A genetic network that suppresses genome rearrangements in Saccharomyces cerevisiae and contains defects in cancers.

Gross chromosomal rearrangements (GCRs) play an important role in human diseases, including cancer. The identity of all Genome Instability Suppressing (GIS) genes is not currently known. Here multiple Saccharomyces cerevisiae GCR assays and query mutations were crossed into arrays of mutants to identify progeny with increased GCR rates. One hundred eighty two GIS genes were identified that suppressed GCR formation. Another 438 cooperatively acting GIS genes were identified that were not GIS genes, but suppressed the increased genome instability caused by individual query mutations. Analysis of TCGA data using the human genes predicted to act in GIS pathways revealed that a minimum of 93% of ovarian and 66% of colorectal cancer cases had defects affecting one or more predicted GIS gene. These defects included loss-of-function mutations, copy-number changes associated with reduced expression, and silencing. In contrast, acute myeloid leukaemia cases did not appear to have defects affecting the predicted GIS genes

Análise de rótulos de BCAA comercializados no município de Volta Redonda-RJ

Os BCAA são substâncias formadas a partir da conjugação de leucina, isoleucina e valina. No Brasil, segundo a ANVISA, dispensam registros por não possuírem legislação específica. Todavia, devem cumprir requisitos de composição e qualidade estabelecidos pelos regulamentos técnicos. O presente estudo teve como objetivo avaliar, segundo parâmetros regulamentares da ANVISA, os rótulos dos produtos comercializados como aminoácidos de cadeia ramificada - BCAA. Trata-se de um estudo observacional, onde os produtos foram selecionados em uma loja especializada em comercialização de ergogênicos nutricionais na cidade de Volta Redonda. Todos os produtos comercializados com a descrição BCAA tiveram suas embalagens avaliadas segundo requisitos técnicos específicos das Resoluções nº 18, de 27 de Abril de 2010, nº 23, de 15 de março de 2000 e Portaria SVS/MS nº 222, de 24 de março de 1998. Para a tabulação dos resultados, foram utilizados modelos clássicos de análises estatísticas descritivas. A maioria dos produtos avaliados estava em conformidade com a legislação brasileira pertinente a comercialização de BCAA, apresentando 92,6% de conformidades. Apesar de ainda não terem comprovação científica a cerca de seus benefícios, os BCAA são muito utilizados no meio esportivo, logo, há necessidade de fiscalização destes, de forma a adequá-los as exigências propostas pela ANVISA. ABSTRACTAnalysis of BCAA labels sold in Volta Redonda-RJThe BCAA are substances formed from the combination of leucine, isoleucine and valine. In Brazil according to ANVISA, they needn’t records because of lack of specific legislation. However, it’s must comply with the composition and quality requirements established by technical regulations. The present study aimed to evaluate according ANVISA regulatory parameters the labels of the products marketed as branched chain amino acids - BCAA. This is an observational study. The products were selected from a store specializing in nutritional ergogenics. All BCAA had her evaluated packages according to resolutions of ANVISA. To tabulate the results, we used classical descriptive statistical analysis models. Most products were evaluated in accordance with the relevant marketing BCAA Brazilian’s law, with 92.6 % compliance with the legislation of the information. Although not yet scientifically proven, BCAAs are highly used in sports. It is necessary to monitor of use in order to adapt them the requirements proposed by ANVISA

Bone resorption is affected by follicular phase length in female rotating shift workers.

Stressors as subtle as night work or shift work can lead to irregular menstrual cycles, and changes in reproductive hormone profiles can adversely affect bone health. This study was conducted to determine if stresses associated with the disruption of regular work schedule can induce alterations in ovarian function which, in turn, are associated with transient bone resorption. Urine samples from 12 rotating shift workers from a textile mill in Anqing, China, were collected in 1996-1998 during pairs of sequential menstrual cycles, of which one was longer than the other (28.4 vs. 37.4 days). Longer cycles were characterized by a prolonged follicular phase. Work schedules during the luteal-follicular phase transition (LFPT) preceding each of the two cycles were evaluated. All but one of the shorter cycles were associated with regular, forward phase work shift progression during the preceding LFPT. In contrast, five longer cycles were preceded by a work shift interrupted either by an irregular shift or a number of "off days." Urinary follicle-stimulating hormone levels were reduced in the LFPT preceding longer cycles compared with those in the LFPT preceding shorter cycles. There was greater bone resorption in the follicular phase of longer cycles than in that of shorter cycles, as measured by urinary deoxypyridinoline. These data confirm reports that changes in work shift can lead to irregularity in menstrual cycle length. In addition, these data indicate that there may be an association between accelerated bone resorption in menstrual cycles and changes of regularity in work schedule during the preceding LFPT

