Searching for interacting QTL in related populations of an outbreeding species

Many important crop species are outbreeding. In outbreeding species the search for genes affecting traits is complicated by the fact that in a single cross up to four alleles may be present at each locus. This paper is concerned with the search for interacting quantitative trait loci (QTL) in populations which have been obtained by crossing a number of parents. It will be assumed that the parents are unrelated, but the methods can be extended easily to allow a pedigree structure. The approach has two goals: (1) finding QTL that are interacting with other loci and also loci which behave additively; (2) finding parents which segregate at two or more interacting QTL. Large populations obtained by crossing these parents can be used to study interactions in detail. QTL analysis is carried out by means of regression on predictions of QTL genotypes

О подборе материала профессиональной направленности на занятиях по немецкому языку в группах факультета искусств

Цель статьи показать ряд методических приемов профессиональной направленности для проведения занятий по немецкому языку в группах факультета искусств

Sickness certification in primary care: a survey on views and practices among Swiss physicians.

QUESTIONS UNDER STUDY: Studies from several countries (Scandinavia, United Kingdom) report that general practitioners (GPs) experience problems in sickness certification. Our study explored views of Swiss GPs towards sickness certification, their practice and experience, professional skills and problematic interactions with patients. METHODS: We conducted an online survey among GPs throughout Switzerland, exploring behaviour of physicians, patients and employers with regard to sickness certification; GPs' views about sickness certification; required competences for certifying sickness absence, and approaches to advance their competence. We piloted the questionnaire and disseminated it through the networks of the five Swiss academic institutes for primary care. RESULTS: We received 507 valid responses (response rate 50%). Only 43/507 GPs experienced sickness certification as problematic per se, yet 155/507 experienced problems in sickness certification at least once a week. The 507 GPs identified estimating a long-term prognosis about work capacity (64%), handling conflicts with patients (54%), and determining the reduction of work capacity (42%) as problematic. Over 75% would welcome special training opportunities, e.g., on sickness certifications during residency (93%), in insurance medicine (81%), and conflict management (80%). CONCLUSION: Sickness certification as such does not present a major problem to Swiss GPs, which contrasts with the experience in Scandinavian countries and in the UK. Swiss GPs did identify specific tasks of sickness certification as problematic. Training opportunities on sick-leave certification and insurance medicine in general were welcomed

Changes in need-supportive teaching over the course of one school year: differences between students with special educational needs and typically developing students

According to self-determination theory, need-supportive teaching is an important aspect of teacher-student interactions. It involves supporting the basic psychological needs of autonomy, competence, and relatedness. In this study, we observed need-supportive teaching in teacher-student interactions with students with special educational needs (SEN) and typically developing students in pre-vocational secondary education. Need-supportive teaching was coded in teacher-student interactions in 59 lessons across 7 classes. Teachers interacted just as often with students with SEN as they did with students without SEN. Multilevel analysis indicates that teachers provided higher levels of need support to students with behavioural problems. Autonomy support increased slightly throughout the school year. The provision of structure was erratic at first, with the highest level halfway through the school year and the lowest at the end. Involvement declined halfway through the school year and stabilised thereafter. These patterns were largely the same for students with SEN, albeit with more fluctuations between lessons

American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Score: A study protocol for the translation and validation of the Dutch language version

