5,282 research outputs found
Complications and outcome of cats with congenital extrahepatic portosystemic shunts treated with thin film: Thirty-four cases (2008-2017)
Background:
Congenital extrahepatic portosystemic shunts (CEHPSS) are rare in cats. Outcome after attenuation of CEHPSS with thin film has been described in a small number of cases.
Objectives:
To describe the clinical presentation, postoperative complications, and outcome of cats treated with thin film to attenuate CEHPSS.
Animals:
Thirtyâfour cats with CEHPSS were identified from the database of 3 institutions over 9âyears.
Methods:
Retrospective study. Medical records were reviewed to identify cats with a diagnosis of a CEHPSS that underwent surgical attenuation. Congenital extrahepatic portosystemic shunts were suspected from clinical signs, clinicopathologic findings, and diagnostic imaging, and confirmed at exploratory laparotomy. Cats treated with thin film band attenuation were included. Postoperative complications and followâup were recorded.
Results:
Complications were recorded in 11 of 34 cats. Deaths related to CEHPSS occurred in 6 of 34; 4 cats did not survive to discharge. Persistent seizures were the cause of death in 4 cats. Seizures were recorded in 8 of 34 cats after surgery; all these cats received preoperative antiepileptic drugs. Serum bile acid concentrations normalized in 25 of 28 of the cats for which data was available. Three cats had persistently increased serum bile acid concentrations and underwent a second exploratory laparotomy. One had a patent shunt, the other 2 had multiple acquired portosystemic shunts. Median followâup was 8âmonths (0.5â84âmonths).
Conclusions and Clinical Importance:
Congenital extrahepatic portosystemic shunts attenuation using thin film in cats carries a good shortâ and midâterm prognosis if they survive the postoperative period. Seizures were the most common cause of death
Long-term clinical outcomes following surgery for spontaneous pneumothorax caused by pulmonary blebs and bullae in dogs â a multicentre (AVSTS Research Cooperative) retrospective study
Performance of Glass Resistive Plate Chambers for a high granularity semi-digital calorimeter
A new design of highly granular hadronic calorimeter using Glass Resistive
Plate Chambers (GRPCs) with embedded electronics has been proposed for the
future International Linear Collider (ILC) experiments. It features a 2-bit
threshold semi-digital read-out. Several GRPC prototypes with their electronics
have been successfully built and tested in pion beams. The design of these
detectors is presented along with the test results on efficiency, pad
multiplicity, stability and reproducibility.Comment: 16 pages, 15 figure
Construction and commissioning of a technological prototype of a high-granularity semi-digital hadronic calorimeter
A large prototype of 1.3m3 was designed and built as a demonstrator of the
semi-digital hadronic calorimeter (SDHCAL) concept proposed for the future ILC
experiments. The prototype is a sampling hadronic calorimeter of 48 units. Each
unit is built of an active layer made of 1m2 Glass Resistive Plate
Chamber(GRPC) detector placed inside a cassette whose walls are made of
stainless steel. The cassette contains also the electronics used to read out
the GRPC detector. The lateral granularity of the active layer is provided by
the electronics pick-up pads of 1cm2 each. The cassettes are inserted into a
self-supporting mechanical structure built also of stainless steel plates
which, with the cassettes walls, play the role of the absorber. The prototype
was designed to be very compact and important efforts were made to minimize the
number of services cables to optimize the efficiency of the Particle Flow
Algorithm techniques to be used in the future ILC experiments. The different
components of the SDHCAL prototype were studied individually and strict
criteria were applied for the final selection of these components. Basic
