Screening of Bacillus sp. isolated from coral Siderastrea stellata for antimicrobial activity against enteropathogenic strains of Salmonella and Escherichia coli / Screening de Bacillus sp. isolados do coral Siderastrea stellata para atividade antimicrobiana contra linhagens enteropatogênicas de Salmonella e Escherichia coli

Siderastrea stellata is a coral endemic to Brazilian waters and is widely distributed throughout the coast of Bahia state. Exposure of the coral to pathogens stimulates the production of important enzymes by associated symbiont microorganisms. Besides being natural sources of bioactive compounds, these microorganisms possess characteristics that help them survive under extreme conditions. Sixty-eight bacterial strains isolated from the S. stellata coral were analyzed for antimicrobial activity against ten pathogenic bacteria. The cultivation of marine isolates was carried out in liquid or solid seawater. Eight isolates produced antimicrobial compounds against at least two of the ten pathogenic bacteria tested. When isolates were grown in Mueller-Hinton broth, a distinct biocidal spectrum was observed, indicating that the culture medium directly influences the production of antimicrobial compounds. Through molecular characterization of activity-positive isolates, they were identified as belonging to the genus Bacillus. The bioactive compounds produced by the Bacillus stratosphericus SS85 and SS69 strains were stable after storage time for three months. This is the first paper reporting on the antimicrobial activity of microorganisms isolated from coral Siderastrea stellata

II Diretriz Brasileira de Transplante Cardíaco

Universidade de São Paulo Faculdade de Medicina Hospital das ClínicasIIHospital de Messejana Dr. Carlos Alberto Studart GomesUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de MedicinaInstituto Dante Pazzanese de CardiologiaUniversidade Federal de Minas Gerais Hospital das ClínicasFaculdade de Medicina de São José do Rio PretoPontifícia Universidade Católica do ParanáIHospital Israelita Albert EinsteinInstituto Nacional de Cardiologia, Fundação Universitária do Rio Grande do Sul Instituto de CardiologiaReal e Benemérita Sociedade de Beneficência Portuguesa, São PauloHospital Pró-Cardíaco do Rio de JaneiroSanta Casa do Rio de JaneiroUNIFESP, EPMSciEL

Fermentation characteristics in hay from Cynodon and crop stubble treated with exogenous enzymes

ABSTRACT The effect of treatment with xylanase and β-glucanase was evaluated for gas production and the ruminal degradation of nutrients from the hay of Tifton 85 grass and the stubble of maize, sorghum, peanut, sunflower and sesame crops. Two commercial fibrolytic enzymes were used (Dyadic xylanase PLUS - Xylanase; BrewZyme LP-β-glucanase), added to the hay at doses of 7.5 units of endoglucanase and 0.46 units of xylanase per 500 mg/gDM, for the cellulase and xylanase products respectively. The chemical composition of the hay was determined for no enzyme application and 24 hours after enzyme treatment, and the in vitro gas production and in situ microbial degradation was estimated for dry matter, organic matter, neutral detergent fibre and truly-degradable organic matter after 24 hours of incubation in the rumen. Enzyme treatment of the hay from Tifton 85 grass and the stubble of maize, sorghum, sunflower, peanut and sesame crops with the exogenous fibrolytic enzymes β-glucanase and xylanase influences in vitro gas production, and the in situ degradation of dry matter, organic matter, neutral detergent fibre and truly-degradable organic matter in the rumen. This variation can be attributed to differences in the chemical composition of the hay from the grass and the crop stubble, and to the different ways the enzymes act upon the cell wall

Hydro alcoholic extract of pequi peel (Caryocar brasiliense) in rats submitted to the application of doxorubicin