Introduction: The American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Score is among the most commonly used instruments for measuring the outcome of treatment in patients who sustained a complex ankle or hindfoot injury. It combines a clinician-reported and a patient-reported part. A valid Dutch version of this instrument is currently not available. Such a translated and validated instrument would allow objective comparison across hospitals or between patient groups, and with shown validity and reliability it may become a quality of care indicator in future. The main aims of this study are to translate and culturally adapt the AOFAS Ankle-Hindfoot Score questionnaire into Dutch according to international guidelines, and to evaluate the measurement properties of the AOFAS Ankle-Hindfoot Score-Dutch language version (DLV) in patients with a unilateral ankle or hindfoot fracture. Methods and analysis: The design of the study will be a multicentre prospective observational study (case series) in patients who presented to the emergency department with a unilateral ankle or hindfoot fracture or (fracture) dislocation. A research physician or research assistant will complete the AOFAS Ankle-Hindfoot Score-DLV based on interview for the subjective part and a physical examination for the objective part. In addition, patients will be asked to complete the Foot Function Index (FFI) and the Short Form-36 (SF-36). Descriptive statistics (including floor and ceiling effects), internal consistency, construct validity, reproducibility (ie, test-retest reliability, agreement and smallest detectable change) and responsiveness will be assessed for the AOFAS DLV. Ethics and dissemination: This study has been exempted by the Medical Research Ethics Committee (MREC) Erasmus MC (Rotterdam, the Netherlands). Each participant will provide written consent to participate and remain anonymised during the study. The results of the study are planned to be published in an international, peer-reviewed journal. Trial registration number: NTR5613. pre-result

Association between epicardial adipose tissue and cardiac dysfunction in subjects with severe obesity

Aim: Epicardial adipose tissue (EAT) plays a role in obesity-related heart failure with preserved ejection fraction. However, the association of EAT thickness with the development of cardiac dysfunction in subjects with severe obesity without known cardiovascular disease is unclear. The aim of this study was to determine the association between EAT thickness and cardiac dysfunction and describe the potential value of EAT as an early marker of cardiac dysfunction. Methods and results: Subjects with body mass index ≥35 kg/m2 aged 35 to 65 years, who were referred for bariatric surgery, without suspicion of or known cardiac disease, were enrolled. Conventional transthoracic echocardiography and strain analyses were performed. A total of 186 subjects were divided into tertiles based on EAT thickness, of whom 62 were in EAT-1 (EAT &lt;3.8 mm), 63 in EAT-2 (EAT 3.8–5.4 mm), and 61 in EAT-3 (EAT &gt;5.4 mm). Parameters of systolic and diastolic function were comparable between tertiles. Patients in EAT-3 had the lowest global longitudinal strain (GLS) and left atrial contractile strain (LASct). Linear regression showed that a one-unit increase in EAT thickness (mm) was independently associated with a decrease in GLS (%) (β coefficient −0.404, p = 0.002), and a decrease in LASct (%) (β coefficient −0.544, p = 0.027). Furthermore, EAT-3 independently predicted cardiac dysfunction as defined by a GLS &lt;18% (odds ratio 2.8, p = 0.013) and LASct &lt;14% (odds ratio 2.5, p = 0.045). Conclusions: Increased EAT thickness in subjects with obesity without known cardiac disease was independently associated with subclinical cardiac dysfunction. Our findings suggest that EAT might play a role in the early stages of cardiac dysfunction in obesity before this may progress to overt clinical disease.</p

The American Orthopaedic Foot and Ankle Society Ankle-Hindfoot Scale; Translation and validation of the Dutch language version for ankle fractures

