Pro-oxidant Activity And Genotoxicity Of The Astronium Fraxinifolium Using Wing Smart And Allium Cepa Test

Astronium fraxinifolium is an arboreal species found throughout the Brazilian Cerrado region and used in folk medicine as antimicrobial, anti-hemorrhagic and healing. Pro-oxidant activity of extracts of fraxinifolium rough Relative Electrophoresis Mobility (REM) of Bovine Serum Albumin (BSA) protein in presence of the extract and Cu2+ and also the genotoxic potential through Somatic Mutation and Recombination Test (SMART) and Allium cepa tests has been investigated during this study. In the REM, the extracts acting exclusively on BSA resulted in a band formation with higher molecular weight than BSA, probably due to oxidative action of the extracts. In presence of Cu2+and extracts occurred protein fragmentation due to Cu2+ oxidative action potentiated by the extracts. In SMART test the frequency of mutant spots increases with the increased concentration of extract A. fraxinifolium (50 mg mL-1= 1.40 and 100 mg mL-1 = 2.66). The HB cross shows a decrease in the total of mutant spots frequency for the different treatments (50 mg mL-1 =6.60 and 100 mg mL-1 = 3.25). Evaluation of A cutest demonstrated the following results (concentration extract = chromosomal abnormalities) 1 mg mL-1 = 85,10 mg L-1 = 61 50 mg mL-1 = 53,100 mg mL-1 =33 and MMS 10 mg L-1 = 50. Genotoxic and cytotoxic actions can be explained by the actions of tannins present in its composition. Butthere may be other substances that also act for such results. The genotoxicity in medicinal plants contributes to therapeutic safety warning that although the use of medicinal plants is an inexpensive and non-aggressive method it can cause harmful effects if used incorrectly. © 2016 Rafael Rozolen Teixeria Zafred et al.10427628

In silico ADMET prediction, evaluation of cytotoxicity in mouse splenocytes and preliminary evaluation of in vitro antimalarial activity of 4-(4-chlorophenyl)thiazole compounds

Abstract In this work, an in silico study and evaluation of the cytotoxicity of 4-(4-chlorophenyl)thiazole compounds against mouse splenocytes and the chloroquine-sensitive Plasmodium falciparum 3D7 strain are reported. The in silico results showed that the compounds have important pharmacokinetic properties for compounds with potential drug candidates. Regarding cytotoxicity assays against splenocytes, the compounds have low cytotoxicity. In addition, they were able to promote activation of these cells by increasing nitric oxide production without promoting cell death. Finally, they were able to promote cell proliferation. Regarding the in vitro anti-P. falciparum activity assays, it was observed that the compounds were able to inhibit the parasite’s growth, presenting IC50 values ranging from 0.79 to greater than 10 µM. These results are promising when compared to chloroquine. Therefore, this study showed that 4-(4-chlorophenyl)thiazole compounds are promising candidates for antimalarials

Relationship between levels of blood metabolites and wool quality

The type of feeding directly alters the levels of blood metabolites and the characteristics of wool. In this sense, the objective of this work is to relate the levels of blood metabolites to the quality of wool. 25 three-year-old Corriedale breed ewes were used in a 98 days trial period, during which the levels of blood metabolites and the objectives data of wool quality were evaluated. For the objective wool quality analysis there was a decrease in the average fiber diameter (from the start to the end of the experiment) and daily average wool staple growth of 0.40 mm. The only existing correlation was found between serum glucose levels and the change in the average diameter of the fibers, the variation in the length of wool staple and the variation of the diameter at the point where values were higher. Blood variables related to albumin and total plasma protein had no significant correlation with the wool quality measurements.O tipo de alimentação altera diretamente os níveis dos metabólitos sanguíneos e as características da lã. Neste sentido o objetivo deste trabalho é relacionar os níveis de metabólitos sanguíneos com a qualidade da lã. Foram utilizadas 25 ovelhas com três anos de idade da raça Corriedale, em um período experimental de 98 dias, onde se avaliou os níveis dos metabolitos sanguíneos e os dados objetivos de qualidade de lã. Para as análises objetivas de qualidade de lã ocorreu uma diminuição no diâmetro médio das fibras (do inicio ao fim do experimento) e um crescimento diário médio da mecha de lã de 0,40 mm. Somente foi encontrada correlação entre os níveis séricos de glicose com a variação do diâmetro médio das fibras, a variação do comprimento da mecha de lã e com a variação do diâmetro no ponto em que apresentou maiores valores. As variáveis sanguíneas referentes à albumina e proteínas plasmáticas totais não tiveram correlação significativa com as aferições de qualidade de lã