825 research outputs found
Chemical investigation of light induced DNA bipyrimidine damage and repair
In all organisms, genetic information is stored in DNA and RNA. Both of these macromolecules
are damaged by many exogenous and endogenous events, with UV irradiation being one of the
major sources of damage. The major photolesions formed are the cyclobutane pyrimidine dimers
(CPD), pyrimidine–pyrimidone-(6-4)-photoproducts, Dewar valence isomers and, for dehydrated
spore DNA, 5-(a-thyminyl)-5,6-dihydrothymine (SP). In order to be able to investigate how
nature’s repair and tolerance mechanisms protect the integrity of genetic information,
oligonucleotides containing sequence and site-specific UV lesions are essential. This tutorial review
provides an overview of synthetic procedures by which these oligonucleotides can be generated,
either through phosphoramidite chemistry or direct irradiation of DNA. Moreover, a brief
summary on their usage in analysing repair and tolerance processes as well as their biological
effects is provided
Sunlight-Induced DNA Lesions. Lesion Structure, Mutation Characteristics and Repair
The UV part of sunlight is known to induce a variety of genome defects. These lesions are the major cause of skin cancer development. In order to counter such toxic effects cells have developed a number of sophisticated DNA repair systems, like nucleotide excision repair and photoreactivation. The repair machinery is able to specifically recognize sunlight-induced DNA lesions and to subsequently remove this damage. Malfunctioning repair systems are responsible for the three rare genetic diseases Xeroderma pigmentosum, Cockayne's syndrome, and trichothiodystrophy. In this review article, the structure of the major sunlight-induced lesions will be discussed. An overview of the two major repair mechanisms, photoreactivation and excision repair, is given, and the effects of the DNA lesions on the p53 gene and on tumor genesis are discussed
The relation between the poetic concept and autobiographical memory in the works of Thomas Wolfe
Thomas Wolfe\u27s literary output includes four long novels and a vast number of short stories and poems. In addition to this he left a great deal of manuscript which is still in the process of being examined and assorted. His four long novels have drawn the greatest attention, for it is in these that he has done his finest and most provocative work. In each one Wolfe is the central figure, and through his eyes we are allowed to see the world as he saw it. It is because of this that the charges of egotism, paranoia tendencies, genius, immaturity, and plain mediocrity have been hurled at him. From the statement of Sinclair Lewis that Wolfe might live to be the greatest writer that America has yet produced, to Canby\u27s I think that this novel Of Time and the Riverlike many fiery and ambitious American books ... is an artistic flop, Wolfe has been analyzed and interpreted by critics, near critics, and uncritical sentiment. The most consistent criticism leveled at him is that whatever success he may have attained is due to autobiographical memory, and that if he laid aside the tools of subjectivity, his work would have only an ordinary prosaic quality.
It is the purpose of this study to demonstrate that Wolfe\u27s accomplishments were due not to autobiographical memory but instead to a supreme intensity of poetic feeling and tremendous scope of power and imagery--to show that Thomas Wolfe was an artist who was abundantly rich in word range, a master of characterization and of creative imagination, thereby to refute the claim of those who narrow his potentialities merely to those of an autobiographical nature
Mitogenic signaling by Gq/11-coupled receptors
By binding to their cognate GPCRs, many potent
mitogens such as neuropeptides, angiotensin II or
lysophosphatidic acid stimulate cell proliferation via engaging
the ERK/MAPK cascade. As mentioned before, agonists stimulating
Gq/11-coupled receptors activate PLCb isoforms thereby
activating PKCs and elevating [Ca2+]i. These two second
messengers represent key molecules for coupling Gq/11 proteins
to the ERK/MAPK cascade. In this work, by means of GnRH in
gonadotropic aT3-1 cells and galanin or bradykinin in SCLC
cells, different aspects of Gq/11-dependent mitogenic signaling
pathways were revealed. Our findings together with previous
reports underline the notion that signaling pathways emanating
from Gq/11-coupled receptors are tightly regulated in a cell-
and receptor-specific manner
Tissue distribution of 5-hydroxymethylcytosine and search for active demethylation intermediates.
5-Hydroxymethylcytosine (hmC) was recently detected as the sixth base in mammalian tissue at so far controversial levels. The function of the modified base is currently unknown, but it is certain that the base is generated from 5-methylcytosine (mC). This fuels the hypothesis that it represents an intermediate of an active demethylation process, which could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. Here, we use an ultra-sensitive and accurate isotope based LC-MS method to precisely determine the levels of hmC in various mouse tissues and we searched for 5-formylcytosine (fC), 5-carboxylcytosine (caC), and 5-hydroxymethyluracil (hmU) as putative active demethylation intermediates. Our data suggest that an active oxidative mC demethylation pathway is unlikely to occur. Additionally, we show using HPLC-MS analysis and immunohistochemistry that hmC is present in all tissues and cell types with highest concentrations in neuronal cells of the CNS
Direct and Base Excision Repair-Mediated Regulation of a GC-Rich cis -Element in Response to 5-Formylcytosine and 5-Carboxycytosine
Stepwise oxidation of the epigenetic mark 5-methylcytosine and base excision repair (BER) of the resulting 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) may provide a mechanism for reactivation of epigenetically silenced genes; however, the functions of 5-fC and 5-caC at defined gene elements are scarcely explored. We analyzed the expression of reporter constructs containing either 2′-deoxy-(5-fC/5-caC) or their BER-resistant 2′-fluorinated analogs, asymmetrically incorporated into CG-dinucleotide of the GC box cis -element (5′-TGGGCGGAGC) upstream from the RNA polymerase II core promoter. In the absence of BER, 5-caC caused a strong inhibition of the promoter activity, whereas 5-fC had almost no effect, similar to 5-methylcytosine or 5-hydroxymethylcytosine. BER of 5-caC caused a transient but significant promoter reactivation, succeeded by silencing during the following hours. Both responses strictly required thymine DNA glycosylase (TDG); however, the silencing phase additionally demanded a 5′-endonuclease (likely APE1) activity and was also induced by 5-fC or an apurinic/apyrimidinic site. We propose that 5-caC may act as a repressory mark to prevent premature activation of promoters undergoing the final stages of DNA demethylation, when the symmetric CpG methylation has already been lost. Remarkably, the downstream promoter activation or repression responses are regulated by two separate BER steps, where TDG and APE1 act as potential switches
- …