Objectives The American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scale is among the most commonly used instruments for measuring outcome of treatment in patients who sustained a complex ankle or hindfoot injury. It consists of a patient-reported and a physician-reported part. A validated, Dutch version of this instrument is currently not available. The aim of this study was to translate the instrument into Dutch and to determine the measurement properties of the AOFAS Ankle-Hindfoot Scale Dutch language version (DLV) in patients with a unilateral ankle fracture. Setting Multicentre (two Dutch hospitals), prospective observational study. Participants In total, 142 patients with a unilateral ankle fracture were included. Ten patients were lost to follow-up. Primary and secondary outcome measures Patients completed the subjective (patient-reported) part of the AOFAS Ankle-Hindfoot Scale-DLV. A physician or trained physician-assistant completed the physician-reported part. For comparison and evaluation of the measuring characteristics, the Foot Function Index and the Short Form-36 were completed by the patient. Descriptive statistics (including floor and ceiling effects), reliability (ie, internal consistency), construct validity, reproducibility (ie, test-retest reliability, agreement and smallest detectable change) and responsiveness were determined. Results The AOFAS-DLV and its subscales showed good internal consistency (Cronbach's α >0.90). Construct validity and longitudinal validity were proven to be adequate (76.5% of predefined hypotheses were confirmed). Floor effects were not present. Ceiling effects were present from 6 months onwards, as expected. Responsiveness was adequate, with a smallest detectable change of 12.0 points. Conclusions The AOFAS-DLV is a reliable, valid and responsive measurement instrument for evaluating functional outcome in patients with a unilateral ankle fracture. This implies that the questionnaire is suitable to compare different treatment modalities within this population or to compare outcome across hospitals. Trial registration The Netherlands Trial Register (NTR5613

Validation of the American Orthopaedic Foot and Ankle Society Ankle-Hindfoot Scale Dutch language version in patients with hindfoot fractures

OBJECTIVES: The American Orthopaedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot Scale is among the most used questionnaires for measuring functional recovery after a hindfoot injury. Recently, this instrument was translated and culturally adapted into a Dutch version. In this study, the measurement properties of the Dutch language version (DLV) were investigated in patients with a unilateral hindfoot fracture.DESIGN: Multicentre, prospective observational study. SETTING: This multicentre study was conducted in three Dutch hospitals. PARTICIPANTS: In total, 118 patients with a unilateral hindfoot fracture were included. Three patients were lost to follow-up. PRIMARY AND SECONDARY OUTCOME MEASURES: Patients were asked to complete the AOFAS-DLV, the Foot Function Index and the Short Form-36 on three occasions. Descriptive statistics (including floor and ceiling effects), reliability (ie, internal consistency), construct validity, reproducibility (ie, test-retest reliability, agreement and smallest detectable change (SDC)) and responsiveness were determined. RESULTS: Internal consistency was inadequate for the AOFAS-DLV total scale (α=0.585), but adequate for the function subscale (α=0.863). The questionnaire had adequate construct validity (82.4% of predefined hypotheses were confirmed), but inadequate longitudinal validity (70.6%). No floor effects were found, but ceiling effects were present in all AOFAS-DLV (sub)scales, most pronounced from 6 to 24 months after trauma onwards. Responsiveness was only adequate for the pain and alignment subscales, with a SDC of 1.7 points. CONCLUSIONS: The AOFAS Ankle-Hindfoot Scale DLV has adequate construct validity and is reliable, making it a suitable instrument for cross-sectional studies investigating functional outcome in patients with a hindfoot fracture. The inadequate longitudinal validity and responsiveness, however, hamper the use of the questionnaire in longitudinal studies and for assessing long-term functional outcome. TRIAL REGISTRATION NUMBER: NTR5613; Post-results

Reducing cardiometabolic risk in adults with a low socioeconomic position: protocol of the Supreme Nudge parallel cluster-randomised controlled supermarket trial

This erratum describes changes made in our previously published study protocol [1], as the occurrence of the COVID-19 pandemic has led to insurmountable challenges in feasibility to maintain the original design. The planned start of participant recruitment for the trial coincided with the first COVID-19 lockdown in the Netherlands starting March 16 2020. As a result, the start of the study was postponed by 11 months. The continued/renewed lockdown hampered face-to-face contact and thus some planned physical measurements. These circumstances required adaptation to remote data collection methods, revision of recruitment goals and of the primary study outcomes, and inclusion of additional study sites to secure adequate participant inclusion rates. Therefore, below we present the revised methodology of the Supreme Nudge parallel cluster-randomised controlled supermarket trial. All changes made to the original protocol were reviewed and approved by the Medical Ethics Review Committee of VU University Medical Center (reference number: 2019.334) prior to implementation