Building Intercultural Competence Through Intercultural Competency Certification Of Undergraduate Students

The Intercultural Competency Certificate (CCI in Spanish) designed for the Universidad Popular Automa del Estado de Puebla (UPAEP University) is a theory based comprehensive plan to develop undergraduate students intercultural competence. This Certificate is based in the Developmental Model of Intercultural Sensitivity (DMIS) developed by Milton Bennett (1993) and will be assessed by the Intercultural Development Inventory (IDI) v.3 developed by Bennett & Hammer (Hammer, 2009; Hammer, Bennett, & Wiseman, 2003). The main purpose of the Intercultural Competency Certificate is to develop students intercultural competence at least to the acceptance level of the DMIS continuum. To achieve this goal, the students will have to develop of necessary knowledge, skill and attitudes (Deardorff, 2006). This Certificate proposes to develop attitudes, knowledge and skills through certain activities as learning a new language, take international classes, reflect about intercultural differences, interact with people from other cultures, travel abroad and receive coaching by a professional who will guide them through tailor-make experiences according to the initial level of intercultural competence, measured by the IDI v.3 at the beginning of the Certification

Biorefinery of lemon peel waste using cold adapted yeasts from Antarctic and Sub-Antarctic regions

Cold adapted yeasts from soil samples from King George Island and Tierra del Fuego province were evaluated for their potential to produce extracellular pectinases. Pectinolytic yeasts were previously identified by 26S rDNA (D1/D2 domain) sequencing and phylogenetic analyses. Among 103 isolates, only eight showed pectinolytic activity at 20ºC, and only four -strains e9.2, 4.6, 5.9 and 8E- were capable to produce pectinolytic activity at 8ºC. Strain 8E identified as Guehomyces pullulans and the strains e9.2 and 5.9 identified as Cystofilobasidium infirmominiatum and Cryptoccocus adeliensis were selected for enzyme production under submerged fermentation. All the strains were capable to grow in presence of lemon peel. C. adeliensis 5.9 produced the highest enzyme activity at 24 h (4.8 U/ml) while C. infirmominiatum e9.2 and G. pullulans 8E showed considerable activity at 45 h (3.9 U/ml and 2.83 U/ml, respectively). It could be seen that at 10ºC enzyme/s remained active. Besides polygalacturonase (PGase), presence of other pectin-degrading enzymes in the culture supernatants was investigated. None of the strains produce neither pectin or pectate lyase activity nor rhamnogalacturonan hydrolase activity. Regarding pectin esterase activity, it was only produced by G. pullulans (0.022 U/ml). All the strains produced enzymatic pools that showed higher activity against highly esterified pectin than against pectin with 63% methoxyl. This behavior could be attributed to the presence of polymethylgalacturonase activity (PMGase) in its supernatant. β- glucosidase activity was detected in all supernatants. This is the first report on the capacity of these species to produce pectinases. Inulinase activity was detected in G. pullulans and C. infirmominiatum supernatants, while xylanase and cellulase activities were only detected in G. pullulans supernatants.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Biorefinery of lemon peel waste using cold adapted yeasts from Antarctic and Sub-Antarctic regions

Cold adapted yeasts from soil samples from King George Island and Tierra del Fuego province were evaluated for their potential to produce extracellular pectinases. Pectinolytic yeasts were previously identified by 26S rDNA (D1/D2 domain) sequencing and phylogenetic analyses. Among 103 isolates, only eight showed pectinolytic activity at 20ºC, and only four -strains e9.2, 4.6, 5.9 and 8E- were capable to produce pectinolytic activity at 8ºC. Strain 8E identified as Guehomyces pullulans and the strains e9.2 and 5.9 identified as Cystofilobasidium infirmominiatum and Cryptoccocus adeliensis were selected for enzyme production under submerged fermentation. All the strains were capable to grow in presence of lemon peel. C. adeliensis 5.9 produced the highest enzyme activity at 24 h (4.8 U/ml) while C. infirmominiatum e9.2 and G. pullulans 8E showed considerable activity at 45 h (3.9 U/ml and 2.83 U/ml, respectively). It could be seen that at 10ºC enzyme/s remained active. Besides polygalacturonase (PGase), presence of other pectin-degrading enzymes in the culture supernatants was investigated. None of the strains produce neither pectin or pectate lyase activity nor rhamnogalacturonan hydrolase activity. Regarding pectin esterase activity, it was only produced by G. pullulans (0.022 U/ml). All the strains produced enzymatic pools that showed higher activity against highly esterified pectin than against pectin with 63% methoxyl. This behavior could be attributed to the presence of polymethylgalacturonase activity (PMGase) in its supernatant. β- glucosidase activity was detected in all supernatants. This is the first report on the capacity of these species to produce pectinases. Inulinase activity was detected in G. pullulans and C. infirmominiatum supernatants, while xylanase and cellulase activities were only detected in G. pullulans supernatants.Centro de Investigación y Desarrollo en Fermentaciones Industriale