Proposal of a Model for IT Service Continuity Management

Micro and small companies in the software area now represent more than 90% of organizations in the country and grow 6% annually on average. However, the lack of planning in these companies in business continuity management contributes to their mortality when problems occur, such as disasters and disruption of system services. The implementation of IT Service Continuity Management as a tool for planning would be the solution. This research presents the current level of maturity of these companies in a Micro scenario and Small IT Companies in Paraná, at Londrina and Cascavel region

Contribution of microscopy for understanding the mechanism of action against trypanosomatids

Transmission electron microscopy (TEM) has proved to be a useful tool to study the ultrastructural alterations and the target organelles of new antitrypanosomatid drugs. Thus, it has been observed that sesquiterpene lactones induce diverse ultrastructural alterations in both T. cruzi and Leishmania spp., such as cytoplasmic vacuolization, appearance of multilamellar structures, condensation of nuclear DNA, and, in some cases, an important accumulation of lipid vacuoles. This accumulation could be related to apoptotic events. Some of the sesquiterpene lactones (e.g., psilostachyin) have also been demonstrated to cause an intense mitochondrial swelling accompanied by a visible kinetoplast deformation as well as the appearance of multivesicular bodies. This mitochondrial swelling could be related to the generation of oxidative stress and associated to alterations in the ergosterol metabolism. The appearance of multilamellar structures and multiple kinetoplasts and flagella induced by the sesquiterpene lactone psilostachyin C indicates that this compound would act at the parasite cell cycle level, in an intermediate stage between kinetoplast segregation and nuclear division. In turn, the diterpene lactone icetexane has proved to induce the external membrane budding on T. cruzi together with an apparent disorganization of the pericellar cytoskeleton. Thus, ultrastructural TEM studies allow elucidating the possible mechanisms and the subsequent identification of molecular targets for the action of natural compounds on trypanosomatids.Fil: Lozano, Esteban Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Spina Zapata, Renata María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Anti-inflammatory and antinociceptive activities of Campomanesia adamantium.

Ethnopharmacological relevance: Campomanesia species are used in folk medicine as anti-in?ammatory, anti-rheumatic, anti-diarrheal and hypocholesterolemic. Aim of the study: The present study investigated the in vivo anti-in?ammatory and antinociceptive properties of ethyl acetate (AE) and aqueous (Aq) extracts from leaves of Campomanesia adamantium and in vitro anti-in?ammatory activity of AE and its isolated ?avonols, myricitrin and myricetin. Materials and methods: The antinociceptive activity of AE and Aq was evaluated using acetic acid- induced writhing and formalin methods. The in vivo anti-in?ammatory effect of AE and Aq was evaluated using carrageenan-induced paw oedema in mice. AE, myricitrin and myricetin were evaluated for their abilities to modulate the production of NO, TNF-a and IL-10 in LPS/IFN-g stimulated J774.A1 macrophages. Results: It was found that orally administrated AE and Aq (125 and 250 mg/kg) inhibited carrageenan- induced paw oedema in mice. AE (125 and 250 mg/kg) and Aq (125 mg/kg) reduced the time to licking at the second phase of the formalin method in vivo in mice. AE (250 mg/kg) and Aq (125 mg/kg) also reduced the number of writhes. AE, myricitrin and myricetin inhibited NO (320 mg/mL and 6.25?100 mM, respectively) and TNF-a production by macrophages (320 mg/mL for AE, 100 mM for myricitrin and 25?100 mM for myricetin). AE (160 and 320 mg/mL), myricitrin (50 and 100 mM) and myricetin (25?100 mM) increased IL-10 production by macrophages. Conclusions: The ethyl acetate and aqueous extracts from Campomanesia adamantium showed anti- nociceptive and anti-in?ammatory effects supporting the use of the plant in folk medicine. The results suggest that anti-oedematogenic effect promoted by aqueous extract involves several anti- in?ammatory mechanisms of action. The antinociceptive effect shown by aqueous extract can be due to the modulation of release of in?ammatory mediators involved in nociception. The anti-in?ammatory effects of AE and of its isolated ?avonols may be attributed to inhibition of pro-in?ammatory cytokines production, TNF-a and NO and to the increased of IL-10 production

Efeito de diferentes tratamentos t?rmicos sobre microestrutura e microdureza de um sistema a?o C-Mn/revestimento de inconel 625.

