve represents the mean卤SD of two sample replicates. IC50 values of the mAbs are indicated.</p
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