Connecting low- and high-mass star formation: the intermediate-mass protostar IRAS 05373+2349 VLA 2

Until recently, there have been few studies of the protostellar evolution of intermediate-mass (IM) stars, which may bridge the low- and high-mass regimes. This paper aims to investigate whether the properties of an IM protostar within the IRAS 05373+2349 embedded cluster are similar to that of low- and/or high-mass protostars. We carried out Very Large Array as well as Combined Array for Research in Millimeter Astronomy continuum and 12CO(J=1–0) observations, which uncover seven radio continuum sources (VLA 1–7). The spectral index of VLA 2, associated with the IM protostar is consistent with an ionized stellar wind or jet. The source VLA 3 is coincident with previously observed H2 emission line objects aligned in the north–south direction (P.A. −20 to −12◦), which may be either an ionized jet emanating from VLA 2 or (shock-)ionized cavity walls in the large-scale outflow from VLA 2. The position angle between VLA 2 and 3 is slightly misaligned with the large-scale outflow we map at ∼5-arcsec resolution in 12CO (P.A. ∼30◦), which in the case of a jet suggests precession. The emission from the mm core associated with VLA 2 is also detected; we estimate its mass to be 12–23 M , depending on the contribution from ionized gas. Furthermore, the large-scale outflow has properties intermediate between outflows from low- and high-mass young stars. Therefore, we conclude that the IM protostar within IRAS 05373+2349 is phenomenologically as well as quantitatively intermediate between the low- and high-mass domains

Digit-only sauropod pes trackways from China - evidence of swimming or a preservational phenomenon?

For more than 70 years unusual sauropod trackways have played a pivotal role in debates about the swimming ability of sauropods. Most claims that sauropods could swim have been based on manus-only or manus-dominated trackways. However none of these incomplete trackways has been entirely convincing, and most have proved to be taphonomic artifacts, either undertracks or the result of differential depth of penetration of manus and pes tracks, but otherwise showed the typical pattern of normal walking trackways. Here we report an assemblage of unusual sauropod tracks from the Lower Cretaceous Hekou Group of Gansu Province, northern China, characterized by the preservation of only the pes claw traces, that we interpret as having been left by walking, not buoyant or swimming, individuals. They are interpreted as the result of animals moving on a soft mud-silt substrate, projecting their claws deeply to register their traces on an underlying sand layer where they gained more grip during progression. Other sauropod walking trackways on the same surface with both pes and manus traces preserved, were probably left earlier on relatively firm substrates that predated the deposition of soft mud and silt . Presently, there is no convincing evidence of swimming sauropods from their trackways, which is not to say that sauropods did not swim at all

Spiral arms and instability within the AFGL 4176 mm1 disc

We present high-resolution (30 mas or 130 au at 4.2 kpc) Atacama Large Millimeter/submillimeter Array observations at 1.2 mm of the disc around the forming O-type star AFGL 4176 mm1. The disc (AFGL 4176 mm1-main) has a radius of ∼1000 au and contains significant structure, most notably a spiral arm on its redshifted side. We fitted the observed spiral with logarithmic and Archimedean spiral models. We find that both models can describe its structure, but the Archimedean spiral with a varying pitch angle fits its morphology marginally better. As well as signatures of rotation across the disc, we observe gas arcs in CH3CN that connect to other millimetre continuum sources in the field, supporting the picture of interactions within a small cluster around AFGL 4176 mm1-main. Using local thermodynamic equilibrium modelling of the CH3CN K-ladder, we determine the temperature and velocity field across the disc, and thus produce a map of the Toomre stability parameter. Our results indicate that the outer disc is gravitationally unstable and has already fragmented or is likely to fragment in the future, possibly producing further companions. These observations provide evidence that disc fragmentation is one possible pathway towards explaining the high fraction of multiple systems around high-mass stars

A search for non-thermal radio emission from jets of massive young stellar objects

