Avanços na transformação genética de plantas mediada por Agrobacterium com ênfase em soja

Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.A soja é uma das maiores fontes de proteína vegetal da humanidade. É também muito importante na alimentação animal e como matéria-prima industrial. Grandes avanços foram recentemente alcançados em sua transformação genética. O objetivo desta revisão é fornecer uma discussão acerca de importantes fatores que influenciam a transformação de soja mediada por Agrobaterium, incluindo tecidos alvo, condições do tecido vegetal, métodos de injúria, sistemas de regeneração, marcadores de seleção e genes repórter

Chemical thinning of peach trees

Peach tree thinning is essential to harvest quality fruits and to keep balanced production for several years. Manual thinning of peach trees demands much work force and increases production costs. Chemical thinning may be an alternative solution, but efficiency depends on doses and species. This study aimed at evaluating the effect of metamitron – applied at different concentrations – on chemical thinning of peach trees in the south of Brazil. The experiment was carried out in a commercial peach tree orchard where ‘Sensação’ cultivars, in Morro Redondo, Rio Grande do Sul (RS) state, Brazil. The following metamitron concentrations were applied at concentrations of 100 mg L-1, 200 mg L-1, 300 mg L-1, 400 mg L-1, manual thinning was carried out 40 days after full bloom (DAFB) and to the treatment where the plants have no thinning. Fruit abscission, effective fructification, total number of plants per plant, production per plant, mean mass and fruit diameter were evaluated. Concentrations of 300 and 400 mg L-1 metamitron 40 DAFB provides promoted high fruit abscission, besides decreasing the number of fruits and production. Therefore, concentrations of 100 and 200 mg L-1 of metamitron 40 DAFB provides results in an efficient thinning practice similar to manual thinning for production, mass and fruit diameter

Comparison among chemical thinners applied to ‘Maciel’ and ‘Sensação’ peach trees

Peach tree thinning, which aims at reducing plant load so as to ensure productivity and fruit quality, has been manually carried out within a short period of time in the stage of fruit development. Due to the need and shortage of qualified man power, chemical thinning is one of the alternatives that can solve these difficulties found in manual thinning. This study aimed at evaluating the effect of different products which have been applied – either alone or in combination – to fruit thinning of peach tree cultivars ‘Sensação’ and ‘Maciel’ in Pelotas, Rio Grande do Sul (RS) state, Brazil. The experiment was conducted in the 2015/2016 crop in a commercial peach orchard located in Morro Redondo, RS, Brazil. The following seven treatments were carried out 40 days after full bloom (DAFB): plants with no thinning, manual thinning, metamitron, benzyladenine, benzyladenine + metamitron, ethephon, ethephon + metamitron. Fruit abscission, effective fructification, number of fruits and production per plant, mean mass and fruit classification into caliber classes, epidermis color, pulp firmness and soluble solids were evaluated. Production and number of fruits per plant decreased excessively, whereas fruits placed in categories of higher caliber increased when ethephon was either used alone or in combination with metamitron. When both metamitron and benzyladenine were applied, either alone or in combination, they led to fruit abscission and resulted in mean fruit size and weight, at harvest time, similar to those found in manual thinning. Application of chemical products – either alone or in combination – may be an alternative of peach tree thinning in orchard management

Qualidade de morangos submetidos à radiação UV-C pré-colheita

The objective of this work was to evaluate the influence of the use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry (Fragaria x ananassa) cultivation, on the physicochemical and phytochemical parameters of the fruits. The used experimental design was completely randomized, in a single-factor arrangement with 11, 19, 29, and 39 UV-C applications and without UV-C application. The hue of the fruits was smaller at the end of the study period. pH varied in strawberries that received 39 applications of UV-C, and the soluble solids content differed with 19 applications. Antioxidant activity was lower in fruits with 11, 19, and 29 UV-C applications, whereas vitamin C content was lower only in fruits that received 29 applications. The use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry cultivation, affects the physicochemical and phytochemical parameters of the fruits, but does not cause losses in their quality.O objetivo deste trabalho foi avaliar a influência do uso de radiação UV-C na intensidade de 0,53 KJ m-2, durante o cultivo de morango (Fragaria x ananassa), sobre os parâmetros físico-químicos e fitoquímicos dos frutos. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo unifatorial, com 11, 19, 29 e 39 aplicações de UV-C e sem aplicação de UV-C. A tonalidade dos frutos apresentou menor ângulo ao final do período de estudo. O pH variou em morangos que receberam 39 aplicações de UV-C, e o conteúdo de sólidos solúveis diferiu com 19 aplicações. A atividade antioxidante foi menor nas frutas com 11, 19 e 29 aplicações de UV-C, enquanto o teor de vitamina C foi menor apenas nas frutas que receberam 29 aplicações. O uso de radiação UV-C de 0,53 KJ m-2, durante o cultivo de morango, afeta os parâmetros físico-químicos e fitoquímicos dos frutos, mas não leva à perda de sua qualidade

Advances in Agrobacterium-mediated plant transformation with enphasys on soybean

Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes

Fluorescence and scanning electron microscopy of citrus sinensis protoplasts

The technology of protoplasts isolation is an important tool for studies of plasmatic membrane biochemistry and physiology and cell wall regeneration. Protoplasts of “Caipira” sweet orange (Citrus sinensis L. Osbeck) were enzymatically isolated from cells in suspension and subsequently cultured in liquid medium. This study aimed to evaluate the viability of protoplasts of “Caipira” sweet orange freshly isolated by the Fluorescein Diacetate (FDA) stain, which showed 95% of fluorescing protoplasts. Through the technique of scanning electron microscopy, it was observed protoplasts of “Caipira” sweet orange freshly isolated. It was used Calcofluor White (CFW) fluorescent stain to visualize the cell wall regenerated protoplasts after 8 days following culture of the above-mentioned variety, wherein was observed the cell wall through a bright blue ring intense fluorescence.