THE CURATIVE EFFECT OF METHYLSULFONYLMETHANE ON AN EXPERIMENTAL MODEL OF ULCERATIVE COLITIS IN RATS

Objective: Ulcerative colitis is a chronic inflammatory bowel disease of unknown etiology that affects the mucosa and submucosa of the colon and rectum. It is associated with oxidative stress, neutrophil infiltration and production of inflammatory mediators. In the present study, the anti-inflammatory and antioxidant effects of Methylsulfonylmethane (MSM) in treatment the acetic acid (A) induced model for UC in rats were examined. Methods: Ulcerative colitis was induced by intra colonic administration of 2 ml of 4% acetic acid in adult male Wistar rats which were divided into 3 groups: normal control (N), acetic acid (A) control, and MSM (1000 mg/kg). Rats received treatment for six consecutive days. On day 7, the rats were sacrificed, the colon was removed, the body weight, colon weight/length ratio, tissue glutathione (GSH) concentration, and macroscopic evaluations, were performed. Results: The colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased, but it didn’t reduce significantly in the MSM treated groups compared to group A. Contrariwise the parameters of group A. The levels of body weight were increased, and colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased significantly in the MSM treated group, compared to the acetic acid-treated group. Conclusions: Methylsulfonylmethane is an effective, safety natural product with little side effects, has a good curative effect in a dose (1000 mg/kg) on experimental Ulcerative colitis induced by acetic acid

The curative effects of methylsulfonylmethane against glycerol-induced acute renal failure in rats

The present study was performed to explore the curative effect of Methylsulfonylmethane (MSM) in an experimental model of myoglobinuric acute renal failure (ARF). In this experimental model, Rats were injected with 50% glycerol (10 mL/kg, im) followed by an hour later and daily in the next six days by MSM (400 mg/kg) or saline. Kidney’s function (urea and creatinine), and reduced glutathione were analyzed. A renal failure produced by glycerol injection, with a significant increase of blood urea and serum creatinine was observed. Rats that received MSM in addition to glycerol had significantly lower blood urea and serum creatinine levels compared to those receiving glycerol alone. However, glutathione has markedly increased after MSM treatment. The effect is probably due to the antioxidant activity of MSM. This may provide therapeutic opportunities for treating humans, myoglobinuric ARF

SCREENING THE ANTIMICROBIAL ACTIVITY OF SOME MEDICINAL PLANTS AGAINST MULTIDRUG RESISTANCE ESCHERICHIA COLI TYPE (1)

The increasing number of Escherichia coli causing mastitis and of bacteria resistant to conventional antibiotics has become aserious problem in recent years. So the search for new antibiotics and alternative products to solve this problem is the question ofthe age. This research aims to investigate the effectiveness of the extracts prepared from different parts of the following plants:Olea europea Linn (Oleaceae) ، Myrtus communis Linn (Liliaceae)، Majorana syriacus Linn (Laminaceae)، Zingiber officinaleLinn (Zingiberaceae)، Achillea falcata Linn (Asteraceae) against resistant Escherichia coli Type (1). Investigation began forE.coli bacteria in 667 milk samples. The bacteria were identified culturally, morphologically and biochemically. Antibioticsusceptibility testing against E.coli by Kirby-Bauer disk diffusion method were conducted. Then using the blood agar,MacConkey agar, salmonella - shigella agar, and biochemical testing method [API 20 E testing Enterobacteriaceae] were made totype E.coli. Plants were extracted with water, absolute alcohol, then ether using a soxhlet apparatus and rotary vacuumevaporator. Then extracts susceptibility testing against antibiotic resistant E.coli Type (1) were studied. E. coli was defined asoxidase negative, indole positive, catalase positive. The studied antibiotics did not show any antibacterial effect against E.coli .By the results of the biochemical analysis (API20e) on resistant E.coli , E.coli type (1) was 33.35% of the total number ofsamples. The anti-bacterial effectiveness against E.coli type (1) of ethanol extracts prepared from different parts of the studiedplants were variant, whereas the Myrtus communis extract effectively has the most powerful antibacterial effect for these bacteria,while the Zingiber officinale extract has the lowest influence