A utiliza??o de tubos de a?o C-Mn na ind?stria de ?leo e g?s ? muito comum desde o nascimento desta ind?stria. Mesmo com a grande evolu??o da metalurgia destes a?os ao longo das ?ltimas d?cadas, o desafio de desempenho em ambientes severos de corros?o devido ?s caracter?sticas reativas (H2S, CO2) do petr?leo e seus derivados, imp?em uma nova era para a fabrica??o de tubos. A solu??o tecnol?gica mais direta seria a utiliza??o de a?os com alta liga (Ni, Cr) e/ou superligas de n?quel. No entanto, os custos de produ??o tornariam invi?veis pelo alto custo destes materiais. Neste contexto, ? crescente iniciativas de pesquisas para o desenvolvimento de overlay e/ou cladding, ou seja, revestimentos de a?os C-Mn usando superligas de n?quel e a?os inoxid?veis. Neste trabalho, foi utilizada a deposi??o de uma camada da superliga de n?quel Inconel 625 atrav?s do processo laser. Uma das desvantagens da deposi??o a laser ou qualquer outro processo de fus?o ? a forma??o de uma regi?o de descontinuidade microestrutural no a?o, chamada de ZTA (zona termicamente afetada pelo calor). Neste estudo, diferentes rotas de tratamentos t?rmicos foram investigadas com o objetivo de eliminar a ZTA e preservar as caracter?sticas originais do substrato e do revestimento.The application of C-Mn steel pipe in the oil & gas industry is quite common since starting of this industry. Even with the great evolution of the metallurgy of this type of steel over the last decades, the challenge is to overcome the corrosion severity caused by sour (H2S, CO2) species present in the petroleum and its derivates. The direct technological solution would be to replace the C-Mn steel by Ni-based superalloys and/or stainless steels. However, the high costs of these materials would make several projects impracticable. In this context, there are several initiatives in order to develop overlay and/or cladding, that means, coatings of C-Mn steels using superalloys and stainless steels. In this work, it was used an overlay deposition of Inconel 625 on the surface of a C-Mn steel using the laser process. A disadvantage of laser deposition, as it is for any deposition melting process, is the formation of a microstructural discontinuity in the steel substrate caused by the thermal cycle (HAZ = heat affected zone). In this study, different heat treatment routes were investigated aiming to eliminate the HAZ preserving the original characteristics of the substrate and of the coating

Estabilidade de caracter?sticas f?sico-qu?micas em sucos naturais de cenoura e laranja armazenados sob refrigera??o.

O objetivo do estudo foi avaliar o pH, acidez, atividade antioxidante e Vit C e suas estabilidades, durante o armazenamento sob refrigera??o em sucos de cenoura e laranja com suas misturas em propor??es espec?ficas feitas a cada tempo de medida. Cinco formula??es de suco foram preparadas com cenoura-laranja nas propor??es: 100:0 (100C), 25:75 (25C75L), 50:50 (50C50L), 75:25 (75L25) e 0:100 (100L) de sucos de cenoura e laranja, respectivamente. An?lise foi realizada em amostras de sucos em 7 tempos (0 a 12 h). Os valores de pH, acidez titul?vel para os extratos de 100C, 75C25L e 100L mostraram uma estabilidade no per?odo avaliado, e para os extratos de 50C50L e 25C75L houve queda significativa. A atividade antioxidante e Vit C permaneceram constante. Os resultados mostram que o os sucos frescos refrigerados podem ser consumidos ap?s 12 horas sem a perda de sua capacidade antioxidante. No entanto, para uma maior atividade antioxidante, recomenda-se o consumo do suco de laranja.Theobjective of this studywas to evaluate the pH, acidity,antioxidant activity and vit Cand their stabilityduring storageunder refrigerationin juicesof carrot,orange and their mixtures in specific proportions made at any time of measure.Five formulations of juicewere preparedwithcarrot- orange following proportions: 100:0(100C), 25:75(25C75L), 50:50(50C50L), 75:25(75L25) and 0:100 (100L) of carrot and orange juices, respectively. Evaluation was carried out injuices samples in 7times (0 to 12 h). The pH values, titratable acidity for extracts of 100C, 100L and 75C25L showed stability during the study period, and extracts 50C50L and 25C75L showed significant decrease. The antioxidant activity and vitamin C remained constant. The results show that fresh chilled juices can be consumed over a period of 12 hours without loss of their antioxidant capacity. However, for greater antioxidant activity, it is recommended the consumption of orange juice