Lactic Acid Bacteria Enriched from Human Gastric Biopsies

The purpose of this paper was to check if viable bacteria, in particular lactic acid bacteria (LAB), could be enriched from biopsies obtained from healthy gastroscopy patients. Gastric biopsies were obtained from 13 gastroscopy patients and subjected to an anaerobic or microaerophilic enrichment procedure utilizing the Portable Microbe Enrichment Unit (PMEU). Profuse microbial growth was observed in most cases. Samples plated on MRS showed high numbers of LAB. The most common species characterized were Lactobacillus reuteri, Lact. salivarius, and Streptococcus salivarius. The results demonstrate a continuous presence of viable LAB in healthy stomach. The species are similar to those traditionally used in food applications. The gastric LAB strains could have a potential in developing probiotic foods aimed specially on the upper part of the gastrointestinal tract

Antimicrobial activity of Finnish organic honeys against human pathogenic bacteria

Proceeding volume: 13Peer reviewe

Antimicrobial Activity of Croton macrostachyus Stem Bark Extracts against Several Human Pathogenic Bacteria

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Antimicrobial activity of commercial organic honeys against clinical isolates of human pathogenic bacteria

Publisher Copyright: © 2022, The Author(s).Extracts of five organic and one conventional honey sources, available in Finnish markets, were tested for antimicrobial activity and inhibitory concentrations against Escherichia coli, Salmonella Typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus cereus, and Staphylococcus epidermidis, obtained from human specimens. Five (honeys A, B, D, E, F) of the six studied honeys were organic. All the studied honeys had inhibitory activity (zone of inhibition (ZI) > 9.4 ± 0.5 mm) compared to control artificial honey (ZI 13.3 mm against all the studied bacteria with minimal inhibitory concentration (MIC) values of 12.5–50%. Against E. coli, the organic honeys E and F had activity index (AI) of 0.64 and 0.73, respectively, compared to the antibiotic AI of 1.0. Against S. Typhi, the organic honeys D and F had AI of 0.59 and 0.64, respectively. Against P. aeruginosa, the organic honeys D, E, and F had the highest AI of 0.71–0.80, and against S. epidermidis the honeys B, D, E, and F possessed relatively high AI of 0.60, 0.67, 0.73, and 0.78, respectively. Against K. pneumoniae and B. cereus, the detected AI of the organic honeys B, D, E, and F varied between AI of 0.48 and 0.58. The organic honey A and conventionally produced honey C possessed only minor activity with MIC values of 80%. Here, we show that commercially available culinary organic honeys possess remarkable antimicrobial activity against several important human bacterial pathogens.Peer reviewe

Antimalarial Activity of Croton macrostachyus Stem Bark Extracts against Plasmodium berghei In Vivo

There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to test in vivo antiplasmodial activity of Croton macrostachyus H. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized with Plasmodium berghei (ANKA). Different doses (500, 250, and 100mg/kg) of C. macrostachyus ethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters' 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (PPeer reviewe

Examining Safety of Biocolourants from Fungal and Plant Sources-Examples from Cortinarius and Tapinella, Salix and Tanacetum spp. and Dyed Woollen Fabrics

Biocolourants have been investigated as alternatives to synthetic dyes. However, natural origin per se is not a label of harmlessness and research is needed to obtain safe dyes. We studied the cytotoxicity of the extracts from fungal (Cortinarius semisanguineus, Tapinella atrotomentosa) and plant (Tanacetum vulgare, Salix phylicifolia) sources and the woollen fabrics dyed with the extracts. Cytotoxicity in vitro using hepa-1 mouse hepatoma cells for 24 h and 72 h exposure was observed as the highest tolerated dose. All biocolourants produced intensive colour on fabrics with fastness properties from moderate to good. The Salix and Cortinarius samples did not show any cytotoxic effects, whereas the Tanacetum and Tapinella samples had slightly higher test values but were not interpreted as being significantly toxic. Higher than zero values of the undyed fabrics showed the importance of examining their toxicity as well. It was found that the cytotoxicity of the samples dyed with the biocolourants did not differ significantly from the undyed wool fabric. The concentrations of dyes used in the assays were very low, imitating the dose of the user. In addition to colouring properties, natural dyes may have pharmaceutical and antibacterial properties which would enhance the interest in using them in products for added value

Antibiotic resistance and probiotics properties of lactic acid bacteria isolated from Andean vegetable and animal products

In Andean regions of America, many traditional fermented foods and beverages are produced. Vegetable traditional products involve quinoa and amaranth whose widespread consumption as grains and fermented flours (sourdoughs) has grown recently due to their high nutritional value and lack of gluten. Fermented potatoes (Tocosh) have also been produced from ancient times in the Central Andean region of Peru, whereas llama meat fermented sausages are traditionally produced and consumed in Northwestern Argentina. At present, there is great concern that commensal bacterial populations from food and the gastrointestinal tract (GIT) of humans and animals, such as lactic acid bacteria (LAB) can serve as reservoir for antibiotic resistance genes, which can be horizontally transferred to other microorganisms. On these bases, the antibiotic resistance profiles (EFSA-2012) of eighty LAB strains isolated from Andean products were investigated. The analyzed exhibited a general sensitive profile against ampicilline (AMP), vancomycin (VAN), gentamycin (GEN), kanamycin (KAN), streptomycin (STR), erythromycin (ERY), clindamycin (CLI), tetracycline (TET) and chloramphenicol (CHL). Several strains were resistant mainly to the aminoglycosides (KAN, STR and GEN) while a multi-resistant pattern was found particularly among strains isolated from quinoa /amaranth grains and sourdoughs. However, sensitive LAB strains were observed among Lb. sakei, Lb. reuteri, Lb. plantarum, Lb. casei and Leuc. mesenteroides. On the other hand, to select LAB strains with probiotic potential the resistance to stress factors (lysozyme, bile salts and low pH) present in the GIT, surface properties (hydrophobicity, autoaggregation and adhesion to caco-2 cells) and production of antimicrobial compounds were evaluated. Results allowed selecting Lb. plantarum (2 strains), Lb. reuteri (1) and Lb. casei (1) as the best candidates to be used as probiotics.Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vera Pingitore, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Fontana, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Università Cattolica del Sacro Cuore; ItaliaFil: Cocconcelli, Pier. S.. Università Cattolica del Sacro Cuore; ItaliaFil: Jimenez, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Plumed Ferrer, Carmen. University Of Eastern Finland.; FinlandiaFil: von Wright, Atte. University Of Eastern Finland.; FinlandiaFil: Aznar, Rosa. Universidad de Valencia; España11th International Symposium on Lactic Acid Bacteria: Health, Sustainability, Diversity, and ApplicationEgmond aan ZeePaíses BajosRoyal Netherlands Society for MicrobiologyFederation of European Microbiological Societie

Питання тривалості консервативного лікування хворих на вперше виявлений туберкульоз легень

У роботі розглядається питання тривалості консервативного лікування хворих на вперше виявлений туберкульоз легень. При цитуванні документа, використовуйте посилання http://essuir.sumdu.edu.ua/handle/123456789/635

Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome

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