Through the dark continent: African trypanosome development in the tsetse fly

African trypanosomes are unicellular flagellated parasites causing trypanosomiases in Africa, a group of severe diseases also known as sleeping sickness in human and nagana in cattle. These parasites are almost exclusively transmitted by the bite of the tsetse fly. In this review, we describe and compare the three developmental programs of the main trypanosome species impacting human and animal health, with focus on the most recent observations. From here, some reflections are made on research issues concerning trypanosome developmental biology in the tsetse fly that are to be addressed in the future

Total cost of ownership as a tool for inter-firm cost management: a case in the Belgian utilities industry.

In today's environment, communicating what your products and services are worth to customers' business has never been more important. Customers increasingly look at purchasing as a way to increase profits and to reduce costs. To persuade customers to focus on the total costs rather than simply on acquisition price, a supplier must have an accurate understanding of what his customers value. In this case study, we demonstrate how a utility supplier performed a Total Cost of Ownership analysis for one of his customers. The case study offers insights in how an inter-firm Total Cost of Ownership analysis can be beneficial to the buyer as well as to the supplier by optimising and better coordinating the performance of operations across the value chain and by facilitating further initiatives to intensify the buyer-supplier relationship.Product; Purchasing; Costs; Cost; Value; Case studies; Studies; Utilities; Performance; Management; Industry;

The effect of cost information on buyer-supplier negotiations in different power settings.

We investigate the influence of cost information on buyer-supplier negotiations in different power settings. Based on exchange theory, we expect that buyers with detailed cost information and less power than their opponent may try to (re)gain control over their own outcomes by sharing information. The results of our experiment indicate that the performance disadvantage of less powerful buyers is less pronounced when the buyer has detailed cost information and that this result can be explained by the buyer's negotiation behavior.Behavior; Control; Cost; Exchange; Information; Negotiations;

Franchising: A literature review on management and control issues.

Franchising; Literature review; Management control;

The effect of cost information on buyer-supplier negotiations in different power settings.

buyer-supplier negotiations; cost information;

Description of a nanobody-based competitive immunoassay to detect tsetse fly exposure

Background : Tsetse flies are the main vectors of human and animal African trypanosomes. The Tsal proteins in tsetse fly saliva were previously identified as suitable biomarkers of bite exposure. A new competitive assay was conceived based on nanobody (Nb) technology to ameliorate the detection of anti-Tsal antibodies in mammalian hosts. Methodology/Principal Findings : A camelid-derived Nb library was generated against the Glossina morsitans morsitans sialome and exploited to select Tsal specific Nbs. One of the three identified Nb families (family III, TsalNb-05 and TsalNb-11) was found suitable for anti-Tsal antibody detection in a competitive ELISA format. The competitive ELISA was able to detect exposure to a broad range of tsetse species (G. morsitans morsitans, G. pallidipes, G. palpalis gambiensis and G. fuscipes) and did not cross-react with the other hematophagous insects (Stomoxys calcitrans and Tabanus yao). Using a collection of plasmas from tsetse-exposed pigs, the new test characteristics were compared with those of the previously described G. m. moristans and rTsal1 indirect ELISAs, revealing equally good specificities (> 95%) and positive predictive values (> 98%) but higher negative predictive values and hence increased sensitivity (> 95%) and accuracy (> 95%). Conclusion/Significance : We have developed a highly accurate Nb-based competitive immunoassay to detect specific anti-Tsal antibodies induced by various tsetse fly species in a range of hosts. We propose that this competitive assay provides a simple serological indicator of tsetse fly presence without the requirement of test adaptation to the vertebrate host species. In addition, the use of monoclonal Nbs for antibody detection is innovative and could be applied to other tsetse fly salivary biomarkers in order to achieve a multi-target immunoprofiling of hosts. In addition, this approach could be broadened to other pathogenic organisms for which accurate serological diagnosis remains a bottleneck

Total cost of ownership: Informatie voor het optimaliseren van de supply chain.

Total cost of ownership; Cost; Ownership; Supply chain;

Nanostructured polymer coatings for controlling the wettability of fibrous surfaces

In this research we present a new approach for modifying and functionalizing the surface properties of fibrous substrates, especially cellulose-based products, by the deposition of organic nanoparticle coatings. The partial imidisation of high-molecular weight poly(styrene-maleic anhydride) in presence of ammonium hydroxide results in a stable water-borne dispersion of nanoparticles with sizes of 50 to 100 nm. The hydrophobic properties of coated paper surfaces are governed by a unique combination of chemical (degree of imidisation) and topographic (porosity and roughness) effects: it has been found that the contact angle increases at a higher degree of imidisation and higher surface roughness. As such, maximum advancing contact angles in the range of 140 to 150° and receding contact angles of 50° can be obtained on paper surfaces