ANTIBACTERIAL EFFICACY OF VARIETY PLANTS AGAINST THE RESISTANT STREPTOCOCCUS WHICH CAUSE CLINICAL MASTITIS IN COWS

Streptococcus is considered to be one of the most dangerous causes of Clinical mastitis in cows. The aim of this study was to investigate the effectiveness of extracts prepared from different parts of the flowing plants: Oleaeuropea Linn(Oleaceae), Myrtuscommunis Linn (Liliaceae) ,thymus vulgaris Linn (Laminaceae), Rosemery Linn(Laminaceae), Ficuscarica Linn (Moraceae), and Achilleafalcata Linn(Asteraceae) against resistant Streptococcus in 1371 Samples of milk. This work was achieved in four stages: First of all, the presence of Streptococcus in 1371 Samples of milk, by using blood agar, Bile Esculin agar, and some bio-chemical tests were investigated .Secondly, the antibacterial activity of many antibiotics on these bacteria by using disc diffusion method were determined.Thirdly, the plants were extracted with water, absolute alcohol, and petroleum Ether by using soxhlet apparatus and rotary vacuum evaporator. Fourthly, the antibacterial activity of the extractions on resistant Streptococcus was determined by using disc diffusion method. This study has shown the presence of different antibacterial effectiveness of the extracts prepared from different parts of those plants. The extract of thymus vulgaris is more effective when compared to the extract of Oleaeur opaea against resistant Streptococcus.

Histopathological Changes of the Effect of Ketotifen in a Rat Model of Nephropathy

Acute kidney injury (AKI) remains a common clinical problem with serious consequences and unsatisfactory therapeutic options. Mast cells are distributed in the kidneys, have a role in the inflammation. Thus, a model of Acute kidney injury of rhabdomyolysis induced by glycerol was done in order to investigate the possible healing effect of Ketotifen, a selective stabilizer of mast cells and a histamine H1 receptor antagonist, in rats. Methods: Wister rats (250-350 g) were used. Renal failure was produced by rats deprived of water for 24 hours followed by i.m. injection with 50% (vol/vol) glycerol (10 ml/kg of body weight). After 30 min, Ketotifen was used at a dose of 2 mg/kg. Rats received treatment for 5 consecutive days. On the 6th day, the rats were sacrificed; blood was obtained for blood urea and creatinine assays. The kidney tissue was used for the determination the histological injury. Results: The levels of urea and creatinine were decreased significantly

The curative effects of methylsulfonylmethane against glycerol-induced acute renal failure in rats

Abstract The present study was performed to explore the curative effect of Methylsulfonylmethane (MSM) in an experimental model of myoglobinuric acute renal failure (ARF). In this experimental model, Rats were injected with 50% glycerol (10 mL/kg, im) followed by an hour later and daily in the next six days by MSM (400 mg/kg) or saline. Kidney’s function (urea and creatinine), and reduced glutathione were analyzed. A renal failure produced by glycerol injection, with a significant increase of blood urea and serum creatinine was observed. Rats that received MSM in addition to glycerol had significantly lower blood urea and serum creatinine levels compared to those receiving glycerol alone. However, glutathione has markedly increased after MSM treatment. The effect is probably due to the antioxidant activity of MSM. This may provide therapeutic opportunities for treating humans, myoglobinuric ARF

Methylsulfonylmethane (MSM) as a New Target for the Treatment of Glycerol-Induced Nephropathy

Objective: Methylsulfonylmethane (MSM) is a naturally occurring nutrient composed of sulfur, oxygen and methyl groups. MSM may have anti-inflammatory and free radical scavenging activity. The present work was done to investigate the possible cure effect of methylsulfonylmethane on glycerol-induced acute renal failure (ARF) in rats.Methodes: After water deprivation, a kidney injury was induced in rats by intramuscular administration of glycerol 10 mL/kg (50% vol./vol. in saline). Several parameters including macroscopic score, histopathological and biochemical were determined to assess the degree of treatment.Results: Results showed that MSM decreased macroscopic and microscopic kidney’s injury scores caused by glycerol. MSM also significantly reduced urea and creatinine levels compared to glycerol-induced ARF group.Conclusion: MSM as a natural product has a curing effect against glycerol-induced myoglobinuria