Massive young stellar objects (MYSOs) have recently been shown to drive jets whose particles can interact with either the magnetic fields of the jet or ambient medium to emit non-thermal radiation. We report a search for non-thermal radio emission from a sample of 15 MYSOs to establish the prevalence of the emission in the objects. We used their spectra across the L, C, and Q bands along with spectral index maps to characterize their emission. We find that about 50 per cent of the sources show evidence for non-thermal emission with 40 per cent showing clear non-thermal lobes, especially sources of higher bolometric luminosity. The common or IRAS names of the sources that manifest non-thermal lobes are V645Cyg, IRAS 22134+5834, NGC 7538 IRS 9, IRAS 23262 + 640, AFGL 402d, and AFGL 490. All the central cores of the sources are thermal with corresponding mass-loss rates that lie in the range of ∼3 × 10−7 to 7×10−6M⊙yr−1⁠. Given the presence of non-thermal lobes in some of the sources and the evidence of non-thermal emission from some spectral index maps, it seems that magnetic fields play a significant role in the jets of massive protostars. Also noted is that some of the sources show evidence of binarity and variability

Modeling inflammation and oxidative stress in gastrointestinal disease development using novel organotypic culture systems

Gastroesophageal reflux disease (GERD), Barrett's esophagus (BE), graft-versus-host disease (GVHD), and inflammatory bowel diseases such as ulcerative colitis and Crohn's disease are common human gastrointestinal diseases that share inflammation as a key driver for their development. A general outcome resulting from these chronic inflammatory conditions is increased oxidative stress. Oxidative stress is caused by the generation of reactive oxygen and nitrogen species that are part of the normal inflammatory response, but are also capable of damaging cellular DNA, protein, and organelles. Damage to DNA can include DNA strand breaks, point mutations due to DNA adducts, as well as alterations in methylation patterns leading to activation of oncogenes or inactivation of tumor suppressors. There are a number of significant long-term consequences associated with chronic oxidative stress, most notably cancer. Infiltrating immune cells and stromal components of tissue including fibroblasts contribute to dynamic changes occurring in tissue related to disease development. Immune cells can potentiate oxidative stress, and fibroblasts have the capacity to contribute to advanced growth and proliferation of the epithelium and any resultant cancers. Disease models for GERD, BE, GVHD, and ulcerative colitis based on three-dimensional human cell and tissue culture systems that recapitulate in vivo growth and differentiation in inflammatory-associated microphysiological environments would enhance our understanding of disease progression and improve our ability to test for disease-prevention strategies. The development of physiologically relevant, human cell-based culture systems is therefore a major focus of our research. These novel models will be of enormous value, allowing us to test hypotheses and advance our understanding of these disorders, and will have a translational impact allowing us to more rapidly develop therapeutic and chemopreventive agents. In summary, this work to develop advanced human cell-based models of inflammatory conditions will greatly improve our ability to study, prevent, and treat GERD, BE, GVHD, and inflammatory bowel disease. The work will also foster the development of novel therapeutic and preventive strategies that will improve patient care for these important clinical conditions. © 2013 BioMed Central Ltd

A Detailed View of the Circumstellar Environment and Disk of the Forming O-star AFGL 4176

We present a detailed analysis of the disk and circumstellar environment of the forming O-type star AFGL 4176 mm1, placing results from the Atacama Large Millimeter/submillimeter Array (ALMA) into context with multiwavelength data. With ALMA, we detect seventeen 1.2 mm continuum sources within 5'' (21,000 au) of AFGL 4176 mm1. We find that mm1 has a spectral index of 3.4 ± 0.2 across the ALMA band, with >87% of its 1.2 mm continuum emission from dust. The source mm2, projected 4200 au from mm1, may be a companion or a blueshifted knot in a jet. We also explore the morphological differences between the molecular lines detected with ALMA, finding 203 lines from 25 molecules, which we categorize into several morphological types. Our results show that AFGL 4176 mm1 provides an example of a forming O-star with a large and chemically complex disk, which is mainly traced by nitrogen-bearing molecules. Lines that show strong emission on the blueshifted side of the disk are predominantly oxygen-bearing, which we suggest are tracing a disk accretion shock. The molecules C34S, H2CS, and CH3CCN trace a slow wide-angle wind or dense structures in the outflow cavity walls. With the Australia Telescope Compact Array (ATCA), we detect a compact continuum source (96% is from ionized gas. The ATCA NH3(1, 1) and (2, 2) emission traces a large-scale (r ~ 0.5 pc) rotating toroid with the disk source mm1 in the blueshifted part of this structure offset to the northwest

