Sporopollenin as an efficient green support for covalent immobilization of a lipase

Sporopollenin exine capsules (SECs), derived from the spores of Lycopodium clavatum, have been functionalised with 1,n-diamines and the resulting aminoalkyl microcapsules used to immobilize Candida antarctica lipase B (Cal B) via a glutaradehyde-based diimine covalent linker. The supported enzyme efficiently catalyzes the esterification of oleic acid with ethanol. Initial rates using the SEC-CalBs were comparable to the commercial enzyme Novozym 435, but displayed up to 20-fold higher specific activity. The supported enzymes could also be recycled and after four cycles displayed only a modest decrease in conversions. In a kinetic resolution the SEC-CalBs efficiently acetylated rac-1-phenylethanol, with conversions up to 37% after 5 hours and product enantiomeric excesses of >99%. Related to this, the dynamic resolution of rac-1-phenylethylamine, in the presence of Pd-BaSO₄ and ammonium formate, led to the acetylated amine with a 94% conversion and >99% ee

Essential role of the czc determinant for cadmium, cobalt and zinc resistance in Gluconacetobacter diazotrophicus PAl 5

The mechanisms of cadmium, cobalt and zinc resistance were characterized in the plant-growth-promoting bacterium Gluconacetobacter diazotrophicus PAl 5. The resistance level of the wild-type strain was evaluated through the establishment of minimum inhibitory concentrations (MIC) of the soluble compounds CdCl2·H2O, CoCl2·6H2O and ZnCl2. Gluconacetobacter diazotrophicus PAl 5 was resistant to high concentrations of Cd, Co and Zn, with MICs of 1.2, 20 and 20 mM, respectively. Screening of an insertion library from transposon EZ-Tn5<R6Kyori/KAN-2> in the presence of ZnO revealed that the mutant GDP30H3 was unable to grow in the presence of the compound. This mutant was also highly sensitive to CdCl2·H2O, CoCl2·6H2O and ZnCl2. Molecular characterization established that the mutation affected the czcA gene, which encodes a protein involved in metal efflux. In silico analysis showed that czcA is a component of the czcCBARS operon together with four other genes. This work provides evidence of the high tolerance of G. diazotrophicus PAl 5 to heavy metalsand that czc is a determinant for metal resistance in this bacterium. [Int Microbiol 2012; 15(2):69-78

Refractory pemphigus foliaceus associated with herpesvirus infection: case report

Pemphigus foliaceus (PF) is an autoimmune disease characterized by blistering of the skin. Infections caused by members of the herpesviridae family have been suggested as a possible triggering factor for pemphigus vulgaris (PV), but not for PF. The present study aimed to investigate the presence of Human herpesvirus (types 1, 2, 3) in corticosteroid refractory skin lesions from a patient with PF, by a Polymerase chain reaction (PCR) assay. The sample collected from cutaneous blisters has tested positive for herpes simplex virus type 1 (HSV1) after sequence analysis of the amplified viral genomic segment. The study concluded that when PF patients present corticosteroid or immunosuppressants refractory lesions, herpetic infection should be considered

Validación portuguesa del inventario de las prácticas creativas en la educación superior

Muito tem sido discutido acerca do papel da educação superior na formação de profissionais aptos a lidar de forma criativa com os desafios que o mundo contemporâneo lhes impõe. Práticas docentes para a promoção da criatividade na educação superior são um alvo a pesquisar, embora se verifique escassez de instrumentos de avaliação. O objetivo deste estudo foi adaptar e validar, para o contexto português, o Inventário de Práticas Docentes para a Criatividade na Educação Superior, desenvolvido no Brasil. Participaram da pesquisa 582 estudantes universitários. Os resultados apontam um instrumento que avalia quatro fatores (incentivo a novas ideias; clima para expressão de ideias; interesse pela aprendizagem do aluno; avaliação e metodologia de ensino). Suas características psicométricas, em termos de precisão e de validade, revelaram-se adequadas. Conclui-se que o instrumento constitui uma ferramenta útil para fins de pesquisa e diagnóstico de condutas docentes quanto ao estímulo à criatividade.It has been much discussed the role of the higher education in training professionals prepared to deal creatively with the challenges that the modern world imposes on them. Teaching practices for the promotion of creativity emerge as a target to be investigated, especially considering students’ perceptions. The purpose of this study was to validate and adapt, to the Portuguese context, the Teaching Practices for Creativity in Higher Education Inventory, developed in Brazil. Five hundred and eighty-two university students participated in the study. The results pointed an instrument that assesses four factors (Incentive to New Ideas, Climate for Expression of Ideas, Interest for Students’ Learning, and Evaluation and Teaching Methodology) and its psychometric properties, in terms of precision and validity, proved to be adequate. It is concluded that the instrument constitutes a useful instrument for research and diagnostic of teachers’ behaviors that foster creativity.Mucho se ha discutido acerca del papel de la educación superior en la formación de profesionales capaces de enfrentar creativamente los desafíos que el mundo moderno les impone. Las prácticas docentes para la creatividad surgen como una búsqueda de destino, especialmente teniendo en cuenta las percepciones de los estudiantes. El objetivo de este estudio fue validar y adaptar al contexto portugués, el Inventario de Práctica de Enseñanza para la Creatividad en la Educación Superior, desarrollado en Brasil. Los participantes fueron 582 estudiantes universitarios. Los resultados indican un instrumento con cuatro factores (Fomento de las Nuevas Ideas, Clima para la Expresión de Ideas, Interés en el Aprendizaje Estudiantil y Evaluación y Metodología de la Enseñanza) y sus propiedades psicométricas, en términos de precisión y validez, resultó adecuada. El instrumento es una herramienta útil para la investigación y el diagnóstico de la conducta de los profesores ante el estímulo a la creatividad.Universidade do MinhoTorrance CenterUniversidade de Brasíli

Straight to the Point:Fast-forwarding Videos via Reinforcement Learning Using Textual Data

The rapid increase in the amount of published visual data and the limited time of users bring the demand for processing untrimmed videos to produce shorter versions that convey the same information. Despite the remarkable progress that has been made by summarization methods, most of them can only select a few frames or skims, which creates visual gaps and breaks the video context. In this paper, we present a novel methodology based on a reinforcement learning formulation to accelerate instructional videos. Our approach can adaptively select frames that are not relevant to convey the information without creating gaps in the final video. Our agent is textually and visually oriented to select which frames to remove to shrink the input video. Additionally, we propose a novel network, called Visually-guided Document Attention Network (VDAN), able to generate a highly discriminative embedding space to represent both textual and visual data. Our experiments show that our method achieves the best performance in terms of F1 Score and coverage at the video segment level

Irradiação de micro-ondas aplicada à síntese orgânica: uma história de sucesso no Brasil

This review article shows the publications in the field of microwave irradiation published by Brazilian researchers over the past 10 years. In South America, Brazil leads the publication of articles with the use of microwave irradiation possessing a large advantage in number of articles published over the others countries. The works were divided into four major areas: Catalysis, Reactions without Solvent, Heterocycles Chemistry, Natural Products and Other, and some could be classified in more than one category

Práticas Docentes para Criatividade na Universidade: Estudo em Portugal e no Brasil

Creativity is nowadays seen as an essential feature in higher education. Nevertheless, there is a discrepancy between the need for creativity and what higher education classrooms provide. This study assessed the perceptions of 1599 higher education students from two countries (1059 Brazilian and 540 Portuguese students), from two academic domains (Sciences and Technologies - Sc&T; Social Sciences, Arts, and Humanities - SScA&H), about the presence of creativity in their teachers’ instruction and evaluation practices. The study’s findings evidence interactive effects between the variables country and academic domain for most of the assessed factors: encouragement of new ideas, climate for the expression of ideas, and interest in students’ learning. Brazilian Sc&T students presented more negative perceptions of their classroom environments when compared to SScA&H students; Portuguese students showed opposite patterns of results. Some hypothetical explanations are discussed and future directions for research are presented.La creatividad está actualmente considerada como aspecto esencial en la Educación Superior. Sin embargo, existe discrepancia entre la necesidad de creatividad y lo que la universidad ofrece. Este estudio evaluó percepciones de 1.599 estudiantes universitarios (1.059 de Brasil y 540 de Portugal) de dos áreas curriculares (Ciencia y Tecnología - Sc&T; Ciencias Sociales, Artes y Humanidades - SscA&T) acerca de la presencia de creatividad en las prácticas docentes, instructivas y evaluativas dirigidas a ellos. Los resultados mostraron efectos de interacción significativos entre las variables país y área curricular para la mayoría de los factores evaluados: fomento de nuevas ideas, entorno para la expresión de ideas e interés en el aprendizaje del estudiante. Los estudiantes brasileños de Sc&T mostraron percepciones más negativas de la clase en comparación con los de SSCA&H; los estudiantes portugueses obtuvieron patrones opuestos en los resultados. Algunas hipótesis explicativas se discuten y se presentan directrices para investigación futura.Criatividade é atualmente tomada como aspecto essencial na Educação Superior. Há, contudo, discrepância entre a necessidade de criatividade e o que a universidade proporciona. Este estudo avaliou percepções de 1599 alunos universitários de dois países (1059 brasileiros e 540 portugueses), de duas áreas curriculares (Ciências e Tecnologias - Sc&T; Ciências Sociais, Artes e Humanidades - SscA&H) sobre a presença de criatividade nas práticas docentes, instrucionais e avaliativas, de que são alvo. Os resultados mostraram efeitos interativos significativos entre as variáveis país e área curricular para a maioria dos fatores avaliados: encorajamento de novas ideias, clima para expressão de ideias e interesse pela aprendizagem dos alunos. Os estudantes brasileiros de Sc&T mostraram percepções mais negativas da sala de aula, comparados com os de SScA&H; os alunos portugueses obtiveram padrões opostos nos resultados. Algumas hipóteses explicativas são discutidas e são apresentadas orientações para pesquisa futura