4,644 research outputs found
Muscle recruitment strategies can reduce joint loading during level walking
Joint inflammation, with consequent cartilage damage and pain, typically reduces functionality and affects activities of daily life in a variety of musculoskeletal diseases. Since mechanical loading is an important determinant of the disease process, a possible conservative treatment is the unloading of joints. In principle, a neuromuscular rehabilitation program aimed to promote alternative muscle recruitments could reduce the loads on the lower-limb joints during walking. The extent of joint load reduction one could expect from this approach remains unknown. Furthermore, assuming significant reductions of the load on the affected joint can be achieved, it is unclear whether, and to what extent, the other joints will be overloaded. Using subject-specific musculoskeletal models of four different participants, we computed the muscle recruitment strategies that minimised the hip, knee and ankle contact force, and predicted the contact forces such strategies induced at the other joints. Significant reductions of the peak force and impulse at the knee and hip were obtained, while only a minimal effect was found at the ankle joint. Adversely, the peak force and the impulse in non-targeted joints increased when aiming to minimize the load in an adjacent joint. These results confirm the potential of alternative muscle recruitment strategies to reduce the loading at the knee and the hip, but not at the ankle. Therefore, neuromuscular rehabilitation can be targeted to reduce the loading at affected joints but must be considered carefully in patients with multiple joints affected due to the potential adverse effects in non-targeted joints
The footprint of cometary dust analogues: II. Morphology as a tracer of tensile strength and application to dust collection by the Rosetta spacecraft
The structure of cometary dust is a tracer of growth processes in the
formation of planetesimals. Instrumentation on board the Rosetta mission to
comet 67P/Churyumov- Gerasimenko captured dust particles and analysed them in
situ. However, these deposits are a product of a collision within the
instrument. We conducted laboratory experiments with cometary dust analogues,
simulating the collection process by Rosetta instruments (specifically COSIMA,
MIDAS). In Paper I we reported that velocity is a key driver in determining the
appearance of deposits. Here in Paper II we use materials with different
monomer sizes, and study the effect of tensile strength on the appearance of
deposits. We find that mass transfer efficiency increases from 1 up to
10% with increasing monomer diameter from 0.3 m to 1.5 m (i.e.
tensile strength decreasing from 12 to 3 kPa), and velocities
increasing from 0.5 to 6 m/s. Also, the relative abundance of small fragments
after impact is higher for material with higher tensile strength. The
degeneracy between the effects of velocity and material strength may be lifted
by performing a closer study of the deposits. This experimental method makes it
possible to estimate the mass transfer efficiency in the COSIMA instrument.
Extrapolating these results implies that more than half of the dust collected
during the Rosetta mission has not been imaged. We analysed two COSIMA targets
containing deposits from single collisions. The collision that occurred closest
to perihelion passage led to more small fragments on the target.Comment: 13 pages, 11 figures, accepted for publication in MNRA
HST Snapshot Survey of Post-AGB Objects
The results from a Hubble Space Telescope (HST) snapshot survey of post-AGB
objects are shown. The aim of the survey is to complement existing HST images
of PPN and to connect various types of nebulosities with physical and chemical
properties of their central stars. Nebulosities are detected in 15 of 33
sources. Images and photometric and geometric measurements are presented. For
sources with nebulosities we see a morphological bifurcation into two groups,
DUPLEX and SOLE, as previous studies have found. We find further support to the
previous results suggesting that this dichotomy is caused by a difference in
optical thickness of the dust shell. The remaining 18 sources are classified as
stellar post-AGB objects, because our observations indicate a lack of
nebulosity. We show that some stellar sources may in fact be DUPLEX or SOLE
based on their infrared colors. The cause of the differences among the groups
are investigated. We discuss some evidence suggesting that high progenitor-mass
AGB stars tend to become DUPLEX post-AGB objects. Intermediate progenitor-mass
AGB stars tend to be SOLE post-AGB objects. Most of the stellar sources
probably have low mass progenitors and do not seem to develop nebulosities
during the post-AGB phase and therefore do not become planetary nebulae.Comment: 21 pages, 11 figure
Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis
The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity. We have constructed mutants of the adenosine triphosphate (ATP) binding cassette (ABC)-type MDR transporter LmrA of Lactococcus lactis that are defective in ATP hydrolysis. These mutants and wild-type LmrA exhibited an atypical behavior in the Hoechst 33342 transport assay. In membrane vesicles, Hoechst 33342 transport was shown to be independent of the ATPase activity of LmrA, and it was not inhibited by orthovanadate but sensitive to uncouplers that collapse the proton gradient and to N,N'-dicyclohexylcarbodiimide, an inhibitor of the F0F1-ATPase. In contrast, transport of Hoechst 33342 by the homologous, heterodimeric MDR transporter LmrCD showed a normal ATP dependence and was insensitive to uncouplers of the proton gradient. With intact cells, expression of LmrA resulted in an increased rate of Hoechst 33342 influx while LmrCD caused a decrease in the rate of Hoechst 33342 influx. Cellular toxicity assays using a triple knockout strain, i.e., L. lactis ÎlmrA ÎlmrCD, demonstrate that expression of LmrCD protects cells against the growth inhibitory effects of Hoechst 33342, while in the presence of LmrA, cells are more susceptible to Hoechst 33342. Our data demonstrate that the LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane.
Accumulation at South Pole: Comparison of two 900-year records
This is the published version, also available here: http://dx.doi.org/10.1029/1999JD900501.Two 900-year records of annual accumulation at South Pole are compared to evaluate the origin and significance of observed variations. Despite difficulties establishing absolute timescales, due to problems identifying annual layer markers, the two records can be correlated with confidence after moderate smoothing. This correlation shows that over the time period considered (1050â1956 A.D.) no climatically significant changes in accumulation occurred. Instead, fluctuations preserved in the two cores reflect spatial variations in snow accumulation, associated with nonuniform deposition induced by surface relief on the scale of several kilometers
The footprint of cometary dust analogs: I. Laboratory experiments of low-velocity impacts and comparison with Rosetta data
Cometary dust provides a unique window on dust growth mechanisms during the
onset of planet formation. Measurements by the Rosetta spacecraft show that the
dust in the coma of comet 67P/Churyumov-Gerasimenko has a granular structure at
size scales from sub-um up to several hundreds of um, indicating hierarchical
growth took place across these size scales. However, these dust particles may
have been modified during their collection by the spacecraft instruments. Here
we present the results of laboratory experiments that simulate the impact of
dust on the collection surfaces of COSIMA and MIDAS, instruments onboard the
Rosetta spacecraft. We map the size and structure of the footprints left by the
dust particles as a function of their initial size (up to several hundred um)
and velocity (up to 6 m/s). We find that in most collisions, only part of the
dust particle is left on the target; velocity is the main driver of the
appearance of these deposits. A boundary between sticking/bouncing and
fragmentation as an outcome of the particle-target collision is found at v ~ 2
m/s. For velocities below this value, particles either stick and leave a single
deposit on the target plate, or bounce, leaving a shallow footprint of
monomers. At velocities > 2 m/s and sizes > 80 um, particles fragment upon
collision, transferring up to 50 per cent of their mass in a rubble-pile-like
deposit on the target plate. The amount of mass transferred increases with the
impact velocity. The morphologies of the deposits are qualitatively similar to
those found by the COSIMA instrument.Comment: 14 pages, 12 figures, accepted for publication in MNRA
- âŠ