Inverse magnetic catalysis in dense holographic matter

We study the chiral phase transition in a magnetic field at finite temperature and chemical potential within the Sakai-Sugimoto model, a holographic top-down approach to (large-N_c) QCD. We consider the limit of a small separation of the flavor D8-branes, which corresponds to a dual field theory comparable to a Nambu-Jona Lasinio (NJL) model. Mapping out the surface of the chiral phase transition in the parameter space of magnetic field strength, quark chemical potential, and temperature, we find that for small temperatures the addition of a magnetic field decreases the critical chemical potential for chiral symmetry restoration - in contrast to the case of vanishing chemical potential where, in accordance with the familiar phenomenon of magnetic catalysis, the magnetic field favors the chirally broken phase. This "inverse magnetic catalysis" (IMC) appears to be associated with a previously found magnetic phase transition within the chirally symmetric phase that shows an intriguing similarity to a transition into the lowest Landau level. We estimate IMC to persist up to 10^{19} G at low temperatures.Comment: 42 pages, 11 figures, v3: extended discussion; new appendix D; references added; version to appear in JHE

A transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognita

© The Author(s). 2018Background: Southern root-knot nematode Meloidogyne incognita (Kofoid and White, 1919), Chitwood, 1949 is a key pest of agricultural crops. Pasteuria penetrans is a hyperparasitic bacterium capable of suppressing the nematode reproduction, and represents a typical coevolved pathogen-hyperparasite system. Attachment of Pasteuria endospores to the cuticle of second-stage nematode juveniles is the first and pivotal step in the bacterial infection. RNA-Seq was used to understand the early transcriptional response of the root-knot nematode at 8 h post Pasteuria endospore attachment. Results: A total of 52,485 transcripts were assembled from the high quality (HQ) reads, out of which 582 transcripts were found differentially expressed in the Pasteuria endospore encumbered J2 s, of which 229 were up-regulated and 353 were down-regulated. Pasteuria infection caused a suppression of the protein synthesis machinery of the nematode. Several of the differentially expressed transcripts were putatively involved in nematode innate immunity, signaling, stress responses, endospore attachment process and post-attachment behavioral modification of the juveniles. The expression profiles of fifteen selected transcripts were validated to be true by the qRT PCR. RNAi based silencing of transcripts coding for fructose bisphosphate aldolase and glucosyl transferase caused a reduction in endospore attachment as compared to the controls, whereas, silencing of aspartic protease and ubiquitin coding transcripts resulted in higher incidence of endospore attachment on the nematode cuticle. Conclusions: Here we provide evidence of an early transcriptional response by the nematode upon infection by Pasteuria prior to root invasion. We found that adhesion of Pasteuria endospores to the cuticle induced a down-regulated protein response in the nematode. In addition, we show that fructose bisphosphate aldolase, glucosyl transferase, aspartic protease and ubiquitin coding transcripts are involved in modulating the endospore attachment on the nematode cuticle. Our results add new and significant information to the existing knowledge on early molecular interaction between M. incognita and P. penetrans.Peer reviewedFinal Published versio

Optimality of mutation and selection in germinal centers

The population dynamics theory of B cells in a typical germinal center could play an important role in revealing how affinity maturation is achieved. However, the existing models encountered some conflicts with experiments. To resolve these conflicts, we present a coarse-grained model to calculate the B cell population development in affinity maturation, which allows a comprehensive analysis of its parameter space to look for optimal values of mutation rate, selection strength, and initial antibody-antigen binding level that maximize the affinity improvement. With these optimized parameters, the model is compatible with the experimental observations such as the ~100-fold affinity improvements, the number of mutations, the hypermutation rate, and the "all or none" phenomenon. Moreover, we study the reasons behind the optimal parameters. The optimal mutation rate, in agreement with the hypermutation rate in vivo, results from a tradeoff between accumulating enough beneficial mutations and avoiding too many deleterious or lethal mutations. The optimal selection strength evolves as a balance between the need for affinity improvement and the requirement to pass the population bottleneck. These findings point to the conclusion that germinal centers have been optimized by evolution to generate strong affinity antibodies effectively and rapidly. In addition, we study the enhancement of affinity improvement due to B cell migration between germinal centers. These results could enhance our understandings to the functions of germinal centers.Comment: 5 figures in main text, and 4 figures in Supplementary Informatio