Helios 3198 solar simulator adaptation for the characterization of LCPV prototypes

This paper covers the adaptation and experimental verification that has been carried out to the Helios 3198 solar simulator to use it in the characterization of a LCPV prototype. The challenge is to deal with the diffuse light that can distort the measurement of a LCPV prototype because of the larger acceptance angle. A deflector chamber for the flash lamp has been designed and implemented to capture this diffuse light. The experimental verification has proven the reliability of the solution, demonstrating that the inclusion of this element in the equipment is not modifying the spatial uniformity and the angular distribution of the light at the receiver plane. Therefore, this work demonstrates that the Helios 3198 solar simulator, including a deflector chamber for the lamp, can be used for the characterization of a LCPV prototype

Experiencia inicial con la prótesis de despliegue rápido en posición aórtica Edwards Intuity

ResumenObjetivoDisponemos de una nueva clase de prótesis aórticas de despliegue rápido para tratamiento quirúrgico de la estenosis aórtica. Permitirían disminuir los tiempos quirúrgicos y facilitarían la cirugía mínimamente invasiva. Presentamos nuestra experiencia inicial con la válvula Edwards Intuity en el contexto del estudio multicéntrico Foundation.Pacientes y métodosEntre septiembre de 2012 y febrero de 2014 se implantaron 25 prótesis aórticas Edwards Intuity en 26 pacientes (77±4,3años; 52% varones). Veinticuatro (96%) fueron reemplazo valvular aislado, y 9 pacientes (36%) tuvieron abordaje mínimamente invasivo. Los pacientes fueron estudiados al alta, a los 3meses y al año de la intervención.ResultadosHubo éxito en el implante en el 96,2% de los pacientes (25/26). No hubo mortalidad hospitalaria. Seguimiento medio de 11±5,4meses, supervivencia acumulada del 96%. Un paciente falleció a los 11meses por un ictus isquémico. El tiempo medio de pinzamiento aórtico fue de 38,2±10,1min y el tiempo medio de circulación extracorpórea, de 62,4±11,1min. A los 3meses, el área valvular efectiva media fue de 1,70±0,42cm2. El 91% de los pacientes (23/25) estaban en grado funcionali en el seguimiento.ConclusionesEl implante de la válvula Edwards Intuity para el tratamiento de la estenosis aórtica ha sido factible, seguro y eficaz. Los tiempos de isquemia miocárdica y de circulación extracorpórea parecen reducirse en comparación con la cirugía valvular aórtica habitual. El comportamiento hemodinámico inicial de la prótesis Edwards Intuity es excelente.AbstractObjectiveA new class of rapid deployment prostheses is now available for surgical treatment of aortic stenosis. These prostheses offer the possibility of reducing the duration of cardiopulmonary bypass and aortic clamping times, and facilitate the use of minimally invasive surgery. We present our initial experience with the Edwards Intuity valve in the context of a multicentre Foundation study.Patients and methodsBetween September 2012 and February 2014, 25 Edwards Intuity valves were implanted in 26 patients (77±4.3years; 52% male). Twenty-four (96%) were isolated aortic valve replacement; a minimally invasive approach was used on 9 patients (36%). Patients were studied at discharge, three months, and one year after surgery.ResultsImplantation success rate was 96.2% (25/26). There was no hospital mortality. Mean follow-up of 11±5.54months, cumulative survival of 96%. One patient died eleven months after surgery due to ischaemic stroke. The mean aortic clamping time was 38.2±10.1minutes, and the mean cardiopulmonary bypass (CPB) time was 62.4±11.1minutes. In the echocardiography at 3months, the mean effective valve area was 1.70±0.42cm2, and 91% of patients (23/25) were in functional classi NYHA at follow-up.ConclusionsThe implantation of the Edwards Intuity valve for surgical treatment of aortic stenosis has shown to be feasible, safe and effective. Times of myocardial ischaemia and extracorporeal circulation appear to be reduced compared with normal aortic valve surgery. The initial haemodynamic performance of the Edwards Intuity prosthesis is excellent

