23,503 research outputs found

    C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)

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    Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A. mixtus, A. sepulcralis and A. macraspis. The species have holokinetic chromosomes which is typical of the Hemiptera, and their diploid chromosome number is 2n = 14 and they have an XX/XY (female/male) sex chromosome system. C-banding revealed telomeric blocks of constitutive heterochromatin in most chromosomes of these three species. In each species, CMA;-positive blocks were observed in the telomeric heterochromatin region of chromosome pair 1. This chromosome pair carried the nucleolar organizer region (NOR), which silver nitrate staining revealed is located in the telomeric heterochromatin. In contrast to the result obtained with CMA3, entire chromosome complement stained homogenously with DAPI, except for the telomeric region of pair 1, which remained unstained.Fil: Lanzone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidade Federal de Pernambuco; BrasilFil: de Souza, Maria José. Universidade Federal de Pernambuco; Brasi

    Chromosome complement and meiosis in three species of the Neotropical bug genus Antiteuchus (Heteroptera, Pentatomidae, Discocephalinae)

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    Orcein staining of spermatocytes was used to study the meiotic behavior of holocentric chromosomes in three member of the genus Antiteuchus (commonly known as stink bugs). We describe and illustrate the karyotype of Antiteuchus mixtus, A. sepulcralis and A. macraspis which were cytogenetically characterized as having a diploid number of 2n = 14 and an XY sex chromosome system showing pre-reductional meiosis for autosomes and post-reductional meiosis for sex chromosomes. These species were also shown to have a long diffuse stage during meiotic prophase I and aberrant harlequin-type meiocytes. The chiasma frequency was also analyzed for two of the three species studied.Fil: Lanzone, Cecilia. Universidade Federal de Pernambuco; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: de Souza, Maria José. Universidade Federal de Pernambuco; Brasi

    STRATEGY AS DISCURSIVE PRACTICE IN A BRAZILIAN PUBLIC UNIVERSITY: A LOOK UNDER THE PERSPECTIVE OF CRITICAL DISCOURSE ANALYSIS

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    The aim this article a critical discursive analysis of the “management plan” genre of a public institution of higher education, from 2012 to 2015, located in southeast Brazil. The aforementioned plan is inserted in the discursive practice of strategic management, specifically the institutional, bureaucratic management, and is used as an instrument for decision-making. The goal of this analysis will be to discuss the first step of the “management plan”, named “organizational policies”. We can see that, while elaborating declarative sentences, there is an evaluation of the statements regarding what is to be considered relevant to the institutions by means of the ideological discourse on neoliberal ideals and market behavior. The adoption of market-oriented managerial tools has been a constant in public administration. The public administration looks for bases of organizational practices in the private sphere. This mimicry is still present in the field, and the search for new managerial practices still crosses the imaginaries of the public managers. However, the increasing incorporation of a market-oriented, neoliberal logic, mainly in the adoption of strategic planning, can still be verified. The conclusion presented in this paper serves to foment the debate on the strategies formulated for the Brazilian public service and the methodological applicability of the critical discourse analysis. This meets the emerging need to systematize and integrate distinct theoretical and methodological approaches in the field of organizational studies when strategy is studied as a social and discursive practice

    A reforma agrária no segundo mandato de Fernando Henrique Cardoso.

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    Já no primeiro mandato do presidente Fernando Henrique Cardoso, a política agrária sofreu reorientações significativas, que marcariam seu segundo mandato, a partir da nomeação de Raul Jungmann como ministro do Desenvolvimento Agrário. A reforma agrária finalmente encontrou o seu sujeito, a agricultura familiar, e o Estado encontrou a sua missão agrária como gestor do território. Foram assim estabelecidas as bases concretas para o exercício da função social da propriedade, recuperando o Estado, na prática, o domínio eminente do território do país. Importante providência nesse sentido foi a anulação dos títulos irregulares de propriedade de sessenta milhões de hectares de terra. O governo firmou a prerrogativa do Estado de "rotinizar" a redistribuição da terra concentrada e regular e assegurar o direito de propriedade em associação com a dupla alternativa de dois modelos agrícolas conviventes: o agronegócio e a agricultura familiar

    Germano José de Souza

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    O presente artigo discorre sobre uma visão holística das Universidades sobre o ensino superior no Brasil, através do Programa “Universidade para Todos - ProUni. Tem como principal tema estabelecer cotas de bolsas de estudos, para a inclusão de alunos carentes, provenientes de escolas públicas e privadas, em troca de isenção fiscal. As referidas cotas, além de atender os alunos carentes, também favorecem a classe da raça negra, indígena e portadores de necessidades especiais. O objetivo principal do artigo é apontar uma relação histórica comparativa, demonstrando inicialmente, uma total exclusão dos mesmos na Universidade

    Visualizing test diversity to support test optimisation

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    Diversity has been used as an effective criteria to optimise test suites for cost-effective testing. Particularly, diversity-based (alternatively referred to as similarity-based) techniques have the benefit of being generic and applicable across different Systems Under Test (SUT), and have been used to automatically select or prioritise large sets of test cases. However, it is a challenge to feedback diversity information to developers and testers since results are typically many-dimensional. Furthermore, the generality of diversity-based approaches makes it harder to choose when and where to apply them. In this paper we address these challenges by investigating: i) what are the trade-off in using different sources of diversity (e.g., diversity of test requirements or test scripts) to optimise large test suites, and ii) how visualisation of test diversity data can assist testers for test optimisation and improvement. We perform a case study on three industrial projects and present quantitative results on the fault detection capabilities and redundancy levels of different sets of test cases. Our key result is that test similarity maps, based on pair-wise diversity calculations, helped industrial practitioners identify issues with their test repositories and decide on actions to improve. We conclude that the visualisation of diversity information can assist testers in their maintenance and optimisation activities

    Genomic selection in rubber tree breeding: A comparison of models and methods for managing G×E interactions

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    Several genomic prediction models combining genotype × environment (G×E) interactions have recently been developed and used for genomic selection (GS) in plant breeding programs. G×E interactions reduce selection accuracy and limit genetic gains in plant breeding. Two data sets were used to compare the prediction abilities of multienvironment G×E genomic models and two kernel methods. Specifically, a linear kernel, or GB (genomic best linear unbiased predictor [GBLUP]), and a nonlinear kernel, or Gaussian kernel (GK), were used to compare the prediction accuracies (PAs) of four genomic prediction models: 1) a single-environment, main genotypic effect model (SM); 2) a multienvironment, main genotypic effect model (MM); 3) a multienvironment, single-variance G×E deviation model (MDs); and 4) a multienvironment, environment-specific variance G×E deviation model (MDe). We evaluated the utility of genomic selection (GS) for 435 individual rubber trees at two sites and genotyped the individuals via genotyping-by-sequencing (GBS) of single-nucleotide polymorphisms (SNPs). Prediction models were used to estimate stem circumference (SC) during the first 4 years of tree development in conjunction with a broad-sense heritability (H2) of 0.60. Applying the model (SM, MM, MDs, and MDe) and kernel method (GB and GK) combinations to the rubber tree data revealed that the multienvironment models were superior to the single-environment genomic models, regardless of the kernel (GB or GK) used, suggesting that introducing interactions between markers and environmental conditions increases the proportion of variance explained by the model and, more importantly, the PA. Compared with the classic breeding method (CBM), methods in which GS is incorporated resulted in a 5-fold increase in response to selection for SC with multienvironment GS (MM, MDe, or MDs). Furthermore, GS resulted in a more balanced selection response for SC and contributed to a reduction in selection time when used in conjunction with traditional genetic breeding programs. Given the rapid advances in genotyping methods and their declining costs and given the overall costs of large-scale progeny testing and shortened breeding cycles, we expect GS to be implemented in rubber tree breeding programs
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