The curative effects of methylsulfonylmethane against glycerol-induced acute renal failure in rats

<div><p>Abstract The present study was performed to explore the curative effect of Methylsulfonylmethane (MSM) in an experimental model of myoglobinuric acute renal failure (ARF). In this experimental model, Rats were injected with 50% glycerol (10 mL/kg, im) followed by an hour later and daily in the next six days by MSM (400 mg/kg) or saline. Kidney’s function (urea and creatinine), and reduced glutathione were analyzed. A renal failure produced by glycerol injection, with a significant increase of blood urea and serum creatinine was observed. Rats that received MSM in addition to glycerol had significantly lower blood urea and serum creatinine levels compared to those receiving glycerol alone. However, glutathione has markedly increased after MSM treatment. The effect is probably due to the antioxidant activity of MSM. This may provide therapeutic opportunities for treating humans, myoglobinuric ARF.</p></div

Evaluation of Protective Effect of Methyl Sulfonyl Methane on Colon Ulcer Induced by Alendronate

Background: Bisphosphonates represent a new class of drugs that have shown very promising therapeutic efficacy in the treatment of a number of diseases associated with abnormally accelerated bone resorption including osteoporosis, Paget’s disease, and hypercalcemia of malignancy. As an increasing number of these drugs become available for clinical use in the treatment of the millions of individuals with these skeletal diseases, evidence is becoming available that their chronic usage may be associated with a number of gastrointestinal side effects, such as diarrhea, abdominal pain and inflammation, and erosions and ulceration of the upper gastrointestinal tract. Methyl sulfonyl methane (MSM) which is used for osteoarthritis was used to prevent bisphosphonates ulceration. Our objective was to study the preventive effect of MSM against colon ulcer induced by Alendronate (ALN) in rats. The experiments had been done on 8 white wistar rats for each group. The gastric ulcer has been induced by administration of Alendronate (20mg/kg/day) by gavage for 4 days. MSM (400mg/kg/day) has been given for the protective group for 4 days before administration of Alendronate. The ulcers in rats' colon were examined histologically and microscopically. The results showed that administration of MSM before Alendronate inducing ulcer led to a reduction in ulceration and showed significant difference comparing with morbidity group. Conclusion: MSM (400 mg/kg/day) has protective effect of colon ulcer induced by alendronate

Evaluation of Protective Effect of Methyl Sulfonyl Methane on Colon Ulcer Induced by Alendronate

Background: Bisphosphonates represent a new class of drugs that have shown very promising therapeutic efficacy in the treatment of a number of diseases associated with abnormally accelerated bone resorption including osteoporosis, Paget’s disease, and hypercalcemia of malignancy. As an increasing number of these drugs become available for clinical use in the treatment of the millions of individuals with these skeletal diseases, evidence is becoming available that their chronic usage may be associated with a number of gastrointestinal side effects, such as diarrhea, abdominal pain and inflammation, and erosions and ulceration of the upper gastrointestinal tract. Methyl sulfonyl methane (MSM) which is used for osteoarthritis was used to prevent bisphosphonates ulceration. Our objective was to study the preventive effect of MSM against colon ulcer induced by Alendronate (ALN) in rats. The experiments had been done on 8 white wistar rats for each group. The gastric ulcer has been induced by administration of Alendronate (20mg/kg/day) by gavage for 4 days. MSM (400mg/kg/day) has been given for the protective group for 4 days before administration of Alendronate. The ulcers in rats' colon were examined histologically and microscopically. The results showed that administration of MSM before Alendronate inducing ulcer led to a reduction in ulceration and showed significant difference comparing with morbidity group. Conclusion: MSM (400 mg/kg/day) has protective effect of colon ulcer induced by alendronate