A voxel-wise assessment of growth differences in infants developing autism spectrum disorder

Autism Spectrum Disorder (ASD) is a phenotypically and etiologically heterogeneous developmental disorder typically diagnosed around 4 years of age. The development of biomarkers to help in earlier, presymptomatic diagnosis could facilitate earlier identification and therefore earlier intervention and may lead to better outcomes, as well as providing information to help better understand the underlying mechanisms of ASD. In this study, magnetic resonance imaging (MRI) scans of infants at high familial risk, from the Infant Brain Imaging Study (IBIS), at 6, 12 and 24 months of age were included in a morphological analysis, fitting a mixed-effects model to Tensor Based Morphometry (TBM) results to obtain voxel-wise growth trajectories. Subjects were grouped by familial risk and clinical diagnosis at 2 years of age. Several regions, including the posterior cingulate gyrus, the cingulum, the fusiform gyrus, and the precentral gyrus, showed a significant effect for the interaction of group and age associated with ASD, either as an increased or a decreased growth rate of the cerebrum. In general, our results showed increased growth rate within white matter with decreased growth rate found mostly in grey matter. Overall, the regions showing increased growth rate were larger and more numerous than those with decreased growth rate. These results detail, at the voxel level, differences in brain growth trajectories in ASD during the first years of life, previously reported in terms of overall brain volume and surface area

Prácticas de física : laboratorio II

1 archivo PDF (86 páginas)Manual de laboratorio de prácticas de física

Crosstalk Between LXR and Caveolin-1 Signaling Supports Cholesterol Efflux and Anti-Inflammatory Pathways in Macrophages

© 2021 Ramírez, Torrecilla-Parra, Pardo-Marqués, de-Frutos, Pérez-García, Tabraue, de la Rosa, Martín-Rodriguez, Díaz-Sarmiento, Nuñez, Orizaola, Través, Camps, Boscá and Castrillo.Macrophages are immune cells that play crucial roles in host defense against pathogens by triggering their exceptional phagocytic and inflammatory functions. Macrophages that reside in healthy tissues also accomplish important tasks to preserve organ homeostasis, including lipid uptake/efflux or apoptotic-cell clearance. Both homeostatic and inflammatory functions of macrophages require the precise stability of lipid-rich microdomains located at the cell membrane for the initiation of downstream signaling cascades. Caveolin-1 (Cav-1) is the main protein responsible for the biogenesis of caveolae and plays an important role in vascular inflammation and atherosclerosis. The Liver X receptors (LXRs) are key transcription factors for cholesterol efflux and inflammatory gene responses in macrophages. Although the role of Cav-1 in cellular cholesterol homeostasis and vascular inflammation has been reported, the connection between LXR transcriptional activity and Cav-1 expression and function in macrophages has not been investigated. Here, using gain and loss of function approaches, we demonstrate that LXR-dependent transcriptional pathways modulate Cav-1 expression and compartmentation within the membrane during macrophage activation. As a result, Cav-1 participates in LXR-dependent cholesterol efflux and the control of inflammatory responses. Together, our data show modulation of the LXR-Cav-1 axis could be exploited to control exacerbated inflammation and cholesterol overload in the macrophage during the pathogenesis of lipid and immune disorders, such as atherosclerosis.We thank MINECO FPI predoctoral fellowship granted to MCO (BES-2015-075339). Experimental work was supported by grants from Ministerio de Ciencia, Investigación y Universidades, y Fondo Europeo de Desarrollo Regional (FEDER) Grant REF: PID2019-104284RB-I00/AEI/10.13039/501100011033 (to AC) and support from Networks of Excellence from MINECO (Nuclear Receptors in Cancer, Metabolism and Inflammation [NuRCaMeIn] SAF2017-90604-REDT to AC. Ministerio de Economía, Industria y Competitividad, Ministerio de Ciencia, Investigación y Universidades, and Agencia Estatal de Investigación (SAF2017-82436-R, RTC2017-6283-1), Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares (CB16/11/00222), Consorcio de Investigación en Red de la Comunidad de Madrid, S2017/BMD-3686 and Fondo Europeo de Desarrollo Regional (to LB). Ministerio de Ciencia, Investigación y Universidades, and Agencia Estatal de Investigación Proyectos de I+D+i Retos Investigación 2018 (RTI2018-095061-B-I00); TALENTO Grant from Madrid Government, Spain (2017-T1/BMD-5333); Consejería de Ciencia, Universidades e Innovación Comunidad de Madrid, Spain (PEJD-2018-POST/BMD-8900 and PEDJ-2018-AI/BDM-9724) to CMR