Submitted by Franciele Moreira ([email protected]) on 2018-04-13T12:10:12Z No. of bitstreams: 2 Artigo - Léa Resende Moura - 2013.pdf: 1078713 bytes, checksum: bb3683939a17b6a4f54c551c30c2aad5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Approved for entry into archive by Luciana Ferreira ([email protected]) on 2018-04-13T12:35:13Z (GMT) No. of bitstreams: 2 Artigo - Léa Resende Moura - 2013.pdf: 1078713 bytes, checksum: bb3683939a17b6a4f54c551c30c2aad5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-04-13T12:35:13Z (GMT). No. of bitstreams: 2 Artigo - Léa Resende Moura - 2013.pdf: 1078713 bytes, checksum: bb3683939a17b6a4f54c551c30c2aad5 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2013-01O objetivo deste estudo foi avaliar o efeito antioxidante do extrato hidroalcoolico da casca do pequi (EHCP) em ratos após a administração de doxorrubicina (DOX). Foram utilizados ratos da raça Wistar, distribuídos em quatro grupos, sendo que os animais do G1 (n=6) receberam água e solução salina (grupo controle), G2 (n=7) EHCP e solução salina, G3 (n=7) água e DOX e G4 (n=6) EHCP e DOX. O EHCP foi administrado por gavagem durante 10 dias aos ratos dos grupos G2 e G4 e água aos dos G1 e G3. DOX na dose de 10mg kg-1 e solução salina 0,9% foram administradas por via intravenosa no dia sete após o início do experimento aos animais de G3 e G4 e aos de G1 e G2, respectivamente. Foram avaliados peso e taxa de mortalidade. Dez dias após o início do experimento, foi avaliada a concentração sérica de creatina quinase MB (CK-MB), troponina e mioglobina, e os ratos foram submetidos à eutanásia e à avaliação anatomopatológica. Não houve diferença entre os tratamentos quanto ao peso dos animais (P 0,05). Com relação à taxa de mortalidade, houve aumento no grupo 2 (P<0,05). Os resultados do teste qualitativo para a detecção de CK-MB, troponina I e mioglobina nos quatro grupos foram negativos e não foram observadas alterações macroscópicas nos órgãos dos ratos dos diferentes grupos. Constatou-se necrose tubular aguda multifocal de intensidade moderada a acentuada nas regiões cortical e medular nos rins de todos os ratos avaliados. A DOX em dose única de 10mg kg-1 e via intravenosa não promove alterações cardíacas em ratos e o EHCP nas condições avaliadas aumenta o índice de mortalidade em ratos, o que pode estar relacionado a substâncias tóxicas presentes na casca desse fruto.The objective of this study was to evaluate the antioxidant effect of the hydroalcoholic extract of pequi peel (HEPP) in rats after administration of doxorubicin (DOX). Were used 26 Wistar rats divided into four groups, which G1 (n=6) received water and saline solution (control group), G2 (n=7) HEPP and saline solution, G3 (n=7) water and DOX, and G4 (n=6) HEPP and DOX. The HEPP was administered by gavage for 10 days to G2 and G4 and water to G1 and G3. DOX and saline solution were administered intravenously on day seven after the start of the experiment, with the DOX (10mg kg-1) applied in G3 and G4, and saline solution 0.9% in G1 and G2. Were evaluated weight and mortality rate. Ten days after the start of the experiment were evaluated creatina kinase MB (CK-MB), troponin and myoglobin, and the rats were euthanized and evaluated morphologically. There was no difference between treatments in weight of animals (P>0.05). About the mortality rate an increase in group 2 was showed (P<0.05). The results of the qualitative test for the detection of CK-MB, troponin I and myoglobin in the four groups were negative and there were no macroscopic changes in different rat’s organs of different groups. Multifocal and moderate to severe acute tubular necrosis in cortical and medullary regions of the kidneys was observed in all rats studied. DOX intravenous and in a one dose of 10mg kg-1 don’t induce cardiac changes in rats and the HEPP in conditions here evaluated increase the rate of mortality of rats, which may be related to toxic substances in the peel of this fruit