calibration procedures were performed after the prototype assembling. The
prototype is the first of a series of new-generation detectors equipped with a
power-pulsing mode intended to reduce the power consumption of this highly
granular detector. A dedicated acquisition system was developed to deal with
the output of more than 440000 electronics channels in both trigger and
triggerless modes. After its completion in 2011, the prototype was commissioned
using cosmic rays and particles beams at CERN.Comment: 49 pages, 41 figure
The synovial and blood monocyte DNA methylomes mirror prognosis, evolution and treatment in early arthritis
Identifying predictive biomarkers at early stages of early inflammatory arthritis is crucial for starting appropriate therapies to avoid poor outcomes. Monocytes and macrophages, largely associated with arthritis, are contributors and sensors of inflammation through epigenetic modifications. In this study, we investigated associations between clinical features and DNA methylation in blood and synovial fluid (SF) monocytes in a prospective cohort of early inflammatory arthritis patients. Undifferentiated arthritis (UA) blood monocyte DNA methylation profiles exhibited significant alterations in comparison with those from healthy donors. We identified additional differences both in blood and SF monocytes after comparing UA patients grouped by their future outcomes, good versus poor. Patient profiles in subsequent visits revealed a reversion towards a healthy level in both groups, those requiring disease-modifying antirheumatic drugs (DMARDs) and those that remitted spontaneously. Changes in disease activity between visits also impacted DNA methylation, partially concomitant in the SF of UA and in blood monocytes of rheumatoid arthritis patients. Epigenetic similarities between arthritis types allow a common prediction of disease activity. Our results constitute a resource of DNA methylation-based biomarkers of poor prognosis, disease activity and treatment efficacy in early untreated UA patients for the personalized clinical management of early inflammatory arthritis patients
Is the caudal auricular axial pattern flap robust? A multi-centre cohort study of 16 dogs and 12 cats (2005 to 2016)
Estudio del perfil genético en pacientes con Amiloidosis de cadenas ligeras
Oral Presentation [CO-130]
IntroducciĂłn: Los estudios de secuenciaciĂłn masiva (NGS) han permitido profundizar en el conocimiento de las gammapatĂas monoclonales tales como el mieloma mĂșltiple (MM) y la macroglobulinemia de Waldesntrömâs (WM). Desafortunadamente, la baja incidencia de la amiloidosis de cadenas ligeras (AL) y la baja carga tumoral que presenta, a menudo enmascarada por un fondo policlonal de cĂ©lulas plasmĂĄticas (PC), explica la poca informaciĂłn que hay sobre la biologĂa de la cĂ©lula tumoral. Por ello, se desconoce si la AL presenta alguna mutaciĂłn comĂșn como ocurre en la WM, si existen mutaciones recurrentes, y si estas podrĂan coincidir con las observadas en MM. Por lo tanto, el objetivo de este trabajo es realizar una secuenciaciĂłn de exoma (WES) en una serie de pacientes con AL y comparar su perfil mutacional con el de MM.
Métodos: En este estudio se incluyeron 28 pacientes con AL. Se realizó un WES, incluyendo las regiones reguladoras UTR (SureSelect Human All Exon V6 + UTRs (Agilent)) en 56 muestras pareadas sorteadas de células plasmåticas patológicas y sangre periférica como muestra control. Cada muestra tumoral fue capturada por triplicado y secuenciada en la plataforma NextSeq 500 (Illumina). Para el anålisis de variantes somåticas se utilizaron los programas Strelka y ANNOVAR. . Las firmas mutacionales se analizaron con el software DeconstructSigs. Para comparar el perfil mutacional de AL con MM se utilizó la base de datos MMRF CoMMpass con 895 pacientes. Ademås, se han determinado los reordenamientos de los genes de las inmunoglobulinas (Igs) mediante NGS.
Resultados: La cobertura media de secuenciaciĂłn para las muestras de control y tumor fue de 64x y 186x, respectivamente. Se detectaron un total de 1983 SNV y 133 INDEL con una media de 71 (20-281) SNV y 5 (0-25) INDEL por paciente. Al comparar con MM (media 66 SNV y 2.5 INDEL) se observĂł una carga mutacional similar. Los Ășnicos genes mutados tanto en AL como en MM fueron MUC16 (recurrencia 17% y 8%, respectivamente) e IGLL5 (recurrencia 17%, en ambas), siendo ademĂĄs los genes mĂĄs frecuentemente mutados en AL Las firmas mutacionales mĂĄs frecuentes que se identificaron fueron la 1 (desaminaciĂłn espontĂĄnea de citosinas metiladas en sitios CpG), la 3 (fallo en la reparaciĂłn de la ruptura de la doble cadena de ADN mediante recombinaciĂłn homĂłloga), y la 9 (transveriones T> G en trinucleĂłtidos ApTpN y TpTpN), identificadas en el 96%, 54% y 46% de los pacientes, respectivamente. Respecto al repertorio de los genes de las Igs, se observĂł que el 26% de los pacientes con AL presentan mĂĄs de un clon, siendo esta heterogeneidad clonal similar a la encontrada en MM (23%). El gen IGHV3-30 fue identificado con mayor frecuencia tanto en AL como en MM, 10% y 12% de recurrencia, respectivamente.
Conclusiones: Este es el primer estudio de WES en una serie de pacientes con AL. Los resultados muestran que no hay una mutaciĂłn comĂșn driver en esta enfermedad, que podrĂan estar implicados mĂșltiples procesos mutacionales, y que los genes descritos mĂĄs frecuentemente mutados en AL y MM no coinciden. En conjunto, estos resultados suponen un avance en el entendimiento de la patogĂ©nesis de la AL
Prognostic utility of serum free light chain ratios and heavy-light chain ratios in multiple myeloma in three PETHEMA/GEM phase III clinical trials
We investigated the prognostic impact and clinical utility of serum free light chains (sFLC) and serum heavy-light chains (sHLC) in patients with multiple myeloma treated according to the GEM2005MENOS65, GEM2005MAS65, and GEM2010MAS65 PETHEMA/GEM phase III clinical trials. Serum samples collected at diagnosis were retrospectively analyzed for sFLC (n = 623) and sHLC (n = 183). After induction or autologous transplantation, 309 and 89 samples respectively were available for sFLC and sHLC assays. At diagnosis, a highly abnormal (HA) sFLC ratio (sFLCr) (32) was not associated with higher risk of progression. After therapy, persistence of involved-sFLC levels >100 mg/L implied worse survival (overall survival [OS], P = 0.03; progression-free survival [PFS], P = 0.007). Among patients that achieved a complete response, sFLCr normalization did not necessarily indicate a higher quality response. We conducted sHLC investigations for IgG and IgA MM. Absolute sHLC values were correlated with monoclonal protein levels measured with serum protein electrophoresis. At diagnosis, HA-sHLCrs (73) showed a higher risk of progression (P = 0.006). Additionally, involved-sHLC levels >5 g/L after treatment were associated with shorter survival (OS, P = 0.001; PFS, P = 0.018). The HA-sHLCr could have prognostic value at diagnosis; absolute values of involved-sFLC >100 mg/L and involved-sHLC >5 g/L could have prognostic value after treatment
Hadron shower decomposition in the highly granular CALICE analogue hadron calorimeter
The spatial development of hadronic showers in the CALICE scintillator-steel
analogue hadron calorimeter is studied using test beam data collected at CERN
and FNAL for single positive pions and protons with initial momenta in the
range from 10 to 80 GeV/c. Both longitudinal and radial development of hadron
showers are parametrised with two-component functions. The parametrisation is
fit to test beam data and simulations using the QGSP_BERT and FTFP_BERT physics
lists from Geant4 version 9.6. The parameters extracted from data and simulated
samples are compared for the two types of hadrons. The response to pions and
the ratio of the non-electromagnetic to the electromagnetic calorimeter
response, h/e, are estimated using the extrapolation and decomposition of the
longitudinal profiles.Comment: 38 pages, 19 figures, 5 tables; author list changed; submitted to
JINS
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