Análise do genoma completo para identificar variações associadas à circunferência escrotal em bovinos Charolês

The scrotal circumference is one of the main characteristics related to fertility of the bovine male and female. Its study through genetic and genomic tools would help to the discovery of favorable variation and genetic architecture to promote its selection. The aim of this work was to carry out a genome- wide association study using a large microarray of SNPs, to identify variations and candidate genes associated with the studied trait, for which information from 141 Charolais bulls was used. Four markers found, rs110416965, rs110130953, rs43423602 and rs29003417, showed significant association at the genome level. Three of them are found within candidate genes (RBFOX2, TTF2 and CXCL2). This information contributes to the understanding of the genetic architecture of this trait.  La circunferencia escrotal es una de las características realcionadas a la fertilidad del macho y la hembra bovina. Su estudio a través de herramientas genéticas y genómicas ayudaría al descubrimiento de variación favorable en su arquitectura genética para fomentar su selección. El propósito del presente trabajo fue realizar un estudio de genoma completo mediante un microarreglo de SNPs, para identificar variaciones y genes candidatos asociados a la característica, para lo que se utilizó la información de 141 toros de la raza Charolais. Cuatro marcadores encontrados, rs110416965, rs110130953, rs43423602 y rs29003417, mostraron asociación significativa a nivel de genoma. Tres de ellos se encuentran dentro de genes candidatos (RBFOX2, TTF2 y CXCL2) para ser estudiados más especificamente. Esta información contribuye al entendiemiento de la arquitectura genética de esta característicaA circunferência escrotal é uma das características relacionadas à fertilidade do macho e da fêmea bovina. Seu estudo por meio de ferramentas genéticas e genômicas auxiliaria na descoberta de variação favorável em sua arquitetura genética para promover sua seleção. O objetivo deste trabalho foi realizar um estudo do genoma completo usando um microarray de SNPs, para identificar variações e genes candidatos associados à característica, para o qual foram utilizadas informações de 141 touros Charolês. Quatro marcadores encontrados, rs110416965, rs110130953, rs43423602 e rs29003417, mostraram associação significativa no nível do genoma. Três deles foram encontrados em genes candidatos (RBFOX2, TTF2 e CXCL2) a serem estudados mais especificamente. Essas informações contribuem para o entendimento da arquitetura genética dessa característica

A voxel-wise assessment of growth differences in infants developing autism spectrum disorder

Autism Spectrum Disorder (ASD) is a phenotypically and etiologically heterogeneous developmental disorder typically diagnosed around 4 years of age. The development of biomarkers to help in earlier, presymptomatic diagnosis could facilitate earlier identification and therefore earlier intervention and may lead to better outcomes, as well as providing information to help better understand the underlying mechanisms of ASD. In this study, magnetic resonance imaging (MRI) scans of infants at high familial risk, from the Infant Brain Imaging Study (IBIS), at 6, 12 and 24 months of age were included in a morphological analysis, fitting a mixed-effects model to Tensor Based Morphometry (TBM) results to obtain voxel-wise growth trajectories. Subjects were grouped by familial risk and clinical diagnosis at 2 years of age. Several regions, including the posterior cingulate gyrus, the cingulum, the fusiform gyrus, and the precentral gyrus, showed a significant effect for the interaction of group and age associated with ASD, either as an increased or a decreased growth rate of the cerebrum. In general, our results showed increased growth rate within white matter with decreased growth rate found mostly in grey matter. Overall, the regions showing increased growth rate were larger and more numerous than those with decreased growth rate. These results detail, at the voxel level, differences in brain growth trajectories in ASD during the first years of life, previously reported in terms of overall brain volume and surface area