Isolation of a Novel Phage with Activity against Streptococcus mutans Biofilms

peer-reviewedStreptococcus mutans is one of the principal agents of caries formation mainly, because of its ability to form biofilms at the tooth surface. Bacteriophages (phages) are promising antimicrobial agents that could be used to prevent or treat caries formation by S. mutans. The aim of this study was to isolate new S. mutans phages and to characterize their antimicrobial properties. A new phage, ɸAPCM01, was isolated from a human saliva sample. Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5. In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml. Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties

Depolarisation cooling of an atomic cloud

We propose a cooling scheme based on depolarisation of a polarised cloud of trapped atoms. Similar to adiabatic demagnetisation, we suggest to use the coupling between the internal spin reservoir of the cloud and the external kinetic reservoir via dipolar relaxation to reduce the temperature of the cloud. By optical pumping one can cool the spin reservoir and force the cooling process. In case of a trapped gas of dipolar chromium atoms, we show that this cooling technique can be performed continuously and used to approach the critical phase space density for BECComment: 8 pages, 5 figure

Properties of neutral mesons in a hot and magnetized quark matter

The properties of non-interacting $\sigma$ and $\pi^{0}$ mesons are studied at finite temperature, chemical potential and in the presence of a constant magnetic field. To do this, the energy dispersion relations of these particles, including nontrivial form factors, are derived using a derivative expansion of the effective action of a two-flavor, hot and magnetized Nambu--Jona-Lasinio (NJL) model up to second order. The temperature dependence of the pole and screening masses as well as the directional refraction indices of magnetized neutral mesons are explored for fixed magnetic fields and chemical potentials. It is shown that, because of the explicit breaking of the Lorentz invariance by the magnetic field, the refraction index and the screening mass of neutral mesons exhibit a certain anisotropy in the transverse and longitudinal directions with respect to the direction of the external magnetic field. In contrast to their longitudinal refraction indices, the transverse indices of the neutral mesons are larger than unity.Comment: V1: 26 pages, 15 figures; V2: Discussions improved, references added. Version accepted for publication in PR

The diagnostic accuracy of CT and MRI for the detection of lymph node metastases in gallbladder cancer:A systematic review and meta-analysis

BACKGROUND: Lymph node metastases (LNM) are an ominous prognostic factor in gallbladder cancer (GBC) and, when present, should preclude surgery. However, uncertainty remains regarding the optimal imaging modality for pre-operative detection of LNM and international guidelines vary in their recommendations. The purpose of this study was to systematically review the diagnostic accuracy of computed tomography (CT) versus magnetic resonance imaging (MRI) in the detection of LNM of GBC. METHODS: A literature search of studies published until November 2017 concerning the diagnostic accuracy of CT or MRI regarding the detection of LNM in GBC was performed. Data extraction and risk of bias assessment was performed independently by two reviewers. The sensitivity of CT and MRI in the detection of LNM was reviewed. Additionally, estimated summary sensitivity, specificity and diagnostic accuracy of MRI were calculated in a patient based meta-analysis. RESULTS: Nine studies including 292 patients were included for narrative synthesis and 5 studies including 158 patients were selected for meta-analysis. Sensitivity of CT ranged from 0.25 to 0.93. Estimated summary diagnostic accuracy parameters of MRI were as follows: sensitivity 0.75 (95% CI 0.6 - 0.85), specificity 0.83 (95% CI 0.74 - 0.90), LR + 4.52 (95% CI 2.55-6.48) and LR- 0.3 (95% CI 0.15 - 0.45). Small (<10 mm) LNM were most frequently undetected on pre-operative imaging. Due to a lack of data, no subgroup analysis comparing the diagnostic accuracy of CT versus MRI could be performed. CONCLUSION: The value of current imaging strategies for the pre-operative assessment of nodal status in GBC remains unclear, especially regarding the detection of small LNM. Additional research is warranted in order to establish uniformity in international guidelines, improve pre-operative nodal staging and to prevent futile surgery

My mind is made up: Cancer concern and women’s preferences for contralateral prophylactic mastectomy

© 2019 John Wiley & Sons Ltd The fear of cancer recurrence is cited as a motivator of women's preferences between routine monitoring and contralateral prophylactic mastectomy (CPM) as methods of managing ongoing breast cancer risk. We conducted a discrete choice experiment among a general community sample of women who completed 12 hypothetical choices between routine monitoring and CPM described by aspects of treatment efficacy, safety, cost and involvement in decision-making. Respondents also completed a modified cancer worry question to assess cancer concern. Approximately 57.5% of 464 women always chose one option, typically routine monitoring. The majority (71.5%) reported being concerned about cancer recurrence when completing choice tasks. Latent class analysis identified three groups: preferred routine monitoring; preferred CPM; and “traders” (willing to swap between options). Among traders, women were less likely to choose an option associated with higher risk of recurrence. Women were more likely to choose options associated with less-intrusive monitoring methods and where they were involved in decision-making. Women concerned about cancer recurrence were more likely to choose CPM over monitoring. This study shows that women's preferences about how to manage breast cancer recurrence risk reflect the importance of the associated health effects, experience of care and attitudes to cancer recurrence

A novel topographic parameterization scheme indicates that martian gullies display the signature of liquid water

Martian gullies resemble gullies carved by water on Earth, yet are thought to have formed in an extremely cold (2-driven processes. That this argument persists demonstrates the limitations of morphological interpretations made from 2D images, especially when similar-looking landforms can form by very different processes. To overcome this we have devised a parameterization scheme, based on statistical discriminant analysis and hydrological terrain analysis of meter-scale digital topography data, which can distinguish between dry and wet surface processes acting on a landscape. Applying this approach to new meter-scale topographic datasets of Earth, the Moon and Mars, we demonstrate that martian gullied slopes are dissimilar to dry, gullied slopes on Earth and the Moon, but are similar to both terrestrial debris flows and fluvial gullies. We conclude that liquid water was integral to the process by which martian gullies formed. Finally, our work shows that quantitative 3D analyses of landscape have great potential as a tool in planetary science, enabling remote assessment of processes acting on planetary surfaces

Statistical properties of random matrix product states

We study the set of random matrix product states (RMPS) introduced in arXiv:0908.3877 as a tool to explore foundational aspects of quantum statistical mechanics. In the present work, we provide an accurate numerical and analytical investigation of the properties of RMPS. We calculate the average state of the ensemble in the non-homogeneous case, and numerically check the validity of this result. We also suggest using RMPS as a tool to approximate properties of general quantum random states. The numerical simulations presented here support the accuracy and efficiency of this approximation. These results suggest that any generalized canonical state can be approximated with high probability by the reduced density matrix of a random MPS, if the average MPS coincide with the associated microcanonical ensemble.Comment: 12 pages, 